Chance 1955 J Biol Chem-I
|Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217:383-93.|
In the course of spectrophotometric studies of the kinetics and nature of the respiratory enzyme systems involved in oxidative phosphorylation (1) it has been essential to assay simultaneously the changes of respiratory rate caused by the presence and absence of phosphate acceptor. A vibrating platinum electrode inserted in the optical cuvette exhibits the necessary accuracy and speed of response. It is found that the initiation and cessation of rapid respiration due to the oxidative phosphorylation of added phosphate acceptor are abrupt enough to mark accurately the amount of oxygen uptake in times as short as 10 to 20 seconds. Thus phosphorylative activity can be directly calculated as the quotient of added ADP to the amount of rapid oxygen utilization. Typical values for the ADP:O ratio are given for various substrates and possible errors and limitations of the method are discussed.
Labels: MiParea: Respiration
Organism: Rat, Guinea pig Tissue;cell: Liver Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS Pathway: N