Brunetta 2019 J Funct Foods
|Brunetta HS, de Paula GC, Fritzen M, Cecchini MS, dos Santos GJ, Nazari EM, Rafacho A, de Bem AF, Nunes EA (2019) Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice. J Funct Foods 62:103544.|
Abstract: Leucine is an essential amino acid that has been investigated by its participation in the regulation of whole-body metabolism and mitochondrial function. Here, we evaluated acute leucine effects on mitochondrial respiration of skeletal muscle from male Swiss mice in vitro. Additionally, we further investigated the effects of 4-wk leucine ingestion (2.5% on drinking water) on skeletal muscle mitochondrial respiration and morphology of diet-induced obesity (DIO) mice. In vitro, acute leucine increased mitochondrial respiration, and these effects were abolished in the presence of rapamycin. In DIO mice, ingestion of leucine for 4-wk improved glucose tolerance and insulin responsivity. Leucine supplementation also prevented the reduction in mitochondrial respiration, size, and complexity in the soleus skeletal muscle. We conclude that the positive effects of leucine on whole-body metabolism in DIO mice are associated with improvements in skeletal muscle mitochondrial function and morphology. Furthermore, leucine acute effects on mitochondrial respiration are mTORC1 dependent.
• Keywords: Leucine, Obesity, Mitochondria, Insulin resistance, High-fat diet • Bioblast editor: Plangger M
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Pharmacology;toxicology Pathology: Obesity
Organism: Mouse Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Regulation: Amino acid Coupling state: LEAK, OXPHOS Pathway: N, S, NS, ROX HRR: Oxygraph-2k
Labels, 2019-10, Rapamycin