https://wiki.oroboros.at/api.php?action=feedcontributions&user=Doerrier+Carolina&feedformat=atomBioblast - User contributions [en]2024-03-28T13:12:40ZUser contributionsMediaWiki 1.36.1https://wiki.oroboros.at/index.php?title=Doerrier_Carolina&diff=233216Doerrier Carolina2022-10-20T12:21:33Z<p>Doerrier Carolina: </p>
<hr />
<div>{{EAGLE<br />
|COST= Member<br />
|COST WG1 = WG1<br />
|COST WG2 = WG2<br />
|COST WG3 = WG3<br />
|COST WG4 = WG4<br />
|COST ECI= ECI<br />
}}<br />
: [[Management_Committee_MitoEAGLE#MC_Substitutes|MC Substitute]] - [[Management Committee MitoEAGLE]]<br />
{{NextGen-O2k H2020-support}}<br />
{{Person<br />
|lastname=Doerrier Velasco<br />
|firstname=Carolina<br />
|title=PhD.<br />
|institution=::::::::::::::::[[File:DoerrierC.JPG|right|150px|Carolina Doerrier]]<br />
<br />
<br />
<br />
<br />
Background: [[ES Granada Acuna-Castroviejo D]]<br />
<br />
Carolina was part of the [[Oroboros_Contact |Oroboros Team]] from October 2014 to May 2022.<br />
|address=<br />
|area code=<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress=carolina.doerrier@gmail.com<br />
|weblink=[https://www.researchgate.net/profile/Carolina_Doerrier_Velasco ResearchGate profile], ORCID:[[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]], [[Bioblast|www.bioblast.at]]<br />
}}<br />
<br />
__TOC__<br />
== Bioenergetics Communications ==<br />
::::* Carolina Doerrier is Section Editor of the journal '''[[Bioenergetics Communications]]'''<br />
:::::'''Keywords:''' O2k-FluoRespirometry, oxidative stress, permeabilized muscle fibers, NAD(P)H, sepsis, aging, melatonin<br />
==[[Oroboros Contact| Oroboros-team]]==<br />
:* Previous laboratory: [[ES Granada Acuna-Castroviejo D]], [[AT Innsbruck Oroboros]].<br />
:* Collaboration with the [[AT_Innsbruck_Gnaiger E|Gnaiger Lab]] in Innsbruck during a 3-months scholarship (Sep - Dec 2011), in the frame of the K-Regio project ''[[MitoCom_O2k-Fluorometer| MitoCom Tyrol]]''.<br />
:* Collaboration with the [[US FL Orlando Goodpaster BH|Goodpaster Lab]] in Orlando (2017).<br />
:* MitoEAGLE Short-Term Scientific Mission at [[ES Barcelona Garcia-Roves PM]] in Barcelona (2018).<br />
:* Guest tutor at [[IOC65]] and [[IOC68]]. <br />
:* Tutor at [[IOC100]], [[IOC104]], [[IOC106]], [[IOC110]], [[IOC112]], [[IOC114]], [[IOC115]], [[IOC116]], [[IOC120]], [[IOC122]], [[IOC130]], [[IOC132]], [[IOC133]], [[IOC137]], [[IOC139]], [[IOC144]] and [[IOC145]].<br />
<br />
== Bioblast Editorial Board ==<br />
<br />
:* Bioblast national editor [[MiPNet Laboratories Spain]]<br />
:* [[Library of protocols]]<br />
<br />
== MitoEAGLE Short-Term Scientific Mission ==<br />
****: [[Short-Term Scientific Missions MitoEAGLE#STSM_Grant_Period_2 |STSM Grant Period 2]]<br />
::: '''Work Plan summary'''<br />
:::: WORKPLAN SUMMARY Different experimental aspects (e.g. purity of the preparations, environmental humidity which could affect the wet weight of the muscle, chemicals) will be evaluated during the STSM in order to clarify the sources of variability found in the MitoEAGLE WG2 pilot study. The preparation of the samples will be performed according the SOP established for the MitoEAGLE WG2 pilot study. Permeabilized fibers from soleus of C57BL6/J mice will be used. Mitochondrial respiration will be assessed by O2k high-resolution respirometry (HRR). The Substrate- Uncoupler-Inhibitor Titration (SUIT) protocol used in the MitoEAGLE WG2 pilot study for obtaining mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers, will be used to perform the experiments during the STSM at the Department of Physiological Sciences II in the Faculty of Medicine (Barcelona University, Barcelona, Spain).<br />
<br />
<br />
<br />
== Participated at ==<br />
::::* [[MiPNet 26.16 NextGen-O2k Summit 2021 Virtual]]<br />
::::* [[2020 PaduaMuscleDays Padua IT]]<br />
::::* [[FAT4BRAIN School IOC147 Virtual Event]]<br />
::::* [[MiPNet25.06 IOC145 Innsbruck AT|IOC145 Innsbruck AT]]<br />
::::* [[MiPNet25.03 IOC144 Innsbruck AT|IOC144 Innsbruck AT]]<br />
::::* [[MiP2019/MitoEAGLE Belgrade RS|MitoEAGLE 2019 Belgrade RS]]<br />
::::* [[MiP2019/MitoEAGLE Belgrade RS]]<br />
::::* [[FEBS Workshop Ageing 2019 Innsbruck AT]]<br />
::::* [[Mitochondrial Physiology ‐ from Organelle to Organism 2019 Copenhagen DK]]<br />
::::* [[MiPschool Coimbra 2019|MiP/MitoEAGLE Training School 2019 Coimbra PT]]<br />
::::* [[IOC139| IOC139 Schroecken AT]]<br />
::::* [[Life Science PhD Meeting 2019 Innsbruck AT]]<br />
::::* [[MitoEAGLE Obergurgl 2019-01-31| MitoEAGLE 2019 Obergurgl AT]]<br />
::::* [[IOC137|IOC137 Innsbruck AT]]<br />
::::* [[MitoEAGLE_Innsbruck_2018-11-19| MitoEAGLE 2018 Innsbruck AT]]<br />
::::* [[IOC133|IOC133 Innsbruck AT]]<br />
::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]<br />
::::* [[EBEC2018 Budapest HU]]<br />
::::* [[MitoEAGLE Copenhagen 2018| MitoEAGLE 2018 Copenhagen DK]]<br />
::::* [[MiPNet23.06 IOC130 Schroecken AT| IOC130 Schroecken AT]]<br />
::::* [[ESCI 2018 Barcelona ES]]<br />
::::* [[MitoFit Workshop ATP 2017 Innsbruck AT]]<br />
::::* [[MiP2017/MitoEAGLE Hradec Kralove CZ|MitoEAGLE 2017 Hradec Kralove CZ]]<br />
::::* [[MiPNet22.01 IOC122 Schroecken AT]]<br />
::::* [[MitoEAGLE Obergurgl 2017| MitoEAGLE 2017 Obergurgl AT]]<br />
::::* [[MiPschool Obergurgl 2017| MiPschool 2017 Obergurgl AT]]<br />
::::* [[MitoEAGLE Barcelona 2017| MitoEAGLE 2017 Barcelona ES]]<br />
::::* [[MiPNet22.04 IOC120 Barcelona ES | IOC120 Barcelona ES]]<br />
::::* [[Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE| Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE]]<br />
::::* [[MiPNet21.11 IOC116 Innsbruck AT|IOC116 Innsbruck AT]]<br />
::::* [[IOC115 | IOC115 Schroecken AT]]<br />
::::* [[MiPNet21.19 IOC114 Innsbruck AT | IOC114 Innsbruck AT]]<br />
::::* [[MitoEAGLE Verona 2016| MitoEAGLE 2016 Verona IT]]<br />
::::* [[MitoFit Science Camp 2016 Kuehtai AT]]<br />
::::* [[MiPNet21.15 IOC112 Kuehtai AT|IOC112 Kuehtai AT]]<br />
::::* [[MiPNet21.04 IOC110 Melbourne AU|IOC110 Melbourne AU]]<br />
::::* [[Research to Practice 2016 Melbourne AU]]<br />
::::* [[Bioblast 2012 | Bioblast 2012 Innsbruck AT]]<br />
::::* [[MiPNet20.10 IOC106 Schroecken|IOC106 Schroecken AT]]<br />
::::* [[MiPNet20.05 IOC104 Greenville |IOC104 Greenville NC US]]<br />
::::* [[IOC100 | IOC100 Schroecken AT]]<br />
::::* [[IOC68 | IOC68 Schroecken AT]]<br />
::::* [[IOC65 | IOC65 Schroecken AT]]<br />
::::* [[MiPNet14.04 IOC51 | IOC51 Schroecken AT]]<br />
::::* [[MiP2015 | MiP2015 Pec pod Snezkou CZ]]<br />
::::* [[MiPschool Greenville 2015 | MiPschool 2015 Greenville NC US]]<br />
<br />
{{Labelingperson<br />
|field of research=Basic<br />
|topics=[[High-resolution respirometry]], [[Mitochondria]], [[Melatonin]], [[Reactive oxygen species]], [[Reactive nitrogen species]], [[Sepsis]]<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Doerrier_Carolina&diff=233141Doerrier Carolina2022-10-18T17:54:25Z<p>Doerrier Carolina: </p>
<hr />
<div>{{EAGLE<br />
|COST= Member<br />
|COST WG1 = WG1<br />
|COST WG2 = WG2<br />
|COST WG3 = WG3<br />
|COST WG4 = WG4<br />
|COST ECI= ECI<br />
}}<br />
: [[Management_Committee_MitoEAGLE#MC_Substitutes|MC Substitute]] - [[Management Committee MitoEAGLE]]<br />
{{NextGen-O2k H2020-support}}<br />
{{Person<br />
|lastname=Doerrier Velasco<br />
|firstname=Carolina<br />
|title=PhD.<br />
|institution=::::::::::::::::[[File:DoerrierC.JPG|right|150px|Carolina Doerrier]]<br />
<br />
<br />
<br />
<br />
Background: [[ES Granada Acuna-Castroviejo D]]<br />
<br />
Carolina was part of the [[Oroboros_Contact |Oroboros Team]] from October 2014 to May 2022.<br />
|address=Schoepfstrasse 18<br />
|area code=6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress=carolina.doerrier@gmail.com<br />
|weblink=[https://www.researchgate.net/profile/Carolina_Doerrier_Velasco ResearchGate profile], ORCID:[[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]], [[Bioblast|www.bioblast.at]]<br />
}}<br />
<br />
__TOC__<br />
== Bioenergetics Communications ==<br />
::::* Carolina Doerrier is Section Editor of the journal '''[[Bioenergetics Communications]]'''<br />
:::::'''Keywords:''' O2k-FluoRespirometry, oxidative stress, permeabilized muscle fibers, NAD(P)H, sepsis, aging, melatonin<br />
==[[Oroboros Contact| Oroboros-team]]==<br />
:* Previous laboratory: [[ES Granada Acuna-Castroviejo D]], [[AT Innsbruck Oroboros]].<br />
:* Collaboration with the [[AT_Innsbruck_Gnaiger E|Gnaiger Lab]] in Innsbruck during a 3-months scholarship (Sep - Dec 2011), in the frame of the K-Regio project ''[[MitoCom_O2k-Fluorometer| MitoCom Tyrol]]''.<br />
:* Collaboration with the [[US FL Orlando Goodpaster BH|Goodpaster Lab]] in Orlando (2017).<br />
:* MitoEAGLE Short-Term Scientific Mission at [[ES Barcelona Garcia-Roves PM]] in Barcelona (2018).<br />
:* Guest tutor at [[IOC65]] and [[IOC68]]. <br />
:* Tutor at [[IOC100]], [[IOC104]], [[IOC106]], [[IOC110]], [[IOC112]], [[IOC114]], [[IOC115]], [[IOC116]], [[IOC120]], [[IOC122]], [[IOC130]], [[IOC132]], [[IOC133]], [[IOC137]], [[IOC139]], [[IOC144]] and [[IOC145]].<br />
<br />
== Bioblast Editorial Board ==<br />
<br />
:* Bioblast national editor [[MiPNet Laboratories Spain]]<br />
:* [[Library of protocols]]<br />
<br />
== MitoEAGLE Short-Term Scientific Mission ==<br />
****: [[Short-Term Scientific Missions MitoEAGLE#STSM_Grant_Period_2 |STSM Grant Period 2]]<br />
::: '''Work Plan summary'''<br />
:::: WORKPLAN SUMMARY Different experimental aspects (e.g. purity of the preparations, environmental humidity which could affect the wet weight of the muscle, chemicals) will be evaluated during the STSM in order to clarify the sources of variability found in the MitoEAGLE WG2 pilot study. The preparation of the samples will be performed according the SOP established for the MitoEAGLE WG2 pilot study. Permeabilized fibers from soleus of C57BL6/J mice will be used. Mitochondrial respiration will be assessed by O2k high-resolution respirometry (HRR). The Substrate- Uncoupler-Inhibitor Titration (SUIT) protocol used in the MitoEAGLE WG2 pilot study for obtaining mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers, will be used to perform the experiments during the STSM at the Department of Physiological Sciences II in the Faculty of Medicine (Barcelona University, Barcelona, Spain).<br />
<br />
<br />
<br />
== Participated at ==<br />
::::* [[MiPNet 26.16 NextGen-O2k Summit 2021 Virtual]]<br />
::::* [[2020 PaduaMuscleDays Padua IT]]<br />
::::* [[FAT4BRAIN School IOC147 Virtual Event]]<br />
::::* [[MiPNet25.06 IOC145 Innsbruck AT|IOC145 Innsbruck AT]]<br />
::::* [[MiPNet25.03 IOC144 Innsbruck AT|IOC144 Innsbruck AT]]<br />
::::* [[MiP2019/MitoEAGLE Belgrade RS|MitoEAGLE 2019 Belgrade RS]]<br />
::::* [[MiP2019/MitoEAGLE Belgrade RS]]<br />
::::* [[FEBS Workshop Ageing 2019 Innsbruck AT]]<br />
::::* [[Mitochondrial Physiology ‐ from Organelle to Organism 2019 Copenhagen DK]]<br />
::::* [[MiPschool Coimbra 2019|MiP/MitoEAGLE Training School 2019 Coimbra PT]]<br />
::::* [[IOC139| IOC139 Schroecken AT]]<br />
::::* [[Life Science PhD Meeting 2019 Innsbruck AT]]<br />
::::* [[MitoEAGLE Obergurgl 2019-01-31| MitoEAGLE 2019 Obergurgl AT]]<br />
::::* [[IOC137|IOC137 Innsbruck AT]]<br />
::::* [[MitoEAGLE_Innsbruck_2018-11-19| MitoEAGLE 2018 Innsbruck AT]]<br />
::::* [[IOC133|IOC133 Innsbruck AT]]<br />
::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]<br />
::::* [[EBEC2018 Budapest HU]]<br />
::::* [[MitoEAGLE Copenhagen 2018| MitoEAGLE 2018 Copenhagen DK]]<br />
::::* [[MiPNet23.06 IOC130 Schroecken AT| IOC130 Schroecken AT]]<br />
::::* [[ESCI 2018 Barcelona ES]]<br />
::::* [[MitoFit Workshop ATP 2017 Innsbruck AT]]<br />
::::* [[MiP2017/MitoEAGLE Hradec Kralove CZ|MitoEAGLE 2017 Hradec Kralove CZ]]<br />
::::* [[MiPNet22.01 IOC122 Schroecken AT]]<br />
::::* [[MitoEAGLE Obergurgl 2017| MitoEAGLE 2017 Obergurgl AT]]<br />
::::* [[MiPschool Obergurgl 2017| MiPschool 2017 Obergurgl AT]]<br />
::::* [[MitoEAGLE Barcelona 2017| MitoEAGLE 2017 Barcelona ES]]<br />
::::* [[MiPNet22.04 IOC120 Barcelona ES | IOC120 Barcelona ES]]<br />
::::* [[Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE| Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE]]<br />
::::* [[MiPNet21.11 IOC116 Innsbruck AT|IOC116 Innsbruck AT]]<br />
::::* [[IOC115 | IOC115 Schroecken AT]]<br />
::::* [[MiPNet21.19 IOC114 Innsbruck AT | IOC114 Innsbruck AT]]<br />
::::* [[MitoEAGLE Verona 2016| MitoEAGLE 2016 Verona IT]]<br />
::::* [[MitoFit Science Camp 2016 Kuehtai AT]]<br />
::::* [[MiPNet21.15 IOC112 Kuehtai AT|IOC112 Kuehtai AT]]<br />
::::* [[MiPNet21.04 IOC110 Melbourne AU|IOC110 Melbourne AU]]<br />
::::* [[Research to Practice 2016 Melbourne AU]]<br />
::::* [[Bioblast 2012 | Bioblast 2012 Innsbruck AT]]<br />
::::* [[MiPNet20.10 IOC106 Schroecken|IOC106 Schroecken AT]]<br />
::::* [[MiPNet20.05 IOC104 Greenville |IOC104 Greenville NC US]]<br />
::::* [[IOC100 | IOC100 Schroecken AT]]<br />
::::* [[IOC68 | IOC68 Schroecken AT]]<br />
::::* [[IOC65 | IOC65 Schroecken AT]]<br />
::::* [[MiPNet14.04 IOC51 | IOC51 Schroecken AT]]<br />
::::* [[MiP2015 | MiP2015 Pec pod Snezkou CZ]]<br />
::::* [[MiPschool Greenville 2015 | MiPschool 2015 Greenville NC US]]<br />
<br />
{{Labelingperson<br />
|field of research=Basic<br />
|topics=[[High-resolution respirometry]], [[Mitochondria]], [[Melatonin]], [[Reactive oxygen species]], [[Reactive nitrogen species]], [[Sepsis]]<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit&diff=231737Torres-Quesada 2022 MitoFit2022-08-30T09:56:40Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines. https://doi.org/10.26124/mitofit:2022-0008 - ''2022-08-13 published in https://doi.org/10.3390/cancers14163917''<br />
|info=MitoFit Preprints 2022.8. [[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines]<br/><br />
|authors=Torres-Quesada Omar, Doerrier Carolina, Strich Sophie, Gnaiger Erich, Stefan Eduard<br />
|year=2022-04-01<br />
|journal=MitoFit Prep<br />
|abstract=Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. The simplicity of these models facilitates the reproducible manipulation of cultured cells. Limitations, however, relate to cell growth in a monolayer using standard cell culture media. Although such media are in use for decades, their formulations do not mimic the composition of the originating human cell environment in physiological or pathological conditions. In this study, we selected three frequently-used cancer cell lines SW620 (colon cancer), MCF7 (breast cancer), and A375 (melanoma) to determine the impact of a newly formulated human plasma-like medium (HPLM) on cell proliferation and mitochondrial function. Mitochondrial bioenergetic profiles were analyzed by high-resolution respirometry, revealing metabolic reprogramming in cells cultured in standard and HPLM media. Notably, the culturing of differentially proliferating cells in HPLM affected key mechanism of cellular respiration. All three cancer cell lines displayed an amending bioenergetic profile, particularly related to mitochondrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinically prescribed cancer drug and kinase inhibitor sunitinib. These findings highlight that cell culture media redirect mitochondrial function and affect cancer drug sensitivities. To advance the translational potential of cell culture models we propose to prioritize media with a human plasma-like composition for analyzing bioenergetic profiles and for determining target-oriented drug efficacies. <br><br><br />
|keywords=Cancer cells, cell respiration, mitochondrial function, HPLM, classical media, metabolism, cell culture media, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residual O2 consumption, bioenergetic cluster analysis BCA<br />
|editor=Tindle-Solomon L<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
__TOC__<br />
== Data availability ==<br />
:::: Original files are available Open Access at Zenodo repository: https://doi.org/10.5281/zenodo.6402435<br />
<br />
== Support ==<br />
:::: This work was founded by the projects 877163 for the Austrian FFG-Bridge program [[MitoKIN]], the Austrian Science Fund (FWF; P27606, P30441, P32960, P35159) and the Tyrolean Cancer Society.<br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, ROUTINE, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA, MitoKIN<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_Cancers_(Basel)&diff=231735Torres-Quesada 2022 Cancers (Basel)2022-08-30T09:19:14Z<p>Doerrier Carolina: </p>
<hr />
<div>{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Physiological cell culture media tune mitochondrial bioenergetics and drug sensitivity in cancer cell models. https://doi.org/10.3390/cancers14163917<br />
|info=Cancers (Basel) 14:3917. [https://pubmed.ncbi.nlm.nih.gov/36010911 PMID: 36010911 Open Access]<br />
|authors=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E<br />
|year=2022<br />
|journal=Cancers (Basel)<br />
|abstract=Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. However, many limitations apply to the cell growth in a monolayer using standard cell culture media. Although they have been used for decades, their formulations do not mimic the composition of the human cell environment. In this study, we analyzed the impact of a newly formulated human plasma-like media (HPLM) on cell proliferation, mitochondrial bioenergetics, and alterations of drug efficacies using three distinct cancer cell lines. Using high-resolution respirometry, we observed that cells grown in HPLM displayed significantly altered mitochondrial bioenergetic profiles, particularly related to mitochondrial density and mild uncoupling of respiration. Furthermore, in contrast to standard media, the growth of cells in HPLM unveiled mitochondrial dysfunction upon exposure to the FDA-approved kinase inhibitor sunitinib. This seemingly context-dependent side effect of this drug highlights that the selection of the cell culture medium influences the assessment of cancer drug sensitivities. Thus, we suggest to prioritize media with a more physiological composition for analyzing bioenergetic profiles and to take it into account for assigning drug efficacies in the cell culture model of choice.<br />
|keywords=cancer cells, cell bioenergetics, cell culture media, cell proliferation, kinase inhibitor, mitochondrial function<br />
|editor=Doerrier C<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
== Preprint ==<br />
<br />
::::* [[Torres-Quesada 2022 MitoFit]]<br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, ROUTINE, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA, MitoKIN, cell culture media, kinase inhibitor, sunitinib<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_Cancers_(Basel)&diff=231734Torres-Quesada 2022 Cancers (Basel)2022-08-30T09:17:52Z<p>Doerrier Carolina: Created page with "{{Publication |title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Physiological cell culture media tune mitochondrial bioenergetics and drug sensitivity..."</p>
<hr />
<div>{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Physiological cell culture media tune mitochondrial bioenergetics and drug sensitivity in cancer cell models. https://doi.org/10.3390/cancers14163917<br />
|info=Cancers (Basel) 14:3917. [https://pubmed.ncbi.nlm.nih.gov/36010911 PMID: 36010911 Open Access]<br />
|authors=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E<br />
|year=2022<br />
|journal=Cancers (Basel)<br />
|abstract=Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. However, many limitations apply to the cell growth in a monolayer using standard cell culture media. Although they have been used for decades, their formulations do not mimic the composition of the human cell environment. In this study, we analyzed the impact of a newly formulated human plasma-like media (HPLM) on cell proliferation, mitochondrial bioenergetics, and alterations of drug efficacies using three distinct cancer cell lines. Using high-resolution respirometry, we observed that cells grown in HPLM displayed significantly altered mitochondrial bioenergetic profiles, particularly related to mitochondrial density and mild uncoupling of respiration. Furthermore, in contrast to standard media, the growth of cells in HPLM unveiled mitochondrial dysfunction upon exposure to the FDA-approved kinase inhibitor sunitinib. This seemingly context-dependent side effect of this drug highlights that the selection of the cell culture medium influences the assessment of cancer drug sensitivities. Thus, we suggest to prioritize media with a more physiological composition for analyzing bioenergetic profiles and to take it into account for assigning drug efficacies in the cell culture model of choice.<br />
|keywords=cancer cells, cell bioenergetics, cell culture media, cell proliferation, kinase inhibitor, mitochondrial function<br />
|editor=Doerrier C<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, ROUTINE, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA, MitoKIN, cell culture media, kinase inhibitor, sunitinib<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Doerrier_Carolina&diff=229985Doerrier Carolina2022-07-08T13:52:43Z<p>Doerrier Carolina: </p>
<hr />
<div>{{EAGLE<br />
|COST= Member<br />
|COST WG1 = WG1<br />
|COST WG2 = WG2<br />
|COST WG3 = WG3<br />
|COST WG4 = WG4<br />
|COST ECI= ECI<br />
}}<br />
: [[Management_Committee_MitoEAGLE#MC_Substitutes|MC Substitute]] - [[Management Committee MitoEAGLE]]<br />
{{NextGen-O2k H2020-support}}<br />
{{Person<br />
|lastname=Doerrier Velasco<br />
|firstname=Carolina<br />
|title=PhD.<br />
|institution=::::::::::::::::[[File:DoerrierC.JPG|right|150px|Carolina Doerrier]]<br />
<br />
<br />
<br />
<br />
Background: [[ES Granada Acuna-Castroviejo D]]<br />
<br />
Carolina was part of the [[Oroboros_Contact |Oroboros Team]] from October 2014 to May 2022.<br />
|address=Schoepfstrasse 18<br />
|area code=6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress=carolina.doerrier@gmail.com<br />
|weblink=[http://www.oroboros.at www.oroboros.at], [[Bioblast|www.bioblast.at]], [http://www.mitophysiology.org www.mitophysiology.org], [https://www.researchgate.net/profile/Carolina_Doerrier_Velasco ResearchGate profile], ORCID:[[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]]<br />
}}<br />
<br />
__TOC__<br />
== Bioenergetics Communications ==<br />
::::* Carolina Doerrier is Section Editor of the journal '''[[Bioenergetics Communications]]'''<br />
:::::'''Keywords:''' O2k-FluoRespirometry, oxidative stress, permeabilized muscle fibers, NAD(P)H, sepsis, aging, melatonin<br />
==[[Oroboros Contact| Oroboros-team]]==<br />
:* Previous laboratory: [[ES Granada Acuna-Castroviejo D]], [[AT Innsbruck Oroboros]].<br />
:* Collaboration with the [[AT_Innsbruck_Gnaiger E|Gnaiger Lab]] in Innsbruck during a 3-months scholarship (Sep - Dec 2011), in the frame of the K-Regio project ''[[MitoCom_O2k-Fluorometer| MitoCom Tyrol]]''.<br />
:* Collaboration with the [[US FL Orlando Goodpaster BH|Goodpaster Lab]] in Orlando (2017).<br />
:* MitoEAGLE Short-Term Scientific Mission at [[ES Barcelona Garcia-Roves PM]] in Barcelona (2018).<br />
:* Guest tutor at [[IOC65]] and [[IOC68]]. <br />
:* Tutor at [[IOC100]], [[IOC104]], [[IOC106]], [[IOC110]], [[IOC112]], [[IOC114]], [[IOC115]], [[IOC116]], [[IOC120]], [[IOC122]], [[IOC130]], [[IOC132]], [[IOC133]], [[IOC137]], [[IOC139]], [[IOC144]] and [[IOC145]].<br />
<br />
== Bioblast Editorial Board ==<br />
<br />
:* Bioblast national editor [[MiPNet Laboratories Spain]]<br />
:* [[Library of protocols]]<br />
<br />
== MitoEAGLE Short-Term Scientific Mission ==<br />
****: [[Short-Term Scientific Missions MitoEAGLE#STSM_Grant_Period_2 |STSM Grant Period 2]]<br />
::: '''Work Plan summary'''<br />
:::: WORKPLAN SUMMARY Different experimental aspects (e.g. purity of the preparations, environmental humidity which could affect the wet weight of the muscle, chemicals) will be evaluated during the STSM in order to clarify the sources of variability found in the MitoEAGLE WG2 pilot study. The preparation of the samples will be performed according the SOP established for the MitoEAGLE WG2 pilot study. Permeabilized fibers from soleus of C57BL6/J mice will be used. Mitochondrial respiration will be assessed by O2k high-resolution respirometry (HRR). The Substrate- Uncoupler-Inhibitor Titration (SUIT) protocol used in the MitoEAGLE WG2 pilot study for obtaining mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers, will be used to perform the experiments during the STSM at the Department of Physiological Sciences II in the Faculty of Medicine (Barcelona University, Barcelona, Spain).<br />
<br />
<br />
<br />
== Participated at ==<br />
::::* [[MiPNet 26.16 NextGen-O2k Summit 2021 Virtual]]<br />
::::* [[2020 PaduaMuscleDays Padua IT]]<br />
::::* [[FAT4BRAIN School IOC147 Virtual Event]]<br />
::::* [[MiPNet25.06 IOC145 Innsbruck AT|IOC145 Innsbruck AT]]<br />
::::* [[MiPNet25.03 IOC144 Innsbruck AT|IOC144 Innsbruck AT]]<br />
::::* [[MiP2019/MitoEAGLE Belgrade RS|MitoEAGLE 2019 Belgrade RS]]<br />
::::* [[MiP2019/MitoEAGLE Belgrade RS]]<br />
::::* [[FEBS Workshop Ageing 2019 Innsbruck AT]]<br />
::::* [[Mitochondrial Physiology ‐ from Organelle to Organism 2019 Copenhagen DK]]<br />
::::* [[MiPschool Coimbra 2019|MiP/MitoEAGLE Training School 2019 Coimbra PT]]<br />
::::* [[IOC139| IOC139 Schroecken AT]]<br />
::::* [[Life Science PhD Meeting 2019 Innsbruck AT]]<br />
::::* [[MitoEAGLE Obergurgl 2019-01-31| MitoEAGLE 2019 Obergurgl AT]]<br />
::::* [[IOC137|IOC137 Innsbruck AT]]<br />
::::* [[MitoEAGLE_Innsbruck_2018-11-19| MitoEAGLE 2018 Innsbruck AT]]<br />
::::* [[IOC133|IOC133 Innsbruck AT]]<br />
::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]<br />
::::* [[EBEC2018 Budapest HU]]<br />
::::* [[MitoEAGLE Copenhagen 2018| MitoEAGLE 2018 Copenhagen DK]]<br />
::::* [[MiPNet23.06 IOC130 Schroecken AT| IOC130 Schroecken AT]]<br />
::::* [[ESCI 2018 Barcelona ES]]<br />
::::* [[MitoFit Workshop ATP 2017 Innsbruck AT]]<br />
::::* [[MiP2017/MitoEAGLE Hradec Kralove CZ|MitoEAGLE 2017 Hradec Kralove CZ]]<br />
::::* [[MiPNet22.01 IOC122 Schroecken AT]]<br />
::::* [[MitoEAGLE Obergurgl 2017| MitoEAGLE 2017 Obergurgl AT]]<br />
::::* [[MiPschool Obergurgl 2017| MiPschool 2017 Obergurgl AT]]<br />
::::* [[MitoEAGLE Barcelona 2017| MitoEAGLE 2017 Barcelona ES]]<br />
::::* [[MiPNet22.04 IOC120 Barcelona ES | IOC120 Barcelona ES]]<br />
::::* [[Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE| Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE]]<br />
::::* [[MiPNet21.11 IOC116 Innsbruck AT|IOC116 Innsbruck AT]]<br />
::::* [[IOC115 | IOC115 Schroecken AT]]<br />
::::* [[MiPNet21.19 IOC114 Innsbruck AT | IOC114 Innsbruck AT]]<br />
::::* [[MitoEAGLE Verona 2016| MitoEAGLE 2016 Verona IT]]<br />
::::* [[MitoFit Science Camp 2016 Kuehtai AT]]<br />
::::* [[MiPNet21.15 IOC112 Kuehtai AT|IOC112 Kuehtai AT]]<br />
::::* [[MiPNet21.04 IOC110 Melbourne AU|IOC110 Melbourne AU]]<br />
::::* [[Research to Practice 2016 Melbourne AU]]<br />
::::* [[Bioblast 2012 | Bioblast 2012 Innsbruck AT]]<br />
::::* [[MiPNet20.10 IOC106 Schroecken|IOC106 Schroecken AT]]<br />
::::* [[MiPNet20.05 IOC104 Greenville |IOC104 Greenville NC US]]<br />
::::* [[IOC100 | IOC100 Schroecken AT]]<br />
::::* [[IOC68 | IOC68 Schroecken AT]]<br />
::::* [[IOC65 | IOC65 Schroecken AT]]<br />
::::* [[MiPNet14.04 IOC51 | IOC51 Schroecken AT]]<br />
::::* [[MiP2015 | MiP2015 Pec pod Snezkou CZ]]<br />
::::* [[MiPschool Greenville 2015 | MiPschool 2015 Greenville NC US]]<br />
<br />
{{Labelingperson<br />
|field of research=Basic<br />
|topics=[[High-resolution respirometry]], [[Mitochondria]], [[Melatonin]], [[Reactive oxygen species]], [[Reactive nitrogen species]], [[Sepsis]]<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Bioblast_2022&diff=227283Bioblast 20222022-05-17T18:27:25Z<p>Doerrier Carolina: </p>
<hr />
<div>[[File:Bioblast2022 banner.jpg]]<br />
{{Template:Oroboros_highlights}}<br />
<br />
{{Publication<br />
|title=[[File:BEC.png|25px|link=https://www.bioenergetics-communications.org/index.php/bec/Bioblast2022]] '''Innsbruck, AT''', 2022 Jun 29 - 30. '''Bioblast 2022: BEC Inaugural Conference'''<br />
|info=<br />
|authors= Oroboros Instruments<br />
|year=2022-06-29<br />
|journal=MitoGlobal<br />
|abstract= '''Bioblast 2022: Inaugural Conference of ''Bioenergetics Communications'''''}}<br />
* Celebrating 30 years Oroboros - a follow-up of '''[[Bioblast 2012]]'''<br />
[[File:BEC-exlibris.png|right|290px|Bioenergetics Communications|link=https://www.bioenergetics-communications.org/index.php/bec/index]]<br />
__TOC__<br />
<br />
== Venue ==<br />
:::: [https://www.cmi.at/de/home.html Congress Innsbruck] - Saal Brussels (280 seats)<br />
:::: Rennweg 3<br />
:::: 6020 Innsbruck, AT<br />
<br />
:::: [[File:CongressInnsbruck.jpg|450px|link=http://www.congress-innsbruck.at/|venue]] [[File:Congress-daytime.jpg|385px|link=http://www.congress-innsbruck.at/|venue]]<br />
<br />
<br />
== Registration ==<br />
<br />
:::: Register now [https://www.oroboros.at/index.php/bioblast-2022-registration/ here]<br />
<br />
:::: Early Registration fee until May 15<sup>th</sup><br />
== Poster and oral presentation guidelines ==<br />
::: '''Oral presentations''' are mainly reserved for manuscript contributions to the [https://www.bioenergetics-communications.org/index.php/bec/Bioblast2022 BEC Inaugural Issue].<br />
<br />
:::: Presenters of BEC contributions are invited speakers. Please register, but your registration fee is waived.<br />
<br />
::: Abstracts without manuscripts will be selected for poster presentations. Additional oral presentations may be available (10 min talk plus questions or 5 min talk).<br />
<br />
::: For '''poster presentations''', please send a title, abstract and presentation preference (poster only or poster with talk) by '''May 15''' to [mailto:beceditor@bioenerg-commun.org beceditor@bioenerg-commun.org] and fill out the [https://www.oroboros.at/index.php/bioblast-2022-registration/ registration form].<br />
<br />
::::* [[Abstract format]]<br />
<br />
::::* Submitted abstracts will be published on the Bioblast website.<br />
::: '''Full Manuscript submissions for BEC Inaugural Issue to be submitted in time for 2022 publication'''<br />
::::* Abstracts for presentations to be submitted as indicated above.<br />
::::* [https://www.bioenergetics-communications.org/index.php/bec/Bioblast2022 More information]<br />
== Program ==<br />
[[File:Bioblast-icon.jpg|right|250px|Artist: Bernd Weinmayer; Photo: Philipp Gradl]]<br />
::: '''Topics and >25 suggested titles'''<br />
<br />
::::# '''Evolutionary perspectives of bioenergetics'''<br />
::::## [[Lane Nick |The alpha and omega of metabolism: why the Krebs cycle brings the earth to life and our own lives to an end]]'''**'''<br />
::::## Turning points in bioenergetics - evolutionary perspectives on rotation gear ratios of F<sub>1</sub>F<sub>O</sub>-ATPases<br />
::::## [[Bioblasts |Bioblasts - the taxonomic unit of bioenergetics: mitochondria, chloroplasts, aerobic bacteria''']]'''**'''<br />
::::## [[Gnaiger 1977 Invertebrate anoxibiosis |Reverse citric acid cycle and mitochondrial succinate formation under anoxia: from parasites and invertebrates to ischemia-reperfusion]]'''**'''<br />
::::## Symmorphosis and the respiratory cascade - does mitochondrial physiology match?<br />
::::# '''Algal bioenergetics'''<br />
::::## [[ALAS |Reactive carbonyls contribute to photo-oxidative stress acclimation]]'''**'''<br />
::::## [[Morosinotto Tomas |Photosynthetic capacity under oscillating light regimes]]'''**'''<br />
::::# '''Molecular, kinetic, and thermodynamic advances in bioenergetics'''<br />
::::## Signals of oxygen to mitochondria<br />
::::## Cellular oxygen kinetics with and beyond Complex IV<br />
::::## [[HU Budapest Tretter L |The protonmotive force - not merely membrane potential]]'''**'''<br />
::::# '''From bioenergetics to comparative mitochondrial physiology'''<br />
::::## [[MitoEAGLE 2022 MitoFit Hypoxia |The ABC of hypoxia – what is the norm?]]'''**'''<br />
::::## Climate change and mitochondrial physiology<br />
::::## Hibernating mitochondria<br />
::::## Metabolic shutdown of mitochondrial bioenergetics<br />
::::# '''Perspectives of bioenergetics in health and disease'''<br />
<br />
::::## [[Sobotka Lubos |The Crabtree effect and clinical nutrition]]'''**'''<br />
::::## [[MX Mexiko City Moreno-Sanchez R |Estimation of energy pathway fluxes in cancer cells- beyond the Warburg effect]]'''**'''<br />
::::## [[Weiss Guenter |Linkage of iron availability to mitochondrial function]]'''**'''<br />
::::## [[AT Innsbruck Schneeberger S |Pre-transplant mitochondrial respiration as a clinical prognostic marker in liver machine perfusion]]'''**'''<br />
::::## [[Chakrabarti Sasanka |Rotenone cytotoxicity on SH-SY5Y cells: role of ferroptosis, mitochondria and alpha-synuclein]]'''**'''<br />
::::## [[Karabatsiakis Alexander |Effects of eye-movement desensitization and reprocessing (EMDR) therapy on mitochondrial bioenergetics in immune cells from patients with post-traumatic stress disorder (PTSD): a pilot study]]'''**'''<br />
::::## [[Iyer Shilpa|Bioenergetics Health Index Ratio in Leigh Syndrome patient fibroblasts as a measure of disease severity]]'''**'''<br />
::::## [[Stiban Johnny|NUBPL: a mitochondrial Complex I deficiency disorder ]]'''**'''<br />
::::## [[Mutschler R |Membranes as therapeutic targets - liposomes as therapeutic options.]]'''**'''<br />
::::## [[Molina Anthony JA|Platelet bioenergetics are associated with resting metabolic rate and exercise capacity in older adult women]]'''**'''<br />
::::## [[Neuzil Jiri|Metabolic switch utilizing ammonia supports proliferation in regenerating liver]]'''**'''<br />
::::## Mitochondrial bioenergetics in ischemia-reperfusion injury<br />
::::## Mitochondrial research at high altitude - a respiratory challenge<br />
::::## Sex and mitochondrial function in high-performance sports<br />
::::## Long COVID - a respiratory mitochondrial issue<br />
::::# '''Mitochondrial pharmacology'''<br />
::::## [[Koopman_Werner_JH |The decylTPP mitochondria-targeting moiety lowers electron transport system supercomplex levels in primary human skin fibroblasts]]'''**'''<br />
::::## [https://abliva.com/company/ Towards a treatment for mitochondrial disease: Current compounds in clinical development]'''**'''<br />
::::## [[MitoKIN |Kinase perturbations redirect mitochondrial function]]'''**'''<br />
::::# '''Methodological advancements in bioenergetics'''<br />
::::## [[Ecker Rupert|Contextual Tissue Cytometry with AI – Functional Single Cell Analyses ''in-situ'']]'''**'''<br />
::::## [[Cardoso 2022 Abstract Bioblast |Redox monitoring and respiration - a new horizon with the NextGen-O2k]]'''**'''<br />
::::## [[Garcia-Roves Pablo Miguel | Tuning the assessment of coenzyme Q redox state and respiration in skeletal muscle permeabilized fibers]]'''**'''<br />
::::## [[TissueModels |Lipidomics of mitochondrial membranes - methods first]]'''**'''<br />
::::## [https://www.incyton.com/ Physiometabolic RTCA on an automated platform for short and long term applications]'''**'''<br />
::::## [[Chicco Adam J |Resolving the Rotenone Paradox: elucidating the Complexity of multi-substrate respirometry protocols]]'''**'''<br />
::::## [[Baglivo Eleonora|Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry]]'''**'''<br />
::::## [[Posch 2022 MitoFit |How to optimize respiratory models for SARS-CoV-2 research]]'''**'''<br />
::::## [[Cecatto Cristiane|Mitochondrial calcium uptake capacity is lower than calcium retention capacity in the presence and absence of cyclosporin A]] '''**'''<br />
::::## [[Chinopoulos Christos|An NADH-dependent link between safranin oxidoreduction and succinate dehydrogenase reversal during anoxia]] '''**'''<br />
::::## Culture media and cell bioenergetics - beyond high and low glucose<br />
::::# '''Round table: Oroboros Diagnostics'''<br />
:::::: '''**''' ''confirmed''<br />
<br />
<br />
<br />
::: '''Time lines'''<br />
:::: '''2022-06-28''' Arrival in Innsbruck<br />
<br />
:::: '''2022-06-29''' Day 1 of scientific presentations<br />
::::::: - 08:30-09:15 - Registration <br />
::::::: - 09:15-11:00 - Scientific Session I<br />
::::::: - 11:00-11:30 - Coffee break<br />
::::::: - 11:30-12:30 - Scientific Session II<br />
::::::: - 12:30-14:00 - Lunch break <br />
::::::: - 14:00-15:30 - Scientific Session III<br />
::::::: - 15:30-16:00 - Coffee break<br />
::::::: - 16:00-17:15- Scientific Session IV<br />
::::::: - 18:30 - Evening social activity: Take the Hungerburg funicular and 45 minute hike to Umbrüggler Alm - Dinner <br />
<br />
:::: '''2022-06-30''' Day 2 of scientific presentations<br />
::::::: - 09:15-10:30 - Scientific Session I<br />
::::::: - 10:30-11:00 - Coffee break<br />
::::::: - 11:00-12:30 - Scientific Session II<br />
::::::: - 12:30-14:00 - Light lunch<br />
::::::: - 14:00-15:30 - Scientific Session III<br />
::::::: - 15:30-16:00 - Coffee break<br />
::::::: - 16:00-17:30 - Scientific Session IV<br />
::::::: - 18:00-19:00 Social program: Guide tour through Hofkirche<br />
::::::: - 19:00- open end: Celebration Dinner: 30 years Oroboros at Brahms Innsbruck <br />
<br />
:::: '''2022-07-01''' Departure from Innsbruck<br />
<br />
<br />
== Speakers - preliminary list==<br />
<gallery mode=default perrow=8 widths="140px" heights="150px"><br />
<br />
File:Eleonor A Frostner.jpg|'''[[Aasander Frostner Eleonor]]''' <br>(Abliva AB and Lund University, SE)<br />
<br />
File:BaglivoE.jpg|'''[[Baglivo Eleonora]]''' (Oroboros Instruments, AT)<br />
File:CardosoLHD.JPG|'''[[Cardoso Luiza HD]]''' (Oroboros Instruments, AT)<br />
<br />
File:Cristiane Cecatto.jpg|'''[[Cecatto Cristiane]]''' (Oroboros Instruments, AT)<br />
<br />
File:Chakrabarti S.JPG|'''[[Chakrabarti Sasanka]]''' (Maharishi Markandeshwar Deemed University (MMDU), IN)<br />
File:Chicco Headshot.jpg|'''[[Chicco Adam J]]''' <br>(Colorado State University, US)<br />
File:Donnellyc.jpg|'''[[Donnelly Chris]]''' <br>(Institute of Sport Sciences of the University of Lausanne (ISSUL), CH)<br />
<br />
File:Ecker Rupert.jpg|'''[https://tissuegnostics.com/ Ecker Rupert]''' <br>(TissueGnostics, AT and Queensland University of Technology, AU)<br />
<br />
File:Garcia-RovesP.jpg|'''[[Garcia-Roves Pablo M]]''' (University of Barcelona and Bellvitge Biomedical Research Institute (IDIBELL), ES)<br />
File:Erich Gnaiger.jpg|'''[[Gnaiger Erich]]''' <br>(Oroboros Instruments, AT)<br />
<br />
<br />
File:Irving Brian.jpg|'''[[Irving Brian A]]''' <br>(Louisiana State University, US)<br />
<br />
File:Alexander Karabatsiakis.jpg|'''[[Karabatsiakis Alexander]]''' <br>(University of Innsbruck, AT)<br />
<br />
File:KomlodiT.JPG|'''[[Komlodi Timea]]''' <br>(Semmelweis University, HU)<br />
File:Koopman WJH.JPG|'''[[Koopman Werner JH]]''' <br>(Radboud University Nijmegen Medical Centre, NL)<br />
File:MeszarosA.JPG|'''[[Meszaros Andras]]''' <br>(Medical University of Innsbruck, AT)<br />
<br />
File:Molina-Anthony-J.jpg|'''[[Molina Anthony JA]]''' <br>(University of California San Diego, US )<br />
<br />
File:Profile-icon-9.png|'''[[Moreno-Sanchez Rafael]]''' <br>(National Institute of Cardiology, MX)<br />
<br />
File:Morosinotto T.jpg|'''[[Morosinotto Tomas]]''' <br>(Università di Padova, IT) <br />
<br />
File:Dr.Rainer Mutschler.png|'''[[Mutschler R|Mutschler Rainer]]''' <br>(BioMedical Center Speyer , DE)<br />
File:Neuzil J.png|'''[[Neuzil Jiri]]''' <br>(Czech Academy of Sciences, CZ and Griffith University, AU)<br />
<br />
File:Roach T.jpg|'''[[Roach Thomas]]''' <br>(University of Innsbruck, AT)<br />
<br />
File:Profile-icon-9.png|'''[[Sobotka Lubos]]''' <br>(Charles University, CZ)<br />
File:Profile-icon-9.png|'''[[Stefan Eduard]]''' <br>(University of Innsbruck, AT)<br />
File:Stiban J.jpg|'''[[Stiban Johnny]]''' <br>(Birzeit University, PS)<br />
File:Torres-QuesadaO.jpg|'''[[Torres-Quesada Omar]]''' <br>(University of Innsbruck, AT)<br />
File:Tretter L.JPG|'''[[Tretter Laszlo]]''' <br>(Semmelweis University, HU)<br />
<br />
<br />
File:Wilflingseder D.jpg|'''[[Wilflingseder Doris]]''' <br>(Medical University of Innsbruck, AT)<br />
<br />
File:Dr. Peter Wolf.jpg|'''[[Wolf Peter]]''' <br>(Incyton, DE)<br />
<br />
|-<br />
<br />
</gallery><br />
== Industrial and science exhibition ==<br />
<br />
<br />
[[Image:Incyton Logo 30 mm original.jpg|250 px|link=https://www.incyton.com/]]<br />
[[Image:WGT Logo 2012.jpg|250 px|link=https://www.wgt.at/]]<br />
[[Image:Oro logo.jpg|130 px|link=https://www.oroboros.at/]]<br />
[[Image:MiPsocietyLOGO.JPG|250px|link=https://www.mitophysiology.org|MiP''society'']]<br />
[[Image:TissueGnostics Logo.png|200px|link=https://www.tissuegnostics.com/]]<br />
[[Image:Tirol sat stacked RGB.png|200 px|link=https://www.standort-tirol.at/]]<br />
<br />
<br />
== Contributions==<br />
<br />
=== Submitted manuscripts ===<br />
:::: Under review for acceptance<br />
{{#ask:[[Additional label::Bioblast 2022]]<br />
| mainlabel<br />
|?Has title=Reference<br />
|?Was published in year=Year<br />
|?Has info=View<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Has title<br />
|order=ascending<br />
}}<br />
<br />
=== Submitted abstracts ===<br />
:::: Under review for acceptance<br />
{{#ask:[[was submitted to event::Bioblast_2022]]<br />
| ?has title=Reference<br />
| limit=100<br />
<br />
|format=table<br />
|sort=<br />
|order=ascending<br />
|default=no abstracts found<br /><br /><br />
}}<br />
== International scientific committee (preliminary) ==<br />
<br />
::::* [[Aasander Frostner Eleonor]], SE '''**'''<br />
::::* [[Chakrabarti Sasanka]], IN<br />
::::* [[Chicco Adam J]], US '''**'''<br />
::::* [[Donnelly Chris]], CH '''**'''<br />
::::* [[Ecker Rupert]], AT and AU<br />
::::* [[Garcia-Roves Pablo M]], ES '''**'''<br />
::::* [[Hoppel Charles L]], US '''**'''<br />
::::* [[Irving Brian A]], US '''**'''<br />
::::* [[Karabatsiakis Alexander]], AT<br />
::::* [[Keller Markus A]], AT<br />
::::* [[Komlodi Timea]], HU<br />
::::* [[Koopman Werner JH]], NL<br />
::::* [[Lane Nick]]<br />
::::* [[Meszaros Andras]], AT<br />
::::* [[Molina Anthony JA]], US '''**'''<br />
::::* [[Moore Anthony L]], UK<br />
::::* [[Moreno-Sanchez Rafael]], MX<br />
::::* [[Morosinotto Tomas]], IT '''**'''<br />
::::* [[Mutschler Rainer]], DE<br />
::::* [[Oliveira Marcus F]], BR<br />
::::* [[Roach Thomas]], AT '''**'''<br />
::::* [[Sazanov Leonid A]], AT '''**'''<br />
::::* [[Sobotka Lubos]], CZ<br />
::::* [[Stefan Eduard]], AT '''**'''<br />
::::* [[Stiban Johnny]], PS '''**'''<br />
::::* [[Strehle Martin]], DE '''**'''<br />
::::* [[Torres-Quesada Omar]], AT '''**'''<br />
::::* [[Tretter Laszlo]], HU<br />
::::* [[Weiss Guenter]], AT<br />
::::* [[Wilflingseder Doris]], AT<br />
::::* [[Wolf Peter]], DE<br />
:::::: '''**''' ''confirmed''<br />
== Travel ==<br />
<br />
:::: [[File:Innsbruck travel.png|600px|Travel Innsbruck]]<br />
<br />
== Social programm ==<br />
:::: Further information will follow.<br />
:::: [[File:Black-knights.jpg|400px|Black knights]]<br />
<br />
<br />
<br />
== Bioblast 2022 and EBEC 2024 announcements ==<br />
:::: Follow the latest announcements about Bioblast 2022 on [https://www.linkedin.com/company/oroboros-instruments-gmbh/?viewAsMember=true LinkedIn]<br />
<br />
:::: If you would like to receive announcements to your Inbox, please subscribe to Oroboros emails by sending us a message: [mailto:instruments@oroboros.at instruments@oroboros.at]<br />
<br />
:::: Or you can read them here: [[Bioblast 2022: Announcements]]<br />
<br />
[[File:EBEC22 see-you-in-Innsbruck.png|800px|EBEC2024 see you in Innsbruck|link=EBEC2024 Innsbruck AT]]<br />
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]] <br />
Listed under [[MitoGlobal Events]].<br />
<br />
{{Labeling<br />
|instruments=<br />
|additional=2022, MitoGlobal, ORO, Next<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=MiPNet26.12_NextGen-O2k:_NADH-Module&diff=227234MiPNet26.12 NextGen-O2k: NADH-Module2022-05-17T15:05:24Z<p>Doerrier Carolina: </p>
<hr />
<div>{{OROBOROS header page name}}<br />
{{Publication<br />
|title=[[Image:O2k-Manual.jpg|right|70px|link=O2k-Manual|O2k-Manual]] NextGen-O2k: NADH-Module manual<br />
|info=[[File:PDF.jpg|100px|link=https://wiki.oroboros.at/images/b/bc/MiPNet26.12_NextGen-O2k_NADH-Module.pdf]] » [https://www.bioblast.at/index.php/File:MiPNet26.12_NextGen-O2k_NADH-Module.pdf Versions]<br />
|authors=Oroboros<br />
|year=2022-05-17<br />
|journal=Mitochondr Physiol Network<br />
|abstract=Doerrier C, Schwaninger H, Walter-Vracevic M, Haider M, Niedenzu W, Komlódi T, Gradl P, Gnaiger E (2022) Mitochondr Physiol Network 26.12(01):1-12.<br />
|mipnetlab=AT_Innsbruck_Oroboros<br />
}}<br />
{{MiPNet pdf page linking to MitoPedia NextGen-O2k and DatLab 8}}<br />
<br />
'''Acknowledgements'''<br />
[[File:Template NextGen-O2k.jpg|left|400px|link=NextGen-O2k]]<br />
<br/><br />
<br/><br />
<br/><br />
<br/><br />
<br/><br />
== Cited by ==<br />
<br />
<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|instruments=Oxygraph-2k, NextGen-O2k, O2k-Manual<br />
|additional=O2k-Demo, O2k-MultiSensor, MitoFitPublication, O2k-Protocol, MitoFit 2022 NADH, NextGen-O2k-DL8, DL8<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=File:MiPNet26.12_NextGen-O2k_NADH-Module.pdf&diff=227233File:MiPNet26.12 NextGen-O2k NADH-Module.pdf2022-05-17T15:03:37Z<p>Doerrier Carolina: </p>
<hr />
<div>* '''Current Version 01: 2022-05-17.''' Doerrier C, Schwaninger H, Walter-Vracevic M, Haider M, Niedenzu W, Komlódi T, Gradl P, Gnaiger E (2022) NextGenO2k: NADH-Module manual. Mitochondr Physiol Network 26.12(01):1-12.<br />
* '''Version 00: 2021-10-29.''' Doerrier C, Schwaninger H, Walter-Vracevic M, Haider M, Niedenzu W, Komlódi T, Gradl P, Gnaiger E (2021) NextGenO2k: NADH-Module manual. Mitochondr Physiol Network 26.12(00):1-12.</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=File:MiPNet26.12_NextGen-O2k_NADH-Module.pdf&diff=227232File:MiPNet26.12 NextGen-O2k NADH-Module.pdf2022-05-17T15:02:34Z<p>Doerrier Carolina: Doerrier Carolina uploaded a new version of File:MiPNet26.12 NextGen-O2k NADH-Module.pdf</p>
<hr />
<div>* '''Current Version 00: 2021-10-29.''' Doerrier C, Schwaninger H, Walter-Vracevic M, Haider M, Niedenzu W, Komlódi T, Gradl P, Gnaiger E (2021) NextGenO2k: NADH-Module manual. Mitochondr Physiol Network 26.12(00):1-12.<br />
* '''Current Version 01: 2022-05-17.''' Doerrier C, Schwaninger H, Walter-Vracevic M, Haider M, Niedenzu W, Komlódi T, Gradl P, Gnaiger E (2021) NextGenO2k: NADH-Module manual. Mitochondr Physiol Network 26.12(01):1-12.</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=File:MiPNet26.12_NextGen-O2k_NADH-Module.pdf&diff=227231File:MiPNet26.12 NextGen-O2k NADH-Module.pdf2022-05-17T15:00:48Z<p>Doerrier Carolina: </p>
<hr />
<div>* '''Current Version 00: 2021-10-29.''' Doerrier C, Schwaninger H, Walter-Vracevic M, Haider M, Niedenzu W, Komlódi T, Gradl P, Gnaiger E (2021) NextGenO2k: NADH-Module manual. Mitochondr Physiol Network 26.12(00):1-12.<br />
* '''Current Version 01: 2022-05-17.''' Doerrier C, Schwaninger H, Walter-Vracevic M, Haider M, Niedenzu W, Komlódi T, Gradl P, Gnaiger E (2021) NextGenO2k: NADH-Module manual. Mitochondr Physiol Network 26.12(01):1-12.</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=O2k-Network_discussion_forum&diff=227221O2k-Network discussion forum2022-05-17T14:04:27Z<p>Doerrier Carolina: </p>
<hr />
<div>{{OROBOROS header page name}}<br />
[[File:O2k-Network.png|right|150px|link=O2k-Network|O2k-Network]]<br />
<br />
:::: Comments or questions pertaining to topics addressed at the Bioblast website can be posted on the corresponding '''Discussion page''' or '''Talk''' page.<br />
<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
:::: We encourage you to initiate discussions on specific topics, or post questions and comments. To do so, simply send an Email to the moderator, indicating the topic/keyword and the question. We will then send an Email circular to registered O2k-Network members ([[O2k-Network |O2k-Network Labs]]). In addition, the questions and follow-up correspondence will be posted on the Bioblast website.<br />
<br />
::::» Email to the moderator: [mailto:cristiane.cecatto@oroboros.at| cristiane.cecatto@oroboros.at] <br />
<br />
<br />
== Current topics ==<br />
<br />
<br />
== Previous topics ==<br />
:::# [[Talk:Oligomycin]]<br />
:::# [[Talk:Respirometry]]<br />
:::# [[Talk:Measuring hydrogen peroxide]]<br />
:::# [[Talk:Digitonin]]<br />
:::# [[Talk:Fatty acid oxidation| Talk:Fatty acid oxidation on permeabilized fibres]]<br />
:::# [[Talk:Cytochrome c]]<br />
<br />
<br />
» [[Talk:OROBOROS_MitoFit_Laboratory#Picked_up_-_reading_and_discussion |'''Picked up - reading and discussion''']]</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=O2k-Network_discussion_forum&diff=227220O2k-Network discussion forum2022-05-17T14:04:15Z<p>Doerrier Carolina: </p>
<hr />
<div>{{OROBOROS header page name}}<br />
[[File:O2k-Network.png|right|150px|link=O2k-Network|O2k-Network]]<br />
<br />
:::: Comments or questions pertaining to topics addressed at the Bioblast website can be posted on the corresponding '''Discussion page''' or '''Talk''' page.<br />
<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
:::: We encourage you to initiate discussions on specific topics, or post questions and comments. To do so, simply send an Email to the moderator, indicating the topic/keyword and the question. We will then send an Email circular to registered O2k-Network members ([[O2k-Network |O2k-Network Labs]]). In addition, the questions and follow-up correspondence will be posted on the Bioblast website.<br />
<br />
::::» Email to the moderator: [mailto:cristiane.cecatto@oroboros.at| cristiane.cecatto@oroboros.at] <br />
<br />
<br />
== Current topics ==<br />
:::# [[Talk:Cytochrome c]]<br />
<br />
== Previous topics ==<br />
:::# [[Talk:Oligomycin]]<br />
:::# [[Talk:Respirometry]]<br />
:::# [[Talk:Measuring hydrogen peroxide]]<br />
:::# [[Talk:Digitonin]]<br />
:::# [[Talk:Fatty acid oxidation| Talk:Fatty acid oxidation on permeabilized fibres]]<br />
<br />
<br />
» [[Talk:OROBOROS_MitoFit_Laboratory#Picked_up_-_reading_and_discussion |'''Picked up - reading and discussion''']]</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=NADH-Sensor&diff=227182NADH-Sensor2022-05-16T10:06:33Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=<br />
|description= The '''NADH-Sensor''' has been developed as a part of the [[NADH-Module]] for simultaneous monitoring of oxygen consumption and [[NADH redox state]]. The NADH-Sensor is composed of a photodiode and equipped with three supergel R370 Italian blue filters (Rosco, US). <br />
}}<br />
<br />
== The NADH-Sensor is an integral part of the [[NADH-Module]] and the [[NextGen-O2k]] project==<br />
{{Template:NADH-Module}}<br />
<br />
Communicated by [[Doerrier C]] 2021-10-07<br />
<br />
{{NextGen-O2k}}<br />
<br />
{{Labeling<br />
|additional=MitoPedia:NextGen-O2k<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=FEBS_Workshop_Ageing_2019_Innsbruck_AT&diff=227114FEBS Workshop Ageing 2019 Innsbruck AT2022-05-13T12:42:07Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MiP header page name}}<br />
{{Publication<br />
|title=[[Image:FEBS-logo.png|left|100px|FEBS]] '''Innsbruck, AT'''. 2019 Sep 09-12, FEBS Workshop “Ageing and Regeneration”. <br />
|info= [https://ageingandregeneration2019.febsevents.org FEBS Workshop website]<br />
|authors=Jansen-Duerr P<br />
|year=2019-09-09 <br />
|journal=MitoGlobal<br />
|abstract=<br />
}}<br />
<br />
== Venue ==<br />
:::: Leopold-Franzens-University Innsbruck<br />
:::: Aula, Innrain 52<br />
:::: A-6020 Innsbruck, Austria<br />
<br />
== Oroboros presentation ==<br />
:::: '''[[Doerrier C|Doerrier Carolina]]''': ''Mitochondrial fitness, degenerative diseases and aging.''<br />
<br />
== Preliminary Programme ==<br />
:::: download [http://wiki.oroboros.at/images/4/48/FEBS_workshop_Ageing_and_Regeneration_preliminary_programme_17122018.pdf here]<br />
<br />
== Registration == <br />
:::: There are different rates for senior/corporate scientists and young scientists, either needing accommodation or not and opting for single or double occupancy. For all participants most of the meals will be covered by the registration fee.<br />
<br />
:::: Please visit [https://ageingandregeneration2019.febsevents.org https://ageingandregeneration2019.febsevents.org] to register. <br />
:::: Online registration:February 1 - May 1, 2019.<br />
<br />
== Organizer == <br />
::::* FEBS Member-in-Charge<br />
::::: Winnie ESKILD, Oslo, Norway<br />
::::* Organizing Committee<br />
::::: Frank Edenhofer, Innsbruck, Austria<br />
::::: [[Jansen-Duerr P|Pidder Jansen-Dürr]], Innsbruck, Austria<br />
::::: Ilse Kranner, Innsbruck, Austria<br />
::::: Björn Schumacher, Cologne, Germany<br />
::::: Werner Zwerschke, Innsbruck, Austria<br />
<br />
== Speakers ==<br />
:::: Poster with speaker information available for download [http://wiki.oroboros.at/images/3/34/20181217_FEBS_Poster_sm.pdf here]<br />
<br />
== MitoEAGLE ITC Conference Grant ==<br />
::::* [[MitoEAGLE_Inclusiveness_Target_Countries#Conference_Grant|Eligibility information and user guide]]<br />
<br />
<br />
== Support ==<br />
{{NextGen-O2k H2020-support}}<br />
:::: Oroboros participation was supported by the project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.<br />
<br/><br />
<br/><br />
<br/><br />
<br />
[[File:MitoGlobal.jpg|right|80px|MitoGlobal|link=MitoGlobal]]<br />
<br />
Listed under [[MitoGlobal_Events|MitoGlobal Events]].<br />
{{Labeling<br />
|additional=2019, ORO, MitoGlobal, NextGen-O2k<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Select_O2k_-_DatLab&diff=227059Select O2k - DatLab2022-05-11T08:19:56Z<p>Doerrier Carolina: Created page with "{{Technical support}} {{MitoPedia without banner |description='''Select O2k - DatLab''' |info= }} __TOC__ == DatLab 8 == Under construction {{Labeling |area=Instrument..."</p>
<hr />
<div>{{Technical support}}<br />
{{MitoPedia without banner<br />
|description='''Select O2k - DatLab''' <br />
|info=<br />
}}<br />
<br />
__TOC__<br />
== DatLab 8 ==<br />
<br />
Under construction <br />
<br />
<br />
<br />
{{Labeling<br />
|area=Instruments;methods<br />
|additional=DatLab, DL8, DatLab 8<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=DatLab<br />
}}<br />
[[Category:DatLab 8]]</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=SUIT-006_NADH_mt_D084&diff=226797SUIT-006 NADH mt D0842022-05-03T12:08:04Z<p>Doerrier Carolina: Created page with "{{MitoPedia |abbr= |description=400px |info='''A''': protocol for simultaneous determination of O<sub>2</sub> flux and NADH autoflu..."</p>
<hr />
<div>{{MitoPedia<br />
|abbr=<br />
|description=[[File:1PGM;2D;3U;3anoxia;4Myx;5NADH.png|400px]]<br />
|info='''A''': protocol for simultaneous determination of O<sub>2</sub> flux and NADH autofluorescence in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-006]]'''<br />
|application=NADH<br />
|SUIT number=D084_1PGM;2D;3U;3anoxia;4Myx;5NADH<br />
}}<br />
<br />
<br />
__TOC__<br />
Communicated by [[Doerrier C]] (last update 2022-05-03) <br />
<br />
<br />
== Representative traces ==<br />
<br />
[[File:SUIT-006 NADH mt D084 O2.png|600px]]<br />
[[File:SUIT-006 NADH mt D084.png|600px]]<br />
<br />
{{Template:SUIT-006 NADH mt D084}}<br />
<br />
== Strengths and limitations ==<br />
<br />
<br />
<br />
== Compare SUIT protocols ==<br />
<br />
== Chemicals and syringes ==<br />
::: Suggested stock concentrations are shown in the specific DL-Protocol.<br />
<br />
== References ==<br />
{{#ask:[[Category:Publications]] [[Additional label::SUIT-006 NADH mt D084]] [[Instrument and method::O2k-Protocol]]<br />
| ?Was published in year=Year<br />
| ?Has title=Reference<br />
| ?Mammal and model=Organism<br />
| ?Tissue and cell=Tissue;cell<br />
| format=broadtable<br />
| limit=5000<br />
| offset=0<br />
| sort=Was published in year<br />
| order=descending<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Doerrier_2022_MitoFit_NADH&diff=226783Doerrier 2022 MitoFit NADH2022-05-03T08:06:27Z<p>Doerrier Carolina: </p>
<hr />
<div>{{Publication<br />
|title=Doerrier C, Cardoso LHD, Gnaiger E (2022) Simultaneous measurement of oxygen consumption and NAD(P)H/NAD(P)<sup>+</sup>-redox state in isolated mitochondria. (in prep)<br />
|info=<br />
|authors=<br />
|year=<br />
|journal=<br />
|abstract=<br />
|keywords=NADH fluorescence, NAD-pool, N-junction, NAD-redox state, high-resolution respirometry HRR, isolated mitochondria, mouse liver mitochondria, SUIT protocols, N-pathway<br />
|editor=Doerrier C<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
= References =<br />
<br />
{{#ask:[[Additional label::MitoFit 2022 NADH]] <br />
| mainlabel=Link<br />
|?Has title=Reference<br />
|?Was published in year=Year<br />
|?Has info=View<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Has title<br />
|order=ascending<br />
}}<br />
<br />
== Keywords ==<br />
{{Template:Keywords: NADH}}<br />
<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Mouse<br />
|tissues=Liver<br />
|preparations=Isolated mitochondria<br />
|topics=Redox state<br />
|couplingstates=LEAK, OXPHOS, ET<br />
|pathways=N<br />
|instruments=NextGen-O2k<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Theorell_1954_Acta_Chem_Scand&diff=226782Theorell 1954 Acta Chem Scand2022-05-03T08:02:10Z<p>Doerrier Carolina: </p>
<hr />
<div>{{Publication<br />
|title=Theorell H, Nygaard AP (1954) Kinetics and equilibria in flavoprotein systems. I. A fluorescence recorder and its application to a study of the dissociation of the old yellow enzyme and its resynthesis from riboflavin phosphate and protein. Acta Chem Scand 8:877–88.<br />
|info=[http://actachemscand.org/pdf/acta_vol_08_p0877-0888.pdf pdf Open Access]<br />
|authors=Theorell H, Nygaard AP<br />
|year=1954<br />
|journal=Acta Chem Scand<br />
|editor=Doerrier C<br />
}}<br />
{{Labeling<br />
|area=Instruments;methods<br />
|additional=<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Komlodi_2018b_EBEC2018&diff=226781Komlodi 2018b EBEC20182022-05-03T07:59:18Z<p>Doerrier Carolina: </p>
<hr />
<div>{{Abstract<br />
|title=Endogenous quinones sustain NADH oxidation by Complex I during anoxia, supporting substrate-level phosphorylation in mouse liver mitochondria.<br />
|info=[[EBEC2018]]<br />
|authors=Komlodi T, Ravasz D, Kitayev A, Hill C, Kiebish M, Doerrier C, Moore AL, Gnaiger E, Narain N, Seyfried TN, Adam-Vizi V, Chinopoulos C<br />
|year=2018<br />
|event=EBEC2018<br />
|abstract=Anoxia leads to over-reduction of mitochondrial coenzyme Q (Q, quinone) pools rendering Complex I unable to oxidize NADH, leading to a profound decrease in the matrix NAD<sup>+</sup>/NADH ratio. As a consequence, the function of matrix dehydrogenases is impaired. Yet, under certain anoxic conditions catabolism of metabolites converging through the α-ketoglutarate dehydrogenase Complex (KGDHC) is known to occur, yielding succinyl-CoA, in turn supporting substrate-level phosphorylation substantiated by succinate-CoA ligase [1].<br />
<br />
Mitochondrial respiration and NADH autofluorescence were measured simultaneously with the NextGen-O2k (Oroboros Instruments, Innsbruck, Austria), and the redox state of the Q-pool was detected with a three-electrode system implanted into the O2k (Oroboros Q2k).<br />
<br />
We show that in isolated mouse liver mitochondria Complex I utilizes endogenous quinones oxidizing NADH during anoxia. Untargeted metabolomic analysis of matrix metabolites of mitochondria subjected to respiratory arrest due to anoxia and in the presence of specific inhibitors of respiratory complexes infer showed that NAD<sup>+</sup> arising from Complex I is utilized by KGDHC yielding succinyl-CoA for succinate-CoA ligase, thus maintaining substrate-level phosphorylation during anoxia. Finally, by using custom-made 3D-printed plugs designed for standard fluorometric cuvettes, we show that under no conditions of respiratory arrest due to anoxia and/or pharmacological inhibition of the complexes did the mitochondria undergo swelling, which could potentially confound matrix metabolite estimations or bioenergetic parameters due to permeability transition.<br />
Our results highlight the importance of the availability of quinones in conjunction with the operation of Complex I in maintaining substrate-level phosphorylation during anoxia.<br />
|editor=[[Kandolf G]]<br />
|mipnetlab=AT Innsbruck Oroboros, UK Brighton Moore AL, HU Budapest Chinopoulos C<br />
}}<br />
== Affiliations ==<br />
:::: Komlódi T(4), Ravasz D(1), Kitayev A(2), Hill C, Kiebish M(2), Doerrier C(4), Moore AL(3), Gnaiger E(4,5), Narain N(2), Seyfried TN(6), Adam-Vizi V(1), Chinopoulos C(1)<br />
<br />
::::# Dept Medical Biochem, Semmelweis Univ, Budapest, Hungary<br />
::::# BERG LLC, Framingham, MA, USA<br />
::::# Dept Biochemistry Molecular Biology, School Life Sciences, Univ Sussex, Brighton, UK<br />
::::# Oroboros Instruments, Innsbruck, Austria<br />
::::# D. Swarovski Research Lab, Mitochondrial Physiology, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria<br />
::::# Biology Dept, Boston College, Chestnut Hill, Boston, MA, USA<br />
:::::: timea.komlodi@oroboros.at<br />
<br />
== References ==<br />
:::# Kiss G, Konrad C, Doczi J, Starkov AA, Kawamata H, Manfredi G, Zhang SF, Gibson GE, Beal MF, Adam-Vizi V, Chinopoulos C (2013) The negative impact of α-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation. FASEB J 27:2392-406.<br />
<br />
{{Labeling<br />
|area=Respiration<br />
|injuries=Permeability transition, Oxidative stress;RONS<br />
|organism=Mouse<br />
|tissues=Liver<br />
|preparations=Isolated mitochondria<br />
|topics=Q-junction effect<br />
|pathways=N<br />
|instruments=Oxygraph-2k, O2k-Fluorometer<br />
|additional=MitoFit 2022 NADH<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=MiPNet03.02_Chemicals-Media&diff=226780MiPNet03.02 Chemicals-Media2022-05-03T07:56:30Z<p>Doerrier Carolina: </p>
<hr />
<div>{{OROBOROS header page name}}<br />
{{Publication<br />
|title=Selected media and chemicals for respirometry with mitochondrial preparations.<br />
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/3/3c/MiPNet03.02_Chemicals-Media.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet03.02_Chemicals-Media.pdf Versions] [[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]]<br />
|authors=Oroboros<br />
|year=2016-08-30<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''Fontana-Ayoub M, Fasching M, Gnaiger E (2016) Selected media and chemicals for respirometry with mitochondrial preparations. Mitochondr Physiol Network 03.02(18):1-10.'''<br />
Different media for tissue preparation and respiration are used in investigations of mitochondrial function. Initial decisions on the composition of media and chemicals are decisive for long-term studies and crucial for comparability of results. As a guideline, we summarize an update of our experience with media and chemicals for high-resolution respirometry with isolated mitochondria, permeabilized cells, muscle fibres and tissue homogenates. Whereas optimization is necessary for specific experimental protocols, standardization will improve the comparability of results obtained in different laboratories. Efforts towards standardization are important for the advancement of mitochondrial physiology.<br />
:» Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]]<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
== Supplementary information ==<br />
::::» [[MiPNet09.12 O2k-Titrations]]; [[MitoPedia]]<br />
::::» [[Cytochrome c control factor]]<br />
::::» For calculations, see Excel file: [http://www.oroboros.at/?Protocols_titrations O2k-Titrations.xls]<br />
::::» For pH adjustment of BIOPS solution, note the temperature dependence:<br />
:::::::* at 0 °C pH = 7.1<br />
:::::::* at 23 °C pH = 6.75<br />
<br />
== Cited by ==<br />
{{Template:Cited by Cardoso 2021 MitoFit MgG}}<br />
{{Template:Cited by Komlodi 2021 MitoFit Tissue normoxia}}<br />
<br />
{{Labeling<br />
|area=Instruments;methods<br />
|instruments=O2k-Protocol<br />
|additional=O2k-chemicals and media, Uncoupling, ADP, ATP, MitoFit 2021 MgG, MitoFit 2021 Tissue normoxia, MitoFit 2022 NADH<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=De_Jager_2017_Adv_Exp_Med_Biol&diff=226779De Jager 2017 Adv Exp Med Biol2022-05-03T07:53:56Z<p>Doerrier Carolina: </p>
<hr />
<div>{{Publication<br />
|title=de Jager TL, Cockrell AE, Du Plessis SS (2017) Ultraviolet Light Induced Generation of Reactive Oxygen Species. Adv Exp Med Biol 996:15-23.<br />
|info=[https://pubmed.ncbi.nlm.nih.gov/29124687/ PMID: 29124687]<br />
|authors=de Jager TL, Cockrell AE, Du Plessis SS<br />
|year=2017<br />
|journal=Adv Exp Med Biol<br />
|abstract=As ultraviolet (UV) radiation is naturally and ubiquitously emitted by the sun, almost everyone is exposed to it on a daily basis, and it is necessary for normal physiological function. Human exposure to solar UV radiation thus has important health implications. The generation of reactive oxygen species (ROS) by UV radiation is one of the mechanisms through which UV light can manifest its possible detrimental effects on health. When an imbalance develops due to ROS generation exceeding the body's antioxidant defence mechanisms, oxidative stress can develop. Oxidative stress can lead to cellular damage (e.g. lipid peroxidation and DNA fragmentation), apoptosis and cell death. Broadly UV can induce ROS by affecting the cellular components directly or by means of photosensitization mechanisms. More specifically UV light can induce ROS by affecting the enzyme catalase and up-regulating nitric oxide synthase (NOS) synthesis. It may also cause a decrease in protein kinase C (PKC) expression leading to increased ROS production. UVR is capable of modifying DNA and other chromophores resulting in elevated ROS levels. The effects of raised ROS levels can vary based on the intracellular oxidant status of the cell. It is therefore important to protect yourself against the potentially harmful effects of UV light as it can lead to pathological UV-induced ROS production.<br />
|keywords=antioxidants, catalase, nitric oxide, oxidant, protein kinase C, reactive oxygen species<br />
|editor=Doerrier C<br />
}}<br />
{{Labeling<br />
|additional=MitoFit 2022 NADH<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=De_Jager_2017_Adv_Exp_Med_Biol&diff=226778De Jager 2017 Adv Exp Med Biol2022-05-03T07:53:24Z<p>Doerrier Carolina: Created page with "{{Publication |title=de Jager TL, Cockrell AE, Du Plessis SS (2017) Ultraviolet Light Induced Generation of Reactive Oxygen Species. Adv Exp Med Biol 996:15-23. |info=[https:/..."</p>
<hr />
<div>{{Publication<br />
|title=de Jager TL, Cockrell AE, Du Plessis SS (2017) Ultraviolet Light Induced Generation of Reactive Oxygen Species. Adv Exp Med Biol 996:15-23.<br />
|info=[https://pubmed.ncbi.nlm.nih.gov/29124687/ PMID: 29124687]<br />
|authors=de Jager TL, Cockrell AE, Du Plessis SS<br />
|year=2017<br />
|journal=Adv Exp Med Biol<br />
|abstract=As ultraviolet (UV) radiation is naturally and ubiquitously emitted by the sun, almost everyone is exposed to it on a daily basis, and it is necessary for normal physiological function. Human exposure to solar UV radiation thus has important health implications. The generation of reactive oxygen species (ROS) by UV radiation is one of the mechanisms through which UV light can manifest its possible detrimental effects on health. When an imbalance develops due to ROS generation exceeding the body's antioxidant defence mechanisms, oxidative stress can develop. Oxidative stress can lead to cellular damage (e.g. lipid peroxidation and DNA fragmentation), apoptosis and cell death. Broadly UV can induce ROS by affecting the cellular components directly or by means of photosensitization mechanisms. More specifically UV light can induce ROS by affecting the enzyme catalase and up-regulating nitric oxide synthase (NOS) synthesis. It may also cause a decrease in protein kinase C (PKC) expression leading to increased ROS production. UVR is capable of modifying DNA and other chromophores resulting in elevated ROS levels. The effects of raised ROS levels can vary based on the intracellular oxidant status of the cell. It is therefore important to protect yourself against the potentially harmful effects of UV light as it can lead to pathological UV-induced ROS production.<br />
|keywords=antioxidants, catalase, nitric oxide, oxidant, protein kinase C, reactive oxygen species<br />
|editor=Doerrier C<br />
}}<br />
{{Labeling}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Chance_1955_J_Biol_Chem&diff=226776Chance 1955 J Biol Chem2022-05-03T07:46:37Z<p>Doerrier Carolina: </p>
<hr />
<div>{{Publication<br />
|title=Chance B, Williams GR, Holmes WF, Higgins J (1955) Respiratory enzymes in oxidative phosphorylation. V. Mechanism for oxidative phosphorylation. J Biol Chem 217:439-51.<br />
|info=[http://www.ncbi.nlm.nih.gov/pubmed/13271406 PMID: 13271406 Open Access]<br />
|authors=Chance B, Williams GR, Holmes WF, Higgins J<br />
|year=1955<br />
|journal=J Biol Chem<br />
|abstract=In a preceding paper (1) we have used, as an explanation of spectroscopic changes observed in oxidative phosphorylation, an analogy between antimycin A inhibition of the respiratory chain and the inhibition of mitochondrial respiration which is brought about by the exhaustion of added ADP. The latter process causes a 10-fold decrease of respiration rate and, in addition, changes in the steady state oxidation-reduction levels of spectroscopically detectable members of the respiratory chain involved in oxidative phosphorylation. In this paper we describe three types of inhibiting reactions that can affect steady state levels in the respiratory chain and analyze one of these mechanisms in detail by analytical and analogue computer methods. It has been possible to formulate a few general relationships between changes in steady state oxidation-reduction levels caused by the State 3 to 4 transition and the sites involved in oxidative phosphorylation.<br />
|editor=Doerrier C<br />
}}<br />
{{Labeling<br />
|area=Respiration<br />
|additional=<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Chance_1962_Science&diff=226775Chance 1962 Science2022-05-03T07:46:28Z<p>Doerrier Carolina: </p>
<hr />
<div>{{Publication<br />
|title=Chance B, Cohen P, Jobsis F, Schoener B (1962) Intracellular oxidation-reduction states in vivo. Science 137:499-508.<br />
|info=[https://pubmed.ncbi.nlm.nih.gov/13878016/ PMID: 13878016]<br />
|authors=Chance B, Cohen P, Jobsis F, Schoener B<br />
|year=1962<br />
|journal=Science<br />
|abstract=It now appears to be possible to continuously record changes in intracellular oxidation-reduction levels in terms of the fluorescence of reduced pyridine nucleotide in mitochondria of various tissues and organs in situ. Studies of kidney and brain cortex in the rat show that changes in fluorescence are not measurably affected by the presence of oxyhemoglobin. Nitrogen, sulfide, cyanide, and carbon monoxide cause increases in fluorescence to very nearly the same levels, and the increases are attributed to larger reduction of mitochondrial diphosphopyridine nucleotide. Amytal at a low blood concentration causes increased reduction in the kidney cortex, and at a high blood concentration, in the brain cortex. The qualitative response of the pyridine nucleotide to low oxygen concentrations shows the brain to be more sensitive than the kidney. The first measurable increase in pyridine nucleotide reduction observed on the brain occurs at a concentration of inspired oxygen of 8 percent. Breathing stops when the percentage increase of pyridine nucleotide reduction on the brain reaches about 90; at this point the percentage increase for the kidney is only about 30. This difference corresponds roughly to a tenfold difference in oxygen tension. Half-maximal increase in pyridine nucleotide reduction on the brain occurs at a concentration of inspired oxygen of about 4 percent and corresponds to an intracellular oxygen tension of about 0.2 mm.<br />
|editor=Doerrier C<br />
}}<br />
{{Labeling<br />
|area=Instruments;methods<br />
|additional=<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Cardoso_2021_BEC_MgG&diff=226774Cardoso 2021 BEC MgG2022-05-03T07:45:34Z<p>Doerrier Carolina: </p>
<hr />
<div>{{BEC}}<br />
[[File:BEC-exlibris.png|right|290px|Bioenergetics Communications|link=https://www.bioenergetics-communications.org/index.php/bec/index]]<br />
{{Publication<br />
|title=Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001<br />
|info=[[File:OpenAccess-downloadPDF.png|240px||link=https://www.bioenergetics-communications.org/index.php/bec/article/view/cardoso_2021_mgg/20 |BEC pdf]] '''BEC2021.1.''' ''Published online'' 2021-06-30<br /><br /><br />
|authors=Cardoso Luiza HD, Doerrier Carolina, Gnaiger Erich<br />
|year=2021-06-30<br />
|journal=Bioenerg Commun<br />
|abstract=[[File:BEC.png|25px|link=https://www.bioenergetics-communications.org/index.php/bec/article/view/cardoso_2021_mgg/20]] [https://www.bioenergetics-communications.org/index.php/bec/article/view/cardoso_2021_mgg/20 doi:10.26124/bec:2021-0001]<br />
[[File:MgG BEC 2021.1.jpg|550px|right|link=https://doi.org/10.26124/bec:2021-0001]]<br />
<br />
For the advanced study of mitochondrial function, high-resolution respirometry is extended by fluorometric measurement of ATP production using the fluorophore Magnesium Green™ (MgG). A common problem with several fluorescent dyes is the inhibition of mitochondrial respiration. In the present study, a coupling control protocol was applied in combination with MgG to measure ATP production simultaneously with respiration for calculation of P»/O<sub>2</sub> ratios. MgG at 1.1 µM did not affect respiration through the NADH-linked and succinate-linked pathways. Respiration was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG.<br />
<br><br><br />
<br />
|keywords=ATP, ATP production, high-resolution respirometry, Magnesium Green, mitochondria, oxidative phosphorylation, fluorometry, FluoRespirometry<br />
|editor=Gnaiger E<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-6392-9229]] Cardoso Luiza HD, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich<br />
<br /><br /><br />
<br />
__TOC__<br />
== Data availability ==<br />
:::: Original files are available Open Access at Zenodo repository: [https://doi.org/10.5281/zenodo.4916141 10.5281/zenodo.4916141] <br />
<br />
== Support ==<br />
[[File:Template NextGen-O2k.jpg|right|400px|link=NextGen-O2k]]<br />
:::: Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770. An initiative of the MitoEAGLE Task Group of the Mitochondrial Physiology Society.<br />
<br />
== Keywords ==<br />
{{Template:Keywords: ATP production}}<br />
<br />
== References (Open Access versus Paywall) ==<br />
:: <big>'''References - Open Access'''</big><br />
{{#ask:[[Additional label::MitoFit 2021 MgG]]<br />
| mainlabel=Link<br />
|?Has title=Reference<br />
|?Was published in year=Year<br />
|?Has info=View<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Has title<br />
|order=ascending<br />
}}<br />
<br />
:: <big>'''References - Paywall'''</big><br />
{{#ask:[[Additional label::MitoFit 2021 MgG-PW]]<br />
| mainlabel=Link<br />
|?Has title=Reference<br />
|?Was published in year=Year<br />
|?Has info=View<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Has title<br />
|order=ascending<br />
}}<br />
<br />
== Cited by ==<br />
{{Template:Cited by Gnaiger 2021 Bioenerg Commun}}<br />
<br />
== Preprint ==<br />
:::: [[Cardoso 2021 MitoFit MgG]]<br />
<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Mouse<br />
|tissues=Heart<br />
|preparations=Isolated mitochondria<br />
|topics=ATP production, Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, OXPHOS, ET<br />
|pathways=N, S, ROX<br />
|instruments=Oxygraph-2k, O2k-Fluorometer, NextGen-O2k<br />
|additional=SUIT-006, MgG, MitoEAGLEPublication, BEC2021.5, MitoFit 2022 NADH<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Doerrier_2022_MitoFit_NADH&diff=226773Doerrier 2022 MitoFit NADH2022-05-03T07:36:32Z<p>Doerrier Carolina: </p>
<hr />
<div>{{Publication<br />
|title=Doerrier C, Cardoso LHD, Gnaiger E (2022) Simultaneous measurement of oxygen consumption and NAD(P)H/NAD(P)<sup>+</sup>-redox state in isolated mitochondria. (in prep)<br />
|info=<br />
|authors=<br />
|year=<br />
|journal=<br />
|abstract=<br />
|keywords=NADH fluorescence, NAD-pool, N-junction, NAD-redox state, high-resolution respirometry HRR, isolated mitochondria, mouse liver mitochondria, SUIT protocols, N-pathway<br />
|editor=Doerrier C<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
= References =<br />
<br />
{{#ask:[[Additional label::MitoFit 2022 NADH]] <br />
| mainlabel=Link<br />
|?Has title=Reference<br />
|?Was published in year=Year<br />
|?Has info=View<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Has title<br />
|order=ascending<br />
}}<br />
<br />
== Keywords ==<br />
{{Template:Keywords: NADH}}<br />
<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|instruments=NextGen-O2k<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Bombaca_2022_MitoFit&diff=226695Bombaca 2022 MitoFit2022-04-28T12:45:22Z<p>Doerrier Carolina: </p>
<hr />
<div> In prep<br />
{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Bombaça ACS, Menna-Barreto RFS (2022) Mitochondrial plasticity in trypanosomatids as a stress adaptation mechanism. MitoFit Preprints 2022.16. https://doi.org/10.26124/mitofit:2022-0016<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/c/c0/Bombaca_2022_MitoFit.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/c/c0/Bombaca_2022_MitoFit.pdf Mitochondrial plasticity in trypanosomatids as a stress adaptation mechanism]<br/><br />
|authors=Bombaca Ana Cristina S, Menna Barreto Rubem<br />
|year=2022-04-28<br />
<br />
|journal=MitoFit Prep<br />
|abstract=[[File:Bombaca 2022 MitoFit graphical abstract.png|right|300px|Graphical abstract]] Neglected tropical diseases impact more than a billion people globally, with millions of them at risk of infection by parasites of the Trypanosomatidae family. The need to colonize different environments in their hosts means that trypanosomatids are constantly subjected to stress situations, among which the presence of reactive oxygen (ROS) and nitrogen (RNS) species, requiring intense metabolic remodeling to ensure the parasites survival in hostile environments. Additionaly to the classical role in bioenergetics, mitochondrion has a decisive contribution to the oxidative stress, due to the electron leakage from the electron transfer system (ETS). The presence of several functional peculiarities made the mitochondrion of trypanosomatids an unique organelle, considered an excellent target for drug intervention. Some trypanosomatids such as ''Leishmania'' spp. can avoid the microbicidal mechanisms of the host cells, exhibiting a profile of natural resistance to oxidative and nitrosative stresses. Here, we discussed data about mitochondrial susceptibility and adaptative processes obtained by our group in the last 17 years. Mechanistic proposals of preclinical drugs was reviewed, as well as different pathways associated with metabolic and mitochondrial remodeling during the life cycle of trypanosomatids, including the possible biological role of ROS and RNS resistance and its impact on the interaction with vertebrate and invertebrate hosts. <br><br><br />
|keywords=trypanosomatids, mitochondrion, bioenergetics, oxidative stress, chemotherapy<br />
|editor=Tindle-Solomon L<br />
<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-8573-6882]] Bombaça Ana Cristina S, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-1352-0641]] Menna-Barreto Rubem FS <br />
<br />
<br />
{{Labeling<br />
|area=Comparative MiP;environmental MiP<br />
|diseases=Other<br />
|organism=Protists<br />
|additional=Bioblast 2022, Trypanosoma<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Bombaca_2022_MitoFit&diff=226694Bombaca 2022 MitoFit2022-04-28T12:44:34Z<p>Doerrier Carolina: </p>
<hr />
<div> In prep<br />
{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Bombaça ACS, Menna-Barreto RFS (2022) Mitochondrial plasticity in trypanosomatids as a stress adaptation mechanism. MitoFit Preprints 2022.16. https://doi.org/10.26124/mitofit:2022-0016<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/c/c0/Bombaca_2022_MitoFit.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/c/c0/Bombaca_2022_MitoFit.pdf Mitochondrial plasticity in trypanosomatids as a stress adaptation mechanism]<br/><br />
|authors=Bombaca Ana Cristina S, Menna Barreto Rubem<br />
|year=2022-04-28<br />
<br />
|journal=MitoFit Prep<br />
|abstract=[[File:Bombaca 2022 MitoFit graphical abstract.png|right|300px|Graphical abstract]] Neglected tropical diseases impact more than a billion people globally, with millions of them at risk of infection by parasites of the Trypanosomatidae family. The need to colonize different environments in their hosts means that trypanosomatids are constantly subjected to stress situations, among which the presence of reactive oxygen (ROS) and nitrogen (RNS) species, requiring intense metabolic remodeling to ensure the parasites survival in hostile environments. Additionaly to the classical role in bioenergetics, mitochondrion has a decisive contribution to the oxidative stress, due to the electron leakage from the electron transfer system (ETS). The presence of several functional peculiarities made the mitochondrion of trypanosomatids an unique organelle, considered an excellent target for drug intervention. Some trypanosomatids such as ''Leishmania'' spp. can avoid the microbicidal mechanisms of the host cells, exhibiting a profile of natural resistance to oxidative and nitrosative stresses. Here, we discussed data about mitochondrial susceptibility and adaptative processes obtained by our group in the last 17 years. Mechanistic proposals of preclinical drugs was reviewed, as well as different pathways associated with metabolic and mitochondrial remodeling during the life cycle of trypanosomatids, including the possible biological role of ROS and RNS resistance and its impact on the interaction with vertebrate and invertebrate hosts. <br><br><br />
|keywords=trypanosomatids, mitochondrion, bioenergetics, oxidative stress, chemotherapy<br />
|editor=Tindle-Solomon L<br />
<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-8573-6882]] Bombaça Ana Cristina S, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-1352-0641]] Menna-Barreto Rubem FS <br />
<br />
<br />
{{Labeling<br />
|area=Comparative MiP;environmental MiP<br />
|diseases=other<br />
|organism=Protists<br />
|additional=Bioblast 2022, Trypanosoma<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=MitoKIN&diff=226249MitoKIN2022-04-11T12:59:47Z<p>Doerrier Carolina: </p>
<hr />
<div>{{Template:OROBOROS header page name}}<br />
<br />
<big><big>'''MitoKIN - Systematic barcoding of kinase-controlled mitochondrial function'''</big></big><br />
<br />
[[File:FFG-logo.png|right|200px|FFG]]<br />
<br />
__TOC__<br />
<br />
<br />
== MitoKIN: FFG BRIDGE 1 ==<br />
::::* FFG BRIDGE 1 30. Call - Project No. 877163, University of Innsbruck, Institute of Biochemistry<br />
::::* '''Duration''': 36 months<br />
::::* '''Start''': 2020-02-03<br />
<br />
<br />
<br />
[[File:MitoKIN O2ks.jpg|right|400px|thumb|Dr. Eduard Stefan and Dr. Omar Torres-Quesada receiving the O2k instruments for the MitoKIN project.]]<br />
== About MitoKIN ==<br />
<br />
<br />
:::: Led by the Institute of Biochemistry at the University of Innsbruck, the MitoKIN project aims to connect specific/broad kinase activities to precise metabolic functions (mitochondria) relevant for cell proliferation or survival. Applied inhibitors could then be used to barcode involved kinase pathways that change the metabolome by boosting or preventing mitochondrial functions. <br />
<br />
=== Aims ===<br />
:::: The MitoKIN project has three major aims:<br />
<br />
:::::: 1. Impact of specific/general kinase inhibition on metabolism & mitochondrial function.<br />
:::::: 2. Perturbation of PKA signaling in cancer cells (high cAMP levels). <br />
:::::: 3. Perturbation of PKA signaling in neurodegenerative disease models (low cAMP levels).<br />
<br />
<br />
== Coordinator ==<br />
::::* [[Stefan E| Eduard Stefan, Ph.D.]] - Institute of Biochemistry, University of Innsbruck - https://www.uibk.ac.at/biochemistry/people/stefan/<br />
<br />
<br />
== Network ==<br />
[[File:MitoKIN group photo.jpg|right|400px|thumb|Back row, left to right: Eduard, Verana, Carolina, Erich; front row left to right: Lisa, Omar]]<br />
=== Participants === <br />
::::'''Institute of Biochemistry, University of Innsbruck''' <br />
::::* [[Stefan E| '''Eduard Stefan, Ph.D.''']] - Project Coordinator, WP 1 & 4 Leader<br />
::::* [[Torres-Quesada O| '''Omar Torres-Quesada, Ph.D.''']] - Project Coordinator, WP 2-4 Leader<br />
<br />
:::: '''Oroboros Instruments GmbH''' - [https://www.oroboros.at/ www.oroboros.at]<br />
::::* [[Gnaiger E| '''Erich Gnaiger, Ph.D.''']] - PI<br />
::::* [[Doerrier C| '''Carolina Doerrier, Ph.D.''']] - WP 2 Leader<br />
::::* [[Laner V| '''Verena Laner''']] - Oroboros project management<br />
::::* [[Tindle-Solomon L| '''Lisa Tindle-Solomon''']] - Oroboros project management<br />
<br />
<br />
<br />
<br />
<br />
== Oroboros project involvement ==<br />
:::: Oroboros Instruments will provide both infrastructure and the expertise required to conduct the respirometric measurements of the project.<br />
<br />
:::: The Institute of Biochemistry has been equipped with 3 O2ks for the duration of the project.<br />
<br />
== MitoKIN publications, preprints, abstracts and events ==<br />
{{#ask:[[Category:Publications]] [[Additional label::MitoKIN]]<br />
|?Was published in year=When<br />
|?Has title=Where<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=ascending<br />
}}<br />
<br />
<br />
== Links ==<br />
<br />
'''News and articles'''<br />
<br />
:::: [https://www.uibk.ac.at/newsroom/krebs-kinasen-und-zellkraftwerke.html.de Krebs, Kinasen und Zellkraftwerke] www.uibk.ac.at, 2020-02-04<br />
<br />
<br />
<br />
== Support ==<br />
This project has received funding from the Austrian Reserach Promotion Funding Agency (FFG). [[File:FFG-logo.png|left|100px]]</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Doerrier_Carolina&diff=226248Doerrier Carolina2022-04-11T12:56:40Z<p>Doerrier Carolina: </p>
<hr />
<div>{{EAGLE<br />
|COST= Member<br />
|COST WG1 = WG1<br />
|COST WG2 = WG2<br />
|COST WG3 = WG3<br />
|COST WG4 = WG4<br />
|COST ECI= ECI<br />
}}<br />
: [[Management_Committee_MitoEAGLE#MC_Substitutes|MC Substitute]] - [[Management Committee MitoEAGLE]]<br />
{{NextGen-O2k H2020-support}}<br />
{{Person<br />
|lastname=Doerrier Velasco<br />
|firstname=Carolina<br />
|title=PhD.<br />
|institution=::::::::::::::::[[File:DoerrierC.JPG|right|150px|Carolina Doerrier]]<br />
Scientific Motive Force<br />
<br />
Oroboros Instruments<br />
* Mitochondrial Physiology Specialist ([[O2k-Innovation description|O2k-Innovation]])<br />
* Mitochondrial Application Specialist<br />
* [[NextGen-O2k]]<br />
* [[K-Regio_MitoFit|K-Regio MitoFit]]<br />
<br />
<br />
Background: [[ES Granada Acuna-Castroviejo D]]<br />
<br />
Carolina joined [[Oroboros_Contact |Oroboros Instruments]] in October 2014.<br />
|address=Schoepfstrasse 18<br />
|area code=6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress=carolina.doerrier@oroboros.at<br />
|weblink=[http://www.oroboros.at www.oroboros.at], [[Bioblast|www.bioblast.at]], [http://www.mitophysiology.org www.mitophysiology.org], [https://www.researchgate.net/profile/Carolina_Doerrier_Velasco ResearchGate profile], ORCID:[[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]]<br />
}}<br />
<br />
__TOC__<br />
== Bioenergetics Communications ==<br />
::::* Carolina Doerrier is Section Editor of the journal '''[[Bioenergetics Communications]]'''<br />
:::::'''Keywords:''' O2k-FluoRespirometry, oxidative stress, permeabilized muscle fibers, NAD(P)H, sepsis, aging, melatonin<br />
==[[Oroboros Contact| Oroboros-team]]==<br />
:* Previous laboratory: [[ES Granada Acuna-Castroviejo D]]<br />
:* Collaboration with the [[AT_Innsbruck_Gnaiger E|Gnaiger Lab]] in Innsbruck during a 3-months scholarship (Sep - Dec 2011), in the frame of the K-Regio project ''[[MitoCom_O2k-Fluorometer| MitoCom Tyrol]]''.<br />
:* Collaboration with the [[US FL Orlando Goodpaster BH|Goodpaster Lab]] in Orlando (2017).<br />
:* MitoEAGLE Short-Term Scientific Mission at [[ES Barcelona Garcia-Roves PM]] in Barcelona (2018).<br />
:* Guest tutor at [[IOC65]] and [[IOC68]]. <br />
:* Tutor at [[IOC100]], [[IOC104]], [[IOC106]], [[IOC110]], [[IOC112]], [[IOC114]], [[IOC115]], [[IOC116]], [[IOC120]], [[IOC122]], [[IOC130]], [[IOC132]], [[IOC133]], [[IOC137]], [[IOC139]], [[IOC144]] and [[IOC145]].<br />
:* Current laboratory: [[AT Innsbruck Oroboros]]<br />
== Bioblast Editorial Board ==<br />
<br />
:* Bioblast national editor [[MiPNet Laboratories Spain]]<br />
:* [[Library of protocols]]<br />
<br />
== MitoEAGLE Short-Term Scientific Mission ==<br />
****: [[Short-Term Scientific Missions MitoEAGLE#STSM_Grant_Period_2 |STSM Grant Period 2]]<br />
::: '''Work Plan summary'''<br />
:::: WORKPLAN SUMMARY Different experimental aspects (e.g. purity of the preparations, environmental humidity which could affect the wet weight of the muscle, chemicals) will be evaluated during the STSM in order to clarify the sources of variability found in the MitoEAGLE WG2 pilot study. The preparation of the samples will be performed according the SOP established for the MitoEAGLE WG2 pilot study. Permeabilized fibers from soleus of C57BL6/J mice will be used. Mitochondrial respiration will be assessed by O2k high-resolution respirometry (HRR). The Substrate- Uncoupler-Inhibitor Titration (SUIT) protocol used in the MitoEAGLE WG2 pilot study for obtaining mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers, will be used to perform the experiments during the STSM at the Department of Physiological Sciences II in the Faculty of Medicine (Barcelona University, Barcelona, Spain).<br />
<br />
<br />
<br />
== Participated at ==<br />
::::* [[MiPNet 26.16 NextGen-O2k Summit 2021 Virtual]]<br />
::::* [[2020 PaduaMuscleDays Padua IT]]<br />
::::* [[FAT4BRAIN School IOC147 Virtual Event]]<br />
::::* [[MiPNet25.06 IOC145 Innsbruck AT|IOC145 Innsbruck AT]]<br />
::::* [[MiPNet25.03 IOC144 Innsbruck AT|IOC144 Innsbruck AT]]<br />
::::* [[MiP2019/MitoEAGLE Belgrade RS|MitoEAGLE 2019 Belgrade RS]]<br />
::::* [[MiP2019/MitoEAGLE Belgrade RS]]<br />
::::* [[FEBS Workshop Ageing 2019 Innsbruck AT]]<br />
::::* [[Mitochondrial Physiology ‐ from Organelle to Organism 2019 Copenhagen DK]]<br />
::::* [[MiPschool Coimbra 2019|MiP/MitoEAGLE Training School 2019 Coimbra PT]]<br />
::::* [[IOC139| IOC139 Schroecken AT]]<br />
::::* [[Life Science PhD Meeting 2019 Innsbruck AT]]<br />
::::* [[MitoEAGLE Obergurgl 2019-01-31| MitoEAGLE 2019 Obergurgl AT]]<br />
::::* [[IOC137|IOC137 Innsbruck AT]]<br />
::::* [[MitoEAGLE_Innsbruck_2018-11-19| MitoEAGLE 2018 Innsbruck AT]]<br />
::::* [[IOC133|IOC133 Innsbruck AT]]<br />
::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]<br />
::::* [[EBEC2018 Budapest HU]]<br />
::::* [[MitoEAGLE Copenhagen 2018| MitoEAGLE 2018 Copenhagen DK]]<br />
::::* [[MiPNet23.06 IOC130 Schroecken AT| IOC130 Schroecken AT]]<br />
::::* [[ESCI 2018 Barcelona ES]]<br />
::::* [[MitoFit Workshop ATP 2017 Innsbruck AT]]<br />
::::* [[MiP2017/MitoEAGLE Hradec Kralove CZ|MitoEAGLE 2017 Hradec Kralove CZ]]<br />
::::* [[MiPNet22.01 IOC122 Schroecken AT]]<br />
::::* [[MitoEAGLE Obergurgl 2017| MitoEAGLE 2017 Obergurgl AT]]<br />
::::* [[MiPschool Obergurgl 2017| MiPschool 2017 Obergurgl AT]]<br />
::::* [[MitoEAGLE Barcelona 2017| MitoEAGLE 2017 Barcelona ES]]<br />
::::* [[MiPNet22.04 IOC120 Barcelona ES | IOC120 Barcelona ES]]<br />
::::* [[Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE| Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE]]<br />
::::* [[MiPNet21.11 IOC116 Innsbruck AT|IOC116 Innsbruck AT]]<br />
::::* [[IOC115 | IOC115 Schroecken AT]]<br />
::::* [[MiPNet21.19 IOC114 Innsbruck AT | IOC114 Innsbruck AT]]<br />
::::* [[MitoEAGLE Verona 2016| MitoEAGLE 2016 Verona IT]]<br />
::::* [[MitoFit Science Camp 2016 Kuehtai AT]]<br />
::::* [[MiPNet21.15 IOC112 Kuehtai AT|IOC112 Kuehtai AT]]<br />
::::* [[MiPNet21.04 IOC110 Melbourne AU|IOC110 Melbourne AU]]<br />
::::* [[Research to Practice 2016 Melbourne AU]]<br />
::::* [[Bioblast 2012 | Bioblast 2012 Innsbruck AT]]<br />
::::* [[MiPNet20.10 IOC106 Schroecken|IOC106 Schroecken AT]]<br />
::::* [[MiPNet20.05 IOC104 Greenville |IOC104 Greenville NC US]]<br />
::::* [[IOC100 | IOC100 Schroecken AT]]<br />
::::* [[IOC68 | IOC68 Schroecken AT]]<br />
::::* [[IOC65 | IOC65 Schroecken AT]]<br />
::::* [[MiPNet14.04 IOC51 | IOC51 Schroecken AT]]<br />
::::* [[MiP2015 | MiP2015 Pec pod Snezkou CZ]]<br />
::::* [[MiPschool Greenville 2015 | MiPschool 2015 Greenville NC US]]<br />
<br />
{{Labelingperson<br />
|field of research=Basic<br />
|topics=[[High-resolution respirometry]], [[Mitochondria]], [[Melatonin]], [[Reactive oxygen species]], [[Reactive nitrogen species]], [[Sepsis]]<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit_Kinase&diff=226247Torres-Quesada 2022 MitoFit Kinase2022-04-11T12:54:31Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada Omar, Strich Sophie, Stefan Eduard (2022) Kinase perturbations redirect mitochondrial function. MitoFit Prep 2022.11. https://doi.org/10.26124/mitofit:2022-0011<br />
|info= [[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/9/91/Torres-Quesada_2022_MitoFit_Kinase.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/9/91/Torres-Quesada_2022_MitoFit_Kinase.pdf Kinase perturbations redirect mitochondrial function]<br/> <br />
|authors=Torres-Quesada Omar, Strich Sophie, Stefan Eduard<br />
|year=2022-04-11<br />
|journal=MitoFit Prep<br />
|abstract=Protein kinases take the center stage in numerous signaling pathways by phosphorylating compartmentalized protein substrates for controlling cell proliferation, cell cycle and metabolism. Kinase dysfunctions have been linked to numerous human diseases such as cancer. This has led to the development of kinase inhibitors which aim to target oncogenic kinase activities. The specificity of the cancer blockers depends on the range of targeted kinases. Therefore, the question arises of how cell-type-specific off-target effects impair the specificities of cancer drugs. Blockade of kinase activities has been shown to converge on the energetic organelle, the mitochondria. In this review, we highlight examples of selected major kinases which impact mitochondrial signaling. Further, we discuss pharmacological strategies to target kinase activities which are linked to cancer progression and redirecting mitochondrial function. Finally, we propose that cell-based recordings of mitochondrial bioenergetic states might predict off-target or identify specific on-target effects of kinase inhibitors.<br><br><br />
|keywords=Kinases, signaling, mitochondria, kinase inhibitors, cancer, drug off-target effects <br />
|editor=Doerrier C<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, <br />
[[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
<br />
{{Labeling<br />
|area=Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|additional=MitoKIN<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit&diff=226246Torres-Quesada 2022 MitoFit2022-04-11T12:52:59Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines. MitoFit Preprints 2022.8. https://doi.org/10.26124/mitofit:2022-0008<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines]<br/><br />
|authors=Torres-Quesada Omar, Doerrier Carolina, Strich Sophie, Gnaiger Erich, Stefan Eduard<br />
|year=2022-04-01<br />
|journal=MitoFit Prep<br />
|abstract=Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. The simplicity of these models facilitates the reproducible manipulation of cultured cells. Limitations, however, relate to cell growth in a monolayer using standard cell culture media. Although such media are in use for decades, their formulations do not mimic the composition of the originating human cell environment in physiological or pathological conditions. In this study, we selected three frequently-used cancer cell lines SW620 (colon cancer), MCF7 (breast cancer), and A375 (melanoma) to determine the impact of a newly formulated human plasma-like medium (HPLM) on cell proliferation and mitochondrial function. Mitochondrial bioenergetic profiles were analyzed by high-resolution respirometry, revealing metabolic reprogramming in cells cultured in standard and HPLM media. Notably, the culturing of differentially proliferating cells in HPLM affected key mechanism of cellular respiration. All three cancer cell lines displayed an amending bioenergetic profile, particularly related to mitochondrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinically prescribed cancer drug and kinase inhibitor sunitinib. These findings highlight that cell culture media redirect mitochondrial function and affect cancer drug sensitivities. To advance the translational potential of cell culture models we propose to prioritize media with a human plasma-like composition for analyzing bioenergetic profiles and for determining target-oriented drug efficacies. <br><br><br />
|keywords=Cancer cells, cell respiration, mitochondrial function, HPLM, classical media, metabolism, cell culture media, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residual O2 consumption, bioenergetic cluster analysis BCA<br />
|editor=Tindle-Solomon L<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
__TOC__<br />
== Data availability ==<br />
:::: Original files are available Open Access at Zenodo repository: https://doi.org/10.5281/zenodo.6402435<br />
<br />
== Support ==<br />
:::: This work was founded by the projects 877163 for the Austrian FFG-Bridge program [[MitoKIN]], the Austrian Science Fund (FWF; P27606, P30441, P32960, P35159) and the Tyrolean Cancer Society.<br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, ROUTINE, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA, MitoKIN<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit&diff=226245Torres-Quesada 2022 MitoFit2022-04-11T12:52:16Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines. MitoFit Preprints 2022.8. https://doi.org/10.26124/mitofit:2022-0008<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines]<br/><br />
|authors=Torres-Quesada Omar, Doerrier Carolina, Strich Sophie, Gnaiger Erich, Stefan Eduard<br />
|year=2022-04-01<br />
|journal=MitoFit Prep<br />
|abstract=Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. The simplicity of these models facilitates the reproducible manipulation of cultured cells. Limitations, however, relate to cell growth in a monolayer using standard cell culture media. Although such media are in use for decades, their formulations do not mimic the composition of the originating human cell environment in physiological or pathological conditions. In this study, we selected three frequently-used cancer cell lines SW620 (colon cancer), MCF7 (breast cancer), and A375 (melanoma) to determine the impact of a newly formulated human plasma-like medium (HPLM) on cell proliferation and mitochondrial function. Mitochondrial bioenergetic profiles were analyzed by high-resolution respirometry, revealing metabolic reprogramming in cells cultured in standard and HPLM media. Notably, the culturing of differentially proliferating cells in HPLM affected key mechanism of cellular respiration. All three cancer cell lines displayed an amending bioenergetic profile, particularly related to mitochondrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinically prescribed cancer drug and kinase inhibitor sunitinib. These findings highlight that cell culture media redirect mitochondrial function and affect cancer drug sensitivities. To advance the translational potential of cell culture models we propose to prioritize media with a human plasma-like composition for analyzing bioenergetic profiles and for determining target-oriented drug efficacies. <br><br><br />
|keywords=Cancer cells, cell respiration, mitochondrial function, HPLM, classical media, metabolism, cell culture media, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residual O2 consumption, bioenergetic cluster analysis BCA<br />
|editor=Tindle-Solomon L<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
__TOC__<br />
== Data availability ==<br />
:::: Original files are available Open Access at Zenodo repository: https://doi.org/10.5281/zenodo.6402435<br />
<br />
== Support ==<br />
:::: This work was founded by the projects 877163 for the Austrian FFG-Bridge program [[MitoKIN]], the Austrian Science Fund (FWF; P27606, P30441, P32960, P35159) and the Tyrolean Cancer Society.<br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, ROUTINE, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA, MitoKin<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_Omar&diff=226244Torres-Quesada Omar2022-04-11T12:47:54Z<p>Doerrier Carolina: </p>
<hr />
<div>{{EAGLE<br />
|COST=Member<br />
|COST WG4=WG4<br />
}}<br />
{{Person<br />
|lastname=Torres-Quesada<br />
|firstname=Omar<br />
|title=Dr.<br />
|institution=[[File:Torres-QuesadaO.jpg|right|150px|Omar Torres-Quesada ]] Institute of Biochemistry,Center for Chemistry and Biomedicine, University of Innsbruck, AT<br />
|address=Innrain 80-82<br />
|area code=6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress=omar.quesada@uibk.ac.at<br />
|weblink=[https://wiki.oroboros.at/index.php/MitoKIN MitoKIN], [https://www.researchgate.net/profile/Omar-Torres-Quesada ResearchGate profile], ORCID:[[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]]<br />
}}<br />
{{Labelingperson}}<br />
<br />
<br />
<br />
== Participated at ==<br />
::::* [[IOC145|IOC145 Innsbruck AT]]<br />
== FFG project ==<br />
:::: [[MitoKIN]]</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=File:Torres-QuesadaO.jpg&diff=226243File:Torres-QuesadaO.jpg2022-04-11T12:41:21Z<p>Doerrier Carolina: </p>
<hr />
<div></div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Doerrier_Carolina&diff=226232Doerrier Carolina2022-04-11T09:31:15Z<p>Doerrier Carolina: </p>
<hr />
<div>{{EAGLE<br />
|COST= Member<br />
|COST WG1 = WG1<br />
|COST WG2 = WG2<br />
|COST WG3 = WG3<br />
|COST WG4 = WG4<br />
|COST ECI= ECI<br />
}}<br />
: [[Management_Committee_MitoEAGLE#MC_Substitutes|MC Substitute]] - [[Management Committee MitoEAGLE]]<br />
{{NextGen-O2k H2020-support}}<br />
{{Person<br />
|lastname=Doerrier Velasco<br />
|firstname=Carolina<br />
|title=PhD.<br />
|institution=::::::::::::::::[[File:DoerrierC.JPG|right|150px|Carolina Doerrier]]<br />
Scientific Motive Force<br />
<br />
Oroboros Instruments<br />
* Mitochondrial Physiology Specialist ([[O2k-Innovation description|O2k-Innovation]])<br />
* Mitochondrial Application Specialist<br />
* [[NextGen-O2k]]<br />
* [[K-Regio_MitoFit|K-Regio MitoFit]]<br />
<br />
<br />
Background: [[ES Granada Acuna-Castroviejo D]]<br />
<br />
Carolina joined [[Oroboros_Contact |Oroboros Instruments]] in October 2014.<br />
|address=Schoepfstrasse 18<br />
|area code=6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress=carolina.doerrier@oroboros.at<br />
|weblink=[http://www.oroboros.at www.oroboros.at], [[Bioblast|www.bioblast.at]], [http://www.mitophysiology.org www.mitophysiology.org], [https://www.researchgate.net/profile/Carolina_Doerrier_Velasco ResearchGate profile], ORCID:[[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]]<br />
}}<br />
<br />
__TOC__<br />
== Bioenergetics Communications ==<br />
::::* Carolina Doerrier is Section Editor of the journal '''[[Bioenergetics Communications]]'''<br />
:::::'''Keywords:''' O2k-FluoRespirometry, oxidative stress, permeabilized muscle fibers, NAD(P)H, sepsis, aging, melatonin<br />
==[[Oroboros Contact| Oroboros-team]]==<br />
:* Previous laboratory: [[ES Granada Acuna-Castroviejo D]]<br />
:* Collaboration with the [[AT_Innsbruck_Gnaiger E|Gnaiger Lab]] in Innsbruck during a 3-months scholarship (Sep - Dec 2011), in the frame of the K-Regio project ''[[MitoCom_O2k-Fluorometer| MitoCom Tyrol]]''.<br />
:* Collaboration with the [[US FL Orlando Goodpaster BH|Goodpaster Lab]] in Orlando (2017).<br />
:* MitoEAGLE Short-Term Scientific Mission at [[ES Barcelona Garcia-Roves PM]] in Barcelona (2018).<br />
:* Guest tutor at [[IOC65]] and [[IOC68]]. <br />
:* Tutor at [[IOC100]], [[IOC104]], [[IOC106]], [[IOC110]], [[IOC112]], [[IOC114]], [[IOC115]], [[IOC116]], [[IOC120]], [[IOC122]], [[IOC130]], [[IOC132]], [[IOC133]], [[IOC137]], [[IOC139]], [[IOC144]] and [[IOC145]].<br />
:* Current laboratory: [[AT Innsbruck Oroboros]]<br />
== Bioblast Editorial Board ==<br />
<br />
:* Bioblast national editor [[MiPNet Laboratories Spain]]<br />
:* [[Library of protocols]]<br />
<br />
== MitoEAGLE Short-Term Scientific Mission ==<br />
****: [[Short-Term Scientific Missions MitoEAGLE#STSM_Grant_Period_2 |STSM Grant Period 2]]<br />
::: '''Work Plan summary'''<br />
:::: WORKPLAN SUMMARY Different experimental aspects (e.g. purity of the preparations, environmental humidity which could affect the wet weight of the muscle, chemicals) will be evaluated during the STSM in order to clarify the sources of variability found in the MitoEAGLE WG2 pilot study. The preparation of the samples will be performed according the SOP established for the MitoEAGLE WG2 pilot study. Permeabilized fibers from soleus of C57BL6/J mice will be used. Mitochondrial respiration will be assessed by O2k high-resolution respirometry (HRR). The Substrate- Uncoupler-Inhibitor Titration (SUIT) protocol used in the MitoEAGLE WG2 pilot study for obtaining mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers, will be used to perform the experiments during the STSM at the Department of Physiological Sciences II in the Faculty of Medicine (Barcelona University, Barcelona, Spain).<br />
<br />
<br />
<br />
== Participated at ==<br />
::::* [[2020 PaduaMuscleDays Padua IT]]<br />
::::* [[FAT4BRAIN School IOC147 Virtual Event]]<br />
::::* [[MiPNet25.06 IOC145 Innsbruck AT|IOC145 Innsbruck AT]]<br />
::::* [[MiPNet25.03 IOC144 Innsbruck AT|IOC144 Innsbruck AT]]<br />
::::* [[MiP2019/MitoEAGLE Belgrade RS|MitoEAGLE 2019 Belgrade RS]]<br />
::::* [[MiP2019/MitoEAGLE Belgrade RS]]<br />
::::* [[FEBS Workshop Ageing 2019 Innsbruck AT]]<br />
::::* [[Mitochondrial Physiology ‐ from Organelle to Organism 2019 Copenhagen DK]]<br />
::::* [[MiPschool Coimbra 2019|MiP/MitoEAGLE Training School 2019 Coimbra PT]]<br />
::::* [[IOC139| IOC139 Schroecken AT]]<br />
::::* [[Life Science PhD Meeting 2019 Innsbruck AT]]<br />
::::* [[MitoEAGLE Obergurgl 2019-01-31| MitoEAGLE 2019 Obergurgl AT]]<br />
::::* [[IOC137|IOC137 Innsbruck AT]]<br />
::::* [[MitoEAGLE_Innsbruck_2018-11-19| MitoEAGLE 2018 Innsbruck AT]]<br />
::::* [[IOC133|IOC133 Innsbruck AT]]<br />
::::* [[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]]<br />
::::* [[EBEC2018 Budapest HU]]<br />
::::* [[MitoEAGLE Copenhagen 2018| MitoEAGLE 2018 Copenhagen DK]]<br />
::::* [[MiPNet23.06 IOC130 Schroecken AT| IOC130 Schroecken AT]]<br />
::::* [[ESCI 2018 Barcelona ES]]<br />
::::* [[MitoFit Workshop ATP 2017 Innsbruck AT]]<br />
::::* [[MiP2017/MitoEAGLE Hradec Kralove CZ|MitoEAGLE 2017 Hradec Kralove CZ]]<br />
::::* [[MiPNet22.01 IOC122 Schroecken AT]]<br />
::::* [[MitoEAGLE Obergurgl 2017| MitoEAGLE 2017 Obergurgl AT]]<br />
::::* [[MiPschool Obergurgl 2017| MiPschool 2017 Obergurgl AT]]<br />
::::* [[MitoEAGLE Barcelona 2017| MitoEAGLE 2017 Barcelona ES]]<br />
::::* [[MiPNet22.04 IOC120 Barcelona ES | IOC120 Barcelona ES]]<br />
::::* [[Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE| Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE]]<br />
::::* [[MiPNet21.11 IOC116 Innsbruck AT|IOC116 Innsbruck AT]]<br />
::::* [[IOC115 | IOC115 Schroecken AT]]<br />
::::* [[MiPNet21.19 IOC114 Innsbruck AT | IOC114 Innsbruck AT]]<br />
::::* [[MitoEAGLE Verona 2016| MitoEAGLE 2016 Verona IT]]<br />
::::* [[MitoFit Science Camp 2016 Kuehtai AT]]<br />
::::* [[MiPNet21.15 IOC112 Kuehtai AT|IOC112 Kuehtai AT]]<br />
::::* [[MiPNet21.04 IOC110 Melbourne AU|IOC110 Melbourne AU]]<br />
::::* [[Research to Practice 2016 Melbourne AU]]<br />
::::* [[Bioblast 2012 | Bioblast 2012 Innsbruck AT]]<br />
::::* [[MiPNet20.10 IOC106 Schroecken|IOC106 Schroecken AT]]<br />
::::* [[MiPNet20.05 IOC104 Greenville |IOC104 Greenville NC US]]<br />
::::* [[IOC100 | IOC100 Schroecken AT]]<br />
::::* [[IOC68 | IOC68 Schroecken AT]]<br />
::::* [[IOC65 | IOC65 Schroecken AT]]<br />
::::* [[MiPNet14.04 IOC51 | IOC51 Schroecken AT]]<br />
::::* [[MiP2015 | MiP2015 Pec pod Snezkou CZ]]<br />
::::* [[MiPschool Greenville 2015 | MiPschool 2015 Greenville NC US]]<br />
<br />
{{Labelingperson<br />
|field of research=Basic<br />
|topics=[[High-resolution respirometry]], [[Mitochondria]], [[Melatonin]], [[Reactive oxygen species]], [[Reactive nitrogen species]], [[Sepsis]]<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit_Kinase&diff=226134Torres-Quesada 2022 MitoFit Kinase2022-04-08T08:00:54Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada Omar, Strich Sophie, Stefan Eduard (2022) Kinase perturbations redirect mitochondrial function. MitoFit Prep 2022.#. https://doi.org/10.26124/mitofit:2022-####<br />
|info= <br />
|authors=Torres-Quesada Omar, Strich Sophie, Stefan Eduard<br />
|year=2022<br />
|journal=MitoFit Prep<br />
|abstract=Protein kinases take the center stage in numerous signaling pathways by phosphorylating compartmentalized protein substrates for controlling cell proliferation, cell cycle and metabolism. Kinase dysfunctions have been linked to numerous human diseases such as cancer. This has led to the development of kinase inhibitors which aim to target oncogenic kinase activities. The specificity of the cancer blockers depends on the range of targeted kinases. Therefore, the question arises of how cell-type-specific off-target effects impair the specificities of cancer drugs. Blockade of kinase activities has been shown to converge on the energetic organelle, the mitochondria. In this review, we highlight examples of selected major kinases which impact mitochondrial signaling. Further, we discuss pharmacological strategies to target kinase activities which are linked to cancer progression and redirecting mitochondrial function. Finally, we propose that cell-based recordings of mitochondrial bioenergetic states might predict off-target or identify specific on-target effects of kinase inhibitors.<br><br><br />
|keywords=Kinases, signaling, mitochondria, kinase inhibitors, cancer, drug off-target effects <br />
|editor=Doerrier C<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, <br />
[[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
<br />
{{Labeling<br />
|area=Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit_Kinase&diff=226133Torres-Quesada 2022 MitoFit Kinase2022-04-08T07:59:54Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada Omar, Strich Sophie, Stefan Eduard (2022) Kinase perturbations redirect mitochondrial function. MitoFit Prep 2022.#. https://doi.org/10.26124/mitofit:2022-####<br />
|info= <br />
|authors=Torres-Quesada Omar, Strich Sophie, Stefan Eduard<br />
|year=2022<br />
|journal=MitoFit Prep<br />
|abstract=Protein kinases take the center stage in numerous signaling pathways by phosphorylating compartmentalized protein substrates for controlling cell proliferation, cell cycle and metabolism. Kinase dysfunctions have been linked to numerous human diseases such as cancer. This has led to the development of kinase inhibitors which aim to target oncogenic kinase activities. The specificity of the cancer blockers depends on the range of targeted kinases. Therefore, the question arises of how cell-type-specific off-target effects impair the specificities of cancer drugs. Blockade of kinase activities has been shown to converge on the energetic organelle, the mitochondria. In this review, we highlight examples of selected major kinases which impact mitochondrial signaling. Further, we discuss pharmacological strategies to target kinase activities which are linked to cancer progression and redirecting mitochondrial function. Finally, we propose that cell-based recordings of mitochondrial bioenergetic states might predict off-target or identify specific on-target effects of kinase inhibitors.<br />
|keywords=Kinases, signaling, mitochondria, kinase inhibitors, cancer, drug off-target effects<br />
|editor=Doerrier C<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, <br />
[[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
<br />
{{Labeling<br />
|area=Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit_Kinase&diff=226132Torres-Quesada 2022 MitoFit Kinase2022-04-08T07:56:07Z<p>Doerrier Carolina: Created page with "{{Publication |title=Torres-Quesada Omar, Strich Sophie, Stefan Eduard (2022) Kinase perturbations redirect mitochondrial function. MitoFit Prep 2022.#. |authors=Torres-Quesad..."</p>
<hr />
<div>{{Publication<br />
|title=Torres-Quesada Omar, Strich Sophie, Stefan Eduard (2022) Kinase perturbations redirect mitochondrial function. MitoFit Prep 2022.#.<br />
|authors=Torres-Quesada Omar, Strich Sophie, Stefan Eduard<br />
|year=2022<br />
|journal=MitoFit Prep<br />
|abstract=Protein kinases take the center stage in numerous signaling pathways by phosphorylating compartmentalized protein substrates for controlling cell proliferation, cell cycle and metabolism. Kinase dysfunctions have been linked to numerous human diseases such as cancer. This has led to the development of kinase inhibitors which aim to target oncogenic kinase activities. The specificity of the cancer blockers depends on the range of targeted kinases. Therefore, the question arises of how cell-type-specific off-target effects impair the specificities of cancer drugs. Blockade of kinase activities has been shown to converge on the energetic organelle, the mitochondria. In this review, we highlight examples of selected major kinases which impact mitochondrial signaling. Further, we discuss pharmacological strategies to target kinase activities which are linked to cancer progression and redirecting mitochondrial function. Finally, we propose that cell-based recordings of mitochondrial bioenergetic states might predict off-target or identify specific on-target effects of kinase inhibitors.<br />
|keywords=Kinases, signaling, mitochondria, kinase inhibitors, cancer, drug off-target effects<br />
|editor=Doerrier C<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
{{Labeling<br />
|area=Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit&diff=225985Torres-Quesada 2022 MitoFit2022-04-04T12:47:00Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines. MitoFit Preprints 2022.8. https://doi.org/10.26124/mitofit:2022-0008<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines]<br/><br />
|authors=Torres-Quesada Omar, Doerrier Carolina, Strich Sophie, Gnaiger Erich, Stefan Eduard<br />
|year=2022-04-01<br />
|journal=MitoFit Prep<br />
|abstract=Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. The simplicity of these models facilitates the reproducible manipulation of cultured cells. Limitations, however, relate to cell growth in a monolayer using standard cell culture media. Although such media are in use for decades, their formulations do not mimic the composition of the originating human cell environment in physiological or pathological conditions. In this study, we selected three frequently-used cancer cell lines SW620 (colon cancer), MCF7 (breast cancer), and A375 (melanoma) to determine the impact of a newly formulated human plasma-like medium (HPLM) on cell proliferation and mitochondrial function. Mitochondrial bioenergetic profiles were analyzed by high-resolution respirometry, revealing metabolic reprogramming in cells cultured in standard and HPLM media. Notably, the culturing of differentially proliferating cells in HPLM affected key mechanism of cellular respiration. All three cancer cell lines displayed an amending bioenergetic profile, particularly related to mitochondrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinically prescribed cancer drug and kinase inhibitor sunitinib. These findings highlight that cell culture media redirect mitochondrial function and affect cancer drug sensitivities. To advance the translational potential of cell culture models we propose to prioritize media with a human plasma-like composition for analyzing bioenergetic profiles and for determining target-oriented drug efficacies. <br><br><br />
|keywords=Cancer cells, cell respiration, mitochondrial function, HPLM, classical media, metabolism, cell culture media, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residual O2 consumption, bioenergetic cluster analysis BCA<br />
|editor=Tindle-Solomon L<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
__TOC__<br />
== Data availability ==<br />
:::: Original files are available Open Access at Zenodo repository: https://doi.org/10.5281/zenodo.6402435<br />
<br />
== Support ==<br />
:::: This work was founded by the projects 877163 for the Austrian FFG-Bridge program [[MitoKIN]], the Austrian Science Fund (FWF; P27606, P30441, P32960, P35159) and the Tyrolean Cancer Society.<br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, ROUTINE, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit&diff=225877Torres-Quesada 2022 MitoFit2022-04-01T08:54:58Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models. MitoFit Preprints 2022.8. https://doi.org/10.26124/mitofit:2022-0008<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models]<br/><br />
|authors=Torres-Quesada Omar, Doerrier Carolina, Strich Sophie, Gnaiger Erich, Stefan Eduard<br />
|year=2022-04-01<br />
|journal=MitoFit Prep<br />
|abstract=Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. The simplicity of these models facilitates the reproducible manipulation of cultured cells. Limitations, however, relate to cell growth in a monolayer using standard cell culture media. Although such media are in use for decades, their formulations do not mimic the composition of the originating human cell environment in physiological or pathological conditions. In this study, we selected three frequently-used cancer cell lines SW620 (colon cancer), MCF7 (breast cancer), and A375 (melanoma) to determine the impact of a newly formulated human plasma-like medium (HPLM) on cell proliferation and mitochondrial function. Mitochondrial bioenergetic profiles were analyzed by high-resolution respirometry, revealing metabolic reprogramming in cells cultured in standard and HPLM media. Notably, the culturing of differentially proliferating cells in HPLM affected key mechanism of cellular respiration. All three cancer cell lines displayed an amending bioenergetic profile, particularly related to mitochondrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinically prescribed cancer drug and kinase inhibitor sunitinib. These findings highlight that cell culture media redirect mitochondrial function and affect cancer drug sensitivities. To advance the translational potential of cell culture models we propose to prioritize media with a human plasma-like composition for analyzing bioenergetic profiles and for determining target-oriented drug efficacies. <br><br><br />
|keywords=Cancer cells, cell respiration, mitochondrial function, HPLM, classical media, metabolism, cell culture media, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residual O2 consumption, bioenergetic cluster analysis BCA<br />
|editor=Tindle-Solomon L<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
__TOC__<br />
== Data availability ==<br />
:::: Original files are available Open Access at Zenodo repository: https://doi.org/10.5281/zenodo.6402435<br />
<br />
== Support ==<br />
:::: This work was founded by the projects 877163 for the Austrian FFG-Bridge program [[MitoKIN]], the Austrian Science Fund (FWF; P27606, P30441, P32960, P35159) and the Tyrolean Cancer Society.<br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, ROUTINE, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit&diff=225876Torres-Quesada 2022 MitoFit2022-04-01T08:51:14Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models. MitoFit Preprints 2022.8. https://doi.org/10.26124/mitofit:2022-0008<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/1/16/Torres-Quesada_2022_MitoFitPreprints.pdf Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models]<br/><br />
|authors=Torres-Quesada Omar, Doerrier Carolina, Strich Sophie, Gnaiger Erich, Stefan Eduard<br />
|year=2022-04-01<br />
|journal=MitoFit Prep<br />
|abstract=Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. The simplicity of these models facilitates the reproducible manipulation of cultured cells. Limitations, however, relate to cell growth in a monolayer using standard cell culture media. Although such media are in use for decades, their formulations do not mimic the composition of the originating human cell environment in physiological or pathological conditions. In this study, we selected three frequently-used cancer cell lines SW620 (colon cancer), MCF7 (breast cancer), and A375 (melanoma) to determine the impact of a newly formulated human plasma-like medium (HPLM) on cell proliferation and mitochondrial function. Mitochondrial bioenergetic profiles were analyzed by high-resolution respirometry, revealing metabolic reprogramming in cells cultured in standard and HPLM media. Notably, the culturing of differentially proliferating cells in HPLM affected key mechanism of cellular respiration. All three cancer cell lines displayed an amending bioenergetic profile, particularly related to mitochondrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinically prescribed cancer drug and kinase inhibitor sunitinib. These findings highlight that cell culture media redirect mitochondrial function and affect cancer drug sensitivities. To advance the translational potential of cell culture models we propose to prioritize media with a human plasma-like composition for analyzing bioenergetic profiles and for determining target-oriented drug efficacies. <br><br><br />
|keywords=Cancer cells, cell respiration, mitochondrial function, HPLM, classical media, metabolism, cell culture media, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residual O2 consumption, bioenergetic cluster analysis BCA<br />
|editor=Tindle-Solomon L<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
__TOC__<br />
== Data availability ==<br />
:::: Original files are available Open Access at Zenodo repository: (in preparation)<br />
<br />
== Support ==<br />
:::: This work was founded by the projects 877163 for the Austrian FFG-Bridge program [[MitoKIN]], the Austrian Science Fund (FWF; P27606, P30441, P32960, P35159) and the Tyrolean Cancer Society.<br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=LEAK, ROUTINE, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit&diff=225869Torres-Quesada 2022 MitoFit2022-04-01T06:48:45Z<p>Doerrier Carolina: </p>
<hr />
<div>In prep<br />
<br />
{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models. MitoFit Preprints 2022.8. https://doi.org/10.26124/mitofit:2022-0008<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=###|MitoFit pdf]] [### Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models]<br/><br />
<br />
|authors=Torres-Quesada Omar, Doerrier Carolina, Strich Sophie, Gnaiger Erich, Stefan Eduard<br />
<br />
|year=2022-03-31<br />
<br />
|journal=MitoFit Prep<br />
|abstract= Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. The simplicity of these models facilitates the reproducible manipulation of cultured cells. Limitations, however, relate to cell growth in a monolayer using standard cell culture media. Although such media are in use for decades, their formulations do not mimic the composition of the originating human cell environment in physiological or pathological conditions. In this study, we selected three frequently-used cancer cell lines SW620 (colon cancer), MCF7 (breast cancer), and A375 (melanoma) to determine the impact of a newly formulated human plasma-like medium (HPLM) on cell proliferation and mitochondrial function. Mitochondrial bioenergetic profiles were analyzed by high-resolution respirometry, revealing metabolic reprogramming in cells cultured in standard and HPLM media. Notably, the culturing of differentially proliferating cells in HPLM affected key mechanism of cellular respiration. All three cancer cell lines displayed an amending bioenergetic profile, particularly related to mitochondrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinically prescribed cancer drug and kinase inhibitor sunitinib. These findings highlight that cell culture media redirect mitochondrial function and affect cancer drug sensitivities. To advance the translational potential of cell culture models we propose to prioritize media with a human plasma-like composition for analyzing bioenergetic profiles and for determining target-oriented drug efficacies. <br><br><br />
<br />
|keywords=Cancer cells, cell respiration, mitochondrial function, HPLM, classical media, metabolism, cell culture media, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residual O2 consumption, bioenergetic cluster analysis BCA|editor=Tindle-Solomon L<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
__TOC__<br />
== Data availability ==<br />
:::: Original files are available Open Access at Zenodo repository: (in preparation)<br />
<br />
== Support ==<br />
:::: This work was founded by the projects 877163 for the Austrian FFG-Bridge program [[MitoKIN]], the Austrian Science Fund (FWF; P27606, P30441, P32960, P35159) and the Tyrolean Cancer Society. <br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=ROUTINE, LEAK, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit&diff=225866Torres-Quesada 2022 MitoFit2022-04-01T05:46:45Z<p>Doerrier Carolina: </p>
<hr />
<div>In prep<br />
<br />
{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models. MitoFit Preprints 2022.8. https://doi.org/10.26124/mitofit:2022-0008<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=###|MitoFit pdf]] [### Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models]<br/><br />
<br />
|authors=Torres-Quesada Omar, Doerrier Carolina, Strich Sophie, Gnaiger Erich, Stefan Eduard<br />
<br />
|year=2022-03-31<br />
<br />
|journal=MitoFit Prep<br />
|abstract= Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. The simplicity of these models facilitates the reproducible manipulation of cultured cells. Limitations, however, relate to cell growth in a monolayer using standard cell culture media. Although such media are in use for decades, their formulations do not mimic the composition of the originating human cell environment in physiological or pathological conditions. In this study, we selected three frequently-used cancer cell lines SW620 (colon cancer), MCF7 (breast cancer), and A375 (melanoma) to determine the impact of a newly formulated human plasma-like medium (HPLM) on cell proliferation and mitochondrial function. Mitochondrial bioenergetic profiles were analyzed by high-resolution respirometry, revealing metabolic reprogramming in cells cultured in standard and HPLM media. Notably, the culturing of differentially proliferating cells in HPLM affected key mechanism of cellular respiration. All three cancer cell lines displayed an amending bioenergetic profile, particularly related to mitochondrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinically prescribed cancer drug and kinase inhibitor sunitinib. These findings highlight that cell culture media redirect mitochondrial function and affect cancer drug sensitivities. To advance the translational potential of cell culture models we propose to prioritize media with a human plasma-like composition for analyzing bioenergetic profiles and for determining target-oriented drug efficacies. <br><br><br />
<br />
|keywords=Cancer cells, cell respiration, mitochondrial function, HPLM, classical media, metabolism, cell culture media, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residual O2 consumption, bioenergetic cluster analysis BCA|editor=Tindle-Solomon L<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
<br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=ROUTINE, LEAK, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit&diff=225865Torres-Quesada 2022 MitoFit2022-04-01T05:42:49Z<p>Doerrier Carolina: </p>
<hr />
<div>In prep<br />
<br />
{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models. MitoFit Preprints 2022.8. https://doi.org/10.26124/mitofit:2022-0008<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=###|MitoFit pdf]] [### Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models]<br/><br />
<br />
|authors=Torres-Quesada Omar, Doerrier Carolina, Strich Sophie, Gnaiger Erich, Stefan Eduard<br />
<br />
|year=2022-03-31<br />
<br />
|journal=MitoFit Prep<br />
|abstract= Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. The simplicity of these models facilitates the reproducible manipulation of cultured cells. Limitations, however, relate to cell growth in a monolayer using standard cell culture media. Although such media are in use for decades, their formulations do not mimic the composition of the originating human cell environment in physiological or pathological conditions. In this study, we selected three frequently-used cancer cell lines SW620 (colon cancer), MCF7 (breast cancer), and A375 (melanoma) to determine the impact of a newly formulated human plasma-like medium (HPLM) on cell proliferation and mitochondrial function. Mitochondrial bioenergetic profiles were analyzed by high-resolution respirometry, revealing metabolic reprogramming in cells cultured in standard and HPLM media. Notably, the culturing of differentially proliferating cells in HPLM affected key mechanism of cellular respiration. All three cancer cell lines displayed an amending bioenergetic profile, particularly related to mitochondrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinically prescribed cancer drug and kinase inhibitor sunitinib. These findings highlight that cell culture media redirect mitochondrial function and affect cancer drug sensitivities. To advance the translational potential of cell culture models we propose to prioritize media with a human plasma-like composition for analyzing bioenergetic profiles and for determining target-oriented drug efficacies. <br><br><br />
<br />
|keywords=Cancer cells, cell respiration, mitochondrial function, HPLM, classical media, metabolism, cell culture media, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residual O2 consumption, bioenergetic cluster analysis BCA|editor=Tindle-Solomon L<br />
<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
<br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=ROUTINE, LEAK, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, colon cancer cells, breast cancer cells, melanoma, BCA<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Torres-Quesada_2022_MitoFit&diff=225864Torres-Quesada 2022 MitoFit2022-04-01T05:40:45Z<p>Doerrier Carolina: </p>
<hr />
<div>In prep<br />
<br />
{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models. MitoFit Preprints 2022.8. https://doi.org/10.26124/mitofit:2022-0008<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=###|MitoFit pdf]] [### Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell culture models]<br/><br />
<br />
|authors=Torres-Quesada Omar, Doerrier Carolina, Strich Sophie, Gnaiger Erich, Stefan Eduard<br />
<br />
|year=2022-03-31<br />
<br />
|journal=MitoFit Prep<br />
|abstract= Two-dimensional cell cultures are established models in research for studying and perturbing cell-type specific functions. The simplicity of these models facilitates the reproducible manipulation of cultured cells. Limitations, however, relate to cell growth in a monolayer using standard cell culture media. Although such media are in use for decades, their formulations do not mimic the composition of the originating human cell environment in physiological or pathological conditions. In this study, we selected three frequently-used cancer cell lines SW620 (colon cancer), MCF7 (breast cancer), and A375 (melanoma) to determine the impact of a newly formulated human plasma-like medium (HPLM) on cell proliferation and mitochondrial function. Mitochondrial bioenergetic profiles were analyzed by high-resolution respirometry, revealing metabolic reprogramming in cells cultured in standard and HPLM media. Notably, the culturing of differentially proliferating cells in HPLM affected key mechanism of cellular respiration. All three cancer cell lines displayed an amending bioenergetic profile, particularly related to mitochondrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinically prescribed cancer drug and kinase inhibitor sunitinib. These findings highlight that cell culture media redirect mitochondrial function and affect cancer drug sensitivities. To advance the translational potential of cell culture models we propose to prioritize media with a human plasma-like composition for analyzing bioenergetic profiles and for determining target-oriented drug efficacies. <br><br><br />
<br />
|keywords=Cancer cells, cell respiration, mitochondrial function, HPLM, classical media, metabolism, cell culture media, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK respiration, electron transfer capacity, residual O2 consumption, bioenergetic cluster analysis BCA|editor=Tindle-Solomon L<br />
<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3394-3075]] Torres-Quesada Omar, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3650-4713]] Stefan Eduard<br />
<br />
<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|diseases=Cancer<br />
|organism=Human<br />
|tissues=Other cell lines<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling<br />
|couplingstates=ROUTINE, LEAK, ET<br />
|pathways=ROX<br />
|instruments=Oxygraph-2k<br />
|additional=SUIT-003, BCA<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Balbaisi_2022_MitoFit&diff=225285Balbaisi 2022 MitoFit2022-03-09T16:03:57Z<p>Doerrier Carolina: </p>
<hr />
<div> In prep<br />
<br />
{{MitoFit page name}}<br />
{{Publication<br />
|title=Balbaisi A, Stiban J (2022) Barth Syndrome: A Genetic Ailment with a Lipid Component and Bioenergetic Ramifications. MitoFit Preprints 2022.3. [[doi:10.26124/mitofit:2022-0003]]<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=|MitoFit pdf]] [https://wiki.oroboros.at/images/2/23/ Barth Syndrome: A Genetic Ailment with a Lipid Component and Bioenergetic Ramifications]<br/><br />
|authors=Balbaisi Abdallah , Stiban Johnny<br />
|year=2022-03-09<br />
|journal=MitoFit Prep<br />
|abstract=<br />
In eukaryotes membranes are structural components that are necessary for compartmentalization of function. Membranes consist of a lipid bilayer with a multitude of proteins on or in this sandwich. Nevertheless, membranes are not solely structural in function but also, they serve as basis for cellular signaling and metabolism. Membranes vary with respect to their lipid composition, protein:lipid ratio, thickness, carbohydrate content, etc., and hence their functions are not necessarily identical in the different compartments. In the mitochondrial inner membrane (mtIM), as in its bacterial ancestor, a special phospholipid is present. Cardiolipin (CL) is a phospholipid consisting of four hydrophobic tails. It is essential for the assembly of the electron transport system (ETS) and its components, and hence CL is required for efficient mitochondrial bioenergetics. Mutations in CL remodeling enzyme encoded by the tafazzin gene (''TAZ'') are associated with a syndrome first identified by Dutch scientist Peter Barth, hence the name Barth Syndrome. Here, we review recent research on this devastating syndrome focusing on CL biosynthesis and remodeling and relationship between the phospholipid component and mitochondrial bioenergetics. We further by exploring management and possible future techniques in the treatment of this syndrome. <br><br><br />
<br />
|keywords=Barth syndrome, tafazzin, cardiolipin, cardiolipin remodeling, 3-MGA, respiratory complexes<br />
|editor=Tindle-Solomon L<br />
|mipnetlab=<br />
}}<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-3748-9976]] Stiban Johnny<br />
<br />
{{Labeling<br />
|area=mt-Membrane <br />
|diseases=Inherited<br />
|organism=Human<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Balbaisi_2022_MitoFit&diff=225284Balbaisi 2022 MitoFit2022-03-09T16:02:42Z<p>Doerrier Carolina: </p>
<hr />
<div> In prep<br />
<br />
{{MitoFit page name}}<br />
{{Publication<br />
|title=Balbaisi A, Stiban J (2022) Barth Syndrome: A Genetic Ailment with a Lipid Component and Bioenergetic Ramifications. MitoFit Preprints 2022.3. [[doi:10.26124/mitofit:2022-0003]]<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link=|MitoFit pdf]] [https://wiki.oroboros.at/images/2/23/ Barth Syndrome: A Genetic Ailment with a Lipid Component and Bioenergetic Ramifications]<br/><br />
|authors=Balbaisi Abdallah , Stiban Johnny<br />
|year=2022-03-09<br />
|journal=MitoFit Prep<br />
|abstract=<br />
In eukaryotes membranes are structural components that are necessary for compartmentalization of function. Membranes consist of a lipid bilayer with a multitude of proteins on or in this sandwich. Nevertheless, membranes are not solely structural in function but also, they serve as basis for cellular signaling and metabolism. Membranes vary with respect to their lipid composition, protein:lipid ratio, thickness, carbohydrate content, etc., and hence their functions are not necessarily identical in the different compartments. In the mitochondrial inner membrane (mtIM), as in its bacterial ancestor, a special phospholipid is present. Cardiolipin (CL) is a phospholipid consisting of four hydrophobic tails. It is essential for the assembly of the electron transport system (ETS) and its components, and hence CL is required for efficient mitochondrial bioenergetics. Mutations in CL remodeling enzyme encoded by the tafazzin gene (''TAZ'') are associated with a syndrome first identified by Dutch scientist Peter Barth, hence the name Barth Syndrome. Here, we review recent research on this devastating syndrome focusing on CL biosynthesis and remodeling and relationship between the phospholipid component and mitochondrial bioenergetics. We further by exploring management and possible future techniques in the treatment of this syndrome. <br><br><br />
<br />
|keywords=Barth syndrome, tafazzin, cardiolipin, cardiolipin remodeling, 3-MGA, respiratory complexes<br />
|editor=Tindle-Solomon L<br />
|mipnetlab=<br />
}}<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-3748-9976]] Stiban Johnny<br />
<br />
{{Labeling<br />
|diseases=Inherited<br />
|organism=Human<br />
}}</div>Doerrier Carolinahttps://wiki.oroboros.at/index.php?title=Fischer_2022_MitoFit_Fe&diff=225052Fischer 2022 MitoFit Fe2022-03-03T12:57:43Z<p>Doerrier Carolina: </p>
<hr />
<div>{{MitoFit page name}}<br />
<br />
{{Publication<br />
|title=Fischer C, Valente de Souza L, Komlódi T, Garcia-Souza LF, Volani C, Tymoszuk P, Demetz E, Seifert M, Auer K, Hilbe R, Brigo N, Petzer V, Asshoff M, Gnaiger E, Weiss G (2022) Mitochondrial respiration in response to iron deficiency anemia. Comparison of peripheral blood mononuclear cells and liver. MitoFit Preprints 2022.2. [[doi:10.26124/mitofit:2022-0002]]<br />
|info=[[File:MitoFit Preprints pdf.png|left|160px|link= |MitoFit pdf]] [Mitochondrial respiration in response to iron deficiency anemia. Comparison of peripheral blood mononuclear cells and liver]<br/><br />
|authors=Fischer Christine, Valente de Souza Lara, Komlódi Timea, Garcia-Souza Luiz F, Volani Chiara, Tymoszuk Piotr, Demetz Egon, Seifert Markus, Auer Kristina, Hilbe Richard, Brigo Natascha, Petzer Verena, Asshoff Malte, Gnaiger Erich, Weiss Guenter<br />
|year=2022-03-03<br />
|journal=MitoFit Prep<br />
|abstract=Iron is an essential component for metabolic processes including oxygen transport within hemoglobin, tricarboxylic acid (TCA) cycle activity and mitochondrial energy transformation. Iron deficiency can thus lead to metabolic dysfunction and eventually result in iron deficiency anemia (IDA) which affects approximately 1.5 billion people worldwide. Using a rat model of IDA induced by phlebotomy, we studied the effects of IDA on mito-chondrial respiration in peripheral blood mononuclear cells (PBMCs) and liver. Furthermore, we evaluated whether mitochondrial function evaluated by high-resolution respirometry in PBMCs reflects corresponding alterations in the liver. Surprisingly, mitochondrial respiratory capacity was increased in PBMCs from rats with IDA compared to controls. In contrast, mitochondrial respiration remained unaffected in livers from IDA rats. Of note, citrate synthase activity indicated an increased mitochondrial density in PBMCs, whereas it remained unchanged in the liver, partly explaining the different responses of mitochondrial respiration in PBMCs and liver. Taken together, these results indicate that mitochondrial function determined in PBMCs cannot serve as a valid surrogate for respiration in the liver. Metabolic adaptions to iron deficiency resulted in different metabolic reprogramming in the blood cells and liver tissue.<br />
<br><br><br />
<br />
|keywords=anemia, iron deficiency, peripheral blood mononuclear cells, liver, mitochondrial function, OXPHOS, mitochondrial respiration, surrogate<br />
|editor=Doerrier C<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-5656-5030]] Fischer Christine, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-9876-1411]] Komlódi Timea, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-1474-5428]] Garcia-Souza Luiz F, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3600-4735]] Volani Chiara, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-0398-6034]] Tymoszuk Piotr, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-5723-927X]] Demetz Egon, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-0709-2158]] Weiss Guenter<br />
__TOC__<br />
== Data availability ==<br />
:::: Original files are not provided by the authors.<br />
<br />
== Support ==<br />
:::: This research was funded by the Christian Doppler Laboratory for Iron Metabolism and Anemia Research, the FWF funded doctoral program HOROS (W-1253, to GW) and the transnational doctoral program BI-DOC between the Medical University of Innsbruck, Austria and the Institute of Biomedicine, Eurac, in Bolzano, Italy.<br />
<br />
{{Labeling<br />
|area=Respiration<br />
|diseases=Other<br />
|organism=Rat<br />
|tissues=Liver, Blood cells<br />
|preparations=Permeabilized cells, Homogenate<br />
|enzymes=Marker enzyme<br />
|couplingstates=LEAK, ROUTINE, OXPHOS, ET<br />
|pathways=N, S, NS, Other combinations, ROX<br />
|instruments=Oxygraph-2k<br />
}}</div>Doerrier Carolina