Uhlen 2015 Science
| Uhlén M1, Fagerberg L2, Hallström BM3, Lindskog C4, Oksvold P2, Mardinoglu A5, Sivertsson Å2, Kampf C4, Sjöstedt E6, Asplund A4, Olsson I4, Edlund K7, Lundberg E2, Navani S8, Szigyarto CA9, Odeberg J2, Djureinovic D4, Takanen JO9, Hober S9, Alm T2, Edqvist PH4, Berling H9, Tegel H9, Mulder J10, Rockberg J9, Nilsson P2, Schwenk JM2, Hamsten M9, von Feilitzen K2, Forsberg M2, Persson L2, Johansson F2, Zwahlen M2, von Heijne G11, Nielsen J12, Pontén F4. (2015) Proteomics. Tissue-based map of the human proteome. Science 347:1260419. |
Uhlen M, Fagerberg L, Hallstroem BM, Lindskog C, Oksvold P, Mardinoglu A, Sivertsson Aa, Kampf C, Sjoestedt E, Asplund A, Olsson I, Edlund K, Lundberg E, Navani S, Szigyarto CA, Odeberg J, Djureinovic D, Takanen JO, Hober S, Alm T, Edqvist PH, Berling H, Tegel H, Mulder J, Rockberg J, Nilsson P, Schwenk JM, Hamsten M, von Feilitzen K, Forsberg M, Persson L, Johansson F, Zwahlen M, von Heijne G, Nielsen J, Ponten F (2015) Science
Abstract: Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.
Labels: MiParea: nDNA;cell genetics
Organism: Human
