St-Pierre 2000 Am J Physiol Regul Integr Comp Physiol: Difference between revisions
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|area=Respiration, Comparative MiP;environmental MiP | |||
|taxonomic group=Amphibians | |taxonomic group=Amphibians | ||
|tissues=Skeletal muscle | |||
|preparations=Isolated mitochondria | |preparations=Isolated mitochondria | ||
|injuries=Ischemia-reperfusion | |injuries=Ischemia-reperfusion, Temperature | ||
|topics=O2, Oxygen | |||
|couplingstates=LEAK, OXPHOS | |||
|substratestates=CI | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|articletype=Protocol; Manual | |articletype=Protocol; Manual | ||
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Revision as of 11:00, 7 October 2015
| St-Pierre J, Tattersall GJ, Boutilier RG (2000) Metabolic depression and enhanced O2 affinity of mitochondria in hypoxic hypometabolism. Am J Physiol Regul Integr Comp Physiol 279:R1205-14. |
St-Pierre J, Tattersall GJ, Boutilier RG (2000) Am J Physiol Regul Integr Comp Physiol
Abstract: This study examined whether the steady-state hypometabolism seen in overwintering frogs (Rana temporaria) is reflected at the mitochondrial level either by a reduction in their resting (state 4) and active (state 3) respiration rates and/or by increases in O2 affinity. We isolated mitochondria from the skeletal muscle of cold-submerged frogs at different stages during their hibernation in normoxic and hypoxic water. A modest metabolic depression at the whole animal level (normoxic submergence) was not associated with a reduction in mitochondrial state 4 and state 3 respiration rates. However, mitochondria isolated from frogs that were submerged for 1 mo manifested an increase in their O2 affinity compared with controls and with animals submerged for 4 mo. Hypometabolism was more pronounced at the whole animal level during hypoxic submergence and was accompanied by 1) a reduction in mitochondrial state 4 and state 3 rates and 2) an increase in the O2 affinity of mitochondria. These findings demonstrate that metabolic depression can be reflected at all levels of biological organization in hypoxia-tolerant animals. • Keywords: Frog, Hypoxia, State 3, State 4, P50
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Stress:Ischemia-reperfusion, Temperature
Tissue;cell: Skeletal muscle Preparation: Isolated mitochondria
Regulation: O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Oxygen"Oxygen" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: LEAK, OXPHOS
HRR: Oxygraph-2k
