St-Pierre 2000 Am J Physiol Regul Integr Comp Physiol: Difference between revisions

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{{Publication
{{Publication
|title=St-Pierre J, Tattersall GJ, Boutilier RG (2000) Metabolic depression and enhanced O2 affinity of mitochondria in hypoxic hypometabolism. Am J Physiol Regul Integr Comp Physiol 279:R1205-14.
|title=St-Pierre J, Tattersall GJ, Boutilier RG (2000) Metabolic depression and enhanced O2 affinity of mitochondria in hypoxic hypometabolism. Am J Physiol Regul Integr Comp Physiol 279:R1205-14.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/11003985 PMID: 11003985]
|info=[http://www.ncbi.nlm.nih.gov/pubmed/11003985 PMID: 11003985 Open Access]
|authors=St-Pierre J, Tattersall GJ, Boutilier RG
|authors=St-Pierre J, Tattersall GJ, Boutilier RG
|year=2000
|year=2000
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|injuries=Hypoxia
|injuries=Hypoxia
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|additional=Labels
|articletype=Protocol; Manual
|articletype=Protocol; Manual
}}
}}

Revision as of 12:38, 26 March 2015

Publications in the MiPMap
St-Pierre J, Tattersall GJ, Boutilier RG (2000) Metabolic depression and enhanced O2 affinity of mitochondria in hypoxic hypometabolism. Am J Physiol Regul Integr Comp Physiol 279:R1205-14.

ยป PMID: 11003985 Open Access

St-Pierre J, Tattersall GJ, Boutilier RG (2000) Am J Physiol Regul Integr Comp Physiol

Abstract: This study examined whether the steady-state hypometabolism seen in overwintering frogs (Rana temporaria) is reflected at the mitochondrial level either by a reduction in their resting (state 4) and active (state 3) respiration rates and/or by increases in O2 affinity. We isolated mitochondria from the skeletal muscle of cold-submerged frogs at different stages during their hibernation in normoxic and hypoxic water. A modest metabolic depression at the whole animal level (normoxic submergence) was not associated with a reduction in mitochondrial state 4 and state 3 respiration rates. However, mitochondria isolated from frogs that were submerged for 1 mo manifested an increase in their O2 affinity compared with controls and with animals submerged for 4 mo. Hypometabolism was more pronounced at the whole animal level during hypoxic submergence and was accompanied by 1) a reduction in mitochondrial state 4 and state 3 rates and 2) an increase in the O2 affinity of mitochondria. These findings demonstrate that metabolic depression can be reflected at all levels of biological organization in hypoxia-tolerant animals. โ€ข Keywords: Frog, Hypoxia, State 3, State 4, P50


Labels:

Stress:Hypoxia 


Preparation: Isolated mitochondria 



HRR: Oxygraph-2k 

Labels 

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