St-Pierre 2000 Am J Physiol Regul Integr Comp Physiol: Difference between revisions
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|title=St-Pierre J, Tattersall GJ, Boutilier RG (2000) Metabolic depression and enhanced O2 affinity of mitochondria in hypoxic hypometabolism. Am J Physiol Regul Integr Comp Physiol 279:R1205-14. | |title=St-Pierre J, Tattersall GJ, Boutilier RG (2000) Metabolic depression and enhanced O2 affinity of mitochondria in hypoxic hypometabolism. Am J Physiol Regul Integr Comp Physiol 279:R1205-14. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/11003985 PMID: 11003985] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/11003985 PMID: 11003985 Open Access] | ||
|authors=St-Pierre J, Tattersall GJ, Boutilier RG | |authors=St-Pierre J, Tattersall GJ, Boutilier RG | ||
|year=2000 | |year=2000 | ||
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|injuries=Hypoxia | |injuries=Hypoxia | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=Labels | |||
|articletype=Protocol; Manual | |articletype=Protocol; Manual | ||
}} | }} |
Revision as of 12:38, 26 March 2015
St-Pierre J, Tattersall GJ, Boutilier RG (2000) Metabolic depression and enhanced O2 affinity of mitochondria in hypoxic hypometabolism. Am J Physiol Regul Integr Comp Physiol 279:R1205-14. |
St-Pierre J, Tattersall GJ, Boutilier RG (2000) Am J Physiol Regul Integr Comp Physiol
Abstract: This study examined whether the steady-state hypometabolism seen in overwintering frogs (Rana temporaria) is reflected at the mitochondrial level either by a reduction in their resting (state 4) and active (state 3) respiration rates and/or by increases in O2 affinity. We isolated mitochondria from the skeletal muscle of cold-submerged frogs at different stages during their hibernation in normoxic and hypoxic water. A modest metabolic depression at the whole animal level (normoxic submergence) was not associated with a reduction in mitochondrial state 4 and state 3 respiration rates. However, mitochondria isolated from frogs that were submerged for 1 mo manifested an increase in their O2 affinity compared with controls and with animals submerged for 4 mo. Hypometabolism was more pronounced at the whole animal level during hypoxic submergence and was accompanied by 1) a reduction in mitochondrial state 4 and state 3 rates and 2) an increase in the O2 affinity of mitochondria. These findings demonstrate that metabolic depression can be reflected at all levels of biological organization in hypoxia-tolerant animals. โข Keywords: Frog, Hypoxia, State 3, State 4, P50
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Stress:Hypoxia
Preparation: Isolated mitochondria
HRR: Oxygraph-2k
Labels