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- Chemical background + ([[File:Chb.png|100px|https://wiki.oroboros … [[File:Chb.png|100px|https://wiki.oroboros.at/index.php/File:Chb.png]] '''Chemical background''' ''Chb'' is due to autooxidation of the reagents. During CIV assays, ascorbate and TMPD are added to maintain cytochrome ''c'' in a reduced state. External cytochrome ''c'' may be included in the CIV assay. The autooxidation of these compounds is linearly oxygen-dependent down to approximately 50 µM oxygen and responsible for the chemical background oxygen flux after the inhibition of CIV. Oxygen flux due to the chemical reaction of autooxidation must be corrected for the [[Oxygen flux - instrumental background|instrumental O2 background]]. The correction for chemical background is necessary to determine CIV activity, in which case the instrumental O2 background and chemical background may be combined in an overall correction term.be combined in an overall correction term.)
