Search results

|title=Capaldi RA (1973) On the subunit structure of oligomycin sensitive ATPase. Biochem Biophys Res Commun 53:1331-7. |abstract=The subunit structure of oligomycin sensitive ATPase has been determined. In addition to the components of F1, and the so-called

== [[SUITbrowser]] question: Unspecific ATPase activity == = List of publications: ATPase =

...in-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Physiol Biochem Zool 80:422-32. ...associated with maintaining these diverse proton gradients requires that V-ATPase activity be downregulated under anoxia in order to attain the almost comple

...conformational plasticity of the intact ''Thermus thermophilus'' V/A-type ATPase. Science 365:eaaw9144. https://doi.org/10.1126/science.aaw9144 ...ission, and proton translocation, which are relevant for the entire V-type ATPase family.

...liarani A (2016) Preferential nitrite inhibition of the mitochondrial F1FO-ATPase activities when activated by Ca(2+) in replacement of the natural cofactor ...1FO-ATPase activity activated by Ca²⁺, henceforth defined as Ca-ATPase(s), or by the natural cofactor Mg²⁺, was investigated by evaluat

...rified from bakers' yeast mitochondria. The enzyme resembled mitochondrial ATPase from beef heart with respect to substrate specificity, cold lability, and o ...idative phosphorylation in submitochondrial yeast particles. Mitochondrial ATPase from beef heart or from Neurospora crassa was not inhibited by the antiseru

...ble protein component (Fc2) which was also required for the sensitivity of ATPase to dicyclohexylcarbodiimide. ...ability to bind ATPase (F1). Addition of Fc2 restored the ability to bind ATPase. It is therefore proposed that Fc2 is a component which links the mitochond

|description='''ATP synthase''' or F-ATPase (F₁F_O-ATPase; the use of Complex V is discouraged) catalyzes the [[endergonic]] phosphor

|title=Cyclophilin D stabilizes FOF1-ATPase dimers and cristae shape to modulate permeability transition pore. ...wide spectrum of insults, such as cardiac or brain ischemia. Although FOF1-ATPase dimers have been recently proposed as a core constituent of PTP, its comple

...ria during the hydrolysis of ATP to ADP and Pi by the oligomycin-sensitive ATPase show that almost 2H+ ions are translocated outwards through the M phase of ...translocation quotient (→H+/P) of 2, corresponding to that of the type II ATPase of the chemiosmotic hypothesis.

|title=Covi JA (2005) V-ATPase expression and function in the brine shrimp, ''Artemia franciscana'': Role ...quiescence in these embryos. Whole embryo respirometry demonstrates that V-ATPase activity and processes immediately dependent on that activity constitute ap

...n levels of CeRhr-1 and CeRhr-2, V-ATPase and Na⁺/K⁺-ATPase also increased significantly in response to 1 mmol l^-1 NH4Cl. |keywords=Vesicular transport, Na⁺/K⁺-ATPase, V-ATPase, Carbonic anhydrase

...and compensatory increase in the content of Complexes III and IV. The same ATPase deficiency without an increase in respiratory chain complexes was found in

...caused by different kinetic properties of soluble F,-ATPase and actomyosin ATPase. Therefore, phosphate seems to be in skeletal muscle in vivo only a modest

...uman muscle fibers by caffeine can be explained by a stimulation of myosin ATPase caused by Ca²⁺ release from sarcoplasmic reticulum.

the mitochondrial F1F0-ATPase begins to hydrolyze ATP to avoid the F1F0-ATPase, namely (i) reduction of the proton conductance of

...stae remodeling by Opa1 modulates mitochondrial bioenergetics by promoting ATPase dimerization and activity. ...al function upon CIII inhbition also prevented the generation of ROS in an ATPase dependent manner; accordingly, we observed lower cell death of Opa1<sup>tg<

|abstract=# The purification o f a soluble ATPase from beef heart mitochondria is described. The activity is depend ...ssed in relation to particulate mitochondrial ATPase and to myosin ATPase.

|abbr=ATPase (PM) |info='''A: Determination of ATPase activity in [[mitochondrial preparations]].

...medium with different 3BrPA concentrations promoted an increase in 40% in ATPase activity and protects the inhibition promoted by 3BrPA in calcium uptake ac |keywords=Sarco/endoplasmic reticulum calcium (Ca²⁺) ATPase (SERCA) type 1, 3-Bromopyruvate (3BrPA), White muscles, Sarco/endoplasmic r

...mitochondrial ATPase inhibitor were isolated from the rutamycin-sensitive ATPase complex of bovine heart mitochondria by heating and fractionation with etha

...hydrogenase can energize the vesicles directly without the intervention of ATPase or cytochromes.

...(nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.

...ta reported here suggest that the protonic inhibition of the mitochondrial ATPase plays a major role in regulating the rate of tissue ATP depletion during my

...013) Hypothetical ''Trypanosoma'' protein helps to anchor the F₁ATPase moiety to the mitochondrial membrane. Mitochondr Physiol Network 18.08. ...'ψ''_mt) is maintained by hydrolytic activity of the unique FoF1-ATPase, which contains several trypanosoma specific subunits of unknown function [

...), or ε (ATP5E gene) have been selected. The residual oligomycin-sensitive ATPase hydrolytic activity in these clones ranges between 2 and 78 % as compared t ...of these cells. On the other hand, the clones with more than 30 % residual ATPase activity showed no change either in the respiration or in their basal glyco

...the synthesis of ATPase subunit a, and causes the formation of incomplete ATPase complexes that are capable of ATP hydrolysis but not ATP synthesis. The mut

...ion]] The '''LEAK state with ATP''' is obtained in mt-preparations without ATPase activity after ADP is maximally phosphorylated to ATP ([[State 4]]; Chance ...LEAK respiration in the presence of ATP, ''L''_T, and absence of ATPase activity.]]

...i V (2010) Forward operation of adenine nucleotide translocase during F0F1-ATPase reversal: critical role of matrix substrate-level phosphorylation. FASEB J ...rated that, when the electron transport chain is compromised, the F(0)F(1)-ATPase reverses, and the membrane potential is maintained as long as matrix substr

...cribe a direct and flexible thin-layer chromatography method for detecting ATPase activity using radiolabeled ATP. Additionally, we describe a high-throughpu

...knowledge on the biochemistry of the interaction of IF(1) and the F(1)F(o)-ATPase, little is known about its physiological activity. Emerging research sugges

...oxidative phosphorylation, respiratory supercomplex, cell metabolism, F1F0-ATPase

...hosphorylation in skeletal muscle mostly via the stimulation of actomyosin ATPase.

...ther characterization of these clones revealed 2–78 % oligomycin-sensitive ATPase hydrolytic activity that parallels a decrease in the content of fully assem ...of these cells. On the other hand, the clones with more than 30 % residual ATPase activity displayed a change neither in the respiration nor in their basal g

...P, Dlaskova A (2018) Regulation of glucose-stimulated insulin secretion by ATPase Inhibitory Factor 1 (IF1). FEBS Lett 592:999-1009. |abstract=ATPase Inhibitory factor 1 (IF1) is an endogenous regulator of mitochondrial ATP s

...2021) Bioenergetic consequences of F_oF₁-ATP synthase/ATPase deficiency in two life cycle stages of ''Trypanosoma brucei''. J Biol Chem ...e inability to sustain ΔΨm. The diminishment of F_oF₁-ATPase complexes was further accompanied by a decreased ADP/ATP ratio and reduced

...rruda AP, Carvalho DP, de Meis L (2009) Cardiac sarcoplasmic reticulum Ca2-ATPase: Heat production and phospholamban alterations promoted by cold exposure an ...eart changes in oxygen consumption, SERCA2a-PLB ratio, and Ca²⁺-ATPase activity detected during short-term cold exposure were abolished after cold

...lly sensitive to energy limitation, but more sensitive than the Na(+)/K(+)-ATPase of the two fish species. A comparison of the indirect and direct methods fo ...ords=Ouabain, Cycloheximide, Energetics, Hepatocytes, HepG2 cells, Na+/K+- ATPase, Protein synthesis

...e combined action of nigericin and valinomycin in the presence of K+. Both ATPase activity and 32Pi—ATP exchange were inhibited by a specific antibody agai

...vation of heart mitochondrial oxidative phosphorylation: role of the F0/F1-ATPase. Am J Physiol Cell Physiol 278:C423-35.

...inhibitor of myosin-ATPase. Ca²⁺ -induced activation of myosin-ATPase also prevented the stimulation of respiration rates by creatine and AMP in

|keywords=glycolysis, oxygen consumption, temperature relationships, Na+/K+-ATPase, iodoacetic acid, ouabain, cycloheximide, ethionine, Carassius auratus, gol

...e CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal mu ...DP-stimulated respiratory kinetics. BLEB ('''blebbistatin'''), a myosin II ATPase inhibitor, blocked contraction under all conditions and yielded high ''K''<

...xpression of both K142A Walker A and E214Q Walker B mutants that an intact ATPase domain is essential for LACE-mediated degradation of nuclear-encoded comple

...pumping, thus supporting ATP production by the F₁F_O-ATPase even under CI inhibition.

...b> by combining the hydrolytic activity of the matrix-facing F₁-ATPase and the electrogenic exchange of ATP4- for ADP3- by the ADP/ATP carrier (AA

...n synthesis was suppressed in both cell types under hypoxia, whereas Na+K+-ATPase activity decreased in trout but not in goldfish hepatocytes, emphasising th |keywords=hypoxia, metabolic depression, protein synthesis, Na+/K+-ATPase, goldfish, Carassius auratus, rainbow trout, Oncorhynchus mykiss

...ered by the presence of adenosine. Adenosine inhibited normoxic Na(+)/K(+)-ATPase activity and K(+ )efflux by 20-35% in the cells of both species. An investi

...uction of ubiquinone supports ATP production by F₁F_O-ATPase in mitochondria with inhibited Complex I. https://doi.org/10.26124/mitofit: ...uction of ubiquinone supports ATP production by F₁F_O-ATPase in mitochondria with inhibited Complex I]<br/>

...ulum Ca²⁺ ATPase (SERCA). We found that calnexin stimulated the ATPase activity of SERCA by maintaining its redox state. This function enabled cal

...e to normoxic controls, including: (i) decreased citrate synthase and F1FO-ATPase activity but maintained protein content, (ii) markedly reduced aerobic capa |keywords=F1FO-ATPase, Anoxia/Reperfusion, Citrate synthase, Electron transport chain capacity, R

...bits: role of skeletal muscle mitochondria and sarcoplasmic reticulum Ca2+ ATPase isoform 1 heat production. Endocrinology 149:6262-71. ...e in the expression and activity of sarcoplasmic reticulum Ca²⁺ ATPase isoform 1 (SERCA1), one of the muscle enzymes involved in heat production.

...to 300–400 μM. However, when ADP production was initiated by intracellular ATPase reactions, the ADP concentration in the medium leveled off at about 40 μM |keywords=Muscle, Respiration, Mitochondrion, ADP, ATPase

...hospholipids and proteins in mussel gill mitochondria could allow the F1FO-ATPase activity to maintain the same activation energy as the mammalian enzyme. |keywords=F(1)F(O)–ATPase, Fatty acids, Laurdan, Mitochondrial membranes, Mussel

...embrane with the mitochondrial outer membrane of rats BAT. Ca²⁺-ATPase (SERCA 1) was identified by immunoelectron microscopy in both ER and mitoch |keywords=Brown adipose tissue, Ca²⁺-ATPase, mt-membrane potential, Respiration, Heat Production, ATP Synthesis

including: (i) decreased citrate synthase and F₁F_O-ATPase activity but maintained protein content, (ii) markedly reduced aerobic capa |keywords=F₁F_O-ATPase; Anoxia/reperfusion, Citrate synthase, Electron transport chain capacity, R

...of the two million invertebrate species, 2.7 protons are required by the F-ATPase to make each ATP molecule.

...evated in acute hypoxia. Our findings indicate that inhibition of the Na/K-ATPase by hypoxia contributes little to energy preservation in hypoxia. It remains

...ulphide bond, respectively. The recently determined structure of bovine F1-ATPase is locked in a conformation that probably represents the ADP-inhibited stat

...y to measure the ATP synthesized by reversal of the sarcoplasmic reticulum ATPase. ATP synthesized by adenylate kinase contaminating the sarcoplasmic reticul

|title=Garlid AO, Garlid KD (2012) Na,K-ATPase signaling to mitochondria. Mitochondr Physiol Network 17.12. |abstract=Binding of cardiac glycosides to the Na,K-ATPase has two effects: increased contractility (inotropy) and cardioprotection ag

...tabolism pathways upregulation, and increased Na⁺/K⁺ ATPase mRNA expression. Only ''in vivo'' treatment with NAC was able to reduce sei |keywords=2,4-dinitrophenol (DNP), N-acetylcysteine (NAC), Na+/K+ATPase, Audiogenic kindling, Glycolysis, Mitochondria, Reactive oxygen species, Wi

...(2013) Beating oxygen: chronic anoxia exposure reduces mitochondrial F1FO-ATPase activity in turtle (''Trachemys scripta'') heart. J Exp Biol 216:3283-93. ...ced aerobic capacity was the result of a severe downregulation of the F1FO-ATPase (Complex V), which we assessed as a decrease in enzyme activity. Furthermor

of ATPase subunit a and also affects the biogenesis of CcO. |keywords=Mitochondria, Cytochrome c oxidase, ATP synthase (ATPase), Tissue specificity, Protein assembly, Biogenesis, RNA interference (RNAi)

...iktorska M, Zylinska L (2014) Silencing of plasma membrane Ca²⁺-ATPase isoforms 2 and 3 impairs energy metabolism in differentiating PC12 cells. B ...up> in cytosol. In differentiation process plasma membrane Ca²⁺ ATPase (PMCA) is considered as one of the major players for Ca²⁺ homeos

...i V (2010) Forward operation of adenine nucleotide translocase during F0F1-ATPase reversal: critical role of matrix substrate-level phosphorylation. FASEB J ...d; B: ATP reverse, ANT forward; C, C1, C2: ATPase reverse, ANT reverse; D: ATPase forward, ANT reverse. Black solid triangles represent Erev_ATPase; white so

...tion or chemical permeabilization and avoiding the use of nonmitochondrial ATPase inhibitors. We conclude that our approach for characterizing effective ''P' |keywords=ATPase, Fluorescence, Magnesium green, Oxidative phosphorylation, Oxygraph

.../sub>-ATPsynthase in sub-mitochondrial particles and soluble F₁-ATPase. Activity assays and immunodetection of the inhibitor protein IF<sub>1</sub ...othyronamine (T1AM), F₀F₁-ATPsynthase, F₁-ATPase, IF₁, Aurovertin B, Resveratrol, Multiple inhibition kinetics, M

|abstract=The steroid Na⁺/K⁺ ATPase (NKA) blocker ouabain has been shown to exhibit pro-apoptotic effects in va ...m, ER, FBS, HeLa cells, Hist, InsP(3)R, MTT, Mitochondria, NKA, Na(+)/K(+)-ATPase, OD, Ouabain, Oub, Oub-BODIPY, RLU/s, St, TMRE, Z-LEHD-FMK, ZLF, [Ca(2+)](c

...stimulation on mRNA and protein expression of Na⁺,K⁺-ATPase, FXYD1, and FXYD5 in cultured human skeletal muscle cells. PLoS One 16:e024 |abstract=Denervation reduces the abundance of Na⁺,K⁺i-ATPase (NKA) in skeletal muscle, while reinnervation increases it. Primary human s

...cytes, protein expression levels of sarcoplasmic reticulum Ca²⁺-ATPase and phospholamban including two of its phosphorylated states (serine 16 and ...s. The protein expression levels of sarcoplasmic reticulum Ca²⁺-ATPase and phospholamban was not changed. Furthermore, we found a reduction in max

...dox state were correlated to ProDH activity and F₁F_O-ATPase directionality. ...embrane potential that was able to maintain the F₁F_O-ATPase operation in the forward mode. This was observed in CI-inhibited mouse live

...ochondrial respiration delta psi m is maintained by the mitochondrial F1F0-ATPase at the expense of ATP reserves. 6. In response to CN-_DOG, the initial rise

...s accordingly. High-quality isolated mitochondria contain zero or very low ATPase activity, such that resipration in the absence and presence of ATP is ident ...l_Acad_Sci_U_S_A |Gnaiger et al 2000]]). In permeabilized fibres with high ATPase activity, ATP might be added after stimulation of respiration by ADP in var

...eaching up to ∼69% (65 nmol mg^-1 of protein) and an increase in ATPase activity in intact mitochondria by ∼27% and ∼83% at 50 and 65 nmol mg<s |keywords=ATPase activity, Amidine derivatives, Liver mitochondria, Methoxyamidine, Mitochon

...oubled respiration at the necrotic phase in IZ. F₀F₁-ATPase activity decreased in the BZ and, to more extent, in IZ in both phases. Tra

...the uptake of protons. Inclusion of the mitochondrial oligomycin-sensitive ATPase during reconstitution yielded vesicles which catalyzed light-dependent phos

...signaling pathways linking ouabain binding to Na⁺-K⁺-ATPase with the opening of mitoK_ATP. In Langendorff-perfused rat hearts |keywords=Na⁺-K⁺-ATPase, Inotropy, Bradykinin, Signaling pathway, Reactive oxygen species

...ation in a temperature-sensitive manner which can be prevented with myosin-ATPase inhibitors [3,4]. ...e CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal mu

...ions generally and not only to protons, so that the electron transport and ATPase systems could be coupled through the sum of the '''electrical pressure diff ::::* (p 102) The proton-translocating respiratory chain and ATPase systems are assumed to be orientated in this membrane so that a displacemen

...properties strongly favored the conclusion that the coupling and ATPase activity were catalyzed by the same protein.

...hate (ATP) synthesis by a proton current that flows through the reversible ATPase system in the cristae membrane under a mainly electrical p.m.f., Baum [6] h

..., Pohjanpelto M, Lönnqvist T, Suomalainen A (2018) Defective mitochondrial ATPase due to rare mtDNA m.8969G>A mutation-causing lactic acidosis, intellectual

...ons can drive the synthesis of ATP by reversal of the proton-translocating ATPase independent of electron transport.

...+]i upon stimulation was due to increased sarco/endoplasmic reticulum Ca2+ ATPase (SERCA)-mediated reuptake rather than changes in Ca2+ influx capacity. Agon

|abstract=The chaperone-related AAA ATPase Cdc48 (p97/VCP in higher eukaryotes) segregates ubiquitylated proteins for

...tioxidants Trolox and N-acetyl-cysteine. Oligomycin, inhibitor of F(0)F(1)-ATPase, prevented hypoxia-induced HIF-1alpha protein accumulation and had no effec

...(SDS-, BN-, CN- and hrCN1-PAGE/WB) combined with western blot detection or ATPase in-gel activity assay. We also performed complex V immunocapture by IP and ...IM, Walker JE (2011) Binding of the inhibitor protein IF(1) to bovine F(1)-ATPase. J Mol Biol 406:443–53.

|title=Effects of inhibiting myosin-ATPase on mitochondrial respiratory capacity in permeabilized skeletal muscle. ...as to determine whether JO₂ in myofibers treated with myosin II ATPase inhibitor blebbistatin (bleb) during the entire sample preparation exhibit

...que structural features of TRAP1 likely regulate its temperature-sensitive ATPase activity and shed light on the alternative mechanisms driving the chaperone

...at permeabilized cardiomyocytes. To do this, we measured respiration rate, ATPase rate, and ADP concentration in the surrounding solution. The data were anal

...served with more severely aged mitochondria. The initial very low ATPase activity of the mitochondria was increased to appreciable magnitud

supply in the availability of ADP to F1-ATPase and of O2 and NO to cytochrome

...uctokinase (PFK), LDH) and at 20 min p.m. (PFK, F₀F₁-ATPase (F₀F₁)). Between 0 min and 10 min p.m. CS activ

...we predict that the F1Fo-ATP synthase is blocked during diapause by the F1-ATPase inhibitor protein IF1. This 9.6 kDa protein binds to the ATP synthase at th ...ley IM, Walker JE (2011) Binding of the inhibitor protein IF1 to bovine F1-ATPase. J Mol Biol 406: 443-453.

...r Factor 1 (IF1), a higher extent of IF1 bound to ATP synthase and a lower ATPase/synthase capacity were documented in JHH-6. Thus, here IF1 appears to down-

...search. Beyond adenylate interconversion via oxidative phosphorylation and ATPase activities, ADP and ATP act as steric regulators of enzymes, e.g. cytochrom

...fferential equations, and the basic components included are ANT, F(0)/F(1)-ATPase, and the phosphate carrier. The model reproduces quantitatively the relatio

...roteins were NADH-ubiquinone oxireductase subunit 4, subunit C of the F1F0-ATPase, and the adenine nucleotide transporters 1 and 2. Mitochondrial DNA/nuclear

were detected, while several ''Wolbachia'' F₁F₀-ATPase

...the mitochondrial bioenergetics. In all cases, molecular hybrids increase ATPase activity in coupled mitochondria, suggesting an uncoupled effect. All hybri

...ernating frogs decrease their ATP demands for Na⁺/K⁺-ATPase activity as part of a coordinated process of energy conservation wherein O<

...onsuming components of cellular energy budgets, the sodium pump ((Na⁺, K⁺)-ATPase), because of the removal of substrates for the tricarboxylic acid (TCA) cy

...C. similis'' gills showed the same levels of carbonic anhydrase and Na+/K+-ATPase activities and the same degree of enzyme induction during low-salinity adap

...roduced by ouabain inhibition of plasmalemmal Na⁺/K⁺-ATPase (NKA) is well established. However, the mechanism underlying this action is

...ntent decreased by 46%. A concomitant decrease of [ATP] as well as of Na,K-ATPase activity by over 50% could be prevented by incubating the cells in a Ca2+-f

|abstract=We have previously reported that transient knock-down of ATPase inhibitory factor 1 (IF1) by siRNA upregulates ATP levels and subsequently

...ription='''Sodium vanadate (Na₃VO₄)''' is used as an ATPase inhibitor.

|abstract=ATAD3 is a vital ATPase of the inner mitochondrial membrane of pluri-cellular eucaryotes with large

...xes I, III, and IV of the electron transport system, ATP synthesis at F1FO ATPase, substrate transporters including adenine nucleotide translocase and the ph

|abstract=Mitochondrial ATAD3A is an ATPase Associated with diverse cellular Activities (AAA) domain containing enzyme,

...f chemiosmotic coupling in which proton stoichiometries are 3 for the F1Fo-ATPase, 1 for the exchange of ATP for ADP and Pi, and 5, 4, and 4 for Sites 1, 2,

...ivity since it was not sensitive to the inhibitors of the sodium–potassium ATPase ouabain and of the mitochondrial adenine nucleotide translocator atractylos

...or of the mitochondrial F(1)F(0) adenosine triphosphate synthase (F(1)F(0)-ATPase), selectively increased superoxide and induced the apoptosis of alloreactiv

...oxygen consumption rate (OCR) was measured at baseline, after addition of ATPase inhibitor and subsequently after incubation in a range of propofol concentr ...ygen consumption rate (OCR) was measured at baseline and after addition of ATPase inhibitor, mitochondrial uncoupler and complex III inhibitor. Injection of

...rane potential maintenance, and ATP generation. Activity of the Na(+)/K(+)-ATPase is thereby diminished, ultimately decreasing the transport activity of the

...se agents effected a significant improvement in cardiac contractility. The ATPase activity of the "failed" mitochondria was normal. Experiments concerning th

...yruvate as substrate. The individual redox complexes and the H⁺-ATPase together occur at a density of approximately 7,500/µm² and occu

...the electron-transport chain (ETC). Together with the ATP-generating FoF1-ATPase (complex V or CV) the ETC complexes constitute the oxidative phosphorylatio

...atory capacity and augmented the abundance of Na⁺/K⁺-ATPase subunits (α1 and β1), ATP-sensitive K⁺ channel subunit (Kir6.2

|keywords=F(1)F(o)-ATPase, OPA1, Adenine nucleotide translocator, Aging, Cardiomyopathy, Complex I, C

...A. Whereas the activity of succinate dehydrogenase (SDH) and ATP synthase (ATPase) were diminished upon exposure to ONOO-, they were restored by pre-incubati

|abstract=Neurotensin is known to inhibit neuronal Na+ , K+ -ATPase, an effect that is rescued by nitric oxide (NO) synthase inhibition. Howeve

...ling. It lowered the rate of mitochondrial ATP synthesis, while increasing ATPase activity. These results indicate more than a single mitochondrial target of

..., enzymatic activities of citrate synthase, cytochrome c oxidase (COX) and ATPase were determined and gene expression was analysed by real-time PCR. The mito

...Here, we discover that sarcoplasmic/endoplasmic reticulum Ca²⁺ ATPase (SERCA2) phosphorylation at serine 663 is a clinical and pathophysiological

...cted in PS1 mutants, store size was similar. However, when Ca²⁺-ATPase activity was irreversibly inhibited with thapsigargin, the S170F and ΔE9 c

...respiratory chain complexes, oxygen consumption, ATP synthase assembly and ATPase hydrolytic activity.

|keywords=Adipose tissue, Calcium ATPase, Calcium-binding protein, Skeletal muscle, Uncoupling protein

...respiratory chain complexes, oxygen consumption, ATP synthase assembly and ATPase hydrolytic activity. On the other hand, we observed decreased fractional sh

...as an inhibitor of the respiratory chain component, sarcoplasmic reticulum ATPase, which leads to the release of Ca(2+) from the endoplasmic reticulum. Furth

...respiration media MiR05-Kit and Buffer Z in the presence of the myosin II ATPase inhibitor blebbistatin (25 µM) versus carrier; (3) ‘normoxia’ (200-100 ...e CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal mu

::::* [[ATPases|Unspecific ATPase activity]]

...associated with higher activity of sarcoplasmic reticulum Ca²⁺ -ATPase (SERCA) activity in the diaphragm of high-altitude mice.

...leak, we tested whether dantrolene, a sarco(endo)plasmic reticulum calcium ATPase leak inhibitor, would prevent mitochondrial and cardiac contractile dysfunc

...ology. Several biochemical methods, including blue native electrophoresis, ATPase enzyme activity assay, and mitochondrial oxygen consumption revealed a defe

...ydrolysis. This finding was consistent with a 50% depression of actomyosin ATPase activities. Superoxide production from SHR fibers was similar to that from

...ion vs. Bergstrom, such that respiration could be increased by a myosin II ATPase-inhibitor (Blebbistatin; BLEB). Eleven males and females each received a 2

...due to the ATP being diverted from the synthesis of biomass to the Na+, K+-ATPase pump. The dark heat flux was significantly (P=0.036) greater in the 3% trea

...t cellular energy consumers because of the reverse functioning of the F₁F₀-ATPase (complex V), which hydrolyzes ATP to pump protons out of the mitochondrial

...myocyte function by decreasing sarco-endoplasmic reticulum Ca²⁺-ATPase (SERCA) activity and delaying myofilament unbinding, disrupting cell relaxa

...s: 1. homogenate enriched with substrates was sequentially treated with an ATPase inhibitor (oligomycine), an uncoupler (FCCP) and KCN. This allowed us to me

...ently, our group showed that heme activates a Na⁺/K⁺ ATPase in these parasites through a signaling cascade involving hydrogen peroxide

...phosphate in TAC compared to control animals kinetically impair the myosin ATPase crossbridge cycle in decompensated hypertrophy/heart failure.

...ne 2016 Abstract MitoFit Science Camp 2016]]: Effects of inhibiting myosin-ATPase on mitochondrial respiratory capacity in permeabilized skeletal muscle.

...between the key cristae biogenesis protein Opa1 and Vwa8, a putative AAA+ ATPase with a dynein conformation. In order to study the role of Vwa8 protein in

...K respiration]]; T for ATP), or an overestimation of LEAK respiration if [[ATPase]] activity prevents final accumulation of ATP and maintains a continuous st

...en for aldolase inhibitors and show that it prevents FBP from binding to v-ATPase-associated aldolase and activates lysosomal AMPK, thereby mimicking a cellu

...iverted from the synthesis of biomass to the Na⁺, K⁺-ATPase pump. The dark heat flux was significantly (''P'' = 0.036) greater in the 3

...ripts encoding essential subunits for the mitochondrial cytochrome bc1 and ATPase complexes (i.a. accumulation of pre-RNA and strongly decreased levels of mR

...ton electrochemical potential gradient. (2) The F₁F_O ATPase is also vectorially oriented so that the energy of ATP hydrolysis drives ej

...y, novel inborn errors of metabolism were identified due to mutations in V-ATPase assembly factors TMEM199 and CCDC115. Patients are characterized by general

:::::::+ Evaluation of the ATPase contamination is included (2T).

...and after addition of pyruvate. Oligomycin (0.05 – 2.5 µM, inhibitor of F-ATPase) was added to obtain LEAK respiration. Electron transfer-capacity (''E'') w

...mpounds, such as oligomycin, which interfere with the function of the F1F0 ATPase. Brown fat present in infants and hibernating animals has thermogenin, a pr

...oligomycin, reaching to ~69% (65 nmol/mg of protein), and by the increased ATPase activity in intact mitochondria, by ~27% and ~64% to 50 and 65 nmol/mg of p

...atty acid as substrates. The adenine nucleotide translocase and Complex V (ATPase) are measured in the isolated mitochondria. Proteomic analysis is done usin

...patopathy. BCS1L is a nuclear gene that encodes a mitochondrial AAA-family ATPase. There is strong evidence that it acts as a chaperone that incorporates the

...f the adenine nucleotide translocase. We also directly measure Complex V (ATPase), and creatine kinase in frozen-thawed isolated mitochondria. Proteomic ana

...e CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal mu

...e content. Oxidative phosphorylation stoichiometry was unaffected, as were ATPase and adenine nucleotide translocator proteins and mRNAs. Mitochondrial acylc

# Zheng J, Ramirez VD (2000) Inhibition of mitochondrial proton F0F1-ATPase/ATP synthase by polyphenolic phytochemicals. Br J Pharmacol 130: 1115-23.

...examined. The inhibitory effect of D⁺ on the subunit c of FoF1-ATPase should be also considered. In order to understand the mechanisms involving

..., Merafina RS, Scartezzini P, Marra E, Vacca RA (2010) Impairment of F1F0-ATPase, adenine nucleotide translocator and adenylate kinase causes mitochondrial

...ndicates incomplete and inefficient metabolism of PM with no defect in the ATPase machinery in fructose-treated cells.

...px|thumb|LEAK respiration in the presence of ATP, ''L''(T), and absence of ATPase activity.]] [[LEAK state with ATP]]: classical [[State 4]] in isolated mito ...testing, if at State 4 (in a protocol defined by Chance and Williams 1955) ATPase activity is actually zero, such that respiration at State 4 is not partiall

...g protein^-1, ''n''=6, ''P''=0.008). In the presence of the F0F1-ATPase inhibitor, oligomycin, oxygen consumption significantly dropped to 0.09 ±

...e CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal mu

...activity is severely diminished in ATP6 [1] flies [1]. However, complex V ATPase activity is detectable and the membrane potential is not affected. These mu

...versus 74 nmol/min/mg). This difference in rate has been attributed to the ATPase acting as an ion-influx channel, because state 2 conditions favor the relea

...roperties of healthy and diabetic rat heart mitochondria: Mg²⁺- ATPase activity, content of conjugated dienes and membrane fluidity. Physiol Res S

...lateralis were obtained from the subjects for fibre type distribution with ATPase staining and measurement of mitochondrial performance with [[high-resolutio

...but on the posphorylation system including the F₁F_O-ATPase.

...e (10 mM), cytochrome (10 µM) and ADP (1.5 mM). Optimal concentration for ATPase inhibitor oligomycin was 1 µM and for uncoupler FCCP 2.75 µM without inhi

...e CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals dynamic range of mitochondrial respiratory control in skeletal musc

...er investigation as it could be linked to a reported increased activity of ATPase under hypoxia [4] or a defective OXPHOS with a possible enhanced ROS produc

|Robert Boushel - Inhibition of skeletal muscle ATPase activity alters mitochondrial substrate and adenylate control of OXPHOS, un ::::Inhibition of skeletal muscle ATPase activity alters mitochondrial substrate and adenylate control of OXPHOS, un

| ATPase (PM) | style="font-size:10px;" | ATPase (PM)

...e CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal mu

...e redox proton pumps; Fig. 1.9, 1.10; 4.3; 6.3; F₁F_O-ATPase is not a redox proton pump of the ETS, hence the term CV is not recommended

...myosin heavy chain and [[ouabain]] to inhibit Na⁺,K⁺-ATPase with rat heart homogenates.

...g</sub>/P» stoichiometry is the sum of the coupling stoichiometry in the F-ATPase reaction (­2.7 H⁺_pos from the positive intermembrane

...(Grenoble, Bordeaux) Influence of the mechanism of change in ATP synthase-ATPase. Stoichiometry on the control of oxidative phosphorylation ''in situ''. p 1

...iP2013| Hypothetical trypanosoma protein helps to anchor the F₁-ATPase moiety to the mitochondrial membrane]]||C6-04 Oral

...a, add oligomycin as a test for the possibility that ATPsynthase acts as a ATPase and thus maintains a mt-membrane potential in reversed mode of operation.

...ce of High-Fat Diet on Copper Metabolism in Mice: Involvement of the Cu(I)-ATPase ATP7B and Protein Kinase Cε (PKCε).

...vation of heart mitochondrial oxidative phosphorylation: role of the F0/F1-ATPase. Am J Physiol Cell Physiol 278:C423-35.

...oFit Science Camp 2016 | '''Kane DA''']] (CA) Effects of inhibiting myosin-ATPase on mitochondrial respiratory capacity in permeabilized muscle.

...IV proton pumping, supporting ATP production by F₁F_O-ATPase even when CI is inhibited"'''<br> ...uction of ubiquinone supports ATP production by F₁F_O-ATPase in mitochondria with inhibited Complex I. MitoFit Preprints 2022.1.v3. http

...vation of heart mitochondrial oxidative phosphorylation: role of the F0/F1-ATPase. Am J Physiol Cell Physiol 278:C423-35.]]

...vation of heart mitochondrial oxidative phosphorylation: role of the F0/F1-ATPase. Am J Physiol Cell Physiol 278:C423-35. - [[Territo 2000 Am J Physiol Cell

...ondrial oxidative phosphorylation: role of the F_O/F₁-ATPase. - [[Territo 2000 Am J Physiol Cell Physiol |»Bioblast link«]]

| ''' You can quantify the ATPase activity in your mitochondrial preparation:'''