Difference between revisions of "Scheibye-Knudsen 2009 Eur J Appl Physiol"
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{{Publication | {{Publication | ||
|title=Scheibye-Knudsen M, Quistorff B (2009) Regulation of mitochondrial respiration by inorganic phosphate: comparing permeabilized muscle fibers and isolated mitochondria prepared from type-1 and type-2 rat skeletal muscle. Eur | |title=Scheibye-Knudsen M, Quistorff B (2009) Regulation of mitochondrial respiration by inorganic phosphate: comparing permeabilized muscle fibers and isolated mitochondria prepared from type-1 and type-2 rat skeletal muscle. Eur J Appl Physiol 105:279-87. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/18989695 PMID: 18989695] | |||
|authors=Scheibye-Knudsen M, Quistorff B | |authors=Scheibye-Knudsen M, Quistorff B | ||
|year=2009 | |year=2009 | ||
|journal=Eur | |journal=Eur J Appl Physiol | ||
|abstract=ADP is generally accepted as a key regulator of oxygen consumption both in isolated mitochondria and in permeabilized fibers from skeletal muscle. The present study explored inorganic phosphate in a similar regulatory role. Saponin permeabilized fibers and isolated mitochondria from type-I and type-II muscle from male Wistar rats were prepared. Respiration was measured while the medium Pi concentration was gradually increased. The apparent Km values for Pi were 607 ± 17 µM and 405 ± 15 μM (P < 0.0001) for type-I and type-II fibers, respectively. For isolated mitochondria the values were significantly lower than type-1 permeabilized fibers, 338 ± 130 μM and 235 ± 30 μM (P < 0.05), but not different with respect to fiber type. The reason for this difference in Km values in the permeabilized muscle is unknown, but a similar pattern has been observed for K m of ADP. Our data indicate that phosphate may play a role in regulation of oxygen consumption ''in vitro'' and ''in vivo''. | |abstract=ADP is generally accepted as a key regulator of oxygen consumption both in isolated mitochondria and in permeabilized fibers from skeletal muscle. The present study explored inorganic phosphate in a similar regulatory role. Saponin permeabilized fibers and isolated mitochondria from type-I and type-II muscle from male Wistar rats were prepared. Respiration was measured while the medium Pi concentration was gradually increased. The apparent Km values for Pi were 607 ± 17 µM and 405 ± 15 μM (P < 0.0001) for type-I and type-II fibers, respectively. For isolated mitochondria the values were significantly lower than type-1 permeabilized fibers, 338 ± 130 μM and 235 ± 30 μM (P < 0.05), but not different with respect to fiber type. The reason for this difference in Km values in the permeabilized muscle is unknown, but a similar pattern has been observed for K m of ADP. Our data indicate that phosphate may play a role in regulation of oxygen consumption ''in vitro'' and ''in vivo''. | ||
|keywords=Mitochondrial respiration, Permeabilized muscle, Inorganic phosphate, Striated muscle, Oxygen consumption, Oxidative phosphorylation | |keywords=Mitochondrial respiration, Permeabilized muscle, Inorganic phosphate, Striated muscle, Oxygen consumption, Oxidative phosphorylation | ||
| | |mipnetlab=DK Copenhagen Quistorff B | ||
|discipline=Mitochondrial Physiology | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |organism=Rat | ||
|tissues=Skeletal | |tissues=Skeletal muscle | ||
|preparations=Isolated | |preparations=Permeabilized tissue, Isolated mitochondria | ||
| | |topics=Phosphate | ||
| | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
| | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Latest revision as of 11:31, 15 June 2015
| Scheibye-Knudsen M, Quistorff B (2009) Regulation of mitochondrial respiration by inorganic phosphate: comparing permeabilized muscle fibers and isolated mitochondria prepared from type-1 and type-2 rat skeletal muscle. Eur J Appl Physiol 105:279-87. |
Scheibye-Knudsen M, Quistorff B (2009) Eur J Appl Physiol
Abstract: ADP is generally accepted as a key regulator of oxygen consumption both in isolated mitochondria and in permeabilized fibers from skeletal muscle. The present study explored inorganic phosphate in a similar regulatory role. Saponin permeabilized fibers and isolated mitochondria from type-I and type-II muscle from male Wistar rats were prepared. Respiration was measured while the medium Pi concentration was gradually increased. The apparent Km values for Pi were 607 ± 17 µM and 405 ± 15 μM (P < 0.0001) for type-I and type-II fibers, respectively. For isolated mitochondria the values were significantly lower than type-1 permeabilized fibers, 338 ± 130 μM and 235 ± 30 μM (P < 0.05), but not different with respect to fiber type. The reason for this difference in Km values in the permeabilized muscle is unknown, but a similar pattern has been observed for K m of ADP. Our data indicate that phosphate may play a role in regulation of oxygen consumption in vitro and in vivo. • Keywords: Mitochondrial respiration, Permeabilized muscle, Inorganic phosphate, Striated muscle, Oxygen consumption, Oxidative phosphorylation
• O2k-Network Lab: DK Copenhagen Quistorff B
Labels:
Organism: Rat
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue, Isolated mitochondria
Regulation: Phosphate Coupling state: OXPHOS
HRR: Oxygraph-2k
