From Bioblast
Description
Reference: B: SUIT-018
SUIT number: D054_1GMS;2D;3Ama
O2k-Application: O2
- MitoPedia: SUIT
- SUIT protocol pattern: 1GMS;2D-
SUIT-018 O2 mt D054 is a short protocol to study the influence of a fluorescence dye on the O2 flux. During this protocol the [O2] can be modified by injecting nitrogen in the chamber.
Communicated by Komlodi T and Gnaiger E (last update 2019-09-24)
Representative traces
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1GMS | GMSL(n) | NS | CI&CII | 1GMS
|
2D | GMSP | NS | CI&CII | 1GMS;2D
|
2c | GMScP | NS | CI&CII | 1GMS;2D;2c
|
3Ama | ROX | 1GMS;2D;2c;3Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol can be used as a control for SUIT-018 AmR mt D031, SUIT-018 AmR mt D040 and SUIT-018 AmR mt D041. The oxygen concentration should be adjusted to the matching protocol with the Amplex UltraRed assay.
- + Reasonable duration of the experiment.
- - CIV activity and cytochrome c test can be performed.
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT B, Find
MitoPedia methods:
Respirometry