SUIT-018 O2 mt D054: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
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|description=[[File:1GMS;2D;2c;3Ama.png| | |description=[[File:1GMS;2D;2c;3Ama.png|290px]] | ||
|info='''B''': '''[[SUIT-018]]''' | |info='''B''': '''[[SUIT-018]]''' | ||
|application=O2 | |application=O2 | ||
|SUIT number= | |SUIT number=D054_mt;1GMS;2D;2c;3Ama | ||
}} | }} | ||
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: | {{Template:SUIT text D054}} | ||
SUIT | |||
__TOC__ | __TOC__ | ||
Β Communicated by | Β Communicated by [[Komlodi T]] and [[Gnaiger E]] (last update 2019-09-24) | ||
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== Representative traces == | |||
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[[File:SUIT-018 O2 mt D054.png|600px]] | |||
{{Template: SUIT-018 | {{Template: SUIT-018 O2_O2 Specific SUIT}} | ||
== Strengths and limitations == | == Strengths and limitations == | ||
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== Compare SUIT protocols == | == Compare SUIT protocols == | ||
== Chemicals and syringes == | |||
{{Template:Chemicals SUIT-018 O2 mt D054}} | |||
::: Suggested stock concentrations are shown in the specific DL-Protocol. | |||
Latest revision as of 12:08, 7 March 2024
Description
Reference: B: SUIT-018
SUIT number: D054_mt;1GMS;2D;2c;3Ama
O2k-Application: O2
SUIT-018 O2 mt D054 is a short protocol to study the influence of a fluorescence dye on the O2 flux. During this protocol the [O2] can be modified by injecting nitrogen in the chamber.
Communicated by Komlodi T and Gnaiger E (last update 2019-09-24)
Representative traces
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1GMS | GMSL(n) | NS | CI&CII | 1GMS
|
2D | GMSP | NS | CI&CII | 1GMS;2D
|
2c | GMScP | NS | CI&CII | 1GMS;2D;2c
|
3Ama | ROX | 1GMS;2D;2c;3Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol can be used as a control for SUIT-018 AmR mt D031, SUIT-018 AmR mt D040 and SUIT-018 AmR mt D041. The oxygen concentration should be adjusted to the matching protocol with the Amplex UltraRed assay.
- + Reasonable duration of the experiment.
- - CIV activity and cytochrome c test can be performed.
Compare SUIT protocols
Chemicals and syringes
Step | Chemical(s) and link(s) | Comments |
---|---|---|
1GMS | Glutamate (G), Malate (M) and Succinate (S) | |
2D | ADP (D) | |
2c | Cytochrome c (c) | |
3Ama | Antimycin A (Ama) |
- Suggested stock concentrations are shown in the specific DL-Protocol.
MitoPedia concepts:
SUIT protocol,
SUIT B,
Find
MitoPedia methods:
Respirometry