SUIT-018 O2 mt D054: Difference between revisions
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::: [[MitoPedia: SUIT | ::: [[MitoPedia: SUIT]] | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(GM) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(GM) | ||
::: '''[[SUIT protocol pattern]]:''' lineal 1GMS;2D;3Ama | ::: '''[[SUIT protocol pattern]]:''' lineal 1GMS;2D;3Ama |
Revision as of 15:37, 5 March 2019
Description
Abbreviation: NS(GM)
Reference: B: SUIT-018
SUIT number: D054_1GMS;2D;3Ama
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: NS(GM)
- SUIT protocol pattern: lineal 1GMS;2D;3Ama
SUIT-018 O2 mt D054 is a short protocol to study the influence of the fluorescence dye on the O2 flux. During this protocol the [O2] can be modified injecting nitrogen in the chamber.
Communicated by Komlodi T (last update 2019-03-05)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1GMS | GMSL | NS | CI&CII | 1GMS
|
2D | GMSP | NS | CI&CII | 1GMS;2D
|
3Ama | ROX | 1GMS;2D;3Ama
|
Strengths and limitations
- This protocol can be used as a control for SUIT-018 AmR mt D031, SUIT-018 AmR mt D040 and SUIT-018 AmR mt D041. The oxygen concentration should be adjusted to the matching protocol with the Amplex UltraRed assay.
- + Reasonable duration of the experiment.
- - CIV activity and cytochrome c test can be performed.
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT B, Find
MitoPedia methods:
Respirometry