SUIT-003 O2 ce D028: Difference between revisions
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|abbr=CCP_R 1Omy 2U 3Rot 4S 5Ama | |abbr=CCP_R 1Omy 2U 3Rot 4S 5Ama | ||
|description=[[File:Ce R 1Omy 2U 3Rot 4S 5Ama.jpg|right|200px]] | |description=[[File:Ce R 1Omy 2U 3Rot 4S 5Ama.jpg|right|200px]] | ||
'''[[Categories of SUIT protocols |SUIT-catg]]:''' | '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP_S | ||
|info= | |info= | ||
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:::* '''3Rot 4S 5Ama''' | :::* '''3Rot 4S 5Ama''' | ||
:::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[ | :::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[CCP_02]]; ''compare'' [[CCP_01]], Ce_R 1Omy 2U 3Rot). | ||
:::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated. | :::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated. | ||
:::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
Revision as of 19:57, 31 August 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D028
Description
SUIT-catg: CCP_S
Abbreviation: CCP_R 1Omy 2U 3Rot 4S 5Ama
MitoPedia concepts:
SUIT protocol
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP_R1
- DatLab-Excel template: in prep.: CCP_R1.xlsx
- 3Rot 4S 5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP_02; compare CCP_01, Ce_R 1Omy 2U 3Rot).
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
