Phosphate: Difference between revisions

Revision as of 07:59, 26 September 2018

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Phosphate

Description

Inorganic phosphate is a salt of phosphoric acid. In solution near physiological pH, the species HPO₄^2- and H₂PO₄^- dominate. See also: Phosphate carrier.

Reference: Scheibye-Knudsen 2009 Eur J Appl Physiol

MitoPedia topics: Substrate and metabolite

Comment

Most of the media used in HRFR contain inorganic phosphate (MiR05-Kit). It is not easy to isolate mitochondria in buffers without phosphate to study LEAK respiration limited by the absence of phosphate.

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 {{MitoPedia
 |abbr=
-|description=A '''phosphate''', an inorganic chemical, is a salt of phosphoric acid. In solutions (near physiological pH values) the species HPO<sub>4</sub><sup>2-</sup> and H<sub>2</sub>PO<sub>4</sub><sup>-</sup> will dominate.
+|description='''Inorganic phosphate''' is a salt of phosphoric acid. In solution near physiological pH, the species HPO<sub>4</sub><sup>2-</sup> and H<sub>2</sub>PO<sub>4</sub><sup>-</sup> dominate.
+''See also'': [[Phosphate carrier]].
 |info=[[Scheibye-Knudsen 2009 Eur J Appl Physiol]]
-|type=Respiration
 }}
 {{MitoPedia topics
 |mitopedia topic=Substrate and metabolite
-|type=Respiration
 }}
-'''Comment:'''
+::: '''Comment'''
-Most of the media used in [[HRR]] already contain phosphate because it is not that easy to isolate mitochondria in buffers without phosphate. Furthermore obtaining a Leak state without phosphate will not work that good.
+:::: Most of the media used in [[HRFR]] contain inorganic phosphate ([[MiR05-Kit]]). It is not easy to isolate mitochondria in buffers without phosphate to study [[LEAK]] respiration limited by the absence of phosphate.