Oses 2019 Epilepsy Res: Difference between revisions
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Revision as of 16:48, 18 March 2019
Oses JP, MΓΌller AP, Strogulski NR, Moreira JD, BΓΆhmer AE, Hansel G, Carteri RB, Busnello JV, Kopczynski A, Rodolphi MS, Souza DO, Portela LV (2019) Sustained elevation of cerebrospinal fluid glucose and lactate after a single seizure does not parallel with mitochondria energy production. Epilepsy Res 152:35-41. |
Oses JP, Mueller AP, Strogulski NR, Moreira JD, Boehmer AE, Hansel G, Carteri RB, Busnello JV, Kopczynski A, Rodolphi MS, Souza DO, Portela LV (2019) Epilepsy Res
Abstract: Generalized seizures trigger excessive neuronal firing that imposes large demands on the brain glucose/lactate availability and utilization, which synchronization requires an integral mitochondrial oxidative capability. We investigated whether a single convulsive crisis affects brain glucose/lactate availability and mitochondrial energy production. Adult male Wistar rats received a single injection of pentylentetrazol (PTZ, 60βmg/kg, i.p.) or saline. The cerebrospinal fluid (CSF) levels of glucose and lactate, mitochondrial respirometry, [14C]-2-deoxy-D-glucose uptake, glycogen content and cell viability in hippocampus were measured. CSF levels of glucose and lactate (meanβΒ±βSD) in control animals were 68.08βΒ±β11.62βmg/dL and 1.17βΒ±β0.32βmmol/L, respectively. Tonic-clonic seizures increased glucose levels at 10βmin (96.25βΒ±β13.19) peaking at 60βmin (113.03βΒ±β16.34) returning to control levels at 24βh (50.12βΒ±β12.81), while lactate increased at 10βmin (3.23βΒ±β1.57) but returned to control levels at 360βmin after seizures (1.58βΒ±β0.21). The hippocampal [14C]-2-deoxy-D-glucose uptake, glycogen content, and cell viability decreased up to 60βmin after the seizures onset. Also, an uncoupling between mitochondrial oxygen consumption and ATP synthesis via FoF1-ATP synthase was observed at 10βmin, 60βmin and 24βh after seizures. In summary, after a convulsive seizure glucose and lactate levels immediately rise within the brain, however, considering the acute impact of this metabolic crisis, mitochondria are not able to increase energy production thereby affecting cell viability.
Copyright Β© 2019 Elsevier B.V. All rights reserved. β’ Keywords: Cerebrospinal fluid, Glucose, Lactate, Mitochondria dysfunction, Seizure β’ Bioblast editor: Plangger M
Labels: MiParea: Respiration
Pathology: Other
Organism: Rat Tissue;cell: Nervous system Preparation: Homogenate
Coupling state: LEAK, ROUTINE, OXPHOS
Pathway: N, CIV, NS
HRR: Oxygraph-2k
Labels, 2019-03