MitoKit-CII/Malonate-nv: Difference between revisions
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::::# Add 395.78 ยตL DMSO in 7.7 ยตL NV161. | ::::# Add 395.78 ยตL DMSO in 7.7 ยตL NV161. | ||
::::# Divide into 40 ยตL portions. ย | ::::# Divide into 40 ยตL portions. ย | ||
::::# Store 6 | ::::# Store up to 6 months at -20 ยฐC or up to 1 week at room temperature. | ||
:::: '''Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):''' ย | :::: '''Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):''' ย | ||
::::# Dilute 40 ยตL 100 mM stock solution with 360 ยตL DMSO. | ::::# Dilute 40 ยตL 100 mM stock solution with 360 ยตL DMSO. | ||
::::# Divide into 100 ยตL portions.ย ย | ::::# Divide into 100 ยตL portions.ย ย | ||
::::# Store 6 | ::::# Store up to 6 months at -20 ยฐC or up to 1 week at room temperature. | ||
:::ยป '''O2k manual titrations''' | :::ยป '''O2k manual titrations''' |
Revision as of 08:47, 22 April 2020
Description
MitoKit-CII/Malonate-nv (diacetoxymethyl malonate) is a plasma membrane-permeable prodrug (permeable malonate; Mnanv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing malonate in the intracellular space. NeuroVive #: NV161
Abbreviation: Mnanv
Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
Application in HRR
- Malonate-nv: 10 mg, 7.7 ยตL, 5.24 M, MW=248.1 g.mol-1
- Preparation of 100 mM stock solution:
- Add 395.78 ยตL DMSO in 7.7 ยตL NV161.
- Divide into 40 ยตL portions.
- Store up to 6 months at -20 ยฐC or up to 1 week at room temperature.
- Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):
- Dilute 40 ยตL 100 mM stock solution with 360 ยตL DMSO.
- Divide into 100 ยตL portions.
- Store up to 6 months at -20 ยฐC or up to 1 week at room temperature.
- Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):
- ยป O2k manual titrations
- Event name: Mnanv
- Titration volume: 1 ยตL steps using a 10 ยตL syringe (2 mL O2k-Chamber).
- Final concentration: 5 ยตM steps.
- Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
- ยป O2k manual titrations