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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Hoppel Florian |
| Belgium | Namur | Arnould Thierry |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Detraux Damien |
| Belgium | Leuven | Bird Matthew |
| Belgium | Namur | Renard P |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Aarhus | Rattan Suresh IS |
| Denmark | Copenhagen | Desler C |
| Denmark | Malov | Hey-Mogensen M |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Copenhagen N | Mortensen OH |
| Egypt | Cairo | Ali Sameh S |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tartu | Grellier T |
| Estonia | Tallinn | Klepinina Lyudmila |
| Finland | Turku | Stier Antoine |
| Finland | Jyvaeskylae | Lehti Maarit |
| Finland | Helsinki | Pirinen E |
| France | Paris | Petit Patrice X |
| France | Bordeaux cedex | Mourier A |
| France | Bron | Rizwan Alam M |
| Germany | Leipzig | Ost Mario |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Freising | Keppner Gloria |
| Germany | Giessen | Eckert Gunter P |
| Germany | Ulm | Hartmann C |
| Germany | Munich | Jastroch M |
| Germany | Hamburg | Singer Dominique |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Biberach an der Riß | Nold V |
| Germany | Ulm | Kolassa Iris |
| Germany | Freising | Schoettl T |
| Germany | Duesseldorf | Eckers A |
| Germany | Ulm | Calzia Enrico |
| Germany | Gießen | Silaidos Carmina |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Ulm | Schaefer PM |
| Germany | Freising | Fromme Tobias |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Freising | Klingenspor Martin |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Debrecen | Verebne Tar K |
| Ireland | Dublin | Porter Richard K |
| Ireland | Sligo | Murphy J |
| Ireland | Dublin | Karavyraki Marilena |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Zrifin | Sova Marina |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Bologna | Paterlini S |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Italy | Bologna | Porcelli AM |
| Italy | Ancona | Battino Maurizio |
| Italy | Bologna | Gasparre G |
| Italy | Rome | Scatena Roberto |
| Italy | Milano | Bottoni P |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Bolzano | Pichler Irene |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Groningen | Rots MG |
| Netherlands | Wageningen | Keijer Jaap |
| Netherlands | Nijmegen | Koopman Werner JH |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Oslo | Lund Jenny |
| Norway | Oslo | Eide L |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Lodz | Siewiera Karolina |
| Poland | Warsaw | Wasilewski M |
| Poland | Krakow | Kaczara P |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Poznan | Michalak Slawomir |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Poznań | Ludziejewska A |
| Poland | Lodz | Watala Cezary |
| Poland | Poznan | Swiniuch Daria |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Portugal | Covilhã | Dias Tania R |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Bernardino RL |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Porto | Beleza Jorge |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Porto | Oliveira Pedro Fontes |
| Republic of Korea | Daejeon | Park SH |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Romania | Timisoara | Danila Maria-Daniela |
| Romania | Timisoara | Lelcu Theia |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Lighezan R |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Avram Vlad F |
| Russia | Moscow | Itkis YS |
| Russia | Moscow | Tsygankova P |
| Russia | Moscow | Krylova T |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Vukojevic M |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Nemec Michal |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Bratislava | Ukropcova B |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Slovenia | Ljubljana | Mars T |
| Spain | Valencia | Borras Consuelo |
| Spain | Madrid | Cano-Abad MF |
| Spain | Lleida | Nogueras L |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | A Coruña | Blanco FJ |
| Spain | Madrid | Monsalve Maria |
| Spain | Madrid | Lopez MG |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Sevilla | Muntane Jordi |
| Spain | Madrid | Cadenas S |
| Spain | Sevilla | Bustos Matilde |
| Spain | Salamanca | Almeida Angeles |
| Spain | Barcelona | Tura Ceide O |
| Spain | Barcelona | Arago Marc |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Barcelona | Smolders V |
| Spain | Granada | Quiles JL |
| Spain | Barcelona | Gil J |
| Spain | Valencia | Victor Victor Manuel |
| Spain | Barcelona | Juarez D |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | A Coruña | Mayan MD |
| Spain | Madrid | De los Rios C |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Armilla, Granada | Escames Germaine |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Abliva |
| Sweden | Lund | Westerlund E |
| Sweden | Lund | Chamkha Imen |
| Sweden | Lund | Karlsson M |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden | Lund | Elmer Eskil |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Geneva | Martinou JC |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Ankara | Engin Ayse Basak |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Izmir | Aydin HH |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Istanbul | Baykal AT |
| USA | St Louis | Welch R |
| USA | Philadelphia | Starr Jonathan |
| USA | Fayetteville | Rao RR |
| USA | Philadelphia | Janowska Joanna |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Baton Rouge | Irving Brian A |
| USA | Orlando | Coen Paul M |
| USA | Fayetteville | Iyer Shilpa |
| USA | Iowa City | Buettner Garry R |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Brighton | Moore Anthony L |
| United Kingdom | Birmingham | Joanisse S |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
Feedback WG4
- Dr. Ali will work within the realms of WG4 (blood and cultured cells) and potentially WG1 (protocol development) as well as WG5 (dissemination and training). He will participate in data generation, quality control, and repository efforts of protocols recommended by these working groups. Dr. Ali will contribute to training workshops and conferences that aim at defining standard procedures and terminology in the field of mitochondria physiology. - Sameh S Ali (2016).
- I am very interested in being part of the management committee either as a MC member for managment tasks or in working group 4 MITOEAGLE data repository for blood cells and cultured cells. I have experience in analyzing mitochondrial function in different human tissues as cultured cells and blood cells (also subsets of specific blood populations, see list of publications), muscle and isolated mitochondria. This together with my experience in organizing summer schools and conferences could be helpful as MC member taking care of management tasks. However, if you think my expertise is more helpful in working group 4, I am looking forward to support this working group. - Kathrin Renner-Sattler (2016).
