MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver: Difference between revisions
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|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CI, CII, CI&II | |substratestates=CI, CII, CI&II | ||
|instruments=Oxygraph-2k, O2k-Manual, Protocol | |instruments=Oxygraph-2k, O2k-Manual, O2k-Protocol | ||
|additional=Mt-Preparation, PBI-Shredder | |additional=Mt-Preparation, PBI-Shredder | ||
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Revision as of 17:34, 25 January 2016
Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Β»Bioblast pdfΒ« |
Β» Versions
OROBOROS (2015-06-16) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15(04):1-6.
A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS O2k, O2k-Catalogue
β’ Keywords: Tissue homogenate, Shredder
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart, Nervous system, Liver
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Manual, O2k-Protocol
Mt-Preparation, PBI-Shredder