Glucose: Difference between revisions

Latest revision as of 12:11, 15 June 2021

high-resolution terminology - matching measurements at high-resolution

Glucose

Description

Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.

Abbreviation: Glc

Reference: MiPNet15.09. Yeast: HRR Reference Assay

Application in HRR

Glc: Glucose (D-(+)-Glucose; C₆H₁₂0₆), Sigma-Aldrich: G8270, store at RT, CAS: 50-99-7, M = 180.16 g·mol^-1

2021-03: The preparation instructions were updated to take the volume of the solute into account (see: Volume of the solute).

Preparation of 2 M stock solution (1 mL, dissolved in H₂O):

weigh 360.32 mg of Glucose
dissolve in 775 µL H₂O in 1.5 mL Eppendorf

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
Final concentration: 20 mM

Preparation of 2.2 M stock solution (200 µL, dissolved in H₂O):

weigh 79.2 mg of Glucose
dissolve 155 µL H₂O in 0.5 mL Eppendorf

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
Final concentration: 5.5 mM (1 g/L)

Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
Final concentration: 11 mM (2 g/L)

MitoPedia topics: Substrate and metabolite

@@ Line 4: / Line 4: @@
 |info=[[MiPNet15.09. Yeast: HRR Reference Assay]]
 }}
-{{MitoPedia concepts}}
-{{MitoPedia methods}}
-{{MitoPedia O2k and high-resolution respirometry}}
-{{MitoPedia topics
-|mitopedia topic=Substrate and metabolite
-}}
-== Application in [[HRR]]  ==
-'''Glc:  Glucose''' (D-(+)-Glucose,  C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>);  Sigma G 8270, 100 g, store at R.T.; FW = 180.16
+__TOC__
+== Application in [[HRR]] ==
+{{Chemical_description
+|abbr=Glc
+|trivial name=Glucose
+|complete name=D-(+)-Glucose
+|chem formula=C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>
+|molar mass=180.16
+|vendor=Sigma-Aldrich
+|product number=G8270
+|store at=RT
+|sensitivity=
+|cas=50-99-7
+|h statements=
+|h info=
+}}<!--:::'''Glc:  Glucose''' (D-(+)-Glucose,  C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>);  Sigma G 8270, 100 g, store at RT; M = 180.16 g·mol<sup>-1</sup>-->
+:::: 2021-03: The preparation instructions were updated to take the volume of the solute into account (see: [[Volume_of_the_solute| Volume of the solute]]).
-'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O):
+::::'''Preparation of 2 M stock solution''' (1 mL, dissolved in H<sub>2</sub>O):
-::1) weigh 360.32 mg of Glucose
+::::# weigh 360.32 mg of Glucose
-::2) dissolve 1 ml H<sub>2</sub>O in 1,5 ml Eppendorf
+::::# dissolve in 775 µL H<sub>2</sub>O in 1.5 mL Eppendorf
-'''Oxygraph-2k Titration:'''
+:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::* Titration volume: 20 µl using a 50 µl syringe
+::::* Titration volume ('''2-mL O2k-chamber'''): 20 µL using a 50 µL Hamilton syringe.
-::* Final conc. in 2 ml O2k-chamber: 20 mM
+::::* Titration volume ('''0.5-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe.
+::::* Final concentration: 20 mM
-'''Preparation of 2.2 M stock solution''' (dissolved in H<sub>2</sub>O):
+::::'''Preparation of 2.2 M stock solution''' (200 µL, dissolved in H<sub>2</sub>O):
-::1) weigh 79.2 mg of Glucose
+::::# weigh 79.2 mg of Glucose
-::2) dissolve 0.2 ml H<sub>2</sub>O in 0,5 ml Eppendorf
+::::# dissolve 155 µL H<sub>2</sub>O in 0.5 mL Eppendorf
-'''Oxygraph-2k Titration:'''
+:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe.
+::::* Final concentration: 5.5 mM (1 g/L)
-::* Titration volume: 5 µl using a 25 µl syringe
+::::* Titration volume ('''2-mL O2k-chamber'''): 10 µL using a 25 µL Hamilton syringe.
-::* Final conc. in 2 ml O2k-chamber: 5.5 mM (1 g/L)
+::::* Final concentration: 11 mM (2 g/L)
+<!--
+::::* Titration volume: 5 µL using a 25 µL syringe
+::::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L)
-::* Titration volume: 10 µl using a 25 µl syringe
+::::* Titration volume: 10 µL using a 25 µL syringe
-::* Final conc. in 2 ml O2k-chamber: 11 mM (2 g/L)
+::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L)
+-->
+{{MitoPedia concepts}}
+{{MitoPedia methods}}
+{{MitoPedia O2k and high-resolution respirometry}}
+{{MitoPedia topics
+|mitopedia topic=Substrate and metabolite
+}}