Glucose: Difference between revisions

Revision as of 11:55, 10 November 2020

high-resolution terminology - matching measurements at high-resolution

Glucose

Description

Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.

Abbreviation: Glc

Reference: MiPNet15.09. Yeast: HRR Reference Assay

Application in HRR

Glc: Glucose (D-(+)-Glucose, C₆H₁₂0₆); Sigma G 8270, 100 g, store at RT; FW = 180.16

Preparation of 2 M stock solution (dissolved in H₂O):

weigh 360.32 mg of Glucose
dissolve 1 mL H₂O in 1.5 mL Eppendorf

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
Final concentration: 20 mM

Preparation of 2.2 M stock solution (dissolved in H₂O):

weigh 79.2 mg of Glucose
dissolve 0.2 mL H₂O in 0.5 mL Eppendorf

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
Final concentration: 5.5 mM (1 g/L)

Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
Final concentration: 11 mM (2 g/L)

MitoPedia topics: Substrate and metabolite

@@ Line 30: / Line 30: @@
 :::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
 ::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe.
-::::* Titration volume ('''0.5-mL O2k-chamber'''): 1.25 µL using a 10 µL Hamilton syringe.
 ::::* Final concentration: 5.5 mM (1 g/L)
 ::::* Titration volume ('''2-mL O2k-chamber'''): 10 µL using a 25 µL Hamilton syringe.
-::::* Titration volume ('''0.5-mL O2k-chamber'''): 2.5 µL using a 10 µL Hamilton syringe.
 ::::* Final concentration: 11 mM (2 g/L)