Glucose

high-resolution terminology - matching measurements at high-resolution

Glucose

Description

Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.

Abbreviation: Glc

Reference: MiPNet15.09. Yeast: HRR Reference Assay

MitoPedia topics: Substrate and metabolite 

Application in HRFR

Glc: Glucose (D-(+)-Glucose, C₆H₁₂0₆); Sigma G 8270, 100 g, store at R.T.; FW = 180.16

Preparation of 2 M stock solution (dissolved in H₂O):

1) weigh 360.32 mg of Glucose

2) dissolve 1 ml H₂O in 1,5 ml Eppendorf

Oxygraph-2k Titration:

• Titration volume: 20 µl using a 50 µl syringe
• Final conc. in 2 ml O2k-chamber: 20 mM

Preparation of 2.2 M stock solution (dissolved in H₂O):

1) weigh 79.2 mg of Glucose

2) dissolve 0.2 ml H₂O in 0,5 ml Eppendorf

Oxygraph-2k Titration:

• Titration volume: 5 µl using a 25 µl syringe
• Final conc. in 2 ml O2k-chamber: 5.5 mM (1 g/L)

• Titration volume: 10 µl using a 25 µl syringe
• Final conc. in 2 ml O2k-chamber: 11 mM (2 g/L)