Calcium Green: Difference between revisions
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Measurement: see [[O2k-Fluorescence LED2-Module]] | |||
Calcium green<sup>TM</sup> is a registered trademark and available from Invitrogen in several formulations. | |||
'''Measurement:''' see [[O2k-Fluorescence LED2-Module]] | |||
Calcium green and [[Magnesium green]] share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same fluorescence sensor and filter set can be used. Since the used concentration of Calcium green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium green. However, the values recommended for Magnesium green will provide a good starting point. | Calcium green and [[Magnesium green]] share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same fluorescence sensor and filter set can be used. Since the used concentration of Calcium green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium green. However, the values recommended for Magnesium green will provide a good starting point. |
Revision as of 15:34, 9 May 2012
Description
Calcium green belongs to the extrinsic fluorophores applied for measurement of Ca2+ concentration (KD = 190 nM.
Abbreviation: CaG
MitoPedia methods:
Fluorometry
Calcium greenTM is a registered trademark and available from Invitrogen in several formulations.
Measurement: see O2k-Fluorescence LED2-Module Calcium green and Magnesium green share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same fluorescence sensor and filter set can be used. Since the used concentration of Calcium green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium green. However, the values recommended for Magnesium green will provide a good starting point.