BAM15: Difference between revisions

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BAM15

2,4-dinitrophenol (C₆H₄N₂O₅; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.

Abbreviation: DNP

Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol

MitoPedia topics: Uncoupler

Preparation of 10 mM DNP stock solution (dissolved in H₂O):

Caution: Toxic!

1. Weigh 3.7 mg of DNP (FW = 184.11).

2. Dissolve in 1.2 ml H2O.

3. Neutralize with 1 N KOH.

4. Adjust final volume to 2 ml and divide into 0.2 ml portions.

6. Store at -20 °C.

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