From Bioblast
Description
Abbreviation: cePGlc,S
Reference: C SUIT-003 Second version of the Coupling control protocol and respirometric cell viability test (CCV protocol)
SUIT number: D018_ce1;ce1P;ce2Omy;ce3U;ce3Glc;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4Azd
O2k-Application: O2
- SUIT-category: cePGlc,S
- SUIT protocol pattern: complex cell coupling control protocol ce1;ce1P;ce2Omy;ce3U;ce3Glc;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4Azd
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce1P | ROUTINE | ce1;ce1P; ROUTINE, R
| ||
ce2Omy | L(Omy) | ce1;ce1P;ce2Omy
| ||
ce3U | E | ce1;ce1P;ce2Omy;ce3U
| ||
ce3Glc | E | ce1;ce1P;ce2Omy;ce3U;ce3Glc
| ||
ce4Rot | ROX | ce1;ce1P;ce2Omy;ce3U;ce3Glc;ce4Rot
| ||
ce5S | ce1;ce1P;ce2Omy;ce3U;ce3Glc;ce4Rot;ce5S
| |||
1Dig | SE | ce1;ce1P;ce2Omy;ce3U;ce3Glc;ce4Rot;ce5S;1Dig
| ||
1Ama | ROX | ce1;ce1P;ce2Omy;ce3U;ce3Glc;ce4Rot;ce5S;1Dig;2Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
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- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- The SUIT-003 O2 ce-pce D018 is a cell coupling control protocol. The total cell count (Nce) is the sum of viable cells (Nvce) and dead cells (Ndce).
- When MiR05 is used, the addition of pyruvate is recommended to provide an external fuel substrate.
- CIV activity can also be measured after the cell viability test module.
- + With the extended module, S-ET capacity can be assessed after the performance of the coupling control protocol, as well as outer mt-membrane integrity with the addition of cytochrome c.
- - The addition of oligomycin has to be carefully tested to avoid inhibitory effects in the evaluation of the ET-capacity.
- - Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler.
Compare SUIT protocols
- SUIT-003 O2 ce-pce D020 (A Advantageous alternative protocol)
- SUIT-003 O2 ce-pce D013
MitoPedia concepts: SUIT protocol, SUIT C, Find
MitoPedia methods:
Respirometry