Tsai 2016 Sci Rep
Tsai HH, Chang SC, Chou CH, Weng TP, Hsu CC, Wang JS (2016) Exercise training alleviates hypoxia-induced mitochondrial dysfunction in the lymphocytes of sedentary males. Sci Rep 6:35170. |
Tsai HH, Chang SC, Chou CH, Weng TP, Hsu CC, Wang JS (2016) Sci Rep
Abstract: This study elucidates how interval and continuous exercise regimens affect the mitochondrial functionality of lymphocytes under hypoxic stress. Sixty healthy sedentary males were randomly assigned to engage in either high-intensity interval training (HIIT, 3βmin intervals at 80% and 40% VO2max, nβ=β20) or moderate-intensity continuous training (MICT, sustained 60% VO2max, nβ=β20) for 30βmin/day, 5 days/week for 6 weeks or were assigned to a control group that did not receive exercise intervention (nβ=β20). Lymphocyte phenotypes/mitochondrial functionality under hypoxic exercise (HE, 100βW under 12% O2) were determined before and after the various interventions. Before the intervention, HE (i) increased the mobilization of senescent (CD57+/CD28-) lymphocytes into the blood, (ii) decreased the ATP-linked O2 consumption rate (OCR), the reserve capacity of OCR, and the citrate synthase activity in the mitochondria, and (iii) lowered the mitochondrial membrane potential (MP) and elevated the matrix oxidant burden (MOB) of lymphocytes. However, both HIIT and MICT significantly (i) decreased blood senescent lymphocyte counts, (ii) enhanced the mitochondrial OCR with increased citrate synthase and succinate dehydrogenase activities, (iii) increased mitochondrial MP and decreased MOB and (iv) increased the ratio of mitofusin to DRP-1 in lymphocytes after HE. Thus, we concluded that either HIIT or MICT effectively improves lymphocyte mitochondrial functionality by enhancing oxidative phosphorylation and suppressing oxidative damage under hypoxic conditions.
β’ O2k-Network Lab: TW Taoyuan Wang JS
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mt-Structure;fission;fusion, Exercise physiology;nutrition;life style
Stress:Oxidative stress;RONS Organism: Human Tissue;cell: Lymphocyte Preparation: Intact cells, Permeabilized cells
Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
Pathway: F, N, S, NS, Other combinations, ROX
HRR: Oxygraph-2k
2016-10