MitoKit-CII/Succinate-nv: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Snv | |abbr=Snv | ||
|description='''MitoKit-CII/Succinate-nv''' (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl | |description='''MitoKit-CII/Succinate-nv''' (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl groups is mediated by intracellular esterases, thus releasing [[succinate]] in the intracellular space. Abliva #: 01-118-s4 | ||
|info=[[MitoKit-CII | |info=[[MitoKit-CII]], [[Ehinger 2016 Nat Commun]] | ||
}} | }} | ||
__TOC__ | |||
== Application in HRR == | == Application in HRR == | ||
::: '''Succinate-nv:''' 25 mg, MW=262.2 g.mol<sup>-1</sup> | |||
:::: '''Preparation of 1 M stock solution:''' | :::: '''Preparation of 1 M stock solution:''' | ||
::::# Dissolve 25 mg Snv in 95.3 ยตL DMSO. | ::::# Dissolve 25 mg Snv in 95.3 ยตL DMSO. | ||
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::::# Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO. | ::::# Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO. | ||
::::# Divide into 50 ยตL portions.ย ย | ::::# Divide into 50 ยตL portions.ย ย | ||
::::# Store at -20 ยฐC. | ::::# Store up to 4 months at -20 ยฐC or up to 1 week at room temperature. | ||
:::ยป '''O2k manual titrations''' ย | :::ยป '''O2k manual titrations''' ย | ||
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::::* Final concentration: 50 ยตM steps. | ::::* Final concentration: 50 ยตM steps. | ||
::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type. | ::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type. | ||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
}} |
Latest revision as of 12:08, 27 July 2021
Description
MitoKit-CII/Succinate-nv (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl groups is mediated by intracellular esterases, thus releasing succinate in the intracellular space. Abliva #: 01-118-s4
Abbreviation: Snv
Reference: MitoKit-CII, Ehinger 2016 Nat Commun
Application in HRR
- Succinate-nv: 25 mg, MW=262.2 g.mol-1
- Preparation of 1 M stock solution:
- Dissolve 25 mg Snv in 95.3 ยตL DMSO.
- Preparation of 1 M stock solution:
- Succinate-nv: 25 mg, MW=262.2 g.mol-1
- Preparation of 100 mM stock solution of Snv (from the 1 M stock):
- Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO.
- Divide into 50 ยตL portions.
- Store up to 4 months at -20 ยฐC or up to 1 week at room temperature.
- Preparation of 100 mM stock solution of Snv (from the 1 M stock):
- ยป O2k manual titrations
- Event name: Snv
- Titration volume: 1 ยตL steps using a 10 ยตL syringe (2 mL O2k-Chamber).
- Final concentration: 50 ยตM steps.
- Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
- ยป O2k manual titrations
MitoPedia topics: Inhibitor