Octanoylcarnitine: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Oct | |abbr=Oct | ||
|description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]]. | |description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]], frequently applied as a substrate for [[fatty acid oxidation]] (FAO) of [[mitochondrial preparations]]. | ||
|info=[http://www.jbc.org/content/256/16/8371.full.pdfย ''pdf''] | |info=[http://www.jbc.org/content/256/16/8371.full.pdfย ''pdf''] | ||
|type=Substrate ETS | |type=Substrate ETS |
Revision as of 09:39, 3 September 2014
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) of mitochondrial preparations.
Abbreviation: Oct
Reference: pdf
MitoPedia topics: Substrate and metabolite
Carnitine O-octanoyltransferase is an enzyme that catalyzes the chemical reaction:
- octanoyl-CoA + L-carnitine โ CoA + L-octanoylcarnitine
Thus, the two substrates of this enzyme are octanoyl-CoA and L-carnitine, whereas its two products are CoA and L-octanoylcarnitine.
Application in HRR
Oct: Octanoylcarnitine (DL-Octanoyl-carnitine-HCL, C15H30NO4Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.
Alternative: (ยฑ)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 100 mM stock solution (dissolved in H2O):
- Weigh 32.4 mg of octanoylcarnitine and dissolve in 1 ml H2O.
- Divide into 0.2 ml portions.
- Store at -20 ยฐC.
Oxygraph-2k manual titrations into 2 ml O2k-chamber
- Titration volume: 4 ยตl using a 10 ยตl syringe.
- Final concentration: 0.2 mM in MiR05 or MiR06. Optimum Oct concentration has to be evaluated, particularly in media with BSA concentrations different from MiR06.