MiPNet18.05 Amplex-Mouse-heart: Difference between revisions
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|substratestates=CI, CII, CI+II | |substratestates=CI, CII, CI+II | ||
|instruments=Oxygraph-2k, Fluorometry, Protocol | |instruments=Oxygraph-2k, Fluorometry, Protocol | ||
|additional=O2k-Demo | |additional=O2k-Demo, mt-Preparation | ||
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Revision as of 23:10, 16 May 2014
Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. |
ยป Open Access-MiPNet18.05.pdf; Versions
OROBOROS (2013) Mitochondr Physiol Network
Abstract: O2k-Protocol for HRR and H2O2 production in mouse cardiac tissue homogenate.
The O2k-Fluorescence LED2-Module is an extension of the OROBOROS Oxygraph-2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplexยฎ Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
- >> Permeabilized muscle fibres
โข O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck MitoCom
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Heart
Preparation: Homogenate
Regulation: Cyt c Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo, mt-Preparation