MiPNet12.23 FiberRespiration: Difference between revisions
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Lemieux H, Votion DM, Gnaiger E | |title=Lemieux H, Votion DM, Gnaiger E (2007) Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23. | ||
|info=[http://www.oroboros.at/?protocols_muscle_horse MiPNet12.23] | |info=[http://www.oroboros.at/?protocols_muscle_horse MiPNet12.23] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year= | |year=2007 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph- | |abstract=Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph- | ||
Line 18: | Line 18: | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|topics=Coupling efficiency;uncoupling | |topics=Coupling efficiency;uncoupling, Cyt c | ||
|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CI, CII, CI+II | |substratestates=CI, CII, CI+II | ||
Line 27: | Line 27: | ||
}} | }} | ||
==Info== | ==Info== | ||
MiPNet04.07 is integrated in MiPNet12.23. | MiPNet04.07 is integrated in MiPNet12.23. | ||
* Version 1: 2007 | |||
* Version 6: 2011-12-11 |
Revision as of 08:29, 25 August 2013
Lemieux H, Votion DM, Gnaiger E (2007) Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23. |
ยป MiPNet12.23
OROBOROS (2007) Mitochondr Physiol Network
Abstract: Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph- 2k (O2k). The experiment demonstrates manual titrations applied to study respiratory capacity in permeabilized fibres. Application of the DatLab 4 software is shown for on-line data analysis MiPNet12.09 The following guideline describes the experimental protocol and includes a short discussion of results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 (IOC44; Schroecken, Austria).
โข O2k-Network Lab: AT_Innsbruck_OROBOROS, BE_Liege_Votion DM
Labels: MiParea: Respiration, Instruments;methods
Organism: Horse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Regulation: Coupling efficiency;uncoupling, Cyt c Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
Info
MiPNet04.07 is integrated in MiPNet12.23.
- Version 1: 2007
- Version 6: 2011-12-11