Octanoylcarnitine: Difference between revisions
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::::# Store at -20 ยฐC. | ::::# Store at -20 ยฐC. | ||
:::::* Note 2024-03-26: According to the information provided by the supplier (BioTrend) is the solubility in DMSO and H<sub>2</sub>O comparable, therefore we consider to use H<sub>2</sub>O as a solvent, which is | :::::* '''''Note 2024-03-26:''''' According to the information provided by the supplier (BioTrend) is the solubility of Octanoylcarnitine in DMSO and H<sub>2</sub>O comparable ( โฅ36.9 mg/mL in DMSO; โฅ18.6 mg/mL in EtOH; โฅ37.4 mg/mL in H<sub>2</sub>O), therefore we consider more rational approach to use H<sub>2</sub>O as a solvent in OROBOROS Lab because of better handling at room temperature. Disadvantage of DMSO: freezing point of +18.55 ยฐC (65.4 ยฐF). When using DMSO as solvent, make sure to bring the stock solution to room temperature before filling the syringe, which is important to avoid blocking the needle. | ||
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:::::: communicated by [[User:Sobotka Ondrej|Sobotka Ondrej]] ([[User talk:Sobotka Ondrej|talk]]) 17:46, 26 March 2024 (CET) | |||
:::ยป '''O2k manual titrations into 2 mL O2k-chamber''' ย | :::ยป '''O2k manual titrations into 2 mL O2k-chamber''' ย |
Revision as of 10:59, 4 April 2024
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.
Abbreviation: Oct
Reference: pdf
MitoPedia topics: Substrate and metabolite
Application in HRR
- Oct: Octanoylcarnitine ((ยฑ)-Octanoylcarnitine chloride; C15H30NO4Cl), BioTrend (brand: APExBIO Technology): B6371-50 mg, store at -20 ยฐC, CAS: 14919-35-8, M = 323.86 gยทmol-1
- Alternative: TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); M = 323.85 gยทmol-1.
- General tips: For obtaining a higher solubility, warm the tube at 37 โ and shake it in the ultrasonic bath for a while.
- Preparation of 100 mM stock solution (dissolved in H2O or DMSO, can differ from batch to batch - check Certificate of Analysis):
- Weigh 50 mg of octanoylcarnitine and dissolve in 1543 ยตL H2O or DMSO.
- Divide into 0.2 mL portions.
- Store at -20 ยฐC.
- Note 2024-03-26: According to the information provided by the supplier (BioTrend) is the solubility of Octanoylcarnitine in DMSO and H2O comparable ( โฅ36.9 mg/mL in DMSO; โฅ18.6 mg/mL in EtOH; โฅ37.4 mg/mL in H2O), therefore we consider more rational approach to use H2O as a solvent in OROBOROS Lab because of better handling at room temperature. Disadvantage of DMSO: freezing point of +18.55 ยฐC (65.4 ยฐF). When using DMSO as solvent, make sure to bring the stock solution to room temperature before filling the syringe, which is important to avoid blocking the needle.
- communicated by Sobotka Ondrej (talk) 17:46, 26 March 2024 (CET)
- ยป O2k manual titrations into 2 mL O2k-chamber
- Titration volume (2-mL O2k-chamber): 10 ยตL using a 25 ยตL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 2.5 ยตL using a 10 ยตL Hamilton syringe
- Final concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.
Labels:
MitoPedia:FAT4BRAIN