Fang 2021 Cell Rep: Difference between revisions
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|year=2021 | |year=2021 | ||
|journal=Cell Rep | |journal=Cell Rep | ||
|abstract=The assembly pathways of mitochondrial respirasome (supercomplex I+ | |abstract=The assembly pathways of mitochondrial respirasome (supercomplex I+III<sub>2</sub>+IV) are not fully understood. Here, we show that an early sub-complex I assembly, rather than holo-complex I, is sufficient to initiate mitochondrial respirasome assembly. We find that a distal part of the membrane arm of complex I (P<sub>D</sub>-a module) is a scaffold for the incorporation of complexes III and IV to form a respirasome subcomplex. Depletion of P<sub>D</sub>-a, rather than other complex I modules, decreases the steady-state levels of complexes III and IV. Both HEK293T cells lacking TIMMDC1 and patient-derived cells with disease-causing mutations in TIMMDC1 showed accumulation of this respirasome subcomplex. This suggests that TIMMDC1, previously known as a complex-I assembly factor, may function as a respirasome assembly factor. Collectively, we provide a detailed, cooperative assembly model in which most complex-I subunits are added to the respirasome subcomplex in the lateral stages of respirasome assembly. | ||
|keywords=Leigh syndrome, TIMMDC1, Cooperative assembly, Mitochondrial respirasome, Oxidative phosphorylation | |keywords=Leigh syndrome, TIMMDC1, Cooperative assembly, Mitochondrial respirasome, Oxidative phosphorylation | ||
|editor=[[Plangger M]] | |editor=[[Plangger M]] |
Revision as of 11:28, 20 April 2021
Fang H, Ye X, Xie J, Li Y, Li H, Bao X, Yang Y, Lin Z, Jia M, Han Q, Zhu J, Li X, Zhao Q, Yang Y, Lyu J (2021) A membrane arm of mitochondrial complex I sufficient to promote respirasome formation. Cell Rep 35:108963. |
Fang H, Ye X, Xie J, Li Y, Li H, Bao X, Yang Y, Lin Z, Jia M, Han Q, Zhu J, Li X, Zhao Q, Yang Y, Lyu J (2021) Cell Rep
Abstract: The assembly pathways of mitochondrial respirasome (supercomplex I+III2+IV) are not fully understood. Here, we show that an early sub-complex I assembly, rather than holo-complex I, is sufficient to initiate mitochondrial respirasome assembly. We find that a distal part of the membrane arm of complex I (PD-a module) is a scaffold for the incorporation of complexes III and IV to form a respirasome subcomplex. Depletion of PD-a, rather than other complex I modules, decreases the steady-state levels of complexes III and IV. Both HEK293T cells lacking TIMMDC1 and patient-derived cells with disease-causing mutations in TIMMDC1 showed accumulation of this respirasome subcomplex. This suggests that TIMMDC1, previously known as a complex-I assembly factor, may function as a respirasome assembly factor. Collectively, we provide a detailed, cooperative assembly model in which most complex-I subunits are added to the respirasome subcomplex in the lateral stages of respirasome assembly. โข Keywords: Leigh syndrome, TIMMDC1, Cooperative assembly, Mitochondrial respirasome, Oxidative phosphorylation โข Bioblast editor: Plangger M
Labels: MiParea: Respiration
HRR: Oxygraph-2k
2021-04