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| {{MitoPedia
| | #REDIRECT [[MitoKit-CII]] |
| |abbr=MitoKit-CII
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| |description='''Cell permeable prodrugs''', composed of [[NV161]] and [[NV118]], of the Complex II substrate succinate increase ATP-linked mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres.
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| |info=[[MitoKit-CII]], [[Ehinger 2016 Nat Commun]]
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| }}
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| {{MitoPedia topics
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| |mitopedia topic=Medium
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| }}
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| * For preparation, see [[MitoKit-CII]].
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| == Application in [[HRR]] ==
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| === Preparation ===
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| :::: Both compounds are dissolved in DMSO for 1M concentration stock solution and stored in aliquots at -20.
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| :::: We recommend the following dilutions for reaching the desired concentration in the experiments:
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| ::::* NV118: 100 mM stock solution
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| ::::* NV161: 10 mM stock solution
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| == Expiry date of MitoKit-CII compounds (according to NeuroVive) ==
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| Before preparation:
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| ::::* 3 years in -80° C
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| ::::* 1 year in -20° C
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| Prepared:
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| ::::* In DMSO solution
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| ::::::* at room temperature: 24 hours.
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| ::::::* at -20° C: for weeks
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