MitoPediaAlert2020 Archive: Difference between revisions
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== Jun 2020 == | == Jun 2020 == | ||
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| width="110" align="center" | [[ | | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | ||
| ''' | | '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br> | ||
- » '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''« | |||
-»'''[ | |||
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* '''Jun- | |||
* '''Jun-18 Th''' | |||
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| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | ||
| ''' | | '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?''' | ||
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br> | |||
- »'''[[SUIT-003 O2 ce D037]]'''« and »'''[[SUIT-003 O2 ce D038]]'''« | |||
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* '''Jun-08 Mo''' | |||
* '''Jun- | |||
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| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | ||
| ''' | | '''Celebrating World Oceans Day'''<br> | ||
[[ | |||
- »'''[[ | Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br> | ||
- »'''[[PhotoBiology]]'''« | |||
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* '''Jun- | * '''Jun-04 Th''' | ||
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| width="110" align="center" | [[ | | width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | ||
| ''' | | '''Does your sample display a preference for [[GM|glutamate & malate]] over [[PM|pyruvate & malate]] to support NADH-linked respiration?''' <br> | ||
- » '''[ | DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates. <br> | ||
-»'''[[SUIT-008]]'''« and »'''[[SUIT-014]]'''« | |||
|} | |} | ||
Revision as of 08:17, 20 July 2020
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
Jul 2020
- Jul-23 Th
Do you know how to assess the integrity of the mitochondrial outer membrane in mitochondrial preparations? It is possible to do so using exogenously added cytochrome c:
- »Cytochrome c control factor«
- Jul-16 Th
Do you know how to select the proper Fluorescence-Sensor and filter set?
Using different Fluorescence-Sensors and Filters, the O2k-Fluo Smart-Module allows for measurement of Hydrogen peroxide, ATP, Mitochondrial membrane potential, Calcium, and numerous other applications open for O2k-user innovation.
- » Selecting the Smart Fluo-Sensor« and » Setup of the O2k-Fluo LED2-Module«
- Jul-09 Th
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?
- Jul-02 Th
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:
- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity. Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«