MiPNet06.03 POS-calibration-SOP: Difference between revisions
No edit summary |
No edit summary |
||
Line 19: | Line 19: | ||
|additional=O2k-SOP | |additional=O2k-SOP | ||
}} | }} | ||
'''This article is cited in''' | '''This article is cited in''' | ||
Line 34: | Line 32: | ||
== References == | == References == | ||
* [[Gnaiger_2008_POS]] | * [[Gnaiger_2008_POS]] | ||
* | * [[MiPNet19.18D_O2k-Calibration]] | ||
Β | |||
[[Category:Technical service]] | [[Category:Technical service]] |
Revision as of 22:30, 6 February 2016
MiPNet06.03 POS-calibration-SOP
Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Β»Bioblast pdfΒ« |
OROBOROS (2015-05-10) Mitochondr Physiol Network
Abstract: Gnaiger E (2015) Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Mitochondr Physiol Network 06.03(14):1-8.
High-resolution respirometry critically depends on accurate calibration of the polarographic oxygen sensors which respond to partial oxygen pressure. Expressing the oxygen signal in terms of partial pressure has several advantages, but respiration is expressed in molar units related to biochemical stoichiometries. Conversion of oxygen partial pressure to oxygen concentration requires accurate information on oxygen solubilities in experimental incubation media. Absolute errors up to 10% are commonly found in the established literature. The oxygen solubility of mitochondrial respiration medium MiR06 relative to pure water (oxygen solubility factor, FM) is 0.92, accurately determined (for MiR05, i.e. MiR06 without catalase; MiPNet14.13) at 37 Β°C and 30 Β°C. At air saturation at standard barometric pressure (100 kPa) and 37 Β°C, the partial oxygen pressure is 19.63 kPa, and the oxygen concentration is 190.7 Β΅M in MiR06. A clean and ready-to-use O2k-Chamber is a requirement for saving time and quickly calibrating the polarographic oxygen sensors.
- Β» O2k-Manual: Β»MiPNet19.18D_O2k-CalibrationΒ«
- Β» Product: OroboPOS, O2k, O2k-Catalogue
- Β» Template: O2k Calibration List
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, O2k-Protocol
O2k-SOP
This article is cited in
- Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. Β»PubMed 18370948Β«
Further details
- Next step of instrumental quality control: Β»MiPNet14.06 InstrumentalO2BackgroundΒ«
- Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. Β»Bioblast AccessΒ«