Beryllium sulfate: Difference between revisions
From Bioblast
(Created page with "{{MitoPedia |abbr=BeS |description='''Beryllium sulfate''' is used in combination with sodium fluoride to form beryllium trifluoride (BeF<sup>3โ</sup>), to inhibit the [...") ย |
No edit summary ย |
||
(One intermediate revision by the same user not shown) | |||
Line 4: | Line 4: | ||
}} | }} | ||
__TOC__ | __TOC__ | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
{{Chemical_description | {{Chemical_description | ||
|abbr=BeS | |abbr=BeS | ||
Line 12: | Line 10: | ||
|complete name=Beryllium sulfate | |complete name=Beryllium sulfate | ||
|chem formula=BeSO<sub>4</sub> | |chem formula=BeSO<sub>4</sub> | ||
|molar mass= ย | |molar mass= 177.14 | ||
|vendor= ย | |vendor= ย | ||
|product number= ย | |product number= ย | ||
|store at= | |store at=4 ยฐC | ||
|sensitivity= | |sensitivity= | ||
|cas= ย | |cas= ย | ||
Line 21: | Line 19: | ||
|h info= | |h info= | ||
}} | }} | ||
:::: '''Preparation of 200 mM stock solution''' (dissolved in H<sub>2</sub>O) | :::: '''Preparation of 200 mM stock solution''' (dissolved in H<sub>2</sub>O) | ||
ย | ::::# Weigh 354.3 mg. | ||
ย | ::::# Dissolve in 10 mL distilled H2O under a fume hood. | ||
::::# Divide in aliquots. | |||
::::# Store at +4 ยฐC; | |||
:::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 2 ยตL using a 10 ยตL syringe (2 mL O2k-chamber). | |||
::::* Final concentration: 200 ยตM. | |||
== References == | == References == | ||
::::* [ | ::::* [[Chinopoulos 2014 Methods Enzymol]] | ||
{{MitoPedia concepts}} | {{MitoPedia concepts}} |
Latest revision as of 12:57, 26 April 2024
Description
[[Description::Beryllium sulfate is used in combination with sodium fluoride to form beryllium trifluoride (BeF3โ), to inhibit the ATP synthase if it is exposed by disruption of the mitochondrial membranes.]]
Abbreviation: BeS
Application in HRR
- BeS: (Beryllium sulfate; BeSO4), store at 4 ยฐC, M = 177.14 gยทmol-1
- Preparation of 200 mM stock solution (dissolved in H2O)
- Weigh 354.3 mg.
- Dissolve in 10 mL distilled H2O under a fume hood.
- Divide in aliquots.
- Store at +4 ยฐC;
- Preparation of 200 mM stock solution (dissolved in H2O)
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 2 ยตL using a 10 ยตL syringe (2 mL O2k-chamber).
- Final concentration: 200 ยตM.
References
MitoPedia topics: Inhibitor