Gabriel 2017 Thesis
| Gabriel C (2017) Avaliação da função mitocondrial em idosos institucionalizados com e sem Doença de Alzheimer. Dissertation p55. |
Gabriel C (2017) Dissertation
Abstract: The longevity of the developed countries has increased and with it the frequency of degenerative diseases. Alzheimer's disease (AD) has a high incidence (60 to 70%) and is characterized by a progressive deterioration of the brain associated with the accumulation of amyloid plaques and neurofibrillary tangles (Oddo, 2012). AD causes progressive and irreversible deterioration of various cognitive functions, including memory, attention, concentration, language, thinking and behaviour (Burns & Iliffe, 2009). The loss of autonomy of the elderly is aggravated in the cases of AD, which often culminates in institutionalization. While the major risk factor for AD is age, the process is distinguished from normal aging. Although there are common changes and manifestations, probably supported by similar molecular and cellular changes, in AD brain aging process is aggravated. Literature have attributed an important role to oxidative stress and mitochondrial alterations either in the aging process or the AD. Thus, this study aims to understand the changes associated with normal aging process in mitochondria function and to identify some of the key differences in individuals with AD.
The sample was composed by 14 normal old subjects (80.57.7 years) and 11 AD (83.27.7 years) participated in this study. All the subjects were institutionalized and the diagnose of AD was in charge of a clinical Neurologist. After 9 hours of fasting condition, 10ml of blood were collected by venous puncture into the vacuum K3EDTA tubes and then centrifuged to separate serum, and to obtain lymphocytes. Mitochondria function [respiration in state 3 and state 4, and respiratory control ratio (RCR=state 3/state 4)] were measured in lymphocytes cells in Oroboros, according to the equipment procedures. Malondialdehyde Lipid peroxidation (Thiobarbituric reaction products, TBARs) was assayed in plasma samples by spectrophotometic method. Protein content was determined by Biureto method. To compare average values of both groups, a t-test for independent samples was used. Significance level was set a 0.05. The obtained results failed to found significant differences between groups in mitochondria respiration in states 3 (1.77±0.67 and 1.80±1.07 nmolO2/min/10 6 for normal and AD, respectively) and 4 (0.56±0.33 and 1.55±0.77 nmolO2/min/106 for normal and AD subjects, respectively). Significant changes between groups were found in RCR (3.76±1.54 and 2.33±1.36 for normal and AD, respectively) (t=2.553; p=0.017) and in plasma TBARS (15.796±4.098 and 11.169±4.459 nmol/mg of protein for normal and AD, respectively) (t=2.230; p=0.041). These results revealed that comparatively with AD, normal aged subjects evidence “healthy” mitochondria once they show high respiratory control (RCR). Nevertheless, the amount of oxidative stress damage (MDA) was higher in normal old subjects, possibly due to the influence of other unconsidered variables (medication, diet and daily physical activity). • Keywords: Aging, Mitochondria function, Alzheimer’s Disease, Oxidative stress • Bioblast editor: Kandolf G
Labels: MiParea: Respiration, Patients
Pathology: Alzheimer's
Stress:Oxidative stress;RONS
Organism: Human
Tissue;cell: Lymphocyte
Coupling state: LEAK, OXPHOS
HRR: Oxygraph-2k
2017-08
