Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Brewer 2004 Mitochondrion

From Bioblast
Publications in the MiPMap
Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with anti-oxidants. Mitochondrion 4:49-57.

» PMID: 16120374

Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Mitochondrion

Abstract: We tested the effect of an anti-oxidant mixture on respiration in isolated rat brain mitochondria. Mitochondria were isolated in mannitol/sucrose/EGTA/BSA±SCAVEGR™ anti-oxidants (SOD, catalase, vitamin E, vitamin E acetate, and glutathione reduced). TBARS were reduced by greater than 40% with SCAVEGR. Respiration driven by ADP showed a two-fold higher Vmax and a 15% higher respiratory control ratio when mitochondria were prepared with SCAVEGR. SCAVEGR also stabilized the octameric state of mitochondrial creatine kinase and thus improved creatine-stimulated respiration. These results suggest that significant improvements in brain mitochondrial function are obtained by isolation in the presence of an anti-oxidants mixture. Keywords: Anti-oxidant, Brain mitochondria, Creatine kinase, Respiration, ROS

O2k-Network Lab: CH Zurich Wallimann T, US IL Springfield Brewer GJ, FR Grenoble Schlattner U

Cited by

Gnaiger 2020 BEC MitoPathways
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002



Labels:

Stress:Oxidative stress;RONS  Organism: Rat  Tissue;cell: Nervous system  Preparation: Isolated mitochondria 



HRR: Oxygraph-2k 

BEC 2020.2