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Beauvoit 1999 Biochim Biophys Acta

From Bioblast
Publications in the MiPMap
Beauvoit B, Bunoust O, Guรฉrin B, Rigoulet M (1999) ATP-regulation of cytochrome oxidase in yeast mitochondria. Role of subunit VIa. Eur J Biochem 263:118-27.


Beauvoit B, Bunoust O, Guerin B, Rigoulet M (1999) Eur J Biochem

Abstract: The role of the nuclear-encoded subunit VIa in the regulation of cytochrome oxidase by ATP was investigated in isolated yeast mitochondria. As the subunit VIa-null strain possesses a fully active and assembled cytochrome oxidase, multiple ATP-regulating sites were characterized with respect to their location and their kinetic effect:

(a) intra-mitochondrial ATP inhibited the complex IV activity of the null strain, whereas the prevailing effect of ATP on the wild-type strain, at low ionic strength, was activation on the cytosolic side of complex IV, mediated by subunit VIa. However, at physiological ionic strength (i.e. <200 mm), activation by ATP was absent but inhibition was not impaired; (b) in ethanol-respiring mitochondria, when the electron flux was modulated using a protonophoric uncoupler, the redox state of aa3 cytochromes varied with respect to activation (wild-type) or inhibition (null-mutant) of the cytochrome oxidase by ATP; (c) consequently, the control coefficient of cytochrome oxidase on respiratory flux, decreased (wild-type) or increased (null-mutant) in the presence of ATP; (d) considering electron transport from cytochrome c to oxygen, the response of cytochrome oxidase to its thermodynamic driving force was increased by ATP for the wild-type but not for the mutant subunit. Taken together, these findings indicate that at physiological concentration, ATP regulates yeast cytochrome oxidase via subunit-mediated interactions on both sides of the inner membrane, thus subtly tuning the thermodynamic and kinetic control of respiration. This study opens up new prospects for understanding the feedback regulation of the respiratory chain by ATP. โ€ข Keywords: ATP regulation, Cytochrome c oxidase, Mitochondria, Saccharomyces cerevisiae, ubunit VIa.

โ€ข O2k-Network Lab: FR Pessac Diolez P, FR Bordeaux Devin A


Labels: MiParea: Respiration 


Organism: Saccharomyces cerevisiae, Fungi 

Preparation: Oxidase;biochemical oxidation  Enzyme: Complex IV;cytochrome c oxidase  Regulation: ADP, ATP 


HRR: Oxygraph-2k