https://wiki.oroboros.at/api.php?action=feedcontributions&user=Gnaiger+Erich&feedformat=atomBioblast - User contributions [en]2024-03-29T09:49:21ZUser contributionsMediaWiki 1.36.1https://wiki.oroboros.at/index.php?title=Template:Keywords:_O2k&diff=246713Template:Keywords: O2k2024-03-29T08:20:32Z<p>Gnaiger Erich: </p>
<hr />
<div><br /><br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242"> '''»Bioblast links: O2k and HRR''' - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
:::: '''O2k'''<br />
::::::» [[Oroboros O2k]]<br />
::::::» [[High-resolution respirometry]]<br />
::::::» [[NextGen-O2k]] - [[:Category:NextGen-O2k |Category:NextGen-O2k]] - [[NextGen-O2k Technical developments]]<br />
::::::» [[O2k-J]]<br />
::::::» [[MitoPedia: O2k hardware]]<br />
<br />
:::: '''Previous'''<br />
::::::» [[O2k-FluoRespirometer]]<br />
<br />
:::: '''Oroboros Marketplace'''<br />
::::::» [https://www.oroboros.at/index.php/o2k-products/ O2k]<br />
<br />
</div><br />
</div><br />
<br /><br />
<br />
[[Category:Keywords]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=O2k-J&diff=246712O2k-J2024-03-29T08:13:17Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Oroboros highlights page name}}<br />
<br />
__TOC__<br />
<br /><br />
<br />
== O2k-J ==<br />
:::: <big>Oroboros - as a driving force in mitochondrial physiology - extends the analytical and diagnostic power of high-resolution respirometry by integration of the [[O2k-Fluo Smart-Module]] and the [[PB-Module]] for PhotoBiology. We aim at establishing the Oroboros quality control management to address the acute ''reproducibility crisis'' of scientific investigation, to resolve the ''inflation crisis'' and ultimately the ''value-impact crisis'' of present academic publication. </big><br />
<br />
<br />
::::» [[MiPNet28.08 Oroboros O2k Series J manual |Oroboros O2k-J Manual]]<br />
<br />
{{Template:Keywords: O2k}}<br />
<br />
<br />
== Gentle Science ==<br />
<br />
:::: In addition to O2k-Workshops and on-site training, Oroboros will disseminate the not-for-profit initiatives on Mitochondrial Physiology and Bioenergetics Science Camps, specialized Mitochondrial Physiology Conferences, and Preprint publications:<br />
::::::» Oroboros is a key player in support of the [[MitoEAGLE |'''COST Action MitoEAGLE''']] <br />
::::::» '''[[MitoGlobal]]''' - the world-wide platform for societies and organizations supporting mitochondrial research and medicine<br />
::::::» MitoEAGLE with cooperation by Oroboros launched '''[[MitoFit Preprints]]''' - the preprint server for mitochondrial physiology and bioenergetics<br />
::::::» [[Gnaiger_2019_MitoFit_Preprints_Editorial |'''A vision on preprints''']]<br />
::::::» [[Gnaiger_2019_MitoFit_Preprints |'''Mitochondrial respiratory states and rates''']] : >530 coauthors join forces towards harmonization on nomenclature<br />
::::::» '''[[Gentle Science]]''' - recognizes the responsibility of the scientific community - for the quality of science, the quality of life in science, and its mission</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=O2k-J&diff=246711O2k-J2024-03-29T08:12:46Z<p>Gnaiger Erich: Created page with "{{Oroboros highlights page name}} __TOC__ <br /> == O2k-J == :::: <big>Oroboros - as a driving force in mitochondrial physiology - extends the analytical and diagnostic powe..."</p>
<hr />
<div>{{Oroboros highlights page name}}<br />
<br />
__TOC__<br />
<br /><br />
<br />
== O2k-J ==<br />
:::: <big>Oroboros - as a driving force in mitochondrial physiology - extends the analytical and diagnostic power of high-resolution respirometry by integration of the [[O2k-Fluo Smart-Module]] and the [[PB-Module]] for PhotoBiology. We aim at establishing the Oroboros quality control management to address the acute ''reproducibility crisis'' of scientific investigation, to resolve the ''inflation crisis'' and ultimately the ''value-impact crisis'' of present academic publication. </big><br />
<br />
<br />
::::» [[MiPNet28.08 Oroboros O2k Series J manual |Oroboros O2k-J Manual]]<br />
<br />
<br />
== Gentle Science ==<br />
<br />
:::: In addition to O2k-Workshops and on-site training, Oroboros will disseminate the not-for-profit initiatives on Mitochondrial Physiology and Bioenergetics Science Camps, specialized Mitochondrial Physiology Conferences, and Preprint publications:<br />
::::::» Oroboros is a key player in support of the [[MitoEAGLE |'''COST Action MitoEAGLE''']] <br />
::::::» '''[[MitoGlobal]]''' - the world-wide platform for societies and organizations supporting mitochondrial research and medicine<br />
::::::» MitoEAGLE with cooperation by Oroboros launched '''[[MitoFit Preprints]]''' - the preprint server for mitochondrial physiology and bioenergetics<br />
::::::» [[Gnaiger_2019_MitoFit_Preprints_Editorial |'''A vision on preprints''']]<br />
::::::» [[Gnaiger_2019_MitoFit_Preprints |'''Mitochondrial respiratory states and rates''']] : >530 coauthors join forces towards harmonization on nomenclature<br />
::::::» '''[[Gentle Science]]''' - recognizes the responsibility of the scientific community - for the quality of science, the quality of life in science, and its mission<br />
<br />
<br />
{{Template:Keywords: O2k}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Installation_and_startup_support_session_self-study_material&diff=246710Installation and startup support session self-study material2024-03-29T08:04:32Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
__TOC__<br />
<br />
== General information == <br />
:::: To benefit optimally from the remote installation ans startup training sessions, please make yourself familiar with '''Materials for self-study''' in advance.<br />
<br />
== Program ==<br />
::::» [[MiPNet26.07_Installation_and_startup_support_session]] <br />
<br />
== Materials for self-study ==<br />
<br />
=== Part 1.1: OroboPOS service and O2k instrumental set up ===<br />
:::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]] (O2k-H to O2k-I) OR [[MiPNet26.13_NextGen-O2k_manual|O2k-Manual: NextGen-O2k Series XA]] OR [[MiPNet28.07 NextGen-O2k Series XB manual|O2k-Manual: NextGen-O2k Series XB]] OR [[MiPNet28.08 Oroboros O2kJ manual|O2k-Manual: Oroboros O2k-J]]<br />
:::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]] (up to Series I and XA) OR [[MiPNet28.10 SmartPOS-service|O2k-Manual: SmartPOS-service]] (from Series J and XB)<br />
:::* Step 1: [https://www.youtube.com/watch?v=wMDcSypG3O4&feature=youtu.be O2k-Videosupport: OroboPOS] <br />
:::* Step 2: [https://www.youtube.com/watch?v=HC6MLXO08k8&feature=youtu.be O2k-Videosupport: Cathode cleaning] <br />
:::* Step 3: [https://www.youtube.com/watch?v=sDoU69-SLq0&feature=youtu.be O2k-Videosupport: Anode cleaning] <br />
:::* Step 4: [https://www.youtube.com/watch?v=iGafaPa4OyA&feature=youtu.be O2k-Videosupport: Membrane mounting] <br />
:::* Step 5: [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 O2k-Videosupport: Start DatLab 7] (For O2k-A to O2k-I)<br />
:::* Step 6: [https://www.youtube.com/watch?v=pGzb0KfYwTQ&feature=youtu.be O2k-Videosupport: Insert OroboPOS] <br />
:::* Step 7: [https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be O2k-Videosupport: Insert O2k Chamber] <br />
:::* Step 8: [https://www.youtube.com/watch?v=DuaEJc7FBjk&feature=youtu.be O2k-Videosupport: O2k Chamber volume calibration] <br />
<br />
=== Part 1.2: DatLab ===<br />
:::* [[MitoPedia: DatLab]] <br />
:::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]]<br />
<br />
=== Part 2: O<sub>2</sub> calibration and instrumental background ===<br />
<br />
:::* [[Gnaiger 2008 POS]] <br />
:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]] <br />
:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]] <br />
:::* [https://www.youtube.com/watch?v=onOIVpmOEoE O2k-Videosupport: QC1: POS calibration]<br />
:::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Installation_and_startup_support_session_self-study_material&diff=246709Installation and startup support session self-study material2024-03-29T08:02:08Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
__TOC__<br />
<br />
== General information == <br />
:::: To benefit optimally from the remote installation ans startup training sessions, please make yourself familiar with '''Materials for self-study''' in advance.<br />
<br />
== Program ==<br />
::::» [[MiPNet26.07_Installation_and_startup_support_session]] <br />
<br />
== Materials for self-study ==<br />
<br />
=== Part 1.1: OroboPOS service and O2k instrumental set up ===<br />
:::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]] (Series H to I) OR [[MiPNet26.13_NextGen-O2k_manual|O2k-Manual: NextGen-O2k Series XA]] OR [[MiPNet28.07 NextGen-O2k Series XB manual|O2k-Manual: NextGen-O2k Series XB]] OR [[MiPNet28.08 Oroboros O2kJ manual|O2k-Manual: Oroboros O2k-J]]<br />
:::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]] (up to Series I and XA) OR [[MiPNet28.10 SmartPOS-service|O2k-Manual: SmartPOS-service]] (from Series J and XB)<br />
:::* Step 1: [https://www.youtube.com/watch?v=wMDcSypG3O4&feature=youtu.be O2k-Videosupport: OroboPOS] <br />
:::* Step 2: [https://www.youtube.com/watch?v=HC6MLXO08k8&feature=youtu.be O2k-Videosupport: Cathode cleaning] <br />
:::* Step 3: [https://www.youtube.com/watch?v=sDoU69-SLq0&feature=youtu.be O2k-Videosupport: Anode cleaning] <br />
:::* Step 4: [https://www.youtube.com/watch?v=iGafaPa4OyA&feature=youtu.be O2k-Videosupport: Membrane mounting] <br />
:::* Step 5: [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 O2k-Videosupport: Start DatLab 7] (For O2k-A to O2k-I)<br />
:::* Step 6: [https://www.youtube.com/watch?v=pGzb0KfYwTQ&feature=youtu.be O2k-Videosupport: Insert OroboPOS] <br />
:::* Step 7: [https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be O2k-Videosupport: Insert O2k Chamber] <br />
:::* Step 8: [https://www.youtube.com/watch?v=DuaEJc7FBjk&feature=youtu.be O2k-Videosupport: O2k Chamber volume calibration] <br />
<br />
=== Part 1.2: DatLab ===<br />
:::* [[MitoPedia: DatLab]] <br />
:::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]]<br />
<br />
=== Part 2: O<sub>2</sub> calibration and instrumental background ===<br />
<br />
:::* [[Gnaiger 2008 POS]] <br />
:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]] <br />
:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]] <br />
:::* [https://www.youtube.com/watch?v=onOIVpmOEoE O2k-Videosupport: QC1: POS calibration]<br />
:::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=PB-Module&diff=246708PB-Module2024-03-29T07:59:14Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
__TOC__<br />
{{MitoPedia without banner<br />
|abbr=<br />
|description= [[Image:PB-Module.jpg|right|180px]] The '''PB-Module''' has been developed for conducting measurements of [[PhotoBiology]], including [[photosynthesis]]. It consists of the [[PB-Sensor|PB Light Source]] and electronic components which are an integral part of the [[NextGen-O2k]]. Measurements are recorded and evaluated with the DatLab 8 software.<br />
<br />
|info= [https://www.oroboros.at/index.php/product/pb-module/ '''Product details and purchase information''']<br />
}}<br />
== The [[PB-Module]] of the [[NextGen-O2k]] and O2k-J and higher==<br />
{{Template:PB-Module}}<br />
== Additional resources ==<br />
<br />
::::» [[MiPNet26.11 NextGen-O2k: PB-Module]]<br />
::::» [[Went 2021 MitoFit PB]]<br />
== Support ==<br />
[[File:Template NextGen-O2k.jpg|right|400px|link=NextGen-O2k]]<br />
:::: Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.<br />
<br />
{{NextGen-O2k}}<br />
<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Respirometry<br />
}}<br />
{{Labeling<br />
|additional=MitoPedia:NextGen-O2k<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=O2k&diff=246707O2k2024-03-29T07:52:10Z<p>Gnaiger Erich: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=O2k<br />
|description='''O2k''' - [[Oroboros O2k]]: the modular system for [[high-resolution respirometry]].<br />
|info=[https://www.oroboros.at/index.php/o2k-products/ Marketplace: Oroboros O2k]<br />
}}<br />
{{Template:Keywords: O2k}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry, Fluorometry, Spectrophotometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Oroboros_O2k&diff=246706Oroboros O2k2024-03-29T07:50:42Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
__TOC__<br />
{{MitoPedia without banner<br />
|abbr=<br />
|description=[[File:NextGen-O2k All-in-one 2023.jpg|300px|right|NextGen-O2k all-in-one]]<br />
'''Oroboros O2k''' - the modular system for [[high-resolution respirometry]] (HRR) for mitochondria and cell research. Oroboros delivers the O2k-technology for high-resolution respirometry in mitochondria and cell research. The O2k allows the measurement of respiration at controlled oxygen levels, combined with redox biology (NADH and CoQ), ROS production, mitochondrial membrane potential, ATP production, Ca<sup>2+</sup>, or pH. HRR expands to HRPB: High-Resolution PhotoBiology. <br />
<br />
Small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells to living cells and tissues slices. <br />
<br />
The modular O2k-concept is supported by [[DatLab |DatLab]], with high flexibility for extension by add-on [https://www.oroboros.at/index.php/product-category/products/o2k-modules/ O2k-Modules]. All O2k-Modules are supported by the [[NextGen-O2k]]. The [https://www.oroboros.at/index.php/product/q-module/ O2k-Q-Module] and the [https://www.oroboros.at/index.php/product/nadh-module/ O2k-NADH-Module] are exclusively supported by the NextGen-O2k, whereas the O2k-J provides the basis for all other HRR application but cannot be upgraded to the [https://www.oroboros.at/index.php/product/nextgen-o2k-redox/ NextGen-O2k Redox]. The globally tested and trusted high-resolution O2k technology prioritizes both quality and scientific research output in the field of mitochondrial physiology and pathology, extended to PhotoBiology.<br />
|info= [https://www.oroboros.at/index.php/o2k-products/ Purchase information], [[MiPNet14.10 O2k-Top 10 |O2k-Top 10]]<br />
}}<br />
[[File:Questions.jpg|left|40px]]<br />
<br><br />
<br /><br />
<br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F7F8FF"><br />
:::» <span style="font-size:105%; color:#424242">'''The most common applications of the [[Oroboros O2k]]'''</span><br />
<div class="mw-collapsible-content"><br />
The O2k is used in basic and applied research to study the respiration of tissues, cells and mitochondria. Fairly simple to highly complex, comprehensive experimental protocols can be applied to assess either basal mitochondrial characteristics or obtain diagnostic insight into respiratory pathways. Topically, studies using the O2k range from [[O2k-Publications: Comparative MiP;environmental MiP|comparative physiological]] investigations addressing evolutionary and environmental aspects of mitochondrial metabolism over the energetics related to exercise physiology, [[O2k-Publications: Exercise physiology;nutrition;life style|nutrition and life style]], to aspects of clinical interest such as the impact of [[O2k-Publications: Mitochondrial medicine|metabolic diseases]], [[O2k-Publications: Cancer|cancer]], or [[O2k-Publications: Pharmacology;toxicology|pharmacological interventions]].<br />
<br/><br/><br/><br />
::: '''The most prominent applications of the O2k'''<br />
<br />
::::* <span style="color:#424242">'''Studies on exercise physiology;nutrition;life style'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[MiP area::Exercise physiology;nutrition;life style]] |format=count}} [[O2k-Publications: Exercise physiology;nutrition;life style|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Diagnosis of acquired and genetic mitochondrial diseases'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Inherited]] |format=count}} [[O2k-Publications: Mitochondrial disease|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Diagnosis of myopathies and neuromuscular pathologies'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Myopathy]] |format=count}} [[O2k-Publications: Myopathy|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on cell function and cell death - apoptosis and necrosis'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Cell death]] |format=count}} [[O2k-Publications: Cell death|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Measurement of oxidative stress'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Oxidative stress;RONS]] |format=count}} [[O2k-Publications: RONS; Oxidative stress|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on ischemia-reperfusion injury'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Ischemia-reperfusion]] |format=count}} [[O2k-Publications: Ischemia-reperfusion|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on aging and senescence'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Aging;senescence]] |format=count}} [[O2k-Publications: Aging; senescence|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Pharmacological tests'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[MiP area::Pharmacology;toxicology]] |format=count}} [[O2k-Publications: Pharmacology;toxicology|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on Diabetes 2'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Diabetes]] |format=count}} [[O2k-Publications: Diabetes 2|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''For more applications, browse through the {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] |format=count}} [[O2k-Publications:_Topics|O2k-Publications in the MiPMap]].'''</span><br />
</div><br />
</div><br />
<br />
<br /><br />
[[File:NextGen-O2k flyer A4.jpg|right|400px]]<br />
__TOC__<br />
<br />
{{Template:Keywords: O2k}}<br />
<br />
== Oroboros O2k: development and distribution ==<br />
<br />
[[File:Modular system scheme - new Nextgen Logo.png|300px|left]]<br />
::::* The O2k is developed by [[OROBOROS INSTRUMENTS |Oroboros Instruments]] in cooperation with [http://www.wgt.at WGT] and produced in Austria by WGT.<br />
<br />
::::* High-resolution respirometry: HRR is the basis for combined measurement of respiration with redox biology (NADH and CoQ), ROS production, mitochondrial membrane potential, ATP production, Ca<sup>2+</sup>, or pH.<br />
<br />
::::* [[TIP2k-Module|Titration-Injection microPump TIP2k]]: The O2k-TIP2k combination allows operation with programmable titration regimes, feedback control, and at steady-states, yielding a new flexibility in experimental design by combining the technical advantages of closed and open systems approaches. Automatic titrations for kinetics; feedback-controlled instrumental background tests; respiration in a closed or open system, in aerobic-anaerobic transitions or at steady-state oxygen concentrations, covering the entire physiological oxygen range and the extremes of hypoxia.<br />
<br />
::::* O2k-Modules can be combined with the O2k, and can be added any time to extend the O2k according to instrument comparability and application-specific objectives: [[Q-Module|Q]] (Q-redox state), [[NADH-Module|NAD(P)H autofluorescence]] (NADH-redox state], [[PB-Module|PhotoBiology]] (photosynthesis), [[O2k-Fluo Smart-Module| fluorometry]] (H<sub>2</sub>S, ATP, mitochondrial membrane potential, Ca<sup>2+</sup>), [[O2k-TPP+ ISE-Module|potentiometry]] (pH, TPP+ or TPMP+ for mitochondrial membrane potential, Ca<sup>2+</sup>) and [[O2k-NO Amp-Module|amperometry]] (NO, H<sub>2</sub>S).<br />
<br />
::::* Auxiliary O2k-Tools provide extended support in the context of high-resolution respirometry: [[Oxia]] the O2 regime controller for the O2k; [[Microbalance-Set|Microbalance]], [[O2k-Dissection Set]].<br />
<br />
<br />
== What is an Oxygraph and the O2k? ==<br />
[[Image:O2.jpg|left|60px|link=Oxygen|Dioxygen]]<br />
=== Oxygraph: O2 ===<br />
::::* <big>'''O2'''k:</big> The O2k measures oxygen ([[Oxygen |dioxygen, '''O<sub>2</sub>''']]) as a gas dissolved in aqueous solution. <br />
::::* With the O2k, experimental data are displayed not only of oxygen concentration over time; but real-time transformation of these data allows the experimenter to follow '''[[respiration]]''' (oxygen consumption, oxygen flux) of biological and biochemical samples while the experiment is still running.<br />
::::* An Oxygraph is not used to measure oxygen concentration in a tissue, but for high-resolution analysis of respiratory activity of biological or biochemical samples transferred into the experimental chamber. <br />
<br />
<br />
[[File:Pesta 2012 Methods Mol Biol Fig7A.jpg|250px|link=Pesta_2012_Methods_Mol_Biol|Pesta and Gnaiger 2012]]<br />
:::: [[Image:O2k-Protocols.jpg|right|230px|link=Gnaiger_2014_MitoPathways|MitoPathways]]Specific [[substrate-uncoupler-inhibitor titration]] (SUIT) protocols are applied in studies of cell respiration or mitochondrial activity. In experiments with living cells, different coupling control states can be studied to evaluate ROUTINE respiratory activity, respiration coupled to ATP production, respiratory excess capacity, residual oxygen consumption, and, by extension of conventional protocols, intactness of cell membranes. In mitochondrial preparations such as isolated mitochondria, permeabilized cells and tissue samples, or homogenized tissue, quantitative information is obtained on selected mitochondrial pathways, particularly of the process of '''[[oxidative phosphorylation]] (OXPHOS)''' in mitochondria and bacteria. Comprehensive OXPHOS analysis leads us beyond the highly informative process of oxygen consumption, when high-resolution respirometry is combined with O2k-MultiSensor modules to simultaneously measure the production of reactive oxygen species or mitochondrial membrane potential, nitric oxide production, acid-base balance or calcium with O2k-FluoRespirometry or ion selective electrodes. In the O2k, these bioenergetic parameters are always evaluated in combination with the measurement of oxygen concentration and respiration.<br />
<br />
<br />
<br />
=== Oroboros O2k: O2k and high-resolution respirometry ===<br />
<br />
::::* <big>O'''2k''':</big> The O2k is the '''2'''<sup>nd</sup> generation of our high-resolution respirometers. The first model was available as of [[OROBOROS 1992|1992]]. Development of the new O'''2k''' started in '''2k''' (2000) and the first O'''2'''k was delivered in [[OROBOROS 2002|'''2'''00'''2''']]. <br />
<br />
::::* <big>O2'''k''':</big> The O2k has two experimental chambers ('''k''': Kammern, German for chambers). [[O2k-chamber |Why 0.5 to 2 mL?]]<br />
<br />
::::* <big>'''Power-O2k''':</big> Several O2k units are operated simultaneously ('''P'''1, '''P'''2, ..) to combine high-resolution respirometry with [[Power-O2k |high-output]].<br />
<br />
<br />
:::: '''High-resolution respirometry''' (HRR) is the result of our long-term expertise in instrumental design, software development, mitochondrial physiology, clinical and pharmacological applications, and [[O2k-Network| world-wide scientific cooperation]]. The innovative O2k-developments provide a system of highest quality and distinguish HRR as a method and concept. <br />
<br />
:::: HRR is defined on the basis of instrumental features of the O2k. The limit of detection of oxygen flux is as low as 1 pmol O<sub>2</sub>.s<sup>-1</sup>.mL<sup>-1</sup>. Oxygen backdiffusion at zero oxygen is <4 pmol.s<sup>-1</sup>.mL<sup>-1</sup>. Oxygen consumption by the polarographic oxygen sensor at air saturation and standard barometric pressure (100 kPa) is 2.7 pmol.s<sup>-1</sup>.mL<sup>-1</sup> (± 0.9 SD; 114 test runs at 37 °C). These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for automatic background correction of oxygen flux (DatLab). Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Signal noise at zero oxygen concentration is <0.05 µM O<sub>2</sub>.<br />
[[File:NextGen O2k + close detail.png|right|530px]]<br />
<br />
:::: The Oroboros O2k is the unique state-of-the-art instrument for HRR - where high-resolution counts: <br />
<br />
::::* with small amounts of cells or tissue, <br />
::::* at low respiratory activities, <br />
::::* multiple substrate-uncoupler-inhibitor titrations (SUIT protocols) with resolution of changes in oxygen flux over prolonged incubation times,<br />
::::* flexibility of O2k-MultiSensor applications,<br />
::::* fast transitions at low oxygen levels,<br />
::::* stability and quality control. <br />
<br />
:::: Today, such high resolution meets rapidly increasing demands, reflected by the increasing number and high level of [[O2k-Publications]]. Applications of the O2k in leading [[O2k-Network| O2k-Network Labs]] are the driving forces for a rapid advancement of mitochondrial physiology. Open access to the [[O2k-Manual]] is an integral part of the concept of [[OROBOROS INSTRUMENTS |Oroboros Instruments]] on [[Gentle Science]] and Scientific Social Responsibility. With sharing of [[O2k-Procedures]] and implementation of quality control, the study of mitochondrial and cell respiration emerges as a quantitative science. Thus high-resolution respirometry provides the foundation for comparative mitochondrial physiology, biomedical research, translation into clinical applications, establishing quantitative functional mitochondrial diagnosis. <br />
<br />
::::* Sole source information: [http://www.bioblast.at/images/6/60/Oroboros_SoleSource.pdf '''pdf''']<br />
::::* [[Oroboros FAQ|Frequently Asked Questions]] <br />
<br />
:::: The Oroboros O2k sets a world-wide standard in high-resolution respirometry - for mitochondrial studies in cell physiology and pathology. <br />
<br />
:::: In contrast to merely offering an oxygen measuring device, the Oroboros O2k is integrated into a systems approach to high-resolution respirometry. This spans from experimental tools which are invaluable in the lab (e.g. [[ISS]]), or little aids that are crucial for tissue preparation (forceps), mitochondrial respiration medium ([[MiR05-Kit]]), [[O2k-Procedures| substrate-uncoupler-inhibitor titration protocols]], and the unique [[O2k-Workshops]].<br />
<br />
{{Template:Keywords: O2k}}<br />
<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=MitoPedia:_O2k_hardware&diff=246705MitoPedia: O2k hardware2024-03-29T07:48:49Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}{{MitoPedia_navigation_line}}<br />
::: '''MitoPedia: O2k hardware''' is a component of [[O2k-Open Support]].<br />
::: It lists hardware-related topics and the O2k-Modules and main components of the O2k system.<br />
<br />
{{Template:Keywords: O2k}}<br />
<br />
::: ''See also''<br />
::::» '''Complete list of O2k components''' [https://www.oroboros.at/index.php/o2k-products/ O2k-Products]<br />
<br />
<br />
{{#ask:[[MitoPedia O2k and high-resolution respirometry::O2k hardware]]<br />
| mainlabel=Term<br />
|?Has abbr=Abbreviation<br />
|?Description=Description<br />
|format=broadtable<br />
|limit=1000<br />
|order=ascending<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Category:NextGen-O2k&diff=246704Category:NextGen-O2k2024-03-29T07:47:57Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Oroboros highlights page name}}<br />
<big><big>'''The revolutionary all-in-one instrument to conquer mitochondrial disease'''</big></big><br />
<br />
{{Template:Keywords: O2k}}<br />
<br />
<br /><br />
{{NextGen-O2k}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=O2k-FluoRespirometer&diff=246703O2k-FluoRespirometer2024-03-29T07:46:26Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
__TOC__<br />
{{MitoPedia without banner<br />
|abbr=<br />
|description=[[Image:O2k-Fluorometer.jpg|200px|right|O2k-FluoRespirometer]] The Oroboros '''O2k-FluoRespirometer''' - the experimental system complete for [[high-resolution respirometry]] (HRR), including fluorometry, the [[TIP2k-Module |TIP2k]] and the [[O2k-sV-Module]] allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca<sup>2+</sup> (CaG) or ATP production (MgG). <br />
<br />
The '''O2k-FluoRespirometer''' supports all add-on [[O2k-Catalogue: O2k-Modules |O2k-Modules]]: [[O2k-TPP+ ISE-Module]], [[O2k-pH ISE-Module]], [[O2k-NO Amp-Module]], enabling measurement of mt-membrane potential with ion sensitive electrodes (ISE for TPP<sup>+</sup> or TPMP<sup>+</sup>) or pH.<br />
<br />
|info= [https://www.oroboros.at/index.php/product/o2k-fluorespirometer/ '''Product details and purchase information''']<br />
}}<br />
<br />
{{Template:Keywords: O2k}}<br />
<br />
== '''O2k-Series H and I: Features''' ==<br />
[[Image:O2k-FluoRespirometer Concept.jpg|600px|right|O2k-FluoRespirometer]]<br />
<br />
<br />
'''O2k-Series I''' is the latest version of the Oroboros O2k for high-resolution respirometry and O2k-MultiSensor applications. The O2k-Series I sets a new standard in merging high-end technology and corporate social responsibility.<br />
:* Smart Fluorescence-Sensors of the [[O2k-Fluo Smart-Module]] are pre-calibrated and the calibration value is stored in the sensor just like its individual ID. Upon connecting the sensor, the O2k-FluoRespirometer identifies it and adjusts itself to the pre-set calibration data. This feature is only available if the sensors are used with O2k-Series H or newer.<br />
:* [[MitoPedia: DatLab|DatLab 7]] is the current version of software, with integrated DatLab Protocols, improved traceability, quality control, and many more features.<br />
:* USB-C 2.0 (2 external ports - O2k-Series I only) for ISS Series E power supply and USB A 2.0 (2 external ports,O2k-Series I; 4 for O2k-Series H) for additional USB devices. <br />
:* Increased energy efficiency based on the updated O2k-electronics design: power consumption is 24 W at 37 °C (80 % reduction compared to Series D). Running costs are substantially lower as a result of our general concept for sustainable technology. The maximal power input is 120 W.<br />
<br />
<br />
== Additional resources ==<br />
<br />
::::» [[MiPNet22.11 O2k-FluoRespirometer manual| O2k-FluoRespirometer manual]]<br />
::::» [[O2k-Demo_experiments |O2k-Demo experiments]]<br />
::::» [[MitoPedia: Fluorometry]]<br />
::::» [[MiPNet06.05 Specifications | Sole source information]]<br />
::::» [[Oroboros O2k]]<br />
<br />
[[Image:O2k-Publications.jpg|left|116px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]<br />
'''»[[O2k-Publications: Topics]]'''<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]]<br />
|?Was published in year<br />
|?Has title<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=descending<br />
}}<br />
<br />
[[Image:O2k-Publications.png|left|115px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]<br />
<big>'''O2k-Publications: O2k-FluoRespirometry - Abstracts'''</big><br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]] <br />
|?Was submitted in year<br />
|?Has title<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was submitted in year<br />
|order=descending<br />
}}<br />
<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry, Fluorometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry<br />
}}<br />
{{Labeling<br />
|instruments=O2k-Fluorometer<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=NextGen-O2k_Technical_developments&diff=246702NextGen-O2k Technical developments2024-03-29T07:45:26Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Oroboros highlights page name}}<br />
{{NextGen-O2k header page name logo}}<br />
<br /><br />
<br /><br />
<br /><br />
<big><big><big>'''WP1: Technical developments'''</big></big></big><br />
<br />
<br />
__TOC__<br />
<br />
<br />
== Abstract ==<br />
<br />
:::: In this workpackage the Q- and PB-Modules are developed for the NextGen-O2k. <br />
::::* Development of the Q-Module and PB-Module and integration in the NextGen-O2k<br />
::::* Testing of the NextGen-O2k in collaboration with the [[NextGen-O2k#Partners|Early testers]]<br />
::::* Testing and development of the software for the NextGen-O2k Modules <br />
::::* Integration of the new protocols in the DatLab software<br />
<br />
<br />
== Achievements ==<br />
<br />
::::'''Project Year 1:''' In the first year of the NextGen-O2k project, the new Q- and PB-Modules supported by the new architecture of the upgraded DatLab software have been developed and integrated. Additionally, we have successfully partnered with Key Opinion Leaders to begin testing the Q-Module prototypes. Working closely with our subcontractors WGT and H-Tech, key components such as cyclic voltammetry have been integrated into the system.<br />
::::'''Project Year 2:''' During the second year of the project the testing of the PB-Module by algae KOLs began, while testing of the Q-Module continued. Feedback prompted a change in the PB-Sensor that will allow a wider spectrum of research questions to be addressed. Features of the DatLab 8 software were implemented and tested, and SUIT protocols were integrated.<br />
<br />
== Progress and next steps ==<br />
See » [[Talk:NextGen-O2k Technical developments|Track record]]<br />
<br />
<br/><br />
<gallery mode=default perrow=4 widths="350px" heights="300px"><br />
File:IOC141-Qgroup.jpg|Some of the Key Opinion Leader’s at the [[IOC141]] Sept. 2019<br />
File:Algae 5232.JPG|Shaker (laboratory) for algae <br />
File:Algae 5251.JPG|Setting up our lab for algae culturing activities<br />
|-<br />
</gallery><br />
<br />
{{Template:Keywords: O2k}}<br />
<br />
{{NextGen-O2k}}<br />
<br />
{{Labeling<br />
|additional=MitoPedia:NextGen-O2k<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=NextGen-O2k&diff=246701NextGen-O2k2024-03-29T07:44:05Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Oroboros highlights page name}}<br />
<br />
<big><big>The revolutionary all-in-one instrument to conquer mitochondrial disease</big></big><br />
::::::::::::::::::::::» <big><big>'''[https://www.oroboros.at/index.php/product/nextgen-o2k_all-in-one/ new on the market]'''</big></big><br />
[[File:NextGenO2k logo.jpg|450px|right|NextGen-O2k|link=https://www.oroboros.at/index.php/product/nextgen-o2k_all-in-one/]]<br />
__TOC__<br />
<br /><br />
<br />
== NextGen-O2k: Horizon 2020 Framework Programme==<br />
::::* EIC-SMEInst-2018-2020 — SME instrument phase 2, Grant Agreement No. 859770, Oroboros Instruments<br />
::::* '''Budget''': € 2.35 Million<br />
::::* '''Duration''': 24 months, extended additional 5 months<br />
::::* '''Application''': 2019-01-09; '''Pitch in Brussels''': 2019-02-15; '''Granted''': 2019-03-05; '''Start''': 2019-06-01 '''End''': 2021-10-31<br />
<br />
<br />
== NextGen-O2k publications, preprints, and abstracts ==<br />
{{#ask:[[Instrument and method::NextGen-O2k]]<br />
| mainlabel=Link<br />
|?Has title=Reference<br />
|?Was published in year=Year<br />
|?Has info=View<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Has title<br />
|order=descending<br />
}}<br />
<br />
<br />
== About NextGen-O2k ==<br />
[[File:Top 10 reasons for Oroboros.jpg|400px|right]]<br />
:::: <big>Oroboros - as a driving force in mitochondrial physiology - extends the analytical and diagnostic power of high-resolution respirometry by integration of NADH- and Q-redox monitoring in the '''NextGen-O2k'''. We aim at establishing the Oroboros quality control management for dissemination to our worldwide O2k-Network laboratories. This will become an effective contribution to address the acute ''reproducibility crisis'' of scientific investigation. In the spirit of Open Science and global networking, we will enable data sharing across projects and institutions in an Open Access database on mitochondrial physiology and pathology, to resolve the ''inflation crisis'' and ultimately the ''value-impact crisis'' of present academic publication. This will support key developments in mitochondrial medicine. In addition, we expand our business to algal biotechnology and ecology with the photobiology module of the NextGen-O2k, widening our focus from medicine to environment and climate.</big><br />
<br />
<br />
<br />
=== Aims ===<br />
<br />
[[File:Respiration and photosynthesis.png|400px|right|Respiration and photosynthesis]]<br />
:::: '''<big>Form the O2k to the NextGen-O2k</big>'''<br />
:::: Between 1992 and 1994, Oroboros Instruments released its core technology for '''high-resolution respirometry (HRR)''', the '''Oxygraph-2k''' or '''O2k''', for real-time monitoring of oxygen consumption of mitochondria, tissues, and living cells. Dissolved oxygen is measured using high-resolution polarographic oxygen sensors. Its main advantages are the unique signal stability and resolution of oxygen. HRR is the core technology present in every single of the devices and it will remain the basis of the NextGen-O2k.<br />
:::: In 2012, the updated version of the O2k was launched, the '''O2k-FluoRespirometer'''. The integrated Smart Fluo-Sensors combine fluorescence excitation and detection of defined wavelengths that in combination with fluorophores allow for the measurement of additional mitochondrial parameters, such as membrane potential, H<sub>2</sub>O<sub>2</sub> production, calcium concentration, and ATP production. <br />
:::: The unique '''software DatLab''' has been developed for data acquisition and analysis. DatLab allows the researcher to perform calibrations (including automatic barometric and temperature adjustments), background correction, experimental SUIT (substrate-uncoupler-inhibitor-titration) protocols and data analysis. <br />
<br />
<br />
:::: With the O2k core technology, developed exclusively by Oroboros for high-resolution measurement of oxygen flux, the '''NextGen-O2k''' was developed. <br />
<br />
<br />
[[File:Oroboros products timeline.jpg|400px|right]]<br />
:::: <big>'''O2k'''</big><br />
:::: '''[[High-resolution respirometry]] (HRR)''' and unique combinations of the O2k with<br />
::::* [[Fluorometry]]: the [[O2k-FluoRespirometer]]<br />
::::* [[Potentiometry]]: the [[O2k-pH ISE-Module]] and [[O2k-TPP+ ISE-Module]]<br />
::::* Amperometric measurement of nitric oxide: the [[O2k-NO Amp-Module]] <br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
[[File:NextGen-O2k modules.jpg|400px|right]]<br />
:::: <big>'''NextGen-O2k'''</big><br />
:::: Extend high-resolution respirometry (HRR) to the all-in-one device<br />
<br />
:::: The NextGen-O2k is a MultiSensor all-in-one instrument for in-depth studies of (''1'') the mitochondrial role in human diseases, and (''2'') mitochondria and chloroplasts ability in regulating algal growth and metabolite production rates. <br />
::::* '''(+1)''' Detection of the redox state of the [[Q-junction]]: [[MiPNet24.12 NextGen-O2k: Q-Module]]<br />
::::: '''(+2)''' Controlled wavelengths and light intensity: PhotoBiology-Module<br />
::::* Save on experimental time, reagents and samples<br />
::::* Minimize errors and ensure reproducible and accurate results<br />
::::* Highest oxygen sensitivity (5 nM detection limit)<br />
::::* Exceptional resolution (wide oxygen concentration range, between 0 - 1,000 μM)<br />
::::* Robustness (>10 years lifespan)<br />
<br /><br />
<br />
<br />
=== Objectives ===<br />
:::: The overall goal of this Phase 2 project is to complete the technical and scientific development of the new NextGen-O2k and prepare it for market launch.<br />
<br />
:::: '''WP1'''<br />
::::» [[NextGen-O2k_Technical_developments|Technical developments]] <br />
<br />
:::: '''WP2'''<br />
::::» [[NextGen-O2k Scientific developments|Scientific developments]]<br />
<br />
:::: '''WP3'''<br />
::::» [[NextGen-O2k Documentation|Documentation]]<br />
<br />
:::: '''WP4'''<br />
::::» [[NextGen-O2k Exploitation|Exploitation]] <br />
<br />
:::: '''WP5'''<br />
::::» [[NextGen-O2k Dissemination |Dissemination]] <br />
<br />
:::: '''WP6'''<br />
::::» [[NextGen-O2k Project management|Project management]] <br />
<br />
== Coordinator == <br />
:::: Oroboros Instruments GmbH<br />
<br />
== Network ==<br />
<br />
=== Partners === <br />
:::: Our partners [http://wiki.oroboros.at/index.php/Oroboros_partners WGT-Elektronik] and [http://wiki.oroboros.at/index.php/Oroboros_partners HTech] have been essential in the development of the NextGen-O2k.<br />
<br />
:::: [[File:WGT Logo 2012.jpg|200px|right|link=http://wiki.oroboros.at/index.php/Oroboros_partners]] [[File:Shtech.png|200px|right|link=http://wiki.oroboros.at/index.php/Oroboros_partners]]<br />
<br />
<br />
=== Early testers === <br />
<br />
:::: The influence of KOLs and Early testers/adopters will be used to build awareness of NextGen-O2k among researchers, academic and industrial partners. We have confirmed relevant mitochondrial and algal biotechnology researchers who want to collaborate as early testers: <br />
<br />
<br />
:::: {| class="wikitable"<br />
|-<br />
! style="width: 40em"|Biomedical research<br />
! style="width: 40em"|Algal research <br />
|-<br />
|[[Zeviani M| Prof. Massimo Zeviani]] <sup>a,b</sup>, [[Viscomi C| Dr. Carlo Viscomi]] <sup>c</sup>, Prof. Mervyn Singer <sup>a</sup> <br><br />
<sup>a</sup> Medical Research Council - Mitochondrial Biology Unit, University of Cambridge, UK<br><br />
<sup>b</sup> Department of Neurosciences, University of Padova, IT<br><br />
<sup>c</sup> Department of Biomedical Sciences, University of Padova, IT<br />
| [[Morosinotto Tomas|Dr. Tomas Morosinotto]]<br><br />
Department of Biology, University of Padova, IT<br />
|-<br />
|[[Garcia-Roves PM| Prof. Pablo Garcia-Roves]]<br><br />
<br />
Department of Physiological Sciences, Faculty of Medicine and Health Sciences, University of Barcelona and Bellvitge Biomedical Research Institute (IDIBELL), ES<br />
<br />
| [[Allahverdiyeva-Rinne Yagut|Prof. Yagut Allahverdiyeva-Rinne]]<br><br />
<br />
Faculty of Technology, University of Turku, FI<br />
|-<br />
|[[Molina AJA|Dr. Anthony Molina]]<br><br />
<br />
Division of Geriatrics and Gerontology, Department of Medicine, University of San Diego, US<br />
| [[Remacle Claire|Prof. Claire Remacle]]<br><br />
<br />
Genetics and Physiology of Microalgae, InBios/Phytosystems Research Unit, University of Liege, BE<br />
|-<br />
|[[Szibor M| Dr. Marten Szibor]]<br><br />
<br />
Clinic for Heart and Thorax Surgery, Jena University Hospital, DE<br />
|<br />
|-<br />
|[[Moore Anthony L| Prof. Anthony Moore]]<br><br />
<br />
Biochemistry and Medicine, School of Life Sciences, University of Sussex, UK <br />
|<br />
|-<br />
|[[Chinopoulos Christos| Dr. Christos Chinopoulos]]<br><br />
<br />
Department of Medical Biochemistry, Semmelweis University, HU <br />
|<br />
|}<br />
<br />
== NextGen-O2k events ==<br />
{{#ask:[[Category:Publications]] [[Additional label::NextGen-O2k]]<br />
|?Was published in year=When<br />
|?Has title=Where<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=descending<br />
}}<br />
<br/><br />
<br />
== NextGen-O2k dissemination ==<br />
:::: [[File:ClusterAward2019.jpg|200px|right]]<br />
::: '''Cluster Award 2019'''<br />
:::: The NextGen-O2k was awarded as the best Tyrolean innovation of the year 2019 with the Cluster Award in the category Life Science. The Cluster Award is funded by the [https://www.standort-tirol.at/&switchLocale=en_US Standortagentur Tirol]. <br />
<br />
<br />
::: '''NextGen-O2k press releases'''<br />
::::* [https://www.wko.at/site/tiroler-innovationspreis/oroboros.html NextGen-O2k ermöglicht noch präzisere Echtzeitmessung der Photosynthese in Algen] - WKO online, 2022-08-28<br />
::::* [https://eic.eismea.eu/community/articles/world-health-day-discover-how-early-diagnoses-virtual-platforms-and-ai-are-improving-our World Health Day: Discover how early diagnoses, virtual platforms and AI are improving our wellness] - EIC community stories - online, 2022-04-07<br />
::::* [https://cordis.europa.eu/article/rcn/435819 Technical leap in mitochondrial research and applications] - CORDIS EU research results - online, 2022-03-21<br />
::::* [https://wiki.oroboros.at/images/0/01/Innovationspreis2021_Oroboros.pdf Tiroler Innovationspreis - Nominierter Technische Innovation] - WKO Tirol, 2022-03-02<br />
::::* [https://wirtschaft.tirol/2022/02/08/tiroler-innovationspreis-geht-in-die-naechste-runde/ Tiroler Innovationspreis geht in die nächste Runde], Tiroler Wirtschaft online, 2022-02-11<br />
::::* [[Media:2020-09-24 tt.com Tiroler Forscher- Depression als Art „Burnout“ in den Zellen - Tiroler Tageszeitung Online.pdf| Depression als Art 'Burnout' in den Zellen]] - Tiroler Tageszeitung online, Sep 2020, 2020-09-24<br />
::::* [[Media:Patente Oroboros Tiroler Tageszeitung 2020.07.03.pdf|Rekord bei Patenten, Tirol auf Rang sechs]] - Tiroler Tageszeitung, July 2020, 2020-07-03<br />
::::* [https://wirtschaftszeit.at/shortcuts-detail/article/oroboros-instruments-revolutioniert-zellbiologische-wissenschaft-zur-erforschung-von-krankheiten Oroboros Instrument revolutioniert Zellbiologische Wissenschaft] - www.wirtschaftszeit.at; 2020-03-30<br />
::::* [https://economyaustria.at/wirtschaft/beste-innovationen-aus-tirol Beste Innovationen aus Tirol]] www.economyaustria.at, 2019-12-16<br />
::::* [https://science.apa.at/rubrik/politik_und_wirtschaft/Clusterawards_2019_Ausgezeichnete_Innovationen_aus_Tirol/SCI_20191213_SCI39491352052190374 Clusterawards 2019: Ausgezeichnete Innovationen aus Tirol] science.apa.at, 2019-12-13<br />
::::* [https://tirol.wirtschaftszeit.at/aus-der-region-detail/article/tiroler-unternehmen-fuer-innovationen-mit-clusterawards-2019-ausgezeichnet Tiroler Unternehmen für Innovationen mit Clusterawards 2019 ausgezeichnet] - www.wirtschaftszeit.at; 2019-12-16<br />
::::* [https://www.meinbezirk.at/innsbruck/c-wirtschaft/clusterawards-2019-ausgezeichnete-innovationen-aus-tirol_a3816486#gallery=null Clusterawards 2019: Ausgezeichnete Innovationen aus Tirol] www.meinbezirk.at, 2019-12-13<br />
::::* [[Media:Landeszeitung Land Tirol Sep 2019 S30-31.pdf|Tiroler Forschungsunternehmen startet durch]] - Tiroler Landeszeitung, September 2019, S30-31, 2019-09-23<br />
::::* [[Media:Der Report 2019 Sonderheft Wirtschaftsnachrichten S12-13.pdf|Innovationsförderung Tirol - Oroboros erhält Zuschuss in Millionenhöhe]], Der Report 2019, Sonderheft der Wirtschaftsnachrichten, S12-13, 2019-09-18<br />
::::* [[Media:Dem Leben beim Atmen zusehen derstandard 2019-08-08.pdf|Dem Leben beim Atmen zusehen]] Der Standard, Beilage Forschung spezial, S 13, 2019-08-07.<br />
::::* [https://www.standort-tirol.at/page.cfm?vpath=presse--medien&genericpageid=25853#0 Turbo für Mitochondrienforschung und Oroboros Instruments], Standortagentur Tirol online, 2019-07-29<br />
::::* [https://www.tt.com/wirtschaft/standorttirol/15900182/tiroler-unternehmen-oroboros-erhaelt-1-7-millionen-euro-eu-foerderung Tiroler Unternehmen Oroboros erhält 1.7 Millionen Euro EU-Förderung], tt.com, 2019-07-29<br />
::::* [https://science.apa.at/rubrik/medizin_und_biotech/Turbo_fuer_Mitochondrienforschung_und_Oroboros_Instruments/SCI_20190729_SCI39451352249801536 Turbo für Mitochondrienforschung udn Oroboros Instruments] science.apa.at, online, 2019-07-29<br />
::::* [https://www.meinbezirk.at/innsbruck/c-wirtschaft/17-millionen-euro-foerderung-fuer-oroboros-instruments_a3542406 1.7 Millionen Euro Förderung für Oroboros Instruments] www.meinbezirk.at online, 2019-07-29<br />
::::* [https://www.derbrutkasten.com/oroboros-eu-foerderung/ 1,7 Millionen Euro EU-Flörderung für Tiroler KMU] www.derbrutkasten.com online, 2019-07-29<br />
::::* [https://theworldnews.net/at-news/tiroler-erfindung-macht-in-europa-furore Tiroler Erfindung macht in Europa Furore] theworldnews.net online, 2019-07-29<br />
::::* [https://www.krone.at/1968609 Tiroler Erfindung macht in Europa Furore] www.krone.at online, 2019-07-29<br />
::::* [[Media:Oroboros Tiroler Tageszeitung 2019.07.30.pdf| EUR 1,7 Mio Förderung für Tiroler KMU Oroboros]] Tiroler Tageszeitung, S 16, 2019-07-30<br />
::::* [[Media:Oroboros Kronenzeitung Tirol 2019.07.10.pdf|Tiroler Millionen-Erfindung]] Tiroler Krone, S 22, 2019-07-30<br />
<br /><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242"> '''NextGen-O2k Literature Package''' - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
<br />
=== Q1: Are you satisfied with the material you received ===<br />
:::: The following result emerged from all of the feedback responses received: <br />
::::* Very satisfied: 71%<br />
::::* Satisfied: 29%<br />
::::* Neutral: 0%<br />
::::* Dissatisfied: 0%<br />
::::* Very dissatisfied: 0% <br />
<br />
=== Q2: Feel free to give your feedback ===<br />
:::: ''Thanks to the interesting material. I will read it very carefully.''<br />
:::: ''Very useful! Thank you for sharing your research.''<br />
<br />
<br />
</div><br />
</div><br />
<br /><br />
<br />
== Gentle Science ==<br />
<br />
:::: In addition to O2k-Workshops and on-site training, Oroboros will disseminate the not-for-profit initiatives on Mitochondrial Physiology and Bioenergetics Science Camps, specialized Mitochondrial Physiology Conferences, and Preprint publications:<br />
::::::» Oroboros is a key player in support of the [[MitoEAGLE |'''COST Action MitoEAGLE''']] <br />
::::::» '''[[MitoGlobal]]''' - the world-wide platform for societies and organizations supporting mitochondrial research and medicine<br />
::::::» MitoEAGLE with cooperation by Oroboros launched '''[[MitoFit Preprints]]''' - the preprint server for mitochondrial physiology and bioenergetics<br />
::::::» [[Gnaiger_2019_MitoFit_Preprints_Editorial |'''A vision on preprints''']]<br />
::::::» [[Gnaiger_2019_MitoFit_Preprints |'''Mitochondrial respiratory states and rates''']] : >530 coauthors join forces towards harmonization on nomenclature<br />
::::::» '''[[Gentle Science]]''' - recognizes the responsibility of the scientific community - for the quality of science, the quality of life in science, and its mission<br />
<br />
<br />
<br />
== MitoEAGLE Q-workshop ==<br />
::::* [http://www.mitoeagle.org/index.php/MitoEAGLE_Innsbruck_2018-11-19 Electron supply to the Q-junction: assessment of mitochondrial respiration, H2O2 flux and the redox state of the Q-pool. COST Action MitoEAGLE, Innsbruck, Nov 2018.]<br />
[[Image:Logo MitoFit-OROBOROS.jpg|right|400px|MitoFit]]<br />
<br />
<br />
<br />
== Support ==<br />
[[File:Template NextGen-O2k.jpg|right|400px|link=NextGen-O2k]]<br />
:::: Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.<br />
<br />
{{Template:Keywords: O2k}}<br />
{{NextGen-O2k}}<br />
<br />
{{Labeling<br />
|additional=MitoPedia:NextGen-O2k<br />
}}<br />
[[Category:NextGen-O2k]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=High-resolution_respirometry&diff=246700High-resolution respirometry2024-03-29T07:42:52Z<p>Gnaiger Erich: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=HRR<br />
|description=[[Image:O2k-Fluorometer.jpg|200px|left|O2k-FluoRespirometer]] '''High-resolution respirometry, HRR''', is the state-of-the-art approach in mitochondria and cell research to measure respiration in various types of [[mitochondrial preparations]] and [[living cells]] combined with MultiSensor modules. <br />
<br />
Mitochondrial function and dysfunction have gained increasing interest, reflecting growing awareness of the fact that mitochondria play a pivotal role in human health and disease. HRR combines instrumental accuracy and reliability with the versatility of applicable protocols, allowing practically unlimited addition and combination of substrates, inhibitors, and uncouplers in the [[Oroboros O2k]]. Substrate-uncoupler-inhibitor titration (SUIT) protocols allow the interrogation of numerous mitochondrial pathway and coupling states in a single respirometric assay. Mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory coupling and pathway control patterns. <br />
<br />
The O2k is a sole source technology, with no other available instrument meeting its specifications for high-resolution respirometry. Technologically, HRR is based on the Oroboros O2k, combining optimized chamber design, application of oxygen-tight materials, electrochemical sensors, Peltier-temperature control, and specially developed software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with both, a closed-chamber or open-chamber mode of operation ([[TIP2k-Module|TIP2k]]). Standardized calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background oxygen flux (systemic flux compensation) provide the experimental basis for high accuracy of quantitative results and quality control in HRR. HRR can be extended for MultiSensor analysis by using the [[O2k-Fluo Smart-Module]]. [[Smart Fluo-Sensor]]s are integrated into the O2k to measure simultaneously fluorometric signals using specific fluorophores. Potentiometric modules are available with ion-selective electrodes (pH, TPP<sup>+)</sup>. The [[PB-Module]] extends HRR to PhotoBiology with accurate control of the light intensity and measurement of photosynthesis. The O2k-J and the NextGen-O2k support all these O2k-Modules. The [[NextGen-O2k]] all-in-one, however, is unique in supporting Q-Redox and NADH-Redox Modules.<br />
|info=[[Oroboros O2k]]<br />
}}<br />
<br />
::::* <big>'''Power O2k''':</big> Several O2k units are operated simultaneously ('''P'''1, '''P'''2, ..) to combine high-resolution respirometry with [[Power O2k-Respirometer |high-output]].<br />
<br />
{{Template:Keywords: O2k}}<br />
<br />
== MitoPedia: O2k and high-resolution respirometry ==<br />
::::» [[MitoPedia: O2k hardware]]<br />
::::» [[MitoPedia: DatLab]]<br />
::::» [[MitoPedia: SUIT]]<br />
<br />
<br />
== Links ==<br />
::::'''»''' [[MitoPedia: Respiratory states]] [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ET capacity]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]<br />
::::» [[Respirometry]]<br />
::::» [[MitoPedia: Respirometry]]<br />
::::» [http://en.wikipedia.org/wiki/Respirometry Wikipedia: Respirometry]<br />
::::» [[O2k Applications]]<br />
<br />
<br /><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
:::[[File:Questions.jpg|left|24px |Explanation of terms]] '''» Keywords'''<br />
<div class="mw-collapsible-content"><br />
::: '''Instrument'''<br />
::::» [[DatLab]]; » [[MitoPedia: DatLab]]<br />
::::» Instrumental background oxygen flux »» [[MiPNet14.06 Instrumental O2 background]]<br />
::::» [[Oroboros O2k-Core (O2k-Series D - G)]]<br />
::::» [[O2k-FluoRespirometer]]<br />
::::» [[O2k-MultiSensor]]<br />
::::» [[Oroboros O2k]]; » [[MitoPedia: O2k hardware]]<br />
::::» [[Power O2k-Respirometer]]<br />
::::» [[MiPNet06.03 POS-calibration-SOP |Static sensor calibration]]<br />
::::» [[TIP2k-Module|TIP2k]]<br />
<br />
::: '''Concept'''<br />
::::» [[Concentration]]<br />
::::» [[Coupling-control state]] <br />
::::» [[High-resolution respirometry]]<br />
::::» [[Mitochondrial preparations]]<br />
::::» [[Oxidative phosphorylation]]<br />
::::» [[Oxygen |Oxygen, dioxygen, O<sub>2</sub>]]<br />
::::» [[Oxygen flux]]<br />
::::» [[Electron-transfer-pathway state]]<br />
::::» [[Respiration]]<br />
::::» [[Respirometry]]; » [[MitoPedia: Respirometry]]<br />
::::» [[SUIT| Substrate-uncoupler-inhibitor titration (SUIT) protocols]]; » [[MitoPedia: SUIT]]<br />
</div><br />
</div><br />
<br /><br />
<br />
[[Image:O2k-Publications.jpg|left|116px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]<br />
<br /><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» References'''</span><br />
<div class="mw-collapsible-content"><br />
::: '''O2k-Publications'''<br />
::::* [[O2k-Publications |O2k-Publications: Chronological]]<br />
::::* [[O2k-Publications: Topics]]<br />
<br />
<br />
== Keywords: Oxygen signal ==<br />
{{Template:Keywords: Oxygen signal}}<br />
<br />
<br />
::: '''Selected references'''<br />
::::* [[Gnaiger 1995 J Bioenerg Biomembr]]<br />
::::* [[Gnaiger 2001 Respir Physiol]]<br />
::::* [[Gnaiger 2008 POS]]<br />
::::* [[Gnaiger 2020 BEC MitoPathways]]<br />
</div><br />
</div><br />
<br /><br />
<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry, Fluorometry, Spectrophotometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Respirometry, O2k-FluoRespirometry<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Oroboros_O2k&diff=246699Oroboros O2k2024-03-29T07:41:07Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
__TOC__<br />
{{MitoPedia without banner<br />
|abbr=<br />
|description=[[File:NextGen-O2k All-in-one 2023.jpg|300px|right|NextGen-O2k all-in-one]]<br />
'''Oroboros O2k''' - the modular system for [[high-resolution respirometry]] (HRR) for mitochondria and cell research. Oroboros delivers the O2k-technology for high-resolution respirometry in mitochondria and cell research. The O2k allows the measurement of respiration at controlled oxygen levels, combined with redox biology (NADH and CoQ), ROS production, mitochondrial membrane potential, ATP production, Ca<sup>2+</sup>, or pH. HRR expands to HRPB: High-Resolution PhotoBiology. <br />
<br />
Small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells to living cells and tissues slices. <br />
<br />
The modular O2k-concept is supported by [[DatLab |DatLab]], with high flexibility for extension by add-on [https://www.oroboros.at/index.php/product-category/products/o2k-modules/ O2k-Modules]. All O2k-Modules are supported by the [[NextGen-O2k]]. The [https://www.oroboros.at/index.php/product/q-module/ O2k-Q-Module] and the [https://www.oroboros.at/index.php/product/nadh-module/ O2k-NADH-Module] are exclusively supported by the NextGen-O2k, whereas the O2k-J provides the basis for all other HRR application but cannot be upgraded to the [https://www.oroboros.at/index.php/product/nextgen-o2k-redox/ NextGen-O2k Redox]. The globally tested and trusted high-resolution O2k technology prioritizes both quality and scientific research output in the field of mitochondrial physiology and pathology, extended to PhotoBiology.<br />
|info= [https://www.oroboros.at/index.php/o2k-products/ Purchase information], [[MiPNet14.10 O2k-Top 10 |O2k-Top 10]]<br />
}}<br />
[[File:Questions.jpg|left|40px]]<br />
<br><br />
<br /><br />
<br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F7F8FF"><br />
:::» <span style="font-size:105%; color:#424242">'''The most common applications of the [[Oroboros O2k]]'''</span><br />
<div class="mw-collapsible-content"><br />
The O2k is used in basic and applied research to study the respiration of tissues, cells and mitochondria. Fairly simple to highly complex, comprehensive experimental protocols can be applied to assess either basal mitochondrial characteristics or obtain diagnostic insight into respiratory pathways. Topically, studies using the O2k range from [[O2k-Publications: Comparative MiP;environmental MiP|comparative physiological]] investigations addressing evolutionary and environmental aspects of mitochondrial metabolism over the energetics related to exercise physiology, [[O2k-Publications: Exercise physiology;nutrition;life style|nutrition and life style]], to aspects of clinical interest such as the impact of [[O2k-Publications: Mitochondrial medicine|metabolic diseases]], [[O2k-Publications: Cancer|cancer]], or [[O2k-Publications: Pharmacology;toxicology|pharmacological interventions]].<br />
<br/><br/><br/><br />
::: '''The most prominent applications of the O2k'''<br />
<br />
::::* <span style="color:#424242">'''Studies on exercise physiology;nutrition;life style'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[MiP area::Exercise physiology;nutrition;life style]] |format=count}} [[O2k-Publications: Exercise physiology;nutrition;life style|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Diagnosis of acquired and genetic mitochondrial diseases'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Inherited]] |format=count}} [[O2k-Publications: Mitochondrial disease|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Diagnosis of myopathies and neuromuscular pathologies'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Myopathy]] |format=count}} [[O2k-Publications: Myopathy|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on cell function and cell death - apoptosis and necrosis'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Cell death]] |format=count}} [[O2k-Publications: Cell death|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Measurement of oxidative stress'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Oxidative stress;RONS]] |format=count}} [[O2k-Publications: RONS; Oxidative stress|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on ischemia-reperfusion injury'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Ischemia-reperfusion]] |format=count}} [[O2k-Publications: Ischemia-reperfusion|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on aging and senescence'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Aging;senescence]] |format=count}} [[O2k-Publications: Aging; senescence|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Pharmacological tests'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[MiP area::Pharmacology;toxicology]] |format=count}} [[O2k-Publications: Pharmacology;toxicology|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on Diabetes 2'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Diabetes]] |format=count}} [[O2k-Publications: Diabetes 2|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''For more applications, browse through the {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] |format=count}} [[O2k-Publications:_Topics|O2k-Publications in the MiPMap]].'''</span><br />
</div><br />
</div><br />
<br />
<br /><br />
[[File:NextGen-O2k flyer A4.jpg|right|400px]]<br />
__TOC__<br />
<br />
== Oroboros O2k: development and distribution ==<br />
<br />
[[File:Modular system scheme - new Nextgen Logo.png|300px|left]]<br />
::::* The O2k is developed by [[OROBOROS INSTRUMENTS |Oroboros Instruments]] in cooperation with [http://www.wgt.at WGT] and produced in Austria by WGT.<br />
<br />
::::* High-resolution respirometry: HRR is the basis for combined measurement of respiration with redox biology (NADH and CoQ), ROS production, mitochondrial membrane potential, ATP production, Ca<sup>2+</sup>, or pH.<br />
<br />
::::* [[TIP2k-Module|Titration-Injection microPump TIP2k]]: The O2k-TIP2k combination allows operation with programmable titration regimes, feedback control, and at steady-states, yielding a new flexibility in experimental design by combining the technical advantages of closed and open systems approaches. Automatic titrations for kinetics; feedback-controlled instrumental background tests; respiration in a closed or open system, in aerobic-anaerobic transitions or at steady-state oxygen concentrations, covering the entire physiological oxygen range and the extremes of hypoxia.<br />
<br />
::::* O2k-Modules can be combined with the O2k, and can be added any time to extend the O2k according to instrument comparability and application-specific objectives: [[Q-Module|Q]] (Q-redox state), [[NADH-Module|NAD(P)H autofluorescence]] (NADH-redox state], [[PB-Module|PhotoBiology]] (photosynthesis), [[O2k-Fluo Smart-Module| fluorometry]] (H<sub>2</sub>S, ATP, mitochondrial membrane potential, Ca<sup>2+</sup>), [[O2k-TPP+ ISE-Module|potentiometry]] (pH, TPP+ or TPMP+ for mitochondrial membrane potential, Ca<sup>2+</sup>) and [[O2k-NO Amp-Module|amperometry]] (NO, H<sub>2</sub>S).<br />
<br />
::::* Auxiliary O2k-Tools provide extended support in the context of high-resolution respirometry: [[Oxia]] the O2 regime controller for the O2k; [[Microbalance-Set|Microbalance]], [[O2k-Dissection Set]].<br />
<br />
<br />
== What is an Oxygraph and the O2k? ==<br />
[[Image:O2.jpg|left|60px|link=Oxygen|Dioxygen]]<br />
=== Oxygraph: O2 ===<br />
::::* <big>'''O2'''k:</big> The O2k measures oxygen ([[Oxygen |dioxygen, '''O<sub>2</sub>''']]) as a gas dissolved in aqueous solution. <br />
::::* With the O2k, experimental data are displayed not only of oxygen concentration over time; but real-time transformation of these data allows the experimenter to follow '''[[respiration]]''' (oxygen consumption, oxygen flux) of biological and biochemical samples while the experiment is still running.<br />
::::* An Oxygraph is not used to measure oxygen concentration in a tissue, but for high-resolution analysis of respiratory activity of biological or biochemical samples transferred into the experimental chamber. <br />
<br />
<br />
[[File:Pesta 2012 Methods Mol Biol Fig7A.jpg|250px|link=Pesta_2012_Methods_Mol_Biol|Pesta and Gnaiger 2012]]<br />
:::: [[Image:O2k-Protocols.jpg|right|230px|link=Gnaiger_2014_MitoPathways|MitoPathways]]Specific [[substrate-uncoupler-inhibitor titration]] (SUIT) protocols are applied in studies of cell respiration or mitochondrial activity. In experiments with living cells, different coupling control states can be studied to evaluate ROUTINE respiratory activity, respiration coupled to ATP production, respiratory excess capacity, residual oxygen consumption, and, by extension of conventional protocols, intactness of cell membranes. In mitochondrial preparations such as isolated mitochondria, permeabilized cells and tissue samples, or homogenized tissue, quantitative information is obtained on selected mitochondrial pathways, particularly of the process of '''[[oxidative phosphorylation]] (OXPHOS)''' in mitochondria and bacteria. Comprehensive OXPHOS analysis leads us beyond the highly informative process of oxygen consumption, when high-resolution respirometry is combined with O2k-MultiSensor modules to simultaneously measure the production of reactive oxygen species or mitochondrial membrane potential, nitric oxide production, acid-base balance or calcium with O2k-FluoRespirometry or ion selective electrodes. In the O2k, these bioenergetic parameters are always evaluated in combination with the measurement of oxygen concentration and respiration.<br />
<br />
<br />
<br />
=== Oroboros O2k: O2k and high-resolution respirometry ===<br />
<br />
::::* <big>O'''2k''':</big> The O2k is the '''2'''<sup>nd</sup> generation of our high-resolution respirometers. The first model was available as of [[OROBOROS 1992|1992]]. Development of the new O'''2k''' started in '''2k''' (2000) and the first O'''2'''k was delivered in [[OROBOROS 2002|'''2'''00'''2''']]. <br />
<br />
::::* <big>O2'''k''':</big> The O2k has two experimental chambers ('''k''': Kammern, German for chambers). [[O2k-chamber |Why 0.5 to 2 mL?]]<br />
<br />
::::* <big>'''Power-O2k''':</big> Several O2k units are operated simultaneously ('''P'''1, '''P'''2, ..) to combine high-resolution respirometry with [[Power-O2k |high-output]].<br />
<br />
<br />
:::: '''High-resolution respirometry''' (HRR) is the result of our long-term expertise in instrumental design, software development, mitochondrial physiology, clinical and pharmacological applications, and [[O2k-Network| world-wide scientific cooperation]]. The innovative O2k-developments provide a system of highest quality and distinguish HRR as a method and concept. <br />
<br />
:::: HRR is defined on the basis of instrumental features of the O2k. The limit of detection of oxygen flux is as low as 1 pmol O<sub>2</sub>.s<sup>-1</sup>.mL<sup>-1</sup>. Oxygen backdiffusion at zero oxygen is <4 pmol.s<sup>-1</sup>.mL<sup>-1</sup>. Oxygen consumption by the polarographic oxygen sensor at air saturation and standard barometric pressure (100 kPa) is 2.7 pmol.s<sup>-1</sup>.mL<sup>-1</sup> (± 0.9 SD; 114 test runs at 37 °C). These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for automatic background correction of oxygen flux (DatLab). Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Signal noise at zero oxygen concentration is <0.05 µM O<sub>2</sub>.<br />
[[File:NextGen O2k + close detail.png|right|530px]]<br />
<br />
:::: The Oroboros O2k is the unique state-of-the-art instrument for HRR - where high-resolution counts: <br />
<br />
::::* with small amounts of cells or tissue, <br />
::::* at low respiratory activities, <br />
::::* multiple substrate-uncoupler-inhibitor titrations (SUIT protocols) with resolution of changes in oxygen flux over prolonged incubation times,<br />
::::* flexibility of O2k-MultiSensor applications,<br />
::::* fast transitions at low oxygen levels,<br />
::::* stability and quality control. <br />
<br />
:::: Today, such high resolution meets rapidly increasing demands, reflected by the increasing number and high level of [[O2k-Publications]]. Applications of the O2k in leading [[O2k-Network| O2k-Network Labs]] are the driving forces for a rapid advancement of mitochondrial physiology. Open access to the [[O2k-Manual]] is an integral part of the concept of [[OROBOROS INSTRUMENTS |Oroboros Instruments]] on [[Gentle Science]] and Scientific Social Responsibility. With sharing of [[O2k-Procedures]] and implementation of quality control, the study of mitochondrial and cell respiration emerges as a quantitative science. Thus high-resolution respirometry provides the foundation for comparative mitochondrial physiology, biomedical research, translation into clinical applications, establishing quantitative functional mitochondrial diagnosis. <br />
<br />
::::* Sole source information: [http://www.bioblast.at/images/6/60/Oroboros_SoleSource.pdf '''pdf''']<br />
::::* [[Oroboros FAQ|Frequently Asked Questions]] <br />
<br />
:::: The Oroboros O2k sets a world-wide standard in high-resolution respirometry - for mitochondrial studies in cell physiology and pathology. <br />
<br />
:::: In contrast to merely offering an oxygen measuring device, the Oroboros O2k is integrated into a systems approach to high-resolution respirometry. This spans from experimental tools which are invaluable in the lab (e.g. [[ISS]]), or little aids that are crucial for tissue preparation (forceps), mitochondrial respiration medium ([[MiR05-Kit]]), [[O2k-Procedures| substrate-uncoupler-inhibitor titration protocols]], and the unique [[O2k-Workshops]].<br />
<br />
{{Template:Keywords: O2k}}<br />
<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Template:Keywords:_O2k&diff=246698Template:Keywords: O2k2024-03-29T07:39:57Z<p>Gnaiger Erich: </p>
<hr />
<div><br /><br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242"> '''»Bioblast links: O2k and HRR''' - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
:::: '''O2k'''<br />
::::::» [[Oroboros O2k]]<br />
::::::» [[High-resolution respirometry]]<br />
::::::» [[NextGen-O2k]]<br />
::::::» [[:Category:NextGen-O2k |Category:NextGen-O2k]]<br />
::::::» [[NextGen-O2k Technical developments]]<br />
::::::» [[MitoPedia: O2k hardware]]<br />
<br />
:::: '''Previous'''<br />
::::::» [[O2k-FluoRespirometer]]<br />
<br />
:::: '''Oroboros Marketplace'''<br />
::::::» [https://www.oroboros.at/index.php/o2k-products/ O2k]<br />
<br />
</div><br />
</div><br />
<br /><br />
<br />
[[Category:Keywords]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Template:Keywords:_O2k&diff=246697Template:Keywords: O2k2024-03-29T07:29:07Z<p>Gnaiger Erich: Created page with "<br /> 40px <br /> Click to expand or collaps <div class="toccolours mw-collapsible mw-collapsed"> ::: <span style=..."</p>
<hr />
<div><br /><br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242"> '''»Bioblast links: O2k and HRR''' - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
:::: '''O2k'''<br />
::::::» [[Oroboros O2k]]<br />
::::::» [[High-resolution respirometry]]<br />
::::::» [[NextGen-O2k]]<br />
::::::» [[MitoPedia: O2k hardware]]<br />
<br />
:::: '''Previous'''<br />
::::::» [[O2k-FluoRespirometer]]<br />
<br />
:::: '''Oroboros Marketplace'''<br />
::::::» [https://www.oroboros.at/index.php/o2k-products/ O2k]<br />
<br />
</div><br />
</div><br />
<br /><br />
<br />
[[Category:Keywords]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=O2k&diff=246696O2k2024-03-29T07:18:11Z<p>Gnaiger Erich: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=O2k<br />
|description='''O2k''' - [[Oroboros O2k]]: the modular system for [[high-resolution respirometry]].<br />
|info=[https://www.oroboros.at/index.php/o2k-products/ Marketplace: Oroboros O2k]<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry, Fluorometry, Spectrophotometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=O2k-Fluo_Smart-Module&diff=246695O2k-Fluo Smart-Module2024-03-29T05:14:50Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
__TOC__<br />
{{MitoPedia without banner<br />
|abbr=<br />
|description=<br />
The '''O2k-Fluo Smart-Module''' is an amperometric add-on module to the [[Startup_O2k-Respirometer| O2k-Respirometer]], adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex® UltraRed), ATP production (Magnesium Green™), mt-membrane potential (Safranin, TMRM), Ca<sup>2+</sup> (Calcium Green™), and numerous other applications open for O2k-user innovation. <br />
<br />
::: » [[MiPNet28.09 O2k-Fluo Smart-Module manual]]<br />
<br />
|info= [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ '''Product details and purchase information''']<br />
}}<br />
<gallery heights="150px" mode="nolines" perrow="6" widths="150px"><br />
File:12100-03.jpg|<br />
Image:SmartFluo.png|<br />
</gallery><br />
== Scope of application ==<br />
{{Template:Scope of fluorescence application}}<br />
<br />
<br />
== Select Smart Fluo-Sensors ==<br />
{{Template:Select fluorescence sensor}}<br />
<br />
== Fluorescence dyes and settings ==<br />
{{Template:Fluorescence dyes and settings}}<br />
<br />
== Mounting a filter cap ==<br />
:::* ''See'': [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a filter cap]]<br />
<br />
== Stoppers ==<br />
{{Template:Stoppers for fluorometry}}<br />
<br />
== Connect Smart Fluo-Sensor to O2k ==<br />
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=nlVLXcbBRqs ]] [https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]<br />
:::: Connect the Fluo-Sensor cable to the Fluo plug of the O2k by inserting the male plug of the cable into the female Fluo plug. The red dot on the male plug faces straight upwards. Each Fluo-Sensor can be used on 2-mL O2k-chambers A or B. The blue frame of the chamber window and the Fluo-Sensor are specially designed to align in a specific rotational position with the cable extending horizontally to left (chamber A) and right (chamber B). The Fluo-Sensor is carefully inserted into the window frame and rotated into final position, leaving no gap between window frame and sensor body.<br />
:::: To remove the Fluo-Sensor, carefully pull out the sensor body with slight back and forth rotations. Do not pull on the cable.<br />
<br />
<gallery heights="150px" mode="nolines" perrow="6" widths="150px"><br />
File:Smart_Fluo-Sensor_-_connect_.png|'''Orientation''' for connection<br />
File:Smart_Fluo-Sensor_RIGHT.jpg|'''OK''', fully inserted<br />
File:Smart_Fluo-Sensor_WRONG.jpg|'''WRONG''', not fully inserted<br />
File:Smart_Fluo-Sensor_inserted.jpg|'''Horizontal''' cable routing<br />
</gallery><br />
<br />
== Electronic settings and LED intensity ==<br />
:::* ''See'': [[Fluorescence-Control_Unit#O2k-Guide|Electronic settings and LED intensity]]<br />
<br />
== O2k signal and output ==<br />
::::# [[O2k signals and output#Signal of the O2k-Core and add-on modules |O2k signal]]: The O2k-Fluo Smart-Module is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [Amp]) as the primary signal.<br />
::::# [[O2k signals and output#O2k output |O2k output]]: type I, II, or III, or combinations.<br />
<br />
== DatLab 7.4 ==<br />
:::*''-See:'' In the [[O2k control]] window in the [[Amperometric,Amp]] tab, set fluorescence intensity and amplification of the signal.<br />
:::* ''-See:'' [[Layout_for_DatLab_graphs#Reference_Layouts:_O2_.26_Amp|Layouts]]<br />
<br />
== O2k-Fluorometry and the TIP2k ==<br />
::::* [[Titration-Injection microPump |TIP2k]]: Our tests indicated that the fluorometric signal is not affected by the TIP2k needle inserted into the 2-mL O2k-chamber.<br />
<br />
== Troubleshooting ==<br />
:::* ''-See'': [[Defective fluorescence module]]<br />
== References ==<br />
[[Image:O2k-Publications.jpg|left|116px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» O2k-Publications'''</span><br />
<div class="mw-collapsible-content"><br />
'''»[[O2k-Publications: Topics]]'''<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]]<br />
|?Was published in year<br />
|?Has title<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=descending<br />
}}<br />
</div><br />
</div><br />
<br /><br />
<br />
<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» O2k-Publications: O2k-Fluorometry - Abstracts'''</span><br />
<div class="mw-collapsible-content"><br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]] <br />
|?Was submitted in year<br />
|?Has title<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was submitted in year<br />
|order=descending<br />
}}<br />
</div><br />
</div><br />
<br /><br />
==Additional resources==<br />
::::» [[O2k-FluoRespirometer]]<br />
::::» [[MiPNet22.11 O2k-FluoRespirometer manual]]<br />
::::» [[O2k-Demo_experiments#O2k-Demo_O2k-MultiSensor |O2k-Demo experiments]]<br><br />
::::» [[O2k-Catalogue:_O2k-Modules|O2k-Modules]]<br><br />
::::» [[O2k-Innovation]]<br><br />
<br />
<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::: '''Specific'''<br />
::::» [[O2k-FluoRespirometer]]<br />
::::» [[Amplex red|Amplex® UltraRed]]<br />
::::» [[Calcium green|Calcium Green™]]<br />
::::» [[Magnesium green|Magnesium Green™]]<br />
::::» [[Safranin]]<br />
::::» [[TMRM]]<br />
::::» [[Smart_Fluo-Sensor_Green]]<br />
::::» [[Smart_Fluo-Sensor_Blue]]<br />
<br />
<br />
::: '''General'''<br />
::::» [[High-resolution respirometry]]<br />
::::» [[mt-membrane potential]]<br />
::::» [[O2k-chamber]]<br />
::::» [[O2k-Core]] <br />
::::» [[O2k-Main Unit]]<br />
::::» [[O2k signals and output#O2k output |O2k output]]<br />
::::» [[O2k signals and output#Signal of the O2k-Core and add-on modules |O2k signal]]<br />
::::» [[Oroboros O2k]]<br />
</div><br />
</div><br />
<br /><br />
{{MitoPedia methods<br />
|mitopedia method=Fluorometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-FluoRespirometry<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Harrison_2015_J_Appl_Physiol&diff=246694Harrison 2015 J Appl Physiol2024-03-29T05:13:47Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Publication<br />
|title=Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol 119:1210-8. https://doi.org/10.1152/japplphysiol.00146.2015<br />
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26251509 PMID: 26251509 Open Access]<br />
|authors=Harrison David K, Fasching M, Fontana-Ayoub M, Gnaiger Erich<br />
|year=2015<br />
|journal=J Appl Physiol<br />
|abstract=Mitochondrial control of cellular redox states is a fundamental component of cell signaling in the coordination of core energy metabolism and homeostasis during normoxia and hypoxia. We investigated the relationship between cytochrome redox states and mitochondrial oxygen consumption at steady-state levels of hypoxia in mitochondria isolated from beef and mouse heart (BHimt, MHimt), comparing two species with different cardiac dynamics and local oxygen demands. A low-noise, rapid spectrophotometric system using visible light for the measurement of cytochrome redox states was combined with high-resolution respirometry. Monophasic hyperbolic relations were observed between oxygen consumption, ''J''<sub>O<sub>2</sub></sub>, and oxygen partial pressure, ''p''<sub>O<sub>2</sub></sub>, within the range <1.1 kPa (8.3 mmHg; 13 µM). ''p''<sub>50''j''</sub> (''p''<sub>O<sub>2</sub></sub> at 0.5∙''J''<sub>max</sub>) was 0.015±0.0004 and 0.021±0.003 kPa (0.11 and 0.16 mmHg) for BHimt and MHimt, respectively. Maximum oxygen consumption, ''J''<sub>max</sub>, was measured at saturating ADP levels (OXPHOS capacity) with Complex I-linked substrate supply. Redox states of cytochromes ''aa''<sub>3</sub> and ''c'' were biphasic hyperbolic functions of ''p''<sub>O<sub>2</sub></sub>. The relation between cytochrome oxidation state and oxygen consumption revealed a separation of distinct phases from mild to severe and deep hypoxia. When cytochrome ''c'' oxidation increased from fully reduced to 45 % oxidised at 0.1 ''J''<sub>max</sub>, ''p''<sub>O<sub>2</sub></sub> was as low as 0.002 kPa (0.02 µM), and trace amounts of oxygen are sufficient to partially oxidize the cytochromes. At higher ''p''<sub>O<sub>2</sub></sub> under severe hypoxia, respiration increases steeply while redox changes are small. Under mild hypoxia, the steep slope of oxidation of cytochrome ''c'' when flux remains more stable represents a cushioning mechanism maintaining respiration high at the onset of hypoxia.<br />
|editor=[[Gnaiger E]]<br />
|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros<br />
}}<br />
::::* This publication presents results obtained with a test prototype instrument of the [[O2k-Innovation]].<br />
<br />
[[Image:Logo MitoFit.jpg|right|120px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]]<br />
== MitoFit news 2015#14 ==<br />
::::* 2015-09-02: Defining mt-hypoxia at cytochrome redox steady-states with the [[O2k-Innovation]]. » [[K-Regio_MitoFit#2015 |MitoFit news]]<br />
<br />
== Cited by ==<br />
::* 8 articles in PubMed (2022-05-30) https://pubmed.ncbi.nlm.nih.gov/26251509/<br />
<br />
{{Labeling<br />
|area=Respiration, Instruments;methods, Comparative MiP;environmental MiP<br />
|injuries=Hypoxia<br />
|organism=Mouse, Bovines<br />
|tissues=Heart<br />
|preparations=Isolated mitochondria<br />
|topics=Oxygen kinetics, Redox state<br />
|couplingstates=OXPHOS<br />
|pathways=N<br />
|instruments=Oxygraph-2k, TIP2k, O2k-Spectrophotometer<br />
|additional=MitoFitPublication, Steady state, Tissue normoxia,<br />
MitoFit2022Hypoxia, MitoFit2022rTCA<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=O2k_signals_and_output&diff=246693O2k signals and output2024-03-29T05:12:42Z<p>Gnaiger Erich: </p>
<hr />
<div>{{MitoPedia<br />
|description=Three electronic '''channel types''' are available in the [[O2k-MultiSensor |O2k-MultiSensor system]]. All channels are available twofold (dual-data), for O2k-Chambers A (left) and B (right), based on numerical signals sent at a fixed data sampling time interval (default: 2 s; range 0.2 s to >10 s).<br />
|info=[[O2k-Catalogue: O2k-MultiSensor]]<br />
}}<br />
__TOC__<br />
<br />
== O2k signals ==<br />
[[File:O2k-Core plugs-for-modules.jpg|right|400px|up to O2k Series G]]<br />
[[File:O2k Series H-plugs.JPG|right|380px|O2k Series H]]<br />
<br />
:::: '''1. O<sub>2</sub> channel''' with electric current (ampere [A]) as the primary signal, which is converted to a voltage (volt [V]) the raw signal. The [[O2k-FluoRespirometer]] supports not only the O<sub>2</sub> channel (O2 plug), but also the channel types 2 and 3 for the add-on modules.<br />
<br />
:::: '''2a. Fluo channel''': The Fluo sensor has electric current (ampere [A]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. Therefore, the [[O2k-Fluo LED2-Module]] is connected to the Amp plug (up to O2k-Series G) or the Smart Fluo-Sensors are connected to the separate Fluo plug (O2k-Series H upwards).<br />
<br />
:::: '''2b. Amp channel''': The amperometric channel of the O2k (Amp plug), has electric current (ampere [A]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The Amp channel is used with the [[O2k-NO Amp-Module]]. Up to O2k-Series G, the Amp plug is also used with the [[O2k-Fluo LED2-Module]]. Electronically, 2a and 2b are the same channel.<br />
<br />
:::: '''3. pX channel''' with electric potential (volt [V]) as the primary potentiometric raw signal (BNC pX plug for the ion sensitive electrode, ISE; and separate plug for the pX reference electrode, Ref).<br />
<br />
<br />
'''O2k-Innovation''': additional channel types are available in the [[O2k-Innovation]]:<br />
<br />
:::: '''4. Dual-fluo channel'''.<br />
<br />
:::: '''5. Spectrophotometric channel'''.<br />
<br />
<br />
::: '''System channels''', for instrumental control and monitoring experimental conditions for both O2k-Chamber A and B (single-data). The numberical signals are sent at the same fixed data sampling time interval:<br />
<br />
:::: 6a. [[Block temperature]]: Measured block temperature equivalent to the experimental temperature in the O2k-Chambers at steady state [°C].<br />
<br />
:::: 6b. Peltier power (system channel): Relative heating or cooling power of the Peltier system.<br />
<br />
:::: 6c. [[Barometric pressure]] (system channel): Measured barometric pressure [kPa].<br />
<br />
:::: 6d. Ambient temperature (system channel): Measured temperature of room air (O2k-Series G upwards) [°C].<br />
<br />
:::: 6e. External temperature (system channel): Temperature measured with an independently placed thermistor (O2k-Series G upwards) [°C].<br />
<br />
<br />
::: '''[[Events - DatLab|Events]]''' carry information on a time point during the experiment, a short event name (text), and a longer string of comments (text):<br />
<br />
:::: 7. Events: Automatic events (e.g. from the [[TIP2k]]) and manually triggered events.<br />
<br />
<br />
== O2k output ==<br />
<br />
:::: The 'output' of a channel is the finally important information, which may be summarized in a data base.<br />
<br />
<br />
::: '''Calibrated signals and derived plots''': Three types of output can be derived from the signal of the channels supported by the O2k and displayed as separate plots: <br />
<br />
:::: '''I: Concentration'''. Examples: O<sub>2</sub>; Ca<sup>2+</sup>; [[concentration]] is amount of substance per volume [1 µM = 1 µmol·dm<sup>-3</sup> = 1 nmol·mL<sup>-1</sup>].<br />
<br />
:::: '''II: Flow, flux, rate'''. Examples: O<sub>2</sub> consumption; H<sub>2</sub>O<sub>2</sub> production; volume-specific [[flux]] is the negative or positive time derivative of concentration over time, frequently multiplied by 1,000 [pmol·s<sup>-1</sup>·mL<sup>-1</sup>]. Volume-specific flux normalized for the cell density [10<sup>6</sup>·ml<sup>-1</sup>] is [[flow]] [pmol·s<sup>-1</sup>·mL<sup>-1</sup>]. Volume-specific flux normalized for the mass density of the sample in the O2k-Chamber [mg·mL<sup>-1</sup>] is mass-specific flux [pmol·s<sup>-1</sup>·mg<sup>-1</sup>]. Flux in any respiratory state normalized for flux in a defined reference state is a rate [dimensionless] or [[flux control ratio]].<br />
<br />
:::: '''III: Force, potential'''. Examples: [[TMRM]], [[Safranin]] and [[Rhodamine 123]] for mt-membrane potential; redox potential from redox states of cytochromes or NADH .<br />
<br />
<br /><br />
<br />
<br />
[[File:O2k-Concept.jpg|right|300px|O2k-Concept]]<br />
<br />
::: '''O2k-Modules - applications and output signals'''<br />
<br />
:::: 1. O<sub>2</sub> channel - all O2k configurations: Output type I and II.<br />
<br />
:::: 2a. Fluo (Amp) channel - [[O2k-FluoRespirometer]] and [[O2k-Fluo LED2-Module]]: The focus of the output depends on the specific fluorophore. Output type I, II, or III, or any combination may relate to the primary level of interest.<br />
<br />
:::: 2b. Amp channel - [[O2k-NO Amp-Module]]: Output type I and II.<br />
<br />
:::: 3a. pX channel - [[O2k-TPP+ ISE-Module]]: Output type III.<br />
<br />
:::: 3b. pX channel - [[O2k-pH ISE-Module]]: Output type I and II.<br />
<br />
<br />
<br />
::: '''Additional output types for the O2k-Innovation'''<br />
<br />
:::: 4. Single-fluo channel - [[O2k-Innovation]]: Plots of one selected wavelength can be selected for recording. Output type I(1), II(1), III(1) depends on the fluorophore.<br />
<br />
:::: 5. Dual-fluo channel - [[O2k-Innovation]]: Plots of two selected wavelengths can be selected for simultaneous recording. Output type I(2), II(2), III(2) depends on the fluorophore.<br />
<br />
:::: 6. Spectrophotometric channel - [[O2k-Innovation]]: Output type IV (three plots for redox states of cytochromes ''aa''<sub>3</sub>, ''b'', and ''c'').<br />
<br />
:::: '''IV''': Redox states.<br />
<br /><br />
<br />
:::: '''V''': Pre-calibrated signals for system channels.<br />
<br /><br />
<br />
:::: '''VI''': Events.<br />
<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, DatLab<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=O2k-Innovation&diff=246691O2k-Innovation2024-03-29T05:10:38Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Oroboros highlights page name}} <br />
:::: <big><big>Tyrolean Government grant</big></big><br />
<br />
[[File:Sponsorlogo gefoerdert RGB.jpg|right|350px]]<br />
::::* The project '''O2k-Innovation''' (officially NextGen-O2k project) was supported by the "'''Technologieförderungsprogramm - Tiroler Innovationsförderung'''" of the Tyrolean Government.<br />
::::* '''Project duration''': 2015-02-01 to 2017-01-31<br />
::::* '''[[Partners in the Oroboros Ecosystem]]''': WGT Elektronik, Software Security Networks<br />
::::* '''Application''': 2015-01-30; '''Granted''': 2015-03-24 (total budget € 350.000)<br />
::::* Funding: '''€ 140,000''' plus a bonus of '''€ 5,000''' for the implementation of gender sensitive actions<br />
<br />
<br />
== O2k-Innovation publication ==<br />
<br />
::::* Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol Aug 6:jap.00146.2015. doi: 10.1152/japplphysiol.00146.2015. - [[Harrison_2015_J_Appl_Physiol |»Bioblast link«]]<br />
<br />
<br />
== The fourth dimension of respiratory control ==<br />
<br />
::::# On the "line": one dimension is insufficient.<br />
::::# Coupling and pathway control are two necessarily connected dimensions defining the “area” of respiratory control.<br />
::::# Adding kinetics (including substrate kinetics of O<sub>2</sub>, CHO, adenylates, P<sub>i</sub>, and flux-force kinetics) is the third dimension bringing “volume” into the picture of classical mitochondrial respiratory control.<br />
::::# The O2k-Innovation on top of O2k-MultiSensor modules and the [[O2k-FluoRespirometer]] represent the fourth dimension of respiratory control, by adding analysis of functional variables (such as ROS production, mt-membrane potential, Ca<sup>2+</sup>, proton flux, cytochrome and NADH redox states, ATP production) to the measurement of O<sub>2</sub> and O<sub>2</sub>-flux in the same chamber.<br />
<br />
<br />
== Development of the O2k-Innovation prototype: a look inside ==<br />
<br />
:::: Different stages of prototype development: The O2k-Innovation is becoming more and more packed with two spectrofluorometers, two spectrophotometers, light guides, Peltier temperatue control with new heat pipes, microelectronics.<br />
<br />
<gallery mode=default perrow=2 widths="500px" heights="300px"><br />
File:NextGen 1.JPG<br />
File:NextGen_2.JPG<br />
File:NextGen_4.JPG<br />
File:NextGen_5.JPG<br />
</gallery><br />
<br />
:::: The '''[[NextGen-O2k]]''' builds upon and extends the concept of the O2k-Fluorometer elaborated within the [[MitoCom_O2k-Fluorometer|K-Regio Project ''MitoCom O2k-Fluorometer'']] (2011-2014). To broaden the specifications of the [[O2k-FluoRespirometer]], the NextGen-O2k combines '''[[high-resolution respirometry]]''' with a [[Q-Module]] and [[NADH-Module]] for RedoxBiology and the [[PB-Module]] to study PhotoBiology (including photosynthesis). It is our ultimate goal to further extend the NextGen-O2k with O2k-Spectrofluorometry (including ratiometric measurements), and O2k-Spectrophotometry (allowing for simultaneous measurement of redox states of the cytochromes ''aa''<sub>3</sub>, ''b'', and ''c'').</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Oroboros_track&diff=246690Oroboros track2024-03-29T05:06:15Z<p>Gnaiger Erich: </p>
<hr />
<div>== [[Template:OROBOROS_box 1 |Oroboros ]] ==<br />
<br />
[[Image:Logo OROBOROS INSTRUMENTS.jpg|right|80px|link=Oroboros Instruments|Oroboros]]<br />
* 2015-09-01: [[File:Tube and Syringe Racks with O2k.JPG|240px|link=O2k-Titration Set]] '''New: [[Syringe Racks]]''' & '''[[Tube Racks]]<br />
* 2015-08-29: [[File:Oroboros Ecosystem.jpg|250px|link=About_Oroboros]]<br />
* 2015-07-25: [[File:2015 KrassFitX-MitoFit-team finish.jpg |240px|link=Oroboros MitoFit team KrassFit2015 Kühtai|The Oroboros MitoFit team at the finish line - all together and very wet and well (l to r): Erich, Carolina-AT, Verena L, Carolina-ES, Karolina-PT, Verena E, Georg, Dominik, Ondrej]]<br />
* 2015-03-20: '''[[Oroboros O2k |Oroboros - tested and trusted]]'''<br />
* 2014-12-31: » <big>'''[[MiPNet19.17_O2k_Series_G |O2k-Fluorometer]]'''</big><br />
* 2014-11-19: » '''[[High-resolution respirometry]]''' [[O2k versus multiwell respirometer |makes the difference]]: Reproducibility day by day '''with the [[Oroboros O2k]]'''<br />
* 2014-10-02: » '''Top news: [[MiPNet19.17 O2k Series G| O2k Series G]]'''<br />
* 2014-05-07: » [[Media:MiPNet14.10 O2k-Top10.pdf|Oroboros Top 10]]<br />
[[File:O2k-universe.jpg|200px|right|Oroboros O2k]]<br />
* 2013-10-24: » '''[[O2k-Publications|>1.000 O2k-Publications]]'''<br />
* 2013-08-15: » '''[[O2k-Publications|>950 O2k-Publications]]'''<br />
* 2013-08-15: » '''[[O2k-Publications: Topics]]'''<br />
* 2013-01-25: » [[O2k versus multiwell respirometer|'''O2k''' versus XFe]] - '''why it matters'''<br />
* 2012-10-07: » [[O2k-Feedback]]<br />
* 2012-09-07: » [http://www.ebec2012.uni-freiburg.de/ O2k-Fluo at EBEC 2012 Sep 15-20]<br />
* 2012-01-24: » '''[[Oroboros O2k-Catalogue ]]'''<br />
* 2012-01-24: » [[O2k-Publications]]<br />
* 2011-11-05: » News: [[O2k-Fluo LED2-Module]]<br />
* 2011-09-25: » [[Talk:Rogers 2011 PLoS One|Talk: Cost-effective high-output technology]]<br />
* 2011-02-15: » [[O2k-Publications]]<br />
* 2010-11-10: » [[MiPNet Reference Laboratories]]<br />
* 2010-10-08: » Visit [[Oroboros Instruments]]<br />
<br />
== [[Template:OROBOROS_O2k-Publications_box |O2k-Publications]] ==<br />
<br />
[[Image:O2k-Publications.jpg|left|100px|link=O2k-Publications:_Topics|O2k-Publications in the MiPMap]]<br />
* 2015-01-11: » [[Recent O2k-Publications]]<br />
* 2015-10-28: * Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Differences in mitochondrial function in homogenated samples from healthy and epileptic specific brain tissues revealed by high-resolution respirometry. Mitochondrion doi:10.1016/j.mito.2015.10.007. - [[Burtscher 2015 Mitochondrion|»Bioblast link«]]<br />
* 2015-08-16: Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol 119:1210-8. - [[Harrison 2015 J Appl Physiol |»Bioblast link«]]<br />
* 2015-07-03: High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. [[Makrecka-Kuka 2015 Biomolecules |»Bioblast link«]]<br />
* 2015-04-01: New O2k-topic: [[O2k-Publications: Cryopreservation |Cryopreservation]]<br />
* 2015-02-08: [[O2k-Fluorometer]]<br />
:* Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. »[[Krumschnabel 2015 Methods Mol Biol |PMID 25631019]]«<br />
:* Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »[[Krumschnabel 2014 Methods Enzymol |PMID 24862266]]«<br />
:* Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »[[Chinopoulos 2014 Methods Enzymol |PMID 24862274]]«<br />
:* A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (''Hemiscyllum ocellatum''). J Comp Physiol B 182:91-100. »[[Hickey 2012 J Comp Physiol B |PMID 21748398]]«<br />
<br />
== [[Template:OROBOROS_O2k-Network_box |O2k-Network]] ==<br />
<br />
[[Image:O2k-Network.png|left|100px|link=O2k-Network|O2k-Network]]<br />
* 2015-07-06: '''To the heart of the Amazon''': can mitochondria from fish heart help understanding why human heart mitochondria work the way they do? - An [[MiPNet20.12 IOC103 |O2k-Workshop]] and project with the O2k-Network Lab in Manaus: '''[[BR Manaus Val AL]]'''<br />
* 2015-06-06: The Bengt Saltin Award was presented at '''[[The Saltin Symposium 2015]]''' by HRH Crown Prince Frederik to [[Siebenmann C |Christoph Siebenmann]].<br />
* 2015-05-31: '''Cellular concerns on microwells''': ''But then the whole mix is thrown into a flat-bottomed microwell where the newly sprouted cells bunch up and do not exactly look or behave like cells grown au naturel.'' [[Microwell_respirometry#Are_microwell_cell_cultures_physiological.3F |»''more details''«]]<br />
* 2015-03-29: We welcome Siddiqah George as the '''[[O2k-Network Manager South Africa]]''', cooperating in on-site installations of the O2k and technical support.<br />
* 2015-02-05: The page 'MiPNet Reference Laboratories' / 'O2k-WorldWide' has been re-named as [[O2k-Network]].<br />
[[Image:MitoCom-Kuehtai 3453-web.jpg|right|180px|link=MitoCom_O2k-Fluorometer|MitoCom Tyrol]]<br />
* 2014-12-28: ''MitoCom Tyrol'' - network on recreation, sport and preventive medicine » [[MitoCom_O2k-Fluorometer|altitude training in Kuehtai - 2020 m and more]].<br />
<br />
[[Image:Sunpoint Tsai.JPG|right|138px|link=TW Taoyuan Sunpoint|Sunpoin, Taiwan]]<br />
* 2014-11-24: Following a series of O2k-demo experiments and training sessions ([[IOC98]]), [[TW Taoyuan Sunpoint| '''Sunpoint''']] offers high-level service for the Oroboros O2k in [[MiPNet Laboratories Taiwan|'''Taiwan''']]. Sunpoint collaborates closely with the O2k-Network Lab of [[TW Taoyuan Wang JS| Prof. Jong-Shyan Wang]].<br />
* 2014-10-23: New entries by [[Doerrier C |Carolina Doerrier]]:<br />
:: » [[Oxidative stress]], [[Free radicals]], [[Reactive nitrogen species]], [[Nitric oxide synthase]], [[Melatonin]], [[Cardiolipin]]<br />
* 2014-10-23: New entries by [[Krumschnabel G |Gerhard Krumschnabel]]:<br />
:: » [[Calcium retention capacity]], [[Permeability transition pore]], [[TMRM]] and [[Talk:TMRM]]<br />
* 2014-10-02: New entries by [[Makrecka-Kuka M |Marina Makrecka-Kuka]]:<br />
:: » [[Carnitine acyltransferase]], [[Palmitoyl-CoA]], [[Carnitine palmitoyltransferase I]], [[Carnitine-acylcarnitine translocase]], [[Carnitine acetyltransferase]]<br />
* 2014-08-07: » '''Continuous innovation: [[Cell ergometry]]'''<br />
* 2014-07-19: » [[Krumschnabel 2014 Methods Enzymol|'''mt-Membrane potential with safranin''']]<br />
* '''[[O2k-Publications: Topics |O2k-Publications: {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] |format=count}}]]''' <br />
[[File:HickeyA.jpg|120px|right|link=Talk:O2k_versus_multiwell_respirometer#Tony_Hickey:_Calibration_is_my_chief_concern|Anthony Hickey]]<br />
* 2014-07-10: » Scientific discussion - O2k vs XFe: [[Talk:O2k_versus_multiwell_respirometer#Tony_Hickey:_Calibration_is_my_chief_concern |'''Tony Hickey: Calibration is my chief concern''']]<br />
<br />
* 2014-04-29: » With '''Turkey''', '''Colombia''' and '''Croatia''', the O2k is now applied in [[O2k-Network |'''42 countries''']]. <br />
[[image:O2k-Manual.jpg|left|60px|link=O2k-Manual|O2k-Manual]] 2014-02-25: '''Continuous innovation'''<br />
:: » [[O2k-Manual |New O2k-Manual]]<br />
<br />
* 2014-01-10: » '''SUIT protocols:''' [[Carbonyl cyanide m-chlorophenyl hydrazone |FCCP versus CCCP in uncoupler titrations]]<br />
[[Image:O2k-Publications.jpg|left|80px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]] 2013-10-26: » '''[[O2k-Publications|>1.000 O2k-Publications]]''' <br />
: » [[MitoCom_O2k-Fluorometer|MitoCom]]<br />
* 2013-12-18: » [[2013_Man_in_Extreme_Environments|Man in the Extremes: Born to Run]]<br />
* 2013-10-26: » [[MiPNet18.09 IOC80 Schroecken AT| Two-day training for scientific expeditions: Comparative MiP - Satellite O2k-Workshop, 2013 Oct Schroecken, AT]]<br />
:* [[MiPNet18.13 IOC84 Alaska|Fit for 1000 miles in Alaska]]<br />
:* [[Xtreme Everest 2| Everest Base Camp 2013]]<br />
:* [[Houska Award 2012]]<br />
* 2013-10-24: » '''[[O2k-Network discussion forum]]'''<br />
:: » [[Talk:Oligomycin|Oligomycin]]<br />
* 2013-08-16: » '''[[MiPMap]]s for O2k-Publications''': [[O2k-Publications: Mitochondrial medicine|mt-Medicine]], [[O2k-Publications: Exercise physiology;nutrition;life style|Exercise physiology;nutrition;life style]], [[O2k-Publications: mt-Biogenesis;mt-density|mt-Biogenesis;mt-density]], [[O2k-Publications: Pharmacology;toxicology|Pharmacology]]<br />
[[File:Peter Hochachka Peru 1996.JPG|120px|right|Peter Hochachka, Peru 1996]]<br />
* 2013-08-15: » [[Gnaiger 2013 Abstract MiP2013|Normalization of mitochondrial respiration]]<br />
:: » [[Comparative MiP publications: Species, tissues, cells |'''Comparative mitochondrial physiology''']] - Publications in the [[MiPMap]]<br />
* 2013-07-02: » '''Selection of appropriate methodologies''' - [[Perry 2013 Diabetes|Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.]]<br />
* 2013-05-26: » [[Xtreme Everest 2| Xtreme Everest 2 (2013)]]<br />
:: » [[Bolivia-Mount Chacaltaya 2012|High altitude in Bolivia 2012]]<br />
:: » [[Houska Award 2012]] <br />
* 2013-01-08: » [[O2k-Publications|888 O2k-Publications]] are now also listed according to [[O2k-Publications:_Specific_topics#Tissues_and_Cell_Types|tissues and cell types]]<br />
* 2012-11-13: » '''Hot news''' - [http://www.ncbi.nlm.nih.gov/pubmed/22184011 Dietary capsaicin improves energy metabolism and exercise endurance]<br />
* 2012-09-16: Happy birthday to <br />
:: » [[Drahota Z|Dr. Zdenek Drahota]] - 80 active years - and many more!!<br />
:: » [[Houstek J|Dr. Josef Houstek]] - 65 active years - and many more!!<br />
* 2012-06-30: » [[Mitochondrial Global Network|Do we need a '''Mitochondrial Global Network'''?]]<br />
* 2012-03-23: » [[O2k-Feedback#IOC66_O2k-Fluorometry_Workshop_Innsbruck.2C_March_2012|Feedback]], [[MiPNet17.06 IOC66|IOC66]]<br />
* 2012-03-12: » [[MiPNet17.06 IOC66|IOC66: March 15-16 2012, Austria - First O2k-Fluorometry Workshop]]<br />
* 2011-08-20: » '''[[MitoScore]] - an emerging mt-network'''<br />
* 2010-11-26: » [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]]- Latest News and Programme<br />
* 2010-09-25: » [[Special:BrowseData/Publications|Browse all Publications]]<br />
<br />
== [[Template:OROBOROS_MitoFit_box |K-Regio MitoFit]] ==<br />
* 2015-09-24: » [[Media:PA Standortagentur Tirol K-Regio 2015-09-23.pdf |»download pdf« 2015-09-23 (German)]]<br />
* 2015-09-23: » [[MitoFit Science Camp 2016 Kuehtai AT]]<br />
* 2015-09-18: » ''Breaking news:'' '''[[K-Regio MitoFit]] - a 1.3 Mill € grant received.'''<br />
* 2015-05-28: » [[Microwell respirometry |Blood cells stressed in 96-well plate]]<br />
* 2015-05-06: » [[K-Regio_MitoFit#2015 |MitoFit news]]<br />
* 2015-05-06: » [[K-Regio MitoFit]] - a new perspective<br />
<br />
<br />
== [[Template:OROBOROS_O2k-Workshops_box |O2k-Workshops]] ==<br />
<br />
<big>'''[[Oroboros_Events#Next_O2k-Workshops |Next O2k-Workshops - we bring high-resolution respirometry to you]]'''</big><br />
* 2015-10-28: '''[[MiPNet21.01 IOC Schroecken| O2k-Workshop in Schroecken]] (AT)''', April 2016 <br />
* 2015-06-06: [[MiPNet20.12 IOC103 |Amazon, Manaus IOC103]]: '''To the heart of the Amazon: comparative cardiac mitochondrial physiology of Amazon fish'''. Expedition and O2k-Workshop on HRR and O2k-Fluorometry, Manaus, BR, July 2015.<br />
* 2015-05-12: [[MiPNet20.11 IOC102 Wuhan CN |'''O2k-Workshop IOC102''']]: Wuhan (CN), May 2015 - in cooperation with the Oroboros representative in China: [[CN Shanghai Zenda]]<br />
* 2015-04-09: [[MiPNet20.03 IOC101 | '''101<sup>st</sup> O2k-Workshop''']]: London (UK), April 2015 <br />
*2015-04-01:<br />
: »'''[[MiPNet20.05 IOC| O2k-Workshop Greenville]] (NC, US)''', August 2015 <br />
: »'''[[MiPNet20.10 IOC106 Schroecken| O2k-Workshop Schroecken]] (AT)''', October 2015 <br />
* 2014-11-26: » The [[MiPNet20.02 IOC99 Cape Town|'''99th Oroboros O2k-Workshop''']] will be held in cooperation with the [[ZA_Cape_Town_Ojuka_EO| '''O2k-Network Lab of Prof. Edward O Ojuka''']] in '''Cape Town (ZA)'''<br />
[[File:Schroecken IOC75.JPG|right|250px|Koerbersee - Schroecken, AT]]<br />
[[Image:Gnaiger Erich.jpg|80px|left|link=Gnaiger Erich|Erich Gnaiger]]<br />
The [[MiPNet20.01_IOC100 | '''100<sup>th</sup> O2k-Workshop''']] will be held in [[IOC Schroecken | '''Schroecken - Hotel Koerbersee, Austria''']]<br />
* 2014-05-11: » [[O2k-Workshops in MiPNet Labs]]<br />
* 2014-04-25: » '''IOC90''' at [[MiPNet19.05 IOC90 |'''ISMM2014''' 2014 May 27, Bolzano, IT]]<br />
* 2014-04-05: » IOC91 [[MiPNet19.06 IOC91 |Philadelphia US, 2014 Jun 02-03. Oroboros O2k-Workshop on HRR and O2k-Fluorometry]]. O2k-Network Lab » [[US PA Philadelphia Wallace DC]] <br />
* 2014-01-14: » [[MiPNet18.16 IOC87 Spitsbergen| IOC87: Spitzbergen, Norway - Enjoy the cold]]<br />
* 2013-10-08: » '''80<sup>th</sup> O2k-Workshop: HRR and O2k-Fluorometry''': [[MiPNet18.09 IOC80 Schroecken AT| '''2013 Oct''' Schroecken, AT]]<br />
* 2013-01-25: » '''O2k-Workshop''' [[MiPNet18.04 IOC76| '''2013 May''' Prague, CZ]]<br />
* 2011-05-05: » [[MiPNet16.01 IOC61| IOC61]]<br />
* 2010-11-26: » [[MiPNet15.10_IOC60|60th Workshop on High-Resolution Respirometry (HRR), December 11-16, 2010, in Schröcken, Austria]]<br />
<br />
» [[Oroboros Events |'''All Oroboros Events''']]<br />
<br />
== [[Template:OROBOROS picture of the month| Oroboros picture of the month]] ==<br />
* 2015-09-03: <br />
[[Image:MitoOroboros by Odra Noel.JPG|200px|Mitochondrial Oroboros by Odra Noel]]<br />
<br />
The mitochondrial [[Oroboros symbol |Oroboros]] (by [[Odra Noel]], London, 2012) was a centre piece of [[MiPArt]] at [[Bioblast 2012]] illustrating the cycle of fission and fusion, with quality control as an essential mechanism to stay [[MitoFit]]. Sept 2015.<br />
<br />
* 2015-08-25: <br />
[[File:Ouroboros-2 Stepanova Anna.JPG |240px]]<br />
<br />
An [[Oroboros The Logo |Ouroboros]] (made by [[Stepanova A|Anna Stepanova]], New York, 2015) helping with [[DatLab]] analysis - the picture was sent by Anna Stepanova during her participation in the [[MiPNet20.05 IOC104 Greenville |O2k-Workshop in Greenville, August 2015]].<br />
<br />
<br />
* 2015-07-12:<br />
<br />
[[File:Ouroboros Manaus-Sao Paulo 4631.JPG|200px]]<br />
<br />
An [[Oroboros The Logo |Ouroboros]] tattoo from Sao Paulo walking in Manaus - observed during the [[MiPNet20.12 IOC103 Manaus |O2k-Workshop July 2015]].<br />
<br />
<br />
== 2014 ==<br />
* [[Bioblast alert 2014]]<br />
<br />
=== The most popular O2k-Publication and O2k-Abstract in Bioblast ===<br />
[[Image:O2k-Publications.jpg|left|50px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]<br />
:: > 17,000 views: '''[[Bouillaud 2012 Abstract Bioblast]]'''<br />
:: > 14,000 views: '''[[Pesta 2012 Methods Mol Biol]]'''<br />
:: '''1363''' [[O2k-Publications:_Topics|O2k-Publications in the MiPMap]]<br />
<br />
<br />
[[Image:BB-Bioblast.jpg|left|60px|link=Bioblast:About|Bioblast wiki]]<br />
=== Popular Bioblast pages 2014 ===<br />
{| class="wikitable"<br />
|-<br />
| [[Image:O2k-Info.png|left|30px|link=Oroboros_INSTRUMENTS|Oroboros Instruments]] >70,000 views: '''[[Oroboros Instruments]]'''<br />
<br />
| [[Image:O2k-Network.png|left|25px|link=MiPNet_Reference_Laboratories|O2k-Network Reference Laboratories]] >60,000 views: '''[[Bioblast 2012]]'''<br />
<br />
| [[Image:O2k-Network.png|left|25px|link=MiPNet_Reference_Laboratories|O2k-Network Reference Laboratories]] >50,000 views: '''[[MiPNet Reference Laboratories ]]'''<br />
<br />
|-<br />
| [[Image:O2k-Publications.jpg|left|35px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]] >50,000 views: '''[[O2k-Publications]]'''<br />
<br />
| [[Image:BB-Bioblast.jpg|left|30px|link=Bioblast:About|Bioblast wiki]] >30,000 views: '''[[Gentle Science]]'''<br />
<br />
| [[Image:MitoGlobal.jpg|left|40px|link=Mitochondrial_Global_Network|MitoGlobal]] >20,000 views: '''[[Mitochondrial Global Network]]'''<br />
<br />
|-<br />
| [[Image:MitoPedia.jpg|left|40px|link=MitoPedia|MitoPedia]] >15,000 views: '''[[Uncoupler]]'''<br />
<br />
| [[Image:MitoPedia.jpg|left|40px|link=MitoPedia|MitoPedia]] >10,000 views: '''[[LEAK respiration]]'''<br />
<br />
| [[Image:O2k-Publications.jpg|left|50px|link=O2k-Publications:_Topics|O2k-Publications in the MiPMap]] >5,000 views: '''[[Gnaiger 2000 Proc Natl Acad Sci U S A]]'''<br />
<br />
|-<br />
|}<br />
<br />
== Popular Bioblast pages - current date ==<br />
{{Popular Bioblast pages}}<br />
<br />
[[Talk:Bioblast track |BioblastEditors help]] [[Category:Help]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=K-Regio_MitoFit&diff=246689K-Regio MitoFit2024-03-29T05:05:06Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Oroboros highlights page name}}<br />
:::: <big><big>mitochondria fit & well</big></big><br />
<br />
<br />
2015-05-01 to 2018-10-31<br />
<br />
[[Image:Logo MitoFit-OROBOROS.jpg|right|300px|K-Regio MitoFit]]<br />
'''Diagnosis of mitochondrial functions and mitochondrial injuries'''<br />
:::: Diagnosis of mitochondrial functions and mitochondrial injuries represents a current challenge for biomedical research and development. We address these challenges by introducing and extending the approach of '''[[cell ergometry]]''' as the state-of-the-art functional test of mitochondrial competence. ''MitoFit'' establishes an inter-university Network in Mitochondrial Physiology and Comprehensive Exercise Testing, supporting translational research on mitochondrial competence in close collaboration with the local industry and other networks, e.g. Oncotyrol. '''''Mitochondrial fitness''''' will play an increasing role in therapeutic and preventive medicine, with exercise and caloric balance providing the most effective measures for the reduction of several age-related health risks and degenerative diseases. MitoFit is a gateway and milestone to better diagnose, support and treat patients in our modern, rapidly aging society.<br />
<br />
::::» [[K-Regio MitoFit |K-Regio project MitoFit]]<br />
::::» [[Oroboros Laboratory]]<br />
<br />
__TOC__<br />
== MitoFit - hot topics ==<br />
[[Image:SC2016 (16).JPG|right|300px| Johannes Burtscher, Patrick Schaefer, Luiz Felipe Garcia e Souza, Pablo Garcia-Roves, Enrico Calzia, Stephen Dozier, Ignazio Prieto, Verena Laner, Erich Gnaiger, Devin Manning, Pedro Neves at the MitoFit Science Camp in July 2016 in Kuehtai, Tyrol, Austria]]<br />
<br />
::::» [[MitoFit Science Camp 2016 Kuehtai AT]]<br />
<br />
::::» [[MitoEAGLE |COST Action approved: MitoEAGLE]]<br />
<br />
::::* MitoFit quality control - new on MitoPedia<br />
::::# [[MitoPedia: Sample preparations]]<br />
::::# [[MitoPedia: SUIT]]<br />
::::» [https://www.nia.nih.gov/research/blog/2015/12/making-molecular-map-exercise Making a molecular map of exercise - NIH Common Fund]<br />
<br />
<br />
<br />
<br />
== Concept ==<br />
<br />
:::: A physically active and nutritionally healthy life style induces regulatory mechanisms that maintain aerobic performance and support functional mitochondrial competence. Even in advancing age, exercise increases aerobic fitness and maintains muscle mitochondrial density above the sedentary benchmark, contributing to the high quality of life characteristic of alpine regions such as Tyrol. Functional mitochondrial competence and aerobic fitness reduce several age-related health risks, including cardiovascular and degenerative diseases, such as type 2 diabetes, neurodegenerative diseases (Alzheimer’s, Parkinson’s, Huntington’s), and several types of cancer. As a result of a sedentary life style and progression to obesity, the decline of muscle mitochondrial capacity may contribute to accelerated aging processes and development of degenerative diseases. Diagnostic evaluation of '''functional mitochondrial competence''' requires '''scientific and technological mitochondrial competence'''. '''MitoFit fit&well''' will combine the specialized scientific focus on mitochondrial research and exercise testing with the expertise of local companies of world-wide leadership in this expanding field. Beyond the added value emanating from the combination and coordination of these diagnostic elements, the consortium will collaborate on the implementation of refined and novel diagnostic protocols as a milestone towards an internationally recognized center of excellence, with an explicit focus on sport and clinical perspectives to address mitochondrial health and disease.<br />
[[Image:SC2016 (46).JPG|right|300px|MitoFit Science Camp in July 2016 in Kuehtai, Tyrol, Austria: If muscle mitochondrial adaptations occur in response to high altitude is answered in a presentation by Adam Chicco, one of over 60 participants of the MitoFit Science Camp]]<br />
<br />
=== Objectives ===<br />
<br />
:::: MitoFit combines the expertise of experienced partners in a unique corporate framework. MitoFit will establish a research and diagnostic laboratory network to form a '''Centre of Excellence in Mitochondrial Physiology, Pathology, Comprehensive Exercise Testing and Genetic Fingerprinting'''. Diagnostic analyses include cardiopulmonary (VO2max; CPX) and metabolic (31P-NMR; Ergospect) exercise testing, evaluation of mitochondrial performance (high-resolution respirometry; Oroboros Instruments), and genetic screening (mtDNA haplotypes, relevant nuclear-encoded genes). Refined state-of-the-art diagnostic protocols are extended and evaluated in a training programme under acute high-altitude hypoxia versus normoxic conditions with healthy control subjects. Exposure to acute hypoxia represents a typical condition for millions of individuals annually skiing or mountaineering in Austria and particularly in Tyrol. Comprehensive mitochondrial and exercise testing protocols will be developed and validated in these studies. Case studies will translate the scientific expertise into the clinical setting. As a result, MitoFit will offer and apply these diagnostic services for (i) monitoring of training programmes in competitive athletes, (ii) life style analyses of the general population, (iii) exercise interventions for preventive and therapeutic medicine, and (iv) clinical diagnosis of individual patients. <br />
<br />
=== Technological and scientific innovation ===<br />
<br />
:::: Diagnosis of mitochondrial function has advanced to a new technological level by the introduction of high-resolution respirometry, and on a scientific level of innovation by multiple substrate-uncoupler-inhibitor titration protocols with permeabilized fibres from small muscle biopsies. Nevertheless, compared to the small needle biopsy, functional mt-diagnosis on far less invasive blood samples (thrombocytes) might provide a '''new perspective for large-scale screening''' of individual athletes, extended cohorts in clinical studies, and application for individual patients. Simultaneous measurement of mitochondrial respiration and additional parameters (mt-membrane potential by ion selective electrodes, hydrogen peroxide production by spectrofluorimetry coupled to the O2k) requires the development of new diagnostic protocols that can be applied to permeabilized muscle fibres and/or intact and permeabilized thrombocytes. Although conventional cardiopulmonary exercise testing (CPX) provides insights into the coupling of external to cellular respiration, it mirrors insufficiently the metabolic processes in the contracting and recovering muscle. Thus, combining CPX and techniques assessing bioenergetic processes in the working muscles will undoubtedly increase the conclusiveness of exercise testing in athletes and patients. These diagnostic MultiSensor protocols in combination with comprehensive exercise testing and genetic fingerprinting will generate an '''innovative repertoire of diagnostic tests''' that can be offered in the context of sports and mitochondrial diseases. <br />
<br />
<br />
== From ''MitoCom'' to '''MitoFit''' – 2020 m and above ==<br />
<br />
<br />
[[Image:SC2016 (44).JPG|right|300px|MitoFit Science Camp in July 2016 in Kuehtai, Tyrol, Austria: Martin Burtscher trying to answer the question whether living and exercising at moderate altitude is a health risk or even benefit]]<br />
<br />
:::: Join those who seek the special recreational and adventurous experience with physical exercise in the Alps. Your exercise is the gateway to your mitochondrial fitness.<br />
<br />
:::: '''MitoFit''' evolves as a unique network of practitioners in the high-end sector of recreation, sport and protective medicine, in collaboration with experts on mitochondrial physiology and mitochondrial medicine. <br />
<br />
:::: We all know that feeling of being well from head to toe – or from muscle to brain – up in the mountains. Scientists want to get to the basis of this wellbeing, to gain insights into invisible molecular functions: is it caused by the ’good air‘ we breathe, even though air gets very thin high up in the Alps? How do the lungs, blood, heart, brain, arms and legs – all organs and all somatic cells – react to altitude and relaxation in nature, to challenges in sport or to a short period of life on a mountain pasture?<br />
<br />
:::: Mitochondria are responsible for the energy supply in human and animal tissues; this is [[cell ergometry]], cell respiration – mitochondrial oxygen consumption. With the [[Oroboros O2k]], we determine energy turnover of carbohydrates and fatty acids and quantify the efficiency of mitochondria in maintaining the vital body functions. In order to determine cellular fitness, parameters for oxidative stress and stress resistance are measured along with mitochondrial oxygen consumption. Biomedical research all over the world applies this method for the diagnosis of mitochondrial competence, especially in sports medicine and gerontology. The O2k, developed by the company Oroboros Instruments, is the world-wide leading instrument for diagnosing the aerobic capacity of mitochondria with the help of small samples of tissue or blood. Oroboros Instruments is located in Innsbruck, Austria. In the Mitochondrial Research Laboratory, the company tests the newest developments and develops standard protocols in cell ergometry.<br />
<br />
<br />
<br />
[[Image:MitoCom Tyrol.jpg|left|80px|link=MitoCom O2k-Fluorometer|MitoCom]] <br />
:::: The logo of ''MitoCom Tyrol'' (now transformed to MitoFit) is based on ''MiPArt'' by [[MiPArt 4| Odra Noel]]. ''Further details:'' »[[MitoCom O2k-Fluorometer]].<br />
<br />
<br />
=== Impact on life style: Tyrol – a heart for sport ===<br />
<br />
:::: Mitochondrial knowledge will, with its innovative approach, arouse a new awareness of preventive medicine, to which a physically active and calorie-balanced lifestyle can contribute significantly. Of particular importance is “healthy aging”, a generally improved quality of life and a reduced risk of a multiplicity of degenerative diseases influenced by the mitochondria: e.g. obesity, diabetes 2, various forms of cancer and dementias. Mitochondrial competence will be made a reality to a larger proportion of the population and the regional identity strengthened both amongst our own population and amongst tourists through the offer of exercise-orientated leisure packages in the Alpine landscape of Tyrol. <br />
<br />
[[File:MitoFit-protective medicine.jpg|700px|right|Instrumental innovation and interdisciplinary cooperation in mitochondrial physiology lead to new perspectives of functional mitochondrial diagnosis. Check your mitochondrial health is conveyed to the public as a keyword for taking care of a physically active life style as an invaluable component of preventive and therapeutic medicine.]]<br />
<br />
:::: Linkages are being created by '''MitoFit''' between research and clinical/practical needs with regard to the very recently established Olympic centre at the Institute of Sport Science of the LFU in Innsbruck and the Altitude Training Centre in Kühtai near Innsbruck leading to optimization of diagnostic exercise testing and training strategies. Importantly, this project seeks to promote research on exercise effects at moderate altitude. Tyrol represents a unique location which is visited by several millions of skiers and mountaineers, annually. On the one hand, regular physical activity and probably also exposures to moderate altitudes up to about 2500 m may contribute to well-being and longevity. On the other hand, however, unaccustomed exercise and acute altitude exposure may trigger serious cardiovascular adverse events in subjects at risk. This initiative should contribute to a better understanding of mechanisms responsible for beneficial and adverse effects supporting the development of appropriate preventive measures.<br />
<br />
:::: Appreciation by politicians and communication with an aspirational general public are essential to benefit from world-class research, for comprehensive mitochondrial and exercise testing and implementation of exercise as a medicine, and the development of a scientific foundation that may allow us to translate the complex diagnostic results into a patient-related mitochondrial score. The MitoFit network will emphasize the connections to international mitochondrial networks, for exchange of expertise and standardization of diagnostic approaches, in a joint effort to helping mitochondrial patients, preventing numerous diseases and making a difference to society.<br />
<br />
<br />
[[Image:MitoFit.jpg|left|60px|link=http://www.mitofit.org/index.php/K-Regio MitoFit|K-Regio MitoFit]]<br />
'''See also'''<br />
:::* '''[[K-Regio MitoFit]]''' is the next step following the K-Regio project ''MitoCom'' (mitochondrial competence; [[MitoCom_O2k-Fluorometer|O2k-Fluorometer]]), extending our world-wide technological leadership in mitochondrial respirometry with the development of a quality control system, MitoFit-Knowledge Management Platform and applications in preventive medicine (K-Regio project MitoFit).<br />
:::* Health tourism: http://www.tt.com/wirtschaft/7750785-91/mehr-gesundheit-f%C3%BCr-tirol-urlauber.csp<br />
:::* [http://www.uibk.ac.at/ipoint/dossiers/forschung_im_fokus/983677.html Sport science in focus]<br />
<br />
<br />
== The project MitoFit highlights the benefits of mitochondrial fitness ==<br />
[[Image:MitoFit.jpg|right|150px|link=http://www.mitofit.org/index.php/K-Regio MitoFit|K-Regio MitoFit]] '''* English''' - [[MitoFit_Deutsch| »German]]<br />
<br />
<br />
[[Image:SC2016 (16).JPG|right|300px|MitoFit Science Camp in July 2016 in Kuehtai, Tyrol, Austria: Johannes Burtscher, Patrick Schaefer, Luiz Felipe Garcia e Souza, Pablo Garcia-Roves, Enrico Calzia, Stephen Dozier, Ignazio Prieto, Verena Laner, Erich Gnaiger, Devin Manning, Pedro Neves]]<br />
<br />
=== MitoFit ===<br />
'''MitoFit in health and preventive medicine'''<br />
:::: Physical inactivity is one of the greatest drivers of metabolic dysregulation and preventable diseases in modern societies, and has an impact on ageing populations in particular. It is becoming increasingly apparent that high and robust mitochondrial capacities are central for sustained metabolic health throughout life. Active lifestyles increase mitochondrial capacities and reduce the risk of degenerative diseases. Despite the clear role of high and efficient mitochondrial capacities in preventing metabolic diseases, promoting health and improving life quality in ageing populations, '''there is currently no regimented, quantitative system, or database organised to routinely test, compare and monitor mitochondrial capacities within individuals, populations, or among populations.''' The Oroboros O2k - established in 49 countries - is the only instrument with sufficient stability and reliability for quantitative high-resolution respirometry (HRR). This world-leading technology offers the unique potential for developing the quality control tools required to establish the O2k as a calibrated and standardised ''in vitro'' diagnostic device. Overall, we aim to gain a CE Mark and FDA approval for the O2k, following the innovations on the level of a quality control system, and to transform this high-resolution research instrument into an essential medical device to monitor metabolic health – maintaining mitochondria fit and well to live a healthier, longer life.<br />
<br />
[[Image:SC2016 (49).JPG|right|300px|MitoFit Science Camp in July 2016 in Kuehtai, Tyrol, Austria: MitoFit-training session with Stefan Moser (Sporttherapie Mag. Huber, AT)]]<br />
<br />
=== K-Regio project MitoFit ===<br />
[[Image:Logo MitoFit-OROBOROS.jpg|left|200px|MitoFit]]<br />
[[File:Tirol Logo Standortagentur.jpg|thumb|The project MitoFit is funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.|right|180px]]<br />
:::: '''The K-Regio project MitoFit''' aims at developing novel laboratory standards and diagnostic monitoring of a mitochondrial fitness score. MitoFit will provide a signature to stimulate health tourism in the Alpine setting, introducing a scientific perspective on the benefits of mitochondrial fitness ranging from outdoor sports to quality of life and healthy ageing supported by physiotherapy.<br />
<br />
<br />
::: '''Applicant:''' Medical University of Innsbruck, A.Univ.-Prof. Dr. Erich Gnaiger<br />
<br />
::: '''Application:''' 2015-04-24; '''Granted:''' 2015-09-16<br />
<br />
::: '''Project duration:''' 2015-05-01 to 2018-04-30, extended to 2018-10-31<br />
<br />
::: '''Total budget:''' € 1.26 Mill; financial support by Fonds Tiroler Zukunftsstiftung (LGBl. Nr. 88/1997) € 0.89 Mill.<br />
<br />
<br />
:::: » '''[[MitoFit Science Camp 2016 Kuehtai AT]]'''<br />
:::: » [[MitoFit K-Regio project report]]<br />
<br />
<gallery><br />
Image:Logo MitoFit s.jpg<br />
Image:MUI Logo rz rgb.jpg<br />
Image:Logo LFU.jpg<br />
Image:Logo OROBOROS INSTRUMENTS.jpg<br />
Image:WGT Logo 2012.jpg<br />
Image:Logo HLZ-Kuehtai.png<br />
Image:Logo_Sporttherapie-Huber.png<br />
Image:Tirol Logo Standortagentur.jpg<br />
</gallery><br />
<br />
<br />
=== Concept ===<br />
<br />
:::: MitoFit will provide a unique opportunity within fields of recreation, sports, wellness, lifestyle monitoring and preventive medicine programs. Collaborations with established regional initiatives will leverage on current research initiatives and established expertise in the measurement of mitochondrial function and competence. This will translate to tangible outcomes for the '''MitoFit concept''' as a marketable health, sports performance and medical diagnostic device. The establishment of the MitoFit-Knowledge Management Platform with a database reposited in the '''MitoFit Centre of Excellence''' (Fig. 1) will also provide a keystone to support the leadership of the mitochondrial monitoring instruments developed in translational research on mitochondrial competence (e.g. previous K-Regio project ''MitoCom'') in close collaboration with local industrial partners.<br />
<br />
:::: The O2k-Core and O2k-Fluorometer, which are the gold standard for generating reliable quantitative respirometric data, will be the instruments of choice to build the MitoFit database. Standardized protocols developed by the MitoFit project will be streamlined for applications of the K-Regio project MitoFit. This will lead to a significant expansion of the world-wide market for the O2k, and drive the transition of the O2k from a well established specialized research tool to a standard health and biomedical instrument.<br />
<br />
[[File:MitoFit project structure.jpg|550px|right|thumb|'''Figure 1''': Structure of the MitoFit project. The development of the MitoFit- Knowledge Management Platform as an integrated module of the O2k technology system is in the centre of the project is. Strategies will be developed to pave the way towards obtaining a CE Mark (EU) and gaining regulatory approval by the Food and Drug Administration (FDA, US).<br />
Strategy development for hardware and software will be complemented by innovations in laboratory standards. A quality control system unique in the field of mitochondrial respirometry will be developed, from standardized protocols and sample preparation to the world-wide innovation of a proficiency ring test. These developments will be tested and applied in work packages introducing the MitoFit approach into the fields of lifestyle analyses based on diagnostic assays of mitochondrial function in blood cells.'' ''Health, sport and training programmes will be developed to improve mitochondrial fitness, with a focus on protective medicine, well-being and healthy ageing. The experience gained in implementing laboratory quality controls and during application developments will provide feedback into the structure and function of the MitoFit-Knowledge Management Platform.]]<br />
<br />
<br />
'''Objectives'''<br />
:::: The objective is to achieve the transformation for the O2k from a research instrument to a medical device. The O2k is a high-end product for measuring mitochondrial function by high-resolution respirometry and is currently distributed in 49 countries world-wide. In an increasing number of cases, we are faced with demands on CE, FDA or equivalent approval for clinical diagnosis. Presently, there is no other instrument available on the market which meets such requirements. While continuing our strategy on '''Open innovation''' to maintain our technological leadership in mitochondrial respiratory research, the objective of the present project is to initiate an additional dimension of Strategic innovation towards standardization and comprehensive quality control as required for a medical device.<br />
<br />
'''Technological and scientific innovation'''<br />
::: '''The MitoFit-Knowledge Management Platform (KMP)''' <br />
:::: The MitoFit KMP will be developed as an integral component of the O2k-technology. It will provide the first standardized measures to link human mitochondrial health to physiome data (heart rate, BMI, blood pressure, blood chemistry, ''V''<sub>O2max</sub>). The technological ‘sole source’ status will be systematically secured. An increasing amount of respirometric data on mitochondrial physiology is generated world-wide, enlarging the theoretically available data base at an increasing speed. However, due to lacking standards as to how results are reported, most published data are not available for quantitative meta-analysis.<br />
:::: Even within research groups, data from different projects have to be tediously re-analyzed for quantitative comparison. By extending O2k applications to monitoring personal mitochondrial fitness and diagnosing an increasing range of health conditions, the development of a knowledge management platform will become even more important and mandatory for considerations on CE and FDA approval. The design of the MitoFit-Data Management System will provide the conceptual framework for strategic innovations related to a quality control system for standardization of instrumental quality tests and mitochondrial reference protocols, establishment of reference samples for proficiency tests, and guidelines for data analysis and presentation. Based on a collection of data obtained through this platform, users will be able to search from pre-defined fields or through full-text search for data, documents and a variety of areas of interest. In combination with web-based collaboration tools implemented in the software, the platform will both broaden the actually available data base for all researchers and stimulate the development of research networks.<br />
<br />
::: '''Reference sample of cryopreserved mitochondria''' <br />
:::: The development of a reference sample for respirometry will provide enormous benefits for scientific research and open up new perspectives on clinical applications, enabling a presently unattainable level of quality control in respiratory studies which will (1) increase the scientific potential of on-going and newly initiated mitochondrial studies in basic and applied research. (2) The reference sample will provide the possibility for quality control of quantitative data for direct comparison and evaluation of functional mitochondrial diagnosis on patients monitored over the timecourse of their disease and treatment, and diagnosed by different clinical laboratories. (3) A reference sample can contribute significantly to the substitution and reduction of experiments with sacrificed animals, by (a) replacing animal tissue used for training and routine quality control, or (b) for methodological development, and (c) reducing the number of artefacts in experiments with animal or human tissue by detecting methodological deficiencies in routine quality control using the reference sample.<br />
<br />
::: '''Cryopreservation of human blood cells''' <br />
:::: Obtaining tissue biopsies for the study of mitochondrial function is an invasive approach that requires a clinician to obtain the samples under strictly sterile conditions and that usually goes along with the need to immediately use the samples for measurements. The possibility to measure mitochondrial function in human blood cells will help to overcome these limitations by allowing a far less invasive sampling procedure applicable by anyone proficient in blood drawing and by enabling the collection and storage of samples for later measurements and analysis. It will also allow the repeated sampling of the same individual, as the burden of sampling is dramatically reduced. Altogether, the advantages of using cryopreserved human blood cells for respirometry will immensely widen the applicability of respirometry for the study of human physiology and will provide a significant stimulus for the entire research field.<br />
<br />
::: '''MitoFit proficiency test''' <br />
:::: A proficiency ring test, probing the performance of participating laboratories, will be applied using the reference samples developed in a previous step. Measuring the respiration of reference samples at two pre-defined time points and following strictly defined experimental protocols, it will be possible for the first time to evaluate and compare the proficiency of different labs and to assess the reproducibility of measurements conducted, thereby having a measure of quality control at hand. Based on the outcome of the test, individual labs may set adequate steps to improve their performance and to develop lab SOPs supporting compliance with defined standard requirements.<br />
<br />
'''Current state-of-the-art''' [[File:SUIT.jpg|350px|thumb|'''Figure 2:''' Substrate-uncoupler-inhibitor titration (SUIT) protocols.<br />
<br /><br />
'''A:''' Combined determination of oxygen consumption and H2O2 flux by O2k-Fluorometry in permeabilized HEK cells. Substrate and coupling states are shown in the upper and lower bars. Substrate states are limited to CI- and CI&II-linked respiration. From Makrecka- Kuka et al (2015).<br />
<br /><br />
'''B:''' Coupling/substrate control diagrams for experimental protocol design, including multiple substrate states for fatty acid oxidation, FAO (octanoylcarnitine & malate, OctM), CI-linked (addition of glutamate, G), CI<small>&</small>II-linked (addition of succinate, S), and CII-linked (addition of rotenone, Rot) respiration. Flux control ratios are normalized relative to ETS capacity with convergent CI&II (&FAO) electron input. Arrows 1, 2 and 3 indicate different SUIT protocols with partially overlapping respiratory states. From Gnaiger (2014).]]<br />
<br />
::: '''Knowledge Management Platform''' <br />
:::: Many research groups are working on extensive documentation of genetic, epigenetic, transcriptomic, proteomic, lipidomic and metabolomic datasets in basic and clinical research, including mitochondrial medicine. Relevant techniques include next-generation sequencing to determine the genome (nDNA and mtDNA), methylome, and transcriptome (RNA), and mass spectrometry (proteome, lipidome, K-Regio: MitoFit 4 MitoFit www.mitofit.org metabolome). Such data are available online through various portals, some of which provide Open Access to allow researchers to use the data for addressing their specific interests and working hypotheses.<br />
<br />
:::: The capacity and efficiency of energy transformation through the mitochondrial pathways of core energy metabolism represent key features of the mitochondrial phenotype, but no corresponding data base is available for mapping mitochondrial health versus disease in humans. Performance parameters of OXPHOS are not available in a consistent format for cataloguing and, therefore, are not represented in presently available databases. Quantitative comparability of data generated by different labs on OXPHOS performance is restricted due to different protocols applied for preparing experimental solutions (solution protocols) and lack of standardization of procedure protocols including instrumental performance protocols, mitochondrial and cell preparation protocols and experimental titration protocols (SUIT protocols; Fig. 2).<br />
<br />
:::: With more than 280 and 150 O2k instruments sold in the EU and the US, respectively, it appears that we are not required to obtain a CE Mark or FDA clearance or approval for the O2k. However, this status will change when extending the applications from a research instrument to a medical device intended to report personal mitochondrial fitness disposition results for a wide range of health conditions, including the propensity for degenerative and other complex diseases. According to our knowledge of the available literature, the Oroboros O2k is the only instrument on the world-wide market providing system accuracy criteria compatible with implementation of a Quality Control System for use as an In Vitro Diagnostic (IVD) device.<br />
<br />
<br />
[[File:MitoFit Work packages.jpg|right|400px|MitoFit Workpackages]]<br />
=== Workpackages ===<br />
<br />
::: '''WP1'''<br />
::::» The O2k from research instrument to medical device - [[MitoFit-Medical device]]<br />
<br />
::: '''WP2'''<br />
::::» MitoFit-Knowledge Management Platform - [[MitoFit-KMP]]<br />
<br />
::: '''WP3'''<br />
::::» Laboratory standards for high-resolution respirometry: towards a QCS - [[MitoFit-QCS]]<br />
<br />
::: '''WP4'''<br />
::::» Reference sample: cryopreserved mitochondria - development for functional control assays - [[MitoFit-Reference sample]]<br />
<br />
::: '''WP5'''<br />
::::» Human blood cells as study model of mitochondrial respiration and H2O2 production - [[MitoFit-Bloodcells]]<br />
<br />
::: '''WP6'''<br />
::::» Development of the MitoFit proficiency test - [[Development MitoFit proficiency test|MitoFit-PT]]<br />
<br />
::: '''WP7'''<br />
::::» Development of a sport module: high altitude sprint training - [[MitoFit-Sport module]]<br />
<br />
::: '''WP8'''<br />
::::» Development of a physiotherapy module: effects of physical activity on cardiovascular risk factors in middle-aged subjects - [[MitoFit-Physiotherapy module]]<br />
<br />
::: '''WP9'''<br />
::::» Development of a training module - [[MitoFit-Training module]]<br />
<br />
<br />
=== Partners ===<br />
<br />
[[File:MUI Logo rz rgb.jpg|40px|link=https://www.i-med.ac.at/patienten/ukl_transplant-chirurgie.html|Medical University Innsbruck]] MUI: Universitätsklinik für Visceral-, Transplantations- und Thoraxchirurgie, D. Swarovski Forschungslabor, DSL (Ao. Univ.-Prof. Dr. Erich Gnaiger)<br />
:: https://www.i-med.ac.at/patienten/ukl_transplant-chirurgie.html<br />
:: http://icbi.i-med.ac.at/<br />
[[File:LFU-Innsbruck-logo.png|40px|LF-University Innsbruck]] LFU: Institut für Sportwissenschaften, ISW (Univ.-Prof. DDr. Martin Burtscher, Verena Menz, Dr. Hannes Gatterer)<br />
:: http://www.uibk.ac.at/isw/<br />
[[Image:Logo OROBOROS INSTRUMENTS.jpg|40px|link=OROBOROS INSTRUMENTS|Oroboros]] SME: Oroboros Instruments GmbH, Innsbruck, ORO (Dr. Erich Gnaiger, Mag.a. Verena Laner)<br />
:: http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS<br />
[[File:WGT Logo 2012.jpg |40px |link=http://www.wgt.at|WGT]] SME: WGT-Elektronik GmbH & Co KG, Kolsass, Tyrol, WGT (Philipp Gradl)<br />
:: http://www.wgt.at <br />
[[File:Logo_HLZ-Kuehtai.jpg|40px|link=http://www.hoehentraining-kuehtai.at/|HLZ Kuehtai]] SME: Höhenleistungszentrum Betriebsg.m.b.H., Innsbruck, HLZ (Geschäftsführer Mag. Andreas Brix, Robert Valentini)<br />
:: http://www.hoehentraining-kuehtai.at/<br />
[[File:Logo_Sporttherapie-Huber.png |40px|link=http://www.sporttherapie-huber.at|Sporttherapie Huber]] SME: Sporttherapie Mag. Huber GmbH, Innsbruck, STH (Mag. Reinhard Huber)<br />
:: http://www.sporttherapie-huber.at<br />
<br />
<small> MUI = Medical University Innsbruck; LFU = Leopold-Franzens-University Innsbruck; SME = Small and medium-sized enterprise </small><br />
<br />
'''External consultants'''<br />
:* MUI: Department für Medizinische Statistik, Informatik und Gesundheitsökonomie, MSIG (Univ.-Doz. Dr. Georg Göbel)<br />
:: https://www.i-med.ac.at/msig/<br />
:* software security networks - ssn (Lukas Gradl)<br />
:: http://www.ssn.at/<br />
:* fancy tree films (Johannes Aitzetmüller)<br />
:: http://www.fancytreefilms.com/<br />
:* en.co.tec Schmid KG, Technisches Büro für Elektrotechnik (Dipl.- Ing. Martin Schmid)<br />
:: http://www.encotec.at/<br />
<br />
'''International strategic partners - Europe'''<br />
:* [[Garcia-Roves Pablo M |Garcia-Roves Pablo Maria, PhD Assoc. Prof.]]: Faculty of Medicine, University of Barcelona, ES<br />
:: [[ES Barcelona Garcia-Roves PM]] <br />
:* [[Karabatsiakis A |Karabatsiakis Alexander, PhD]]: Clinical and Biological Psychology, University of Ulm, DE<br />
:: [[DE Ulm Karabatsiakis A]]<br />
:* [[Lundby C |Carsten Lundby, PhD Prof.]]: Institute of Physiology, University of Zurich, CH<br />
:* [[Sumbalova Z |Sumbalova Zuzana, PhD]]: Pharmacobiochemical Laboratory, Faculty of Medicine, Comenius University, Bratislava, SK<br />
:: [[SK Bratislava Sumbalova Z]]<br />
<br />
'''International strategic partners - overseas'''<br />
:* [[Goodpaster BH |Goodpaster Bret H, Prof. Dr]]: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Sanford/Burnham Institute, Orlando, FL, US<br />
:: [[US FL Orlando Goodpaster BH]]<br />
:* [[Hickey AJ |Hickey Anthony, PhD]]: School of Biological Sciences, The University of Auckland, NZ<br />
:: [[NZ Auckland Hickey AJ]]<br />
:* [[Molina AJA |Molina Anthony JA, PhD]]: J. Paul Sticht Center on Aging, Dept Internal Medicine, Wake Forest School of Medicine, Winston-Salem, NC, US<br />
:: [[US NC Winston-Salem Molina AJA]]<br />
:* [[Neufer PD |Neufer P Darrell, Prof. Dr.]] & [[Brown DA| Brown David A, PhD]]: Dept of Exercise and Sport Science & Brody School of Medicine, East Carolina University, Greenville, NC, US<br />
:: [[US NC Greenville Neufer PD]]<br />
:* [[Ojuka EO |Ojuka Edward O, Prof. Dr. PhD]]: Institute of South Africa Newlands, ESSM UCT Dept of Human Biology Sports Science, University of Cape Town, ZA<br />
:: [[ZA Cape Town Ojuka EO]] <br />
<br />
<br />
=== Becoming ‘MitoFit’ – a trend towards a new lifestyle? ===<br />
:::: With its innovative approach, MitoFit arouses a new awareness of preventive medicine, to which a physically active and calorie-balanced lifestyle can contribute significantly. Of particular importance is “healthy aging”, a generally improved quality of life and a reduced risk of a multiplicity of degenerative diseases influenced by the mitochondria: e.g. obesity, diabetes II, various forms of cancer and dementias. Mitochondrial competence will be made a reality to a larger proportion of the population and the regional identity strengthened both amongst our own population and amongst tourists through the offer of exercise-orientated leisure packages in the Alpine landscape of Tyrol.<br />
:::: Making our mitochondria fit – MitoFit and well – may strengthen the trend to a less sedentary, healthier and balanced lifestyle, supported by the growing evidence of the central role of mitochondrial function for an improved and prolonged quality of life.<br />
<br />
<br />
=== Related projects ===<br />
:::# COST Action » [[MitoEAGLE]]<br />
:::# Tiroler Innovationsförderung » [[O2k-Innovation]] (Feb 2015 - Jan 2017)<br />
:::# K-Regio ''MitoCom'': » [[MitoCom_O2k-Fluorometer|MitoCom]] (completed 2014)<br />
<br />
<br />
== MitoFit project news ==<br />
=== 2016 ===<br />
* '''003'''. 2016-05-06:<br />
<br />
: Dear colleague, <br />
<br />
: 1st MitoFit Science Camp, Kuehtai (close to Innsbruck) – 2016, July 07 to 13.<br />
:* Programme update: » [[MitoFit Science Camp 2016 Kuehtai AT]]<br />
:* Abstract deadline: extended to May 27.<br />
:* Early bird registration until May 15: » [[MitoFit SC 2016 Registration]]<br />
<br />
: We are looking forward to seeing you highly MitoFit at 2,020 m in Kuehtai, <br />
: [[Gnaiger Erich| Erich]] and [[Laner Verena| Verena]]<br />
<br />
* '''002'''. 2016-03-30:<br />
<br />
: Dear colleague, <br />
: We are happy to inform you that registration for the 1st MitoFit Science Camp is now open. Immediately following [[EBEC2016]] (July 2-7), the MitoFit Science Camp will take place in Kuehtai (close to Innsbruck), Austria from July 7th to 13th. <br />
:* High-level training in mitochondrial respiratory physiology, <br />
:* Internationally renowned speakers – interlaboratory networking,<br />
:* High-resolution respirometry - mitochondrial performance testing, <br />
:* Discussions on fundamentals of quality control – MitoFit proficiency test<br />
:* Find out more: » [[MitoFit Science Camp 2016 Kuehtai AT]] <br />
: Register now: » [[MitoFit SC 2016 Registration]] <br />
: We are looking forward to seeing you highly MitoFit at 2,020 m in Kuehtai, <br />
<br />
: [[Gnaiger Erich| Erich]] and [[Laner Verena| Verena]]<br />
<br />
* '''001'''. 2016-03-08:<br />
: We are delighted to inform the mitochondrial community about the COST Action 'Mitochondrial mapping: Evolution - Age - Gender - Lifestyle - Environment (MitoEAGLE)', which is going to be funded for four years: <br />
: » [[MitoEAGLE]] <br />
<br />
: Researchers from COST Member Countries and Cooperating States can join the Action. For more information (including Near Neighbour Countries and International Partner Countries) and modalities for joining, see: <br />
: » [[MitoEAGLE network |Join the COST Action CA15203 MitoEAGLE]] <br />
<br />
=== 2015 ===<br />
<br />
* '''020'''. 2015-12-22: Quote from Pedersen and Saltin (2015): ’.. it is now time that the health systems create the necessary infrastructure to ensure that supervised exercise can be prescribed as medicine.’ » [[Pedersen 2015 Scand J Med Sci Sports]] <br />
<br />
:'''MitoFit hot topics:'''<br />
<br />
:MitoFit Workshop on blood cell respirometry for mitochondrial research and diagnostic applications. Innsbruck, 2016-Jan-08:<br />
:* More details » [[MitoFit Workshop Blood Cells 2016-01]] <br />
<br />
:Making a molecular map of exercise - NIH Common Fund:<br />
:* More details » http://www.nia.nih.gov/research/blog/2015/12/making-molecular-map-exercise <br />
<br />
:As the end of the year approaches, we thank you for your positive feedback and cooperation, sending you season's greetings and all the best for a happy, successful, MitoFit and generally healthy New Year.<br />
<br />
* '''019'''. 2015-12-10: From insulin-resistant obese to MitoFit – with aerobic exercise training: » [[Konopka_2015_Diabetes]]<br />
* '''018'''. 2015-11-25: Performance tests on skeletal muscle mitochondria of the Inuit haplogroup reveal fitness information beyond the uncoupling hypothesis for adaptations to the arctic climate: » [[Gnaiger 2015 Scand J Med Sci Sports]]<br />
* '''017'''. 2015-11-16: MitoFit in the brain » [[Burtscher 2015 Mitochondrion]]<br />
[[File:TIP2k-Needle Safety Support overview.JPG|right|100px|link=TIP2k-Module|TIP2k]]<br />
* '''016'''. 2015-10-20: In an effort to examine the comparability of data generated by different laboratories and across various studies, we are taking note of commonly used fatty acids being utilized to examine beta-oxidation.<br />
:Therefore, we would like to ask you the following:<br />
:* What is your experience with examining fatty acid oxidation in permeabilized muscle fibres?<br />
:* Which fatty acids do you use?<br />
:A) Octanoylcarnitine <br />
:B) Palmitylcarnitine <br />
:C) Other (please specify)<br />
<br />
:The results of this survey will be summarized in the O2k-Network discussion forum: » [[O2k-Network discussion forum|O2k-Network discussion forum]]<br />
* '''015'''. 2015-09-24: The '''MitoFit project''' was approved by the Tyrolean Government with a budget of € 1.3 Mill. Our main aim is the development of novel laboratory standards and diagnostic monitoring of a mitochondrial fitness score. The Universities of Innsbruck and industry partners collaborate with international strategic partners in this ambitious programme: <br />
:» [[K-Regio MitoFit]] <br />
<br />
:'''MitoFit Training Camp 2016 Kuehtai AT:''' Immediately following EBEC 2016 organized by Paolo Bernardi in Riva del Garda, the first ‘MitoFit Training Camp’ will take place on 2016 July 7-13, at the High Altitude Training Center Kühtai at an altitude of 2020 m. The MitoFit Training Camp will provide a unique opportunity to receive first-hand introductions to state-of-the-art diagnostic monitoring of mitochondrial respiratory function. A wide range of areas will be covered on protective medicine, exercise physiology, mitochondrial pharmacology, aging, to comparative mitochondrial physiology (cell types, tissues, species).<br />
:» [[MitoFit Science Camp 2016 Kuehtai AT]] <br />
<br />
* '''014'''. 2015-09-02: '''Defining mt-hypoxia at cytochrome redox steady-states''': » [[Harrison 2015 J Appl Physiol]]<br />
: '''Open Innovation''': the O2k-Innovation is not yet in series production, but some special innovations are now available:<br />
:» [[TIP2k-Module]] with 200 µL (new) or 500 µl syringes, for steady-state hypoxia and more.<br />
:» [[O2k-Titration_Set |Oroboros racks]] make experiments with SUIT protocols more convenient.<br />
*'''013'''. 2015-07-31: To the heart of the Amazon - Phenotypic mitochondrial respiratory control differs widely from mouse to man. Does this mean that these hearts have different mitochondrial fitness? What can we learn from comparative mitochondrial physiology? Find out more: » [[MiPNet20.12 IOC103 Manaus]] <br />
:The Oroboros-team sends you best wishes for a MitoFit Summer<br />
:* from this year's KrassFit/XLETIX challenge in Kühtai (Tyrol, Austria). » [[Oroboros MitoFit team KrassFit2015 Kuehtai]]<br />
:* joining with Lemawork Ketema (Ethiopia): Wings for Life World Run champion 2014 and 2015, running 79.9 km (49.9 miles) and this year's Rio de Janeiro Marathon runner #2. »[[K-Regio MitoFit#Becoming_.E2.80.98MitoFit.E2.80.99_.E2.80.93_a_trend_towards_a_new_lifestyle.3F| Becoming MitoFit]]<br />
* ''Summer holiday break during August 2015.''<br />
* '''012'''. 2015-07-16: High fat/high fructose and modulation of hepatic mitochondrial function. » [[Aroor 2015 Diabetes]]<br />
* '''011'''. 2015-07-07: MitoFit quality above quantity in resistance training. » [[Porter 2015 Med Sci Sports Exerc]]<br />
* '''010'''. 2015-07-01: Light in the powerhouse of the cells - SUIT protocols for measurement of respiration and H<sub>2</sub>O<sub>2</sub> production: » [[Makrecka-Kuka 2015 Biomolecules]]<br />
* '''009'''. 2015-06-23: Fit and fat - the good, the bad, and the ugly. » [[Schoettl 2015 Endocrinology]]<br />
* '''008'''. 2015-06-16: HIT and fit: 2-weeks of high intensity interval training. » [[Vincent 2015 Front Physiol]]<br />
* '''007'''. 2015-06-09: Combined training and good news for the aging population. » [[Irving 2015 J Clin Endocrinol Metab]]<br />
* '''006'''. 2015-06-02: Aggression and mitochondrial fitness taken from a friend: acquisition of mtDNA from host cells. » [[Tan 2015 Cell Metab]] - This is another exciting publication not in line with the controversial 'Warburg effect'.<br />
* '''005'''. 2015-05-26: Among the fittest, females are superior - but not always welcome. » [[Soares 2015 PLoS One]]<br />
* '''004'''. 2015-05-18: Exercise as medicine: protecting from diabetic cardiomyopathy. » [[Hafstad 2015 Antioxid Redox Signal]]<br />
* '''003'''. 2015-05-13: The power of the mind: the cortex as a critical determinant of muscle strength. » [[Clark 2014 J Neurophysiol]]<br />
* '''002'''. 2015-05-08: Depressed cellular energetics correlates with depressive states of mind: high-resolution respirometry in blood cells (PBMC). » [[Karabatsiakis 2014 Translational Psychiatry]]<br />
* '''001'''. 2015-05-05: Any type of exercise helps - strength and endurance training in the fight against obesity. » [[Benito 2015 J Appl Physiol]]<br />
<br />
<br />
=== Feedback === <br />
* ''.. the mito-fit paper on the elderly was great!'' [[Hickey AJ |'''Anthony Hickey''']]; O2k-Network Lab: [[NZ Auckland Hickey AJ]] (2015-06-10).<br />
* ''Thank you for the update publication. It's quite inspiring.'' [[Ping Z| '''Ping Zheng''']] (2015-06-09).<br />
* ''Thanks for sending this interesting study conducted in PBMC for the bioblast group.'' [[Oliveira MF |'''Marcus Oliveira''']]; O2k-Network Lab: [[BR Rio de Janeiro Oliveira MF ]](2015-05-08).<br />
* ''This is the first glimpse I have seen of MitoFit, and I have to tell you it's brilliant! When we launched Team MitoCanada a few years back combined with all the recent research and correlation to general health and Mito health, it just seems like the natural progression. We are taking a new approach to mitochondrial health as well with MitoCanada and reading your webpage really confirms the need to consider the full spectrum of mitochondrial health as part of our awareness strategy.'' [[Blaine P|'''Blaine Penny''']], President and CEO of [[MitoCanada Foundation]] and winner of the [http://runningmagazine.ca/65k-wins-wings-for-life-world-run-nigara/ Wings for Life World Run in Niagara Falls 2015] (2015-05-06).<br />
<br />
<br />
<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Additional label::MitoFit news]]<br />
|?Was published in year=Year<br />
|?Has title=Reference<br />
|?Mammal and model<br />
|?Tissue and cell<br />
|?Stress <br />
|?Diseases <br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=descending<br />
}}<br />
<br />
[[Category:Mitochondrial Global Network]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Talk:O2k-Network&diff=246688Talk:O2k-Network2024-03-29T05:04:07Z<p>Gnaiger Erich: </p>
<hr />
<div>[[Image:BB-Bioblast.jpg|left|40px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]]<br />
== Popular Bioblast page ==<br />
:::[[O2k-Network]] has been accessed more than<br />
::::* 140,000 times (2019-12-11)<br />
::::* 130,000 times (2019-07-22)<br />
::::* 120,000 times (2017-06-15) <br />
::::* 110,000 times (2016-10-28) <br />
:::* '''100,000 times''' (2016-05-30) <br />
::::* 90,000 times (2016-03-12) <br />
::::* 80,000 times (2015-12-15) <br />
::::* 70,000 times (2015-08-01)<br />
::::* 60,000 times (2015-02-11)<br />
::::* 50,000 times (2014-08-28)<br />
::::* 40,000 times (2014-05-05)<br />
::::* 30,000 times (2013-11-09)<br />
::::* 20,000 times (2013-04-18)<br />
::::* 10,000 times (2012-04-04)<br />
<br />
<br />
== The term MiPNet ==<br />
=== Mitochondr Physiol Network - Oroboros on-line journal ===<br />
::::* [[Mitochondr Physiol Network]] - the Open Access Oroboros 'on-line journal' is well established for publication of the [[O2k-Manual]], [[O2k-Protocols]], [[O2k-Workshops]] and more. These publications are cited in the scientific literature, and a track record is maintained for the sequence of editions. Perhaps we will be able to give the '''Mitochondr Physiol Network''' an even more formal basis in the system of scientific publications.<br />
<br />
=== MiPNet News - Email circulars ===<br />
::::* ''MiPNet News'' originated as the ciruclar to Oroboros customers. But we actually circulate it way beyond our reference labs for various announcements, in addition to [[Bioblast alert]].<br />
<br />
=== Oroboros O2k-Network Labs (formerly: MiPNet Labs) ===<br />
::::* '''MiPNet''': Originated as the ORO network of reference labs. When the [[MiPsociety |MiP''society'']] was initiated later (MiP''conference'' 2003), there was some concern of confusion between the MiPNet and the MiP''society''.<br />
::::* '''O2k-WorldWide''' or '''O2k-WorldWide MiPNet''': This term was introduced to label consistently the main sections on the ORO website and the [[O2k-Compendium]]: O2k-Info; [[O2k-Manual]]; [[O2k-Protocols]]; [[O2k-Publications]]; [[O2k-WorldWide]]; [[O2k-Workshops]]. Later, O2k-Info was changed to [[OROBOROS:About]], putting more emphasis on the label Oroboros. The term '''[[Oroboros O2k]]''' gained importance with the introduction of the [[NextGen-O2k]] as the follow-up to the [[O2k-FluoRespirometer]].<br />
<br />
== Skin Punch Biopsy Oximetry ==<br />
<br />
:::: Dear friends,<br />
<br />
:::: I have access to patients skin punch biopsies, and I would really like to perform oximetry on them. However, I'm afraid there's not enough cells in a punch biopsy.<br />
:::: Has anyone had experience with skin biopsies (rather than the muscle biopsies)? punch biopsies?<br />
<br />
:::: Any help would be much appreciated,<br />
:::: thanks in advance,<br />
:::: Amir</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=O2k-Fluo_LED2-Module&diff=246687O2k-Fluo LED2-Module2024-03-29T05:02:28Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
__TOC__<br />
{{MitoPedia without banner<br />
|abbr=<br />
|description=[[Image:Fluorescence-Control Unit lettered.jpg|180px|right]] The '''O2k-Fluo LED2-Module''' is a component of the O2k-Fluorometer (O2k-Series D to G). It is an amperometric add-on module to the [[O2k-Core]] (O2k-Series D to G), adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex® UltraRed), ATP production (Magnesium Green™), mt-membrane potential (Safranin, TMRM, Rhodamine 123), Ca<sup>2+</sup> (Calcium Green™), and numerous other applications open for O2k-user innovation.<br />
|info=[https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ '''Product details and purchase information''']<br />
}}<br />
<br />
== Scope of application ==<br />
{{Template:Scope of fluorescence application}}<br />
<br />
== Select LED Fluo-Sensors ==<br />
{{Template:Select fluorescence sensor}}<br />
<br />
== Fluorescence dyes and settings ==<br />
{{Template:Fluorescence dyes and settings}}<br />
<br />
== Mounting a filter cap ==<br />
:::* ''See'': [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a filter cap]]<br />
<br />
== Stoppers ==<br />
{{Template:Stoppers for fluorometry}}<br />
<br />
== Connect LED Fluo-Sensor to O2k ==<br />
:::# [[Fluorescence-Control_Unit#Setup_of_the_O2k-Fluorescence_LED2-Module|Setup of the O2k-Fluo LED2-Module]]<br />
:::# [[Fluorescence-Control_Unit#Power_on|Power on]]<br />
<br />
== Electronic settings and LED intensity ==<br />
:::* ''See'': [[Fluorescence-Control_Unit#O2k-Guide|Electronic settings and LED intensity]]<br />
<br />
== O2k signal and output ==<br />
::::# [[O2k signals and output#Signal of the O2k-Core and add-on modules |O2k signal]]: The O2k-Fluo LED2-Module is operated through the amperometric (Amp)-Channel of the O2k.<br />
::::# [[O2k signals and output#O2k output |O2k output]]: type I, II, or III, or combinations.<br />
<br />
== DatLab ==<br />
<br />
[[Image:DL7graph_select_plots_amp.jpg|thumb|250px|alt=Check Amp raw signal form Graph/Select Plots to display the fluorometric signal| Graph / Select plots ]]'''Graph layout''': Three plots are available in DatLab based on the recorded signal: '''Amp Raw Signal''', '''Amp Calibrated''', and '''Amp Slope'''<nowiki>. These plots can be selected from the drop-down lines and displayed with their check boxes either on the Y1 or Y2 [Graph layout / Select Plots].</nowiki> <br />
<br />
<br />
:::: '''Amp Raw Signal''' displays the raw voltage (including amplification) as recorded by the O2k at a given gain setting. <br />
<br />
:::: '''Amp Calibrated''' is the signal after calibration with the parameters set in the O2k-MultiSensor Calibration window. <br />
<br />
:::: '''Amp slope''' is the time derivative of the '''calibrated''' signal, multiplied by '''1000''', in units [mV(conc. Unit during calibration)/s], so if the signal was calibrated in µM [nmol/ml] the unit of the slope is pmol/(s ml). To obtain the slope of the raw signal check the appropriate box in the calibration window ([[DatLab]] 5.1.0.130 and above).<br />
<br />
:::: '''Graphs''' can be generated to display oxygen and fluorescence data, or several graphs can be added to display oxygen and fluorescence data separately. Layout templates are provided, which can be modified and saved as appropriate. All graph settings can be saved as user-defined layouts, see [[MiPNet19.01C DatLab Guide]].<br />
<br />
<br />
<br />
== O2k-Fluorometry and the TIP2k ==<br />
::::* [[Titration-Injection microPump |TIP2k]]: Our tests indicated that the fluorometric signal is not affected by the TIP2k needle inserted into the 2-mL O2k-chamber.<br />
<br />
== Troubleshooting ==<br />
:::* ''-See'': [[Defective fluorescence module]]<br />
== References ==<br />
[[Image:O2k-Publications.jpg|left|116px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» O2k-Publications'''</span><br />
<div class="mw-collapsible-content"><br />
'''»[[O2k-Publications: Topics]]'''<br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
Default sorting: chronological. Empty fields appear first in ascending order. <br />
{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]]<br />
|?Was published in year<br />
|?Has title<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was published in year<br />
|order=descending<br />
}}<br />
</div><br />
</div><br />
<br /><br />
<br />
<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» O2k-Publications: O2k-Fluorometry - Abstracts'''</span><br />
<div class="mw-collapsible-content"><br />
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.<br />
{{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]] <br />
|?Was submitted in year<br />
|?Has title<br />
|?Mammal and model=Organism<br />
|?Tissue and cell=Tissue;cell<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was submitted in year<br />
|order=descending<br />
}}<br />
</div><br />
</div><br />
<br /><br />
<br />
==Additional resources==<br />
::::» [[O2k-FluoRespirometer]]<br />
::::» [[MiPNet22.11 O2k-FluoRespirometer manual]]<br />
::::» [[MiPNet17.05 O2k-Fluo LED2-Module|O2k-Fluo Manual]]<br><br />
::::» [[O2k-Demo_experiments#O2k-Demo_O2k-MultiSensor |O2k-Demo experiments]]<br><br />
::::» [[O2k-Catalogue:_O2k-Modules|O2k-Modules]]<br><br />
<br />
[[File:Questions.jpg|left|40px]]<br />
<br /><br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span><br />
<div class="mw-collapsible-content"><br />
::: '''Specific'''<br />
::::» [[O2k-Fluorometer Series G]]<br />
::::» [[Amplex UltraRed|Amplex® UltraRed]]<br />
::::» [[Calcium green|Calcium Green™]]<br />
::::» [[Magnesium green|Magnesium Green™]]<br />
::::» [[Safranin]]<br />
::::» [[TMRM]]<br />
::::» [[Fluorescence-Control Unit]] <br />
::::» [[Fluorescence-Sensor Blue]] <br />
::::» [[Fluorescence-Sensor Green]]<br />
<br />
::: '''General'''<br />
::::» [[HRR]]<br />
::::» [[mt-membrane potential]]<br />
::::» [[O2k-chamber]]<br />
::::» [[O2k-Core]] <br />
::::» [[O2k-Main Unit]]<br />
::::» [[O2k signals and output#O2k output |O2k output]]<br />
::::» [[O2k signals and output#Signal of the O2k-Core and add-on modules |O2k signal]]<br />
::::» [[Oroboros O2k]]<br />
</div><br />
</div><br />
<br /><br />
<br />
<br />
<br />
{{MitoPedia methods<br />
|mitopedia method=Fluorometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-FluoRespirometry<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Oroboros_O2k&diff=246686Oroboros O2k2024-03-29T04:59:00Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
__TOC__<br />
{{MitoPedia without banner<br />
|abbr=<br />
|description=[[File:NextGen-O2k All-in-one 2023.jpg|300px|right|NextGen-O2k all-in-one]]<br />
'''Oroboros O2k''' - the modular system for [[high-resolution respirometry]] (HRR) for mitochondria and cell research. Oroboros delivers the O2k-technology for high-resolution respirometry in mitochondria and cell research. The O2k allows the measurement of respiration at controlled oxygen levels, combined with redox biology (NADH and CoQ), ROS production, mitochondrial membrane potential, ATP production, Ca<sup>2+</sup>, or pH. HRR expands to HRPB: High-Resolution PhotoBiology. <br />
<br />
Small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells to living cells and tissues slices. <br />
<br />
The modular O2k-concept is supported by [[DatLab |DatLab]], with high flexibility for extension by add-on [https://www.oroboros.at/index.php/product-category/products/o2k-modules/ O2k-Modules]. All O2k-Modules are supported by the [[NextGen-O2k]]. The [https://www.oroboros.at/index.php/product/q-module/ O2k-Q-Module] and the [https://www.oroboros.at/index.php/product/nadh-module/ O2k-NADH-Module] are exclusively supported by the NextGen-O2k, whereas the O2k-J provides the basis for all other HRR application but cannot be upgraded to the [https://www.oroboros.at/index.php/product/nextgen-o2k-redox/ NextGen-O2k Redox]. The globally tested and trusted high-resolution O2k technology prioritizes both quality and scientific research output in the field of mitochondrial physiology and pathology, extended to PhotoBiology.<br />
|info= [https://www.oroboros.at/index.php/o2k-products/ Purchase information], [[MiPNet14.10 O2k-Top 10 |O2k-Top 10]]<br />
}}<br />
[[File:Questions.jpg|left|40px]]<br />
<br><br />
<br /><br />
<br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F7F8FF"><br />
:::» <span style="font-size:105%; color:#424242">'''The most common applications of the [[Oroboros O2k]]'''</span><br />
<div class="mw-collapsible-content"><br />
The O2k is used in basic and applied research to study the respiration of tissues, cells and mitochondria. Fairly simple to highly complex, comprehensive experimental protocols can be applied to assess either basal mitochondrial characteristics or obtain diagnostic insight into respiratory pathways. Topically, studies using the O2k range from [[O2k-Publications: Comparative MiP;environmental MiP|comparative physiological]] investigations addressing evolutionary and environmental aspects of mitochondrial metabolism over the energetics related to exercise physiology, [[O2k-Publications: Exercise physiology;nutrition;life style|nutrition and life style]], to aspects of clinical interest such as the impact of [[O2k-Publications: Mitochondrial medicine|metabolic diseases]], [[O2k-Publications: Cancer|cancer]], or [[O2k-Publications: Pharmacology;toxicology|pharmacological interventions]].<br />
<br/><br/><br/><br />
::: '''The most prominent applications of the O2k'''<br />
<br />
::::* <span style="color:#424242">'''Studies on exercise physiology;nutrition;life style'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[MiP area::Exercise physiology;nutrition;life style]] |format=count}} [[O2k-Publications: Exercise physiology;nutrition;life style|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Diagnosis of acquired and genetic mitochondrial diseases'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Inherited]] |format=count}} [[O2k-Publications: Mitochondrial disease|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Diagnosis of myopathies and neuromuscular pathologies'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Myopathy]] |format=count}} [[O2k-Publications: Myopathy|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on cell function and cell death - apoptosis and necrosis'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Cell death]] |format=count}} [[O2k-Publications: Cell death|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Measurement of oxidative stress'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Oxidative stress;RONS]] |format=count}} [[O2k-Publications: RONS; Oxidative stress|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on ischemia-reperfusion injury'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury and adaptation::Ischemia-reperfusion]] |format=count}} [[O2k-Publications: Ischemia-reperfusion|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on aging and senescence'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Aging;senescence]] |format=count}} [[O2k-Publications: Aging; senescence|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Pharmacological tests'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[MiP area::Pharmacology;toxicology]] |format=count}} [[O2k-Publications: Pharmacology;toxicology|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''Studies on Diabetes 2'''</span><br />
::::::» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Diabetes]] |format=count}} [[O2k-Publications: Diabetes 2|O2k-Publications in the MiPMap]]<br />
<br />
::::* <span style="color:#424242">'''For more applications, browse through the {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] |format=count}} [[O2k-Publications:_Topics|O2k-Publications in the MiPMap]].'''</span><br />
</div><br />
</div><br />
<br />
<br /><br />
[[File:NextGen-O2k flyer A4.jpg|right|400px]]<br />
__TOC__<br />
<br />
== Oroboros O2k: development and distribution ==<br />
<br />
[[File:Modular system scheme - new Nextgen Logo.png|300px|left]]<br />
::::* The O2k is developed by [[OROBOROS INSTRUMENTS |Oroboros Instruments]] in cooperation with [http://www.wgt.at WGT] and produced in Austria by WGT.<br />
<br />
::::* High-resolution respirometry: HRR is the basis for combined measurement of respiration with redox biology (NADH and CoQ), ROS production, mitochondrial membrane potential, ATP production, Ca<sup>2+</sup>, or pH.<br />
<br />
::::* [[TIP2k-Module|Titration-Injection microPump TIP2k]]: The O2k-TIP2k combination allows operation with programmable titration regimes, feedback control, and at steady-states, yielding a new flexibility in experimental design by combining the technical advantages of closed and open systems approaches. Automatic titrations for kinetics; feedback-controlled instrumental background tests; respiration in a closed or open system, in aerobic-anaerobic transitions or at steady-state oxygen concentrations, covering the entire physiological oxygen range and the extremes of hypoxia.<br />
<br />
::::* O2k-Modules can be combined with the O2k, and can be added any time to extend the O2k according to instrument comparability and application-specific objectives: [[Q-Module|Q]] (Q-redox state), [[NADH-Module|NAD(P)H autofluorescence]] (NADH-redox state], [[PB-Module|PhotoBiology]] (photosynthesis), [[O2k-Fluo Smart-Module| fluorometry]] (H<sub>2</sub>S, ATP, mitochondrial membrane potential, Ca<sup>2+</sup>), [[O2k-TPP+ ISE-Module|potentiometry]] (pH, TPP+ or TPMP+ for mitochondrial membrane potential, Ca<sup>2+</sup>) and [[O2k-NO Amp-Module|amperometry]] (NO, H<sub>2</sub>S).<br />
<br />
::::* Auxiliary O2k-Tools provide extended support in the context of high-resolution respirometry: [[Oxia]] the O2 regime controller for the O2k; [[Microbalance-Set|Microbalance]], [[O2k-Dissection Set]].<br />
<br />
<br />
== What is an Oxygraph and the O2k? ==<br />
[[Image:O2.jpg|left|60px|link=Oxygen|Dioxygen]]<br />
=== Oxygraph: O2 ===<br />
::::* <big>'''O2'''k:</big> The O2k measures oxygen ([[Oxygen |dioxygen, '''O<sub>2</sub>''']]) as a gas dissolved in aqueous solution. <br />
::::* With the O2k, experimental data are displayed not only of oxygen concentration over time; but real-time transformation of these data allows the experimenter to follow '''[[respiration]]''' (oxygen consumption, oxygen flux) of biological and biochemical samples while the experiment is still running.<br />
::::* An Oxygraph is not used to measure oxygen concentration in a tissue, but for high-resolution analysis of respiratory activity of biological or biochemical samples transferred into the experimental chamber. <br />
<br />
<br />
[[File:Pesta 2012 Methods Mol Biol Fig7A.jpg|250px|link=Pesta_2012_Methods_Mol_Biol|Pesta and Gnaiger 2012]]<br />
:::: [[Image:O2k-Protocols.jpg|right|230px|link=Gnaiger_2014_MitoPathways|MitoPathways]]Specific [[substrate-uncoupler-inhibitor titration]] (SUIT) protocols are applied in studies of cell respiration or mitochondrial activity. In experiments with living cells, different coupling control states can be studied to evaluate ROUTINE respiratory activity, respiration coupled to ATP production, respiratory excess capacity, residual oxygen consumption, and, by extension of conventional protocols, intactness of cell membranes. In mitochondrial preparations such as isolated mitochondria, permeabilized cells and tissue samples, or homogenized tissue, quantitative information is obtained on selected mitochondrial pathways, particularly of the process of '''[[oxidative phosphorylation]] (OXPHOS)''' in mitochondria and bacteria. Comprehensive OXPHOS analysis leads us beyond the highly informative process of oxygen consumption, when high-resolution respirometry is combined with O2k-MultiSensor modules to simultaneously measure the production of reactive oxygen species or mitochondrial membrane potential, nitric oxide production, acid-base balance or calcium with O2k-FluoRespirometry or ion selective electrodes. In the O2k, these bioenergetic parameters are always evaluated in combination with the measurement of oxygen concentration and respiration.<br />
<br />
<br />
<br />
=== Oroboros O2k: O2k and high-resolution respirometry ===<br />
<br />
::::* <big>O'''2k''':</big> The O2k is the '''2'''<sup>nd</sup> generation of our high-resolution respirometers. The first model was available as of [[OROBOROS 1992|1992]]. Development of the new O'''2k''' started in '''2k''' (2000) and the first O'''2'''k was delivered in [[OROBOROS 2002|'''2'''00'''2''']]. <br />
<br />
::::* <big>O2'''k''':</big> The O2k has two experimental chambers ('''k''': Kammern, German for chambers). [[O2k-chamber |Why 0.5 to 2 mL?]]<br />
<br />
::::* <big>'''Power-O2k''':</big> Several O2k units are operated simultaneously ('''P'''1, '''P'''2, ..) to combine high-resolution respirometry with [[Power-O2k |high-output]].<br />
<br />
<br />
:::: '''High-resolution respirometry''' (HRR) is the result of our long-term expertise in instrumental design, software development, mitochondrial physiology, clinical and pharmacological applications, and [[O2k-Network| world-wide scientific cooperation]]. The innovative O2k-developments provide a system of highest quality and distinguish HRR as a method and concept. <br />
<br />
:::: HRR is defined on the basis of instrumental features of the O2k. The limit of detection of oxygen flux is as low as 1 pmol O<sub>2</sub>.s<sup>-1</sup>.mL<sup>-1</sup>. Oxygen backdiffusion at zero oxygen is <4 pmol.s<sup>-1</sup>.mL<sup>-1</sup>. Oxygen consumption by the polarographic oxygen sensor at air saturation and standard barometric pressure (100 kPa) is 2.7 pmol.s<sup>-1</sup>.mL<sup>-1</sup> (± 0.9 SD; 114 test runs at 37 °C). These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for automatic background correction of oxygen flux (DatLab). Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Signal noise at zero oxygen concentration is <0.05 µM O<sub>2</sub>.<br />
[[File:NextGen O2k + close detail.png|right|530px]]<br />
<br />
:::: The Oroboros O2k is the unique state-of-the-art instrument for HRR - where high-resolution counts: <br />
<br />
::::* with small amounts of cells or tissue, <br />
::::* at low respiratory activities, <br />
::::* multiple substrate-uncoupler-inhibitor titrations (SUIT protocols) with resolution of changes in oxygen flux over prolonged incubation times,<br />
::::* flexibility of O2k-MultiSensor applications,<br />
::::* fast transitions at low oxygen levels,<br />
::::* stability and quality control. <br />
<br />
:::: Today, such high resolution meets rapidly increasing demands, reflected by the increasing number and high level of [[O2k-Publications]]. Applications of the O2k in leading [[O2k-Network| O2k-Network Labs]] are the driving forces for a rapid advancement of mitochondrial physiology. Open access to the [[O2k-Manual]] is an integral part of the concept of [[OROBOROS INSTRUMENTS |Oroboros Instruments]] on [[Gentle Science]] and Scientific Social Responsibility. With sharing of [[O2k-Procedures]] and implementation of quality control, the study of mitochondrial and cell respiration emerges as a quantitative science. Thus high-resolution respirometry provides the foundation for comparative mitochondrial physiology, biomedical research, translation into clinical applications, establishing quantitative functional mitochondrial diagnosis. <br />
<br />
::::* Sole source information: [http://www.bioblast.at/images/6/60/Oroboros_SoleSource.pdf '''pdf''']<br />
::::* [[Oroboros FAQ|Frequently Asked Questions]] <br />
<br />
:::: The Oroboros O2k sets a world-wide standard in high-resolution respirometry - for mitochondrial studies in cell physiology and pathology. <br />
<br />
:::: In contrast to merely offering an oxygen measuring device, the Oroboros O2k is integrated into a systems approach to high-resolution respirometry. This spans from experimental tools which are invaluable in the lab (e.g. [[ISS]]), or little aids that are crucial for tissue preparation (forceps), mitochondrial respiration medium ([[MiR05-Kit]]), [[O2k-Procedures| substrate-uncoupler-inhibitor titration protocols]], and the unique [[O2k-Workshops]].<br />
<br />
<br />
<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Talk:High-resolution_respirometry&diff=246685Talk:High-resolution respirometry2024-03-28T18:12:55Z<p>Gnaiger Erich: </p>
<hr />
<div>[[Image:BB-Bioblast.jpg|left|30px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]]<br />
<br />
[[File:O2k-Core to FluoRespirometer.jpg|right|400px |O2k-Core to FluoRespirometer]]<br />
<br />
== Popular Bioblast page ==<br />
::: [[High-resolution respirometry]] has been accessed more than <br />
::::* 40,000 times (2019-07-22)<br />
::::* 35,000 times (2019-03-13)<br />
::::* 30,000 times (2017-04-05)<br />
::::* 25,000 times (2016-09-18)<br />
::::* 20,000 times (2016-02-27)<br />
::::* 15,000 times (2015-08-11)<br />
::::* 10,000 times (2014-11-22)<br />
::::* 5,000 times (2013-08-25)</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=High-resolution_respirometry&diff=246684High-resolution respirometry2024-03-28T18:12:20Z<p>Gnaiger Erich: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=HRR<br />
|description=[[Image:O2k-Fluorometer.jpg|200px|left|O2k-FluoRespirometer]] '''High-resolution respirometry, HRR''', is the state-of-the-art approach in mitochondria and cell research to measure respiration in various types of [[mitochondrial preparations]] and [[living cells]] combined with MultiSensor modules. <br />
<br />
Mitochondrial function and dysfunction have gained increasing interest, reflecting growing awareness of the fact that mitochondria play a pivotal role in human health and disease. HRR combines instrumental accuracy and reliability with the versatility of applicable protocols, allowing practically unlimited addition and combination of substrates, inhibitors, and uncouplers in the [[Oroboros O2k]]. Substrate-uncoupler-inhibitor titration (SUIT) protocols allow the interrogation of numerous mitochondrial pathway and coupling states in a single respirometric assay. Mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory coupling and pathway control patterns. <br />
<br />
The O2k is a sole source technology, with no other available instrument meeting its specifications for high-resolution respirometry. Technologically, HRR is based on the Oroboros O2k, combining optimized chamber design, application of oxygen-tight materials, electrochemical sensors, Peltier-temperature control, and specially developed software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with both, a closed-chamber or open-chamber mode of operation ([[TIP2k-Module|TIP2k]]). Standardized calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background oxygen flux (systemic flux compensation) provide the experimental basis for high accuracy of quantitative results and quality control in HRR. HRR can be extended for MultiSensor analysis by using the [[O2k-Fluo Smart-Module]]. [[Smart Fluo-Sensor]]s are integrated into the O2k to measure simultaneously fluorometric signals using specific fluorophores. Potentiometric modules are available with ion-selective electrodes (pH, TPP<sup>+)</sup>. The [[PB-Module]] extends HRR to PhotoBiology with accurate control of the light intensity and measurement of photosynthesis. The O2k-J and the NextGen-O2k support all these O2k-Modules. The [[NextGen-O2k]] all-in-one, however, is unique in supporting Q-Redox and NADH-Redox Modules.<br />
|info=[[Oroboros O2k]]<br />
}}<br />
<br />
::::* <big>'''Power O2k''':</big> Several O2k units are operated simultaneously ('''P'''1, '''P'''2, ..) to combine high-resolution respirometry with [[Power O2k-Respirometer |high-output]].<br />
<br />
<br />
== MitoPedia: O2k and high-resolution respirometry ==<br />
::::» [[MitoPedia: O2k hardware]]<br />
::::» [[MitoPedia: DatLab]]<br />
::::» [[MitoPedia: SUIT]]<br />
<br />
<br />
== Links ==<br />
::::'''»''' [[MitoPedia: Respiratory states]] [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ET capacity]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]<br />
::::» [[Respirometry]]<br />
::::» [[MitoPedia: Respirometry]]<br />
::::» [http://en.wikipedia.org/wiki/Respirometry Wikipedia: Respirometry]<br />
::::» [[O2k Applications]]<br />
<br />
<br /><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
:::[[File:Questions.jpg|left|24px |Explanation of terms]] '''» Keywords'''<br />
<div class="mw-collapsible-content"><br />
::: '''Instrument'''<br />
::::» [[DatLab]]; » [[MitoPedia: DatLab]]<br />
::::» Instrumental background oxygen flux »» [[MiPNet14.06 Instrumental O2 background]]<br />
::::» [[Oroboros O2k-Core (O2k-Series D - G)]]<br />
::::» [[O2k-FluoRespirometer]]<br />
::::» [[O2k-MultiSensor]]<br />
::::» [[Oroboros O2k]]; » [[MitoPedia: O2k hardware]]<br />
::::» [[Power O2k-Respirometer]]<br />
::::» [[MiPNet06.03 POS-calibration-SOP |Static sensor calibration]]<br />
::::» [[TIP2k-Module|TIP2k]]<br />
<br />
::: '''Concept'''<br />
::::» [[Concentration]]<br />
::::» [[Coupling-control state]] <br />
::::» [[High-resolution respirometry]]<br />
::::» [[Mitochondrial preparations]]<br />
::::» [[Oxidative phosphorylation]]<br />
::::» [[Oxygen |Oxygen, dioxygen, O<sub>2</sub>]]<br />
::::» [[Oxygen flux]]<br />
::::» [[Electron-transfer-pathway state]]<br />
::::» [[Respiration]]<br />
::::» [[Respirometry]]; » [[MitoPedia: Respirometry]]<br />
::::» [[SUIT| Substrate-uncoupler-inhibitor titration (SUIT) protocols]]; » [[MitoPedia: SUIT]]<br />
</div><br />
</div><br />
<br /><br />
<br />
[[Image:O2k-Publications.jpg|left|116px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]<br />
<br /><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» References'''</span><br />
<div class="mw-collapsible-content"><br />
::: '''O2k-Publications'''<br />
::::* [[O2k-Publications |O2k-Publications: Chronological]]<br />
::::* [[O2k-Publications: Topics]]<br />
<br />
<br />
== Keywords: Oxygen signal ==<br />
{{Template:Keywords: Oxygen signal}}<br />
<br />
<br />
::: '''Selected references'''<br />
::::* [[Gnaiger 1995 J Bioenerg Biomembr]]<br />
::::* [[Gnaiger 2001 Respir Physiol]]<br />
::::* [[Gnaiger 2008 POS]]<br />
::::* [[Gnaiger 2020 BEC MitoPathways]]<br />
</div><br />
</div><br />
<br /><br />
<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry, Fluorometry, Spectrophotometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Respirometry, O2k-FluoRespirometry<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=High-resolution_respirometry&diff=246672High-resolution respirometry2024-03-28T09:02:51Z<p>Gnaiger Erich: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=HRR<br />
|description=[[Image:O2k-Fluorometer.jpg|200px|left|O2k-FluoRespirometer]] '''High-resolution respirometry, HRR''', is the state-of-the-art approach in mitochondria and cell research to measure respiration in various types of [[mitochondrial preparations]] and [[living cells]] combined with MultiSensor modules. Mitochondrial function and dysfunction have gained increasing interest, reflecting growing awareness of the fact that mitochondria play a pivotal role in human health and disease. HRR combines instrumental accuracy and reliability with the versatility of applicable protocols, allowing practically unlimited addition and combination of substrates, inhibitors, and uncouplers in the [[Oroboros O2k]]. Substrate-uncoupler-inhibitor titration (SUIT) protocols allow the interrogation of numerous mitochondrial pathway and coupling states in a single respirometric assay. Mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory coupling and pathway control patterns. <br />
<br />
The O2k is a sole source technology, with no other available instrument meeting its specifications for high-resolution respirometry. Technologically, HRR is based on the Oroboros O2k, combining optimized chamber design, application of oxygen-tight materials, electrochemical sensors, Peltier-temperature control, and specially developed software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with both, a closed-chamber or open-chamber mode of operation ([[TIP2k-Module|TIP2k]]). Standardized calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background oxygen flux (systemic flux compensation) provide the experimental basis for high accuracy of quantitative results and quality control in HRR. HRR can be extended for MultiSensor analysis by using the [[O2k-Fluo Smart-Module]]. [[Smart Fluo-Sensor]]s are integrated into the O2k to measure simultaneously fluorometric signals using specific fluorophores. Potentiometric modules are available with ion-selective electrodes (pH, TPP<sup>+)</sup>. The [[PB-Module]] extends HRR to PhotoBiology with accurate control of the light intensity and measurement of photosynthesis. The O2k-J and the NextGen-O2k support all these O2k-Modules. The [[NextGen-O2k]] all-in-one, however, is unique in supporting Q-Redox and NADH-Redox Modules.<br />
|info=[[Oroboros O2k]]<br />
}}<br />
[[File:O2k-Core to FluoRespirometer.jpg|right|400px |O2k-Core to FluoRespirometer]]<br />
<br />
<br />
::::* <big>'''Power O2k-Respirometer''':</big> Several O2k units are operated simultaneously ('''P'''1, '''P'''2, ..) to combine high-resolution respirometry with [[Power O2k-Respirometer |high-output]].<br />
<br />
<br />
== MitoPedia: O2k and high-resolution respirometry ==<br />
::::» [[MitoPedia: O2k hardware]]<br />
::::» [[MitoPedia: DatLab]]<br />
::::» [[MitoPedia: SUIT]]<br />
<br />
<br />
== Links ==<br />
::::'''»''' [[MitoPedia: Respiratory states]] [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ET capacity]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]<br />
::::» [[Respirometry]]<br />
::::» [[MitoPedia: Respirometry]]<br />
::::» [http://en.wikipedia.org/wiki/Respirometry Wikipedia: Respirometry]<br />
::::» [[O2k Applications]]<br />
<br />
<br /><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
:::[[File:Questions.jpg|left|24px |Explanation of terms]] '''» Keywords'''<br />
<div class="mw-collapsible-content"><br />
::: '''Instrument'''<br />
::::» [[DatLab]]; » [[MitoPedia: DatLab]]<br />
::::» Instrumental background oxygen flux »» [[MiPNet14.06 Instrumental O2 background]]<br />
::::» [[Oroboros O2k-Core (O2k-Series D - G)]]<br />
::::» [[O2k-FluoRespirometer]]<br />
::::» [[O2k-MultiSensor]]<br />
::::» [[Oroboros O2k]]; » [[MitoPedia: O2k hardware]]<br />
::::» [[Power O2k-Respirometer]]<br />
::::» [[MiPNet06.03 POS-calibration-SOP |Static sensor calibration]]<br />
::::» [[TIP2k-Module|TIP2k]]<br />
<br />
::: '''Concept'''<br />
::::» [[Concentration]]<br />
::::» [[Coupling-control state]] <br />
::::» [[High-resolution respirometry]]<br />
::::» [[Mitochondrial preparations]]<br />
::::» [[Oxidative phosphorylation]]<br />
::::» [[Oxygen |Oxygen, dioxygen, O<sub>2</sub>]]<br />
::::» [[Oxygen flux]]<br />
::::» [[Electron-transfer-pathway state]]<br />
::::» [[Respiration]]<br />
::::» [[Respirometry]]; » [[MitoPedia: Respirometry]]<br />
::::» [[SUIT| Substrate-uncoupler-inhibitor titration (SUIT) protocols]]; » [[MitoPedia: SUIT]]<br />
</div><br />
</div><br />
<br /><br />
<br />
[[Image:O2k-Publications.jpg|left|116px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]<br />
<br /><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
::: <span style="font-size:105%; color:##424242">'''» References'''</span><br />
<div class="mw-collapsible-content"><br />
::: '''O2k-Publications'''<br />
::::* [[O2k-Publications |O2k-Publications: Chronological]]<br />
::::* [[O2k-Publications: Topics]]<br />
<br />
<br />
== Keywords: Oxygen signal ==<br />
{{Template:Keywords: Oxygen signal}}<br />
<br />
<br />
::: '''Selected references'''<br />
::::* [[Gnaiger 1995 J Bioenerg Biomembr]]<br />
::::* [[Gnaiger 2001 Respir Physiol]]<br />
::::* [[Gnaiger 2008 POS]]<br />
::::* [[Gnaiger 2020 BEC MitoPathways]]<br />
</div><br />
</div><br />
<br /><br />
<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry, Fluorometry, Spectrophotometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Respirometry, O2k-FluoRespirometry<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Template:Next_oroboros_ecosystem_agenda&diff=246663Template:Next oroboros ecosystem agenda2024-03-27T08:40:12Z<p>Gnaiger Erich: </p>
<hr />
<div><br />
2024 Week 15<br />
<br />
* '''Apr-08 Mo'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]<br />
|''' Is fatty acid oxidation (FAO) considered as CI- or CII-driven respiration? '''<br><br />
Neither. There is simultaneous electron transfer from FAO into the Q-junction through the electron transferring flavoprotein dehydrogenase complex (CETFDH) and Complex I. <br><br />
- »'''[[Fatty acid oxidation]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''«, »'''[[Gnaiger 2024 J Biol Chem]]'''« <br><br />
- communicated by »[[Timon-Gomez Alba]]« and »[[Willis Jaime R]]« <br />
|}<br />
----<br />
* '''Apr-09 Tu''' <br />
:::: 10:00 '''[[Gnaiger Erich|Erich Gnaiger]] presenting at [[MOSBRI Consensus Meeting 2024 Rome IT]]''' - ''Quality-controlled high-resolution respirometry: bacterial and clinical applications''<br />
----<br />
* '''Apr-10 We''' <br />
----<br />
* '''Apr-11 Th''' <br />
World Parkinson's Disease Day<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''"The markers of mitochondrial biogenesis were consistently affected in PD [Parkinson’s disease] patients group."''' <br><br />
* Kamienieva I, Charzyńska A, Duszyński J, Malińska D, Szczepanowska J (2023) In search for mitochondrial biomarkers of Parkinson's disease: Findings in parkin-mutant human fibroblasts. [[Kamienieva 2023 Biochim Biophys Acta Mol Basis Dis|»Bioblast link«]]<br />
:from the O2k-Network »[[PL Warsaw Szewczyk A]]«<br />
|}<br />
----<br />
* '''Apr-12 Fr'''<br />
<br />
2024 Week 16<br />
<br />
* '''Apr-15 Mo''' <br />
----<br />
* '''Apr-16 Tu''' <br />
----<br />
* '''Apr-17 We''' <br />
----<br />
* '''Apr-18 Th''' <br />
----<br />
* '''Apr-19 Fr'''<br />
<br />
2024 Week 17<br />
<br />
* '''Apr-22 Mo'''<br />
----<br />
* '''Apr-23 Tu''' <br />
----<br />
* '''Apr-24 We''' <br />
----<br />
* '''Apr-25 Th''' <br />
----<br />
* '''Apr-26 Fr'''<br />
<br />
2024 Week 18<br />
<br />
* '''Apr-29 Mo'''<br />
----<br />
* '''Apr-30 Tu''' <br />
----<br />
* '''May-01 We''' <br />
----<br />
* '''May-02 Th''' <br />
----<br />
* '''May-03 Fr'''<br />
<br />
2024 Week 19<br />
<br />
* '''May-06 Mo'''<br />
----<br />
* '''May-07 Tu''' <br />
----<br />
* '''May-08 We''' <br />
----<br />
* '''May-09 Th''' <br />
----<br />
* '''May-10 Fr'''<br />
<br />
2024 Week 20<br />
<br />
* '''May-13 Mo'''<br />
----<br />
* '''May-14 Tu''' <br />
----<br />
* '''May-15 We''' <br />
----<br />
* '''May-16 Th''' <br />
----<br />
* '''May-17 Fr'''<br />
<br />
2024 Week 21<br />
<br />
* '''May-20 Mo''' <br />
----<br />
* ''May-21 Tu''' <br />
----<br />
* '''May-22 We''' <br />
----<br />
* '''May-23 Th''' <br />
----<br />
* '''May-24 Fr'''<br />
<br />
2024 Week 22<br />
<br />
* '''May-27 Mo'''<br />
----<br />
* '''May-28 Tu''' <br />
----<br />
* '''May-29 We''' <br />
----<br />
* '''May-30 Th''' <br />
----<br />
* '''May-31 Fr'''<br />
<br />
2024 Week 23<br />
<br />
* '''Jun-03 Mo'''<br />
----<br />
* '''Jun-04 Tu''' <br />
----<br />
* '''Jun-05 We''' <br />
----<br />
* '''Jun-06 Th''' <br />
----<br />
* '''Jun-07 Fr'''<br />
<br />
2024 Week 24<br />
<br />
* '''Jun-10 Mo'''<br />
----<br />
* '''Jun-11 Tu''' <br />
----<br />
* '''Jun-12 We''' <br />
----<br />
* '''Jun-13 Th''' <br />
----<br />
* '''Jun-14 Fr'''<br />
<br />
2024 Week 25<br />
<br />
* '''Jun-17 Mo'''<br />
:::: [[Image:O2k-Workshops.png|60px|link=MiPNet28.02 IOC162 Schroecken AT]] <big> '''[[MiPNet28.11 IOC163 Schroecken AT]]'''</big> Jun 17-22<br />
<br />
----<br />
* '''Jun-18 Tu''' <br />
:::: [[Image:O2k-Workshops.png|60px|link=MiPNet28.02 IOC162 Schroecken AT]] <big> '''[[MiPNet28.11 IOC163 Schroecken AT]]'''</big> Jun 17-22<br />
----<br />
* '''Jun-19 We''' <br />
:::: [[Image:O2k-Workshops.png|60px|link=MiPNet28.02 IOC162 Schroecken AT]] <big> '''[[MiPNet28.11 IOC163 Schroecken AT]]'''</big> Jun 17-22<br />
----<br />
* '''Jun-20 Th''' <br />
:::: [[Image:O2k-Workshops.png|60px|link=MiPNet28.02 IOC162 Schroecken AT]] <big> '''[[MiPNet28.11 IOC163 Schroecken AT]]'''</big> Jun 17-22<br />
----<br />
* '''Jun-21 Fr'''<br />
:::: [[Image:O2k-Workshops.png|60px|link=MiPNet28.02 IOC162 Schroecken AT]] <big> '''[[MiPNet28.11 IOC163 Schroecken AT]]'''</big> Jun 17-22<br />
<br />
2024 Week 26<br />
<br />
* '''Jun-24 Mo'''<br />
----<br />
* '''Jun-25 Tu''' <br />
----<br />
* '''Jun-26 We''' <br />
----<br />
* '''Jun-27 Th''' <br />
----<br />
* '''Jun-28 Fr'''<br />
<br />
<br />
2024 Week 27<br />
<br />
* '''Jul-01 Mo'''<br />
----<br />
* '''Jul-02 Tu''' <br />
----<br />
* '''Jul-03 We''' <br />
----<br />
* '''Jul-04 Th''' <br />
----<br />
* '''Jul-05 Fr'''<br />
<br />
2024 Week 28<br />
<br />
* '''Jul-08 Mo'''<br />
----<br />
* '''Jul-09 Tu''' <br />
----<br />
* '''Jul-10 We''' <br />
----<br />
* '''Jul-11 Th''' <br />
----<br />
* '''Jul-12 Fr'''<br />
<br />
2024 Week 29<br />
<br />
* '''Jul-15 Mo'''<br />
----<br />
* '''Jul-16 Tu''' <br />
----<br />
* '''Jul-17 We''' <br />
----<br />
* '''Jul-18 Th''' <br />
----<br />
* '''Jul-19 Fr'''<br />
<br />
2024 Week 30<br />
<br />
* '''Jul-22 Mo'''<br />
----<br />
* '''Jul-23 Tu''' <br />
----<br />
* '''Jul-24 We''' <br />
----<br />
* '''Jul-25 Th''' <br />
----<br />
* '''Jul-26 Fr'''<br />
<br />
2024 Week 31<br />
<br />
* '''Jul-29 Mo'''<br />
----<br />
* '''Jul-30 Tu''' <br />
----<br />
* '''Jul-31 We''' <br />
----<br />
* '''Aug-01 Th''' <br />
----<br />
* '''Aug-02 Fr'''<br />
<br />
2024 Week 32<br />
<br />
* '''Aug-05 Mo'''<br />
----<br />
* '''Aug-06 Tu''' <br />
----<br />
* '''Aug-07 We''' <br />
----<br />
* '''Aug-08 Th''' <br />
----<br />
* '''Aug-09 Fr'''<br />
<br />
2024 Week 33<br />
<br />
* '''Aug-12 Mo'''<br />
----<br />
* '''Aug-13 Tu''' <br />
----<br />
* '''Aug-14 We''' <br />
----<br />
* '''Aug-15 Th''' <br />
----<br />
* '''Aug-16 Fr'''<br />
<br />
2024 Week 34<br />
<br />
* '''Aug-19 Mo'''<br />
----<br />
* '''Aug-20 Tu''' <br />
----<br />
* '''Aug-21 We''' <br />
----<br />
* '''Aug-22 Th''' <br />
----<br />
* '''Aug-23 Fr'''<br />
<br />
2024 Week 35<br />
<br />
* '''Aug-26 Mo'''<br />
----<br />
* '''Aug-27 Tu''' <br />
----<br />
* '''Aug-28 We''' <br />
----<br />
* '''Aug-29 Th''' <br />
----<br />
* '''Aug-30 Fr'''<br />
<br />
2024 Week 36<br />
<br />
* '''Sep-02 Mo'''<br />
----<br />
* '''Sep-03 Tu''' <br />
----<br />
* '''Sep-04 We''' <br />
----<br />
* '''Sep-05 Th''' <br />
----<br />
* '''Sep-06 Fr'''<br />
<br />
2024 Week 37<br />
<br />
* '''Sep-09 Mo'''<br />
----<br />
* '''Sep-10 Tu''' <br />
----<br />
* '''Sep-11 We''' <br />
----<br />
* '''Sep-12 Th''' <br />
----<br />
* '''Sep-13 Fr'''<br />
<br />
2024 Week 38<br />
<br />
* '''Sep-16 Mo'''<br />
----<br />
* '''Sep-17 Tu''' <br />
----<br />
* '''Sep-18 We''' <br />
----<br />
* '''Sep-19 Th''' <br />
----<br />
* '''Sep-20 Fr'''<br />
<br />
2024 Week 39<br />
<br />
* '''Sep-22 Su'''<br />
::::: <big> '''[[EMC2024 Ljubljana SI]]'''</big> 2024 Sep 22-26 <br />
* '''Sep-23 Mo'''<br />
::::: <big> '''[[EMC2024 Ljubljana SI]]'''</big> 2024 Sep 22-26 <br />
----<br />
* '''Sep-24 Tu''' <br />
::::: <big> '''[[EMC2024 Ljubljana SI]]'''</big> 2024 Sep 22-26 <br />
----<br />
* '''Sep-25 We''' <br />
::::: <big> '''[[EMC2024 Ljubljana SI]]'''</big> 2024 Sep 22-26 <br />
----<br />
* '''Sep-26 Th''' <br />
::::: <big> '''[[EMC2024 Ljubljana SI]]'''</big> 2024 Sep 22-26 <br />
----<br />
* '''Sep-27 Fr'''<br />
<br />
<br />
2024 Week 40<br />
<br />
* '''Sep-30 Mo'''<br />
:::: [[Image:O2k-Workshops.png|60px|link=MiPNet28.02 IOC164 Schroecken AT]] <big> '''[[MiPNet28.12 IOC164 Schroecken AT]]'''</big> Sep 30-Okt 05<br />
----<br />
* '''Okt-01 Tu''' <br />
:::: [[Image:O2k-Workshops.png|60px|link=MiPNet28.02 IOC164 Schroecken AT]] <big> '''[[MiPNet28.12 IOC164 Schroecken AT]]'''</big> Sep 30-Okt 05<br />
----<br />
* '''Okt-02 We''' <br />
:::: [[Image:O2k-Workshops.png|60px|link=MiPNet28.02 IOC164 Schroecken AT]] <big> '''[[MiPNet28.12 IOC164 Schroecken AT]]'''</big> Sep 30-Okt 05<br />
----<br />
* '''Okt-03 Th''' <br />
:::: [[Image:O2k-Workshops.png|60px|link=MiPNet28.02 IOC164 Schroecken AT]] <big> '''[[MiPNet28.12 IOC164 Schroecken AT]]'''</big> Sep 30-Okt 05<br />
----<br />
* '''Okt-04 Fr'''<br />
:::: [[Image:O2k-Workshops.png|60px|link=MiPNet28.02 IOC164 Schroecken AT]] <big> '''[[MiPNet28.12 IOC164 Schroecken AT]]'''</big> Sep 30-Okt 05<br />
<br />
2024 Week 41<br />
<br />
* '''Okt-07 Mo'''<br />
----<br />
* '''Okt-08 Tu''' <br />
----<br />
* '''Okt-09 We''' <br />
----<br />
* '''Okt-10 Th''' <br />
----<br />
* '''Okt-11 Fr'''<br />
<br />
2024 Week 42<br />
<br />
* '''Okt-14 Mo'''<br />
----<br />
* '''Okt-15 Tu''' <br />
----<br />
* '''Okt-16 We''' <br />
----<br />
* '''Okt-17 Th''' <br />
----<br />
* '''Okt-18 Fr'''<br />
<br />
2024 Week 43<br />
<br />
* '''Okt-21 Mo'''<br />
----<br />
* '''Okt-22 Tu''' <br />
----<br />
* '''Okt-23 We''' <br />
----<br />
* '''Okt-24 Th''' <br />
----<br />
* '''Okt-25 Fr'''<br />
::::: <big> '''[[14th Mitochondrial Disease Conference 2024 Padova IT]]'''</big> 2024 Oct 25-27<br />
<br />
2024 Week 44<br />
<br />
* '''Okt-28 Mo'''<br />
----<br />
* '''Okt-29 Tu''' <br />
----<br />
* '''Okt-30 We''' <br />
----<br />
* '''Okt-31 Th''' <br />
----<br />
* '''Nov-01 Fr''' <br />
<br />
2024 Week 45<br />
<br />
* '''Nov-04 Mo'''<br />
----<br />
* '''Nov-05 Tu''' <br />
----<br />
* '''Nov-06 We''' <br />
----<br />
* '''Nov-07 Th''' <br />
----<br />
* '''Nov-08 Fr'''<br />
<br />
2024 Week 46<br />
<br />
* '''Nov-11 Mo'''<br />
----<br />
* '''Nov-12 Tu''' <br />
----<br />
* '''Nov-13 We''' <br />
----<br />
* '''Nov-14 Th''' <br />
----<br />
* '''Nov-15 Fr'''<br />
<br />
<br />
2024 Week 47<br />
<br />
* '''Nov-18 Mo'''<br />
----<br />
* '''Nov-19 Tu''' <br />
----<br />
* '''Nov-20 We''' <br />
----<br />
* '''Nov-21 Th''' <br />
----<br />
* '''Nov-22 Fr'''<br />
<br />
2024 Week 48<br />
<br />
* '''Nov-25 Mo'''<br />
----<br />
* '''Nov-26 Tu''' <br />
----<br />
* '''Nov-27 We''' <br />
----<br />
* '''Nov-28 Th''' <br />
----<br />
* '''Nov-29 Fr'''<br />
<br />
2024 Week 49<br />
<br />
* '''Dez-02 Mo'''<br />
----<br />
* '''Dez-03 Tu''' <br />
----<br />
* '''Dez-04 We''' <br />
----<br />
* '''Dez-05 Th''' <br />
----<br />
* '''Dez-06 Fr'''<br />
<br />
2024 Week 50<br />
<br />
* '''Dez-09 Mo'''<br />
----<br />
* '''Dez-10 Tu''' <br />
----<br />
* '''Dez-11 We''' <br />
----<br />
* '''Dez-12 Th''' <br />
----<br />
* '''Dez-13 Fr'''<br />
<br />
2024 Week 51<br />
<br />
* '''Dez-16 Mo'''<br />
----<br />
* '''Dez-17 Tu''' <br />
----<br />
* '''Dez-18 We''' <br />
----<br />
* '''Dez-19 Th''' <br />
----<br />
* '''Dez-20 Fr'''<br />
<br />
2024 Week 52<br />
<br />
* '''Dez-23 Mo'''<br />
----<br />
* '''Dez-24 Tu''' <br />
----<br />
* '''Dez-25 We''' <br />
----<br />
* '''Dez-26 Th''' <br />
----<br />
* '''Dez-27 Fr'''</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Steininger_2002_J_Antimicrob_Chemother&diff=246661Steininger 2002 J Antimicrob Chemother2024-03-27T07:45:33Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Publication<br />
|title=Steininger C, Allerberger F, Gnaiger E (2002) Clinical significance of inhibition kinetics in ''Streptococcus pyogenes'' in response to penicillin. J Antimicrob Chemother 50:517-23. https://doi.org/10.1093/jac/dkf174<br />
|info=[http://www.ncbi.nlm.nih.gov/pubmed/12356796 PMID: 12356796 Open Access]<br />
|authors=Steininger C, Allerberger F, Gnaiger Erich<br />
|year=2002<br />
|journal=J Antimicrob Chemother<br />
|abstract=Objectives: The antibiotic mode of action against clinical isolates of ''Streptococcus pyogenes'' and physiological factors involved in modifying the inhibitory response to the antibiotic were investigated.<br />
Methods: We developed high-resolution respirometry for continuous monitoring of bacterial growth and inhibition kinetics. One hundred and ten clinical isolates from 90 paediatric patients were tested, including 48 isolates obtained from 28 patients with eradication failure. Respirometric inhibition curves were monitored at 4 mg/L penicillin G over a short 30 min period, corresponding to the drug’s serum half-life.<br />
<br />
Results: None of the clinical isolates exhibited penicillin tolerance. Latency in the respirometric response of ''S. pyogenes'' to penicillin increased significantly with decreasing strain-specific respirometric growth rate. No difference in inhibition kinetics was found in vitro for isolates from patients with or without bacteriological treatment failure.<br />
Conclusions: In streptococcal pharyngotonsillitis, tolerance is not a relevant concept to explain bacteriological treatment failure. Definitions of tolerance should be reconsidered in the framework of growth-dependent antibiotic susceptibility.<br />
|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros <br />
}}<br />
== Cited by ==<br />
::* 2 articles in PubMed (2021-12-27) https://pubmed.ncbi.nlm.nih.gov/12356796/<br />
<br />
{{Labeling<br />
|area=Respiration, mt-Biogenesis;mt-density, mt-Medicine, Pharmacology;toxicology<br />
|diseases=Infectious<br />
|organism=Eubacteria<br />
|preparations=Intact cells<br />
|couplingstates=ROUTINE<br />
|instruments=Oxygraph-2k<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Donnelly_2023_MitoFit&diff=246660Donnelly 2023 MitoFit2024-03-27T07:13:56Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Publication<br />
|title=Donnelly C, Komlódi T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-0002 — ''2024-11-17 published in [[Donnelly 2024 Redox Biol |'''Redox Biol''']].''<br />
|info=MitoFit Preprints 2023.2. [[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/b/b4/Donnelly_2023_MitoFit.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/b/b4/Donnelly_2023_MitoFit.pdf Functional hypoxia reduces mitochondrial calcium uptake.]<br/><br />
|authors=MitoFit Preprints<br />
|year=2023<br />
|journal=MitoFit Prep<br />
|abstract=Mitochondrial respiration extends beyond ATP generation, with the organelle participating in many cellular and physiological processes. Parallel changes in components of the mitochondrial electron transfer system with respiration render it an appropriate hub for coordinating cellular adaption to changes in oxygen levels. How changes in respiration under functional hypoxia (i.e., when intracellular O<sub>2</sub> levels limit mitochondrial respiration) are relayed by the electron transfer system to impact mitochondrial adaption and remodeling after hypoxic exposure remains poorly defined. This is largely due to challenges integrating findings under controlled and defined O<sub>2</sub> levels in studies connecting functions of isolated mitochondria to humans during physical exercise. Here we present experiments under conditions of hypoxia in isolated mitochondria, myotubes and exercising humans. Performing steady-state respirometry with isolated mitochondria we found that oxygen limitation of respiration reduced electron flow and oxidative phosphorylation, lowered the mitochondrial membrane potential difference, and decreased mitochondrial calcium influx. Similarly, in myotubes under functional hypoxia mitochondrial calcium uptake decreased in response to sarcoplasmic reticulum calcium release for contraction. In both myotubes and human skeletal muscle this blunted mitochondrial adaptive responses and remodeling upon contractions. Our results suggest that by regulating calcium uptake the mitochondrial electron transfer system is a hub for coordinating cellular adaption under functional hypoxia.<br><br />
|keywords=respirometry; membrane potential; skeletal muscle; exercise; coenzyme Q<br />
|editor=[[Tindle-Solomon L]]<br />
|mipnetlab=AT Innsbruck Oroboros, CH Lausanne Place N, HU Budapest Tretter L<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-5392-7876]] Donnelly Chris, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-9876-1411]] Komlodi Timea, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-7068-6165]] Cecatto Cristiane, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-6392-9229]] Cardoso Luiza HD, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-9776-7501]] Kayser Bengt, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich,[[File:ORCID.png|20px|link=https://orcid.org/0000-0001-9044-0621]] Place Nicolas<br />
{{Labeling<br />
|area=Respiration, Exercise physiology;nutrition;life style<br />
|injuries=Hypoxia<br />
|organism=Human, Mouse<br />
|tissues=Heart, Skeletal muscle, Nervous system, Other cell lines<br />
|preparations=Isolated mitochondria<br />
|topics=Calcium, mt-Membrane potential, Redox state<br />
|instruments=Oxygraph-2k, TIP2k, O2k-Fluorometer, Ca, NextGen-O2k<br />
|additional=Steady state<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Donnelly_2023_MitoFit&diff=246659Donnelly 2023 MitoFit2024-03-27T07:13:16Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Publication<br />
|title=Donnelly C, Komlódi T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-0002 — ''2024-11-17 published in [[Donnelly 2024 Redox Biol |'''Redox Biol'''].''<br />
|info=MitoFit Preprints 2023.2. [[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/b/b4/Donnelly_2023_MitoFit.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/b/b4/Donnelly_2023_MitoFit.pdf Functional hypoxia reduces mitochondrial calcium uptake.]<br/><br />
|authors=MitoFit Preprints<br />
|year=2023<br />
|journal=MitoFit Prep<br />
|abstract=Mitochondrial respiration extends beyond ATP generation, with the organelle participating in many cellular and physiological processes. Parallel changes in components of the mitochondrial electron transfer system with respiration render it an appropriate hub for coordinating cellular adaption to changes in oxygen levels. How changes in respiration under functional hypoxia (i.e., when intracellular O<sub>2</sub> levels limit mitochondrial respiration) are relayed by the electron transfer system to impact mitochondrial adaption and remodeling after hypoxic exposure remains poorly defined. This is largely due to challenges integrating findings under controlled and defined O<sub>2</sub> levels in studies connecting functions of isolated mitochondria to humans during physical exercise. Here we present experiments under conditions of hypoxia in isolated mitochondria, myotubes and exercising humans. Performing steady-state respirometry with isolated mitochondria we found that oxygen limitation of respiration reduced electron flow and oxidative phosphorylation, lowered the mitochondrial membrane potential difference, and decreased mitochondrial calcium influx. Similarly, in myotubes under functional hypoxia mitochondrial calcium uptake decreased in response to sarcoplasmic reticulum calcium release for contraction. In both myotubes and human skeletal muscle this blunted mitochondrial adaptive responses and remodeling upon contractions. Our results suggest that by regulating calcium uptake the mitochondrial electron transfer system is a hub for coordinating cellular adaption under functional hypoxia.<br><br />
|keywords=respirometry; membrane potential; skeletal muscle; exercise; coenzyme Q<br />
|editor=[[Tindle-Solomon L]]<br />
|mipnetlab=AT Innsbruck Oroboros, CH Lausanne Place N, HU Budapest Tretter L<br />
}}<br />
<br />
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-5392-7876]] Donnelly Chris, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-9876-1411]] Komlodi Timea, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-7068-6165]] Cecatto Cristiane, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-6392-9229]] Cardoso Luiza HD, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-9776-7501]] Kayser Bengt, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich,[[File:ORCID.png|20px|link=https://orcid.org/0000-0001-9044-0621]] Place Nicolas<br />
{{Labeling<br />
|area=Respiration, Exercise physiology;nutrition;life style<br />
|injuries=Hypoxia<br />
|organism=Human, Mouse<br />
|tissues=Heart, Skeletal muscle, Nervous system, Other cell lines<br />
|preparations=Isolated mitochondria<br />
|topics=Calcium, mt-Membrane potential, Redox state<br />
|instruments=Oxygraph-2k, TIP2k, O2k-Fluorometer, Ca, NextGen-O2k<br />
|additional=Steady state<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Baglivo_2022_MitoFit-QC&diff=246658Baglivo 2022 MitoFit-QC2024-03-27T07:12:54Z<p>Gnaiger Erich: </p>
<hr />
<div>[[File:Bioblast2022 banner.jpg|link=Bioblast_2022]]<br />
{{MitoFit page name}}<br />
{{Publication<br />
|title=Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. https://doi.org/10.26124/mitofit:2022-0018.v2 — ''2022-08-04 published in [https://doi.org//10.26124/bec:2022-0008 '''Bioenerg Commun 2022.8.''']''<br />
|info=MitoFit Preprints 2022.18. [[File:MitoFit Preprints pdf.png|left|160px|link=https://wiki.oroboros.at/images/c/c8/Baglivo_2022_MitoFit-QC.pdf|MitoFit pdf]] [https://wiki.oroboros.at/images/c/c8/Baglivo_2022_MitoFit-QC.pdf Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry] [[File:WatchThePresentationYoutube_icon.jpg|200px|link=https://www.youtube.com/watch?v=mNSu-hY3hUg&t=15s|»''Watch the presentation''«]]<br />
|authors=MitoFit Preprints<br />
|year=2022<br />
|journal=MitoFit Prep<br />
|abstract=::: <small>Version 2 ('''v2''') '''2022-05-09''' [https://wiki.oroboros.at/images/c/c8/Baglivo_2022_MitoFit-QC.pdf doi:10.26124/mitofit:2022-0018.v2]</small><br />
::: <small>Version 1 (v1) 2022-05-05 [https://wiki.oroboros.at/images/archive/c/c8/20220506062726%21Baglivo_2022_MitoFit-QC.pdf doi:10.26124/mitofit:2022-0018.v1] - [https://wiki.oroboros.at/index.php/File:Baglivo_2022_MitoFit-QC.pdf »Link to all versions«]</small><br />
<br />
[[File:Baglivo 2022 MitoFit QC graphical-abstract.png|right|300px|Graphical abstract]]<br />
<br />
[[Baglivo 2022 Abstract Bioblast]]: Evaluation of instrumental reproducibility is a primary component of quality control to quantify the precision and limit of detection of analytical procedures. A pre-analytical instrumental standard operating procedure (SOP) is implemented in high-resolution respirometry consisting of: (''1'') a daily SOP-POS for air calibration of the polarographic oxygen sensor (POS) in terms of oxygen concentration ''c''<sub>O<sub>2</sub></sub> [µM]. This is part of the ''sensor test'' to evaluate POS performance; (''2'') a monthly SOP-BG starting with the SOP-POS followed by the ''chamber test'' quantifying the instrumental O<sub>2</sub> background. The chamber test focuses on the slope d''c''<sub>O<sub>2</sub></sub>/d''t'' [pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>] to determine O<sub>2</sub> consumption by the POS and O<sub>2</sub> backdiffusion into the chamber as a function of ''c''<sub>O<sub>2</sub></sub> in the absence of sample. Finally, zero O<sub>2</sub> calibration completes the sensor test. <br />
<br />
We applied this SOP in a 3-year study using 48 Oroboros O2k chambers. Stability of air and zero O<sub>2</sub> calibration signals was monitored throughout intervals of up to 8 months without sensor service. Maximum drift over 1 to 3 days was 0.06 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>, without persistence over time since drift was <0.004 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> for time intervals of one month, corresponding to a drift per day of 0.2 % of the signal at air saturation. Instrumental O<sub>2</sub> background -d''c''<sub>O<sub>2</sub></sub>/d''t'' was stable within ±1 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> when measured at monthly intervals. These results confirm the instrumental limit of detection of volume-specific O<sub>2</sub> flux at ±1 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>. The instrumental SOP applied in the present study contributes to the generally applicable internal quality control management ensuring the unique reproducibility in high-resolution respirometry.<br />
|mipnetlab=AT Innsbruck Oroboros<br />
}}<br />
ORC'''ID''':[[File:ORCID.png|20px|link= https://orcid.org/0000-0002-5507-9663]] Baglivo Eleonora [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-6392-9229]] Cardoso Luiza HD, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-7068-6165]] Cecatto Cristiane, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich<br />
__TOC__<br />
== Data availability ==<br />
:::: Original files are available Open Access at Zenodo repository: https://doi.org/10.26124/mitofit:2022-0018<br />
<br />
== References ==<br />
<br />
{{#ask:[[Additional label::MitoFit2022QC]]<br />
| mainlabel=Link<br />
|?Has title=Reference<br />
|?Was published in year=Year<br />
|?Has info=View<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Has title<br />
|order=ascending<br />
}}<br />
<br />
== Support ==<br />
<br />
:::: This work was part of the Oroboros [[NextGen-O2k]] project, with funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement nº 859770.<br />
<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|tissues=HEK<br />
|instruments=Oxygraph-2k, O2k-Protocol<br />
|additional=Bioblast 2022, SUIT-001 O2 ce-pce D003<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=MitoPedia:_DatLab&diff=246647MitoPedia: DatLab2024-03-26T09:27:49Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}{{MitoPedia_navigation_line}}<br />
::: '''Welcome at MitoPedia: DatLab'''. Here you find [[O2k-Open Support|'''Oroboros support''']] for DatLab and the '''[[MitoPedia: O2k hardware |O2k]]'''.<br />
<br />
<br />
<big><big>'''List of DatLab menus'''</big></big><br />
__TOC__<br />
<br />
[[File:DatLab-bar.jpg|460px|link=DatLab]]<br />
[[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]]<br />
<big><big>'''[[DatLab installation]]'''</big></big><br />
::::* 2024-03-21 On the calendar date of the beginning of spring, the Oroboros DatLab team proudly announced that '''the new DatLab 8.1 version was finally released'''.<br />
::::» <big> [[MiPNet21.16 DatLab 7 Innovations]]</big><br />
::::» <big> [[MiPNet26.06 DatLab 7: Guide]]</big><br />
<br />
[[File:File-menu.png|right|150px]]<br />
=== File ===<br />
::::» [[Open DLD file|Open]]<br />
::::» [[Close - DatLab| Close]]<br />
::::» [[Save - DatLab | Save]]<br />
::::» [[Save and Disconnect | Save and disconnect]]<br />
::::» [[Save as - DatLab | Save as]]<br />
::::» [[File search - DatLab| File search]]<br />
::::» [[Delete - DatLab | Delete]]<br />
::::» Import DatLab templates [[DatLab templates]]<br />
::::» Export<br />
:::::* [[Export DL-Protocol User (*.DLPU)]]<br />
:::::* Data to text file: [[Text file - DatLab | Text file]]<br />
:::::* Export - Events to text file: [[Text file - DatLab | Text file]]<br />
:::::* Export - One channel to DatLab 2 analysis: [[DatLab 2]]<br />
::::» Change user: [[User code - DatLab | User]]<br />
::::» Manage users: [[User code - DatLab | User]]<br />
<br />
[[File:O2k-menu.png|right|150px]]<br />
=== Oroboros O2k ===<br />
::::» [[O2k configuration]]<br />
::::» [[O2k channel labels]]<br />
::::» [[O2k control]]<br />
::::» [[Stirrer A on/off]]<br />
::::» [[Stirrer B on/off]]<br />
::::» [[Stirrer test]]<br />
::::» [[Illumination on/off]]<br />
::::» Manage setups: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
<br />
[[File:TIP2k-menu.png|right|150px]]<br />
=== TIP2k ===<br />
::::» TIP2k control: [[MiPNet12.10 TIP2k-manual|Titration-Injection microPump]]<br />
::::» Manage setups: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
<br />
[[File:Protocol-menu.png|right|200px]]<br />
=== Protocols ===<br />
<br />
::::» [[Run DL-Protocol/Set O2 limit]]<br />
::::» [[Run_DL-Protocol/Set_O2_limit#Protocol_menu |Show DL-Protocol and Synchronous DL-Protocol events]]<br />
::::» [[Instrumental: Browse DL-Protocols and templates]]<br />
::::» [[SUIT: Browse DL-Protocols and templates]]<br />
::::» [[Install Oroboros protocol package]]<br />
::::» [[MitoPedia: SUIT |Browse www.bioblast.at/index.php./MitoPedia: SUIT]]<br />
::::» [[Enable DL-Protocol editing]]<br />
<br />
[[File:Experiment-menu.png|right|150px]]<br />
=== Experiment ===<br />
::::» [[Sample - DatLab |Sample]]<br />
::::» [[Experimental log - DatLab |Experimental log]]<br />
::::» Add event: [[Events - DatLab |Events]]<br />
<br />
[[File:Calibration-menu.png|right|150px]]<br />
=== Calibration ===<br />
::::» O2: [[O2 calibration - DatLab|O2 calibration]]<br />
::::» Amp: [[Amp calibration - DatLab|Amp calibration]] <br />
::::» pX: [[PX calibration - DatLab|pX calibration]]<br />
<br />
[[File:Flux-Slope-menu.png|right|130px]]<br />
=== Flux / Slope ===<br />
::::» O2: [[Flux / Slope]]<br />
::::» Amp: [[Flux / Slope]]<br />
::::» pX: [[Flux / Slope]]<br />
::::» Proton [[Flux / Slope]]<br />
<br />
[[File:Graph-menu.png|right|150px]]<br />
=== Graph ===<br />
::::» Add graph/Delete bottom graph: [[Add Graph/Delete bottom graph|Add/Delete]]<br />
::::» Select plots: [[Select plots - DatLab]]<br />
::::» Scaling: [[Scaling - DatLab]]<br />
::::» Info: [[Layout_for_DatLab_graphs#Graph / Info|Graph / Info]]<br />
::::» [[Synchronous time axes]]<br />
::::» [[Autoscale]]<br />
:::::* [[Autoscale time axis]]<br />
:::::* [[Autoscale Y1 (Y2) axes]]<br />
:::::* [[Automatic pan]]<br />
::::» Mouse control: [[Mouse control: Mark|Mark]] and [[Mouse control: Zoom|Zoom]]<br />
::::» [[Full screen]]<br />
::::» [[Display numerical value]]<br />
::::» [[Display Power-O2k]]<br />
::::» Options: [[Graph options - DatLab |Graph options]]<br />
::::» [[Copy to clipboard]]<br />
<br />
<br />
[[File:Layout-menu.png|right|150px]]<br />
=== Layout ===<br />
::::» Info / Load / Save: [[Layout_for_DatLab_graphs#Layout: Info/ Load/ Safe| Layout: Info/ Load/ Safe]]<br />
::::» Standard layouts - O2k-FluoRespirometer: [[Layout_for_DatLab_graphs#Standard layouts - O2k-FluoRespirometer |Standard layouts - O2k-FluoRespirometer]]<br />
::::» Lab layouts: [[Layout_for_DatLab_graphs#Lab layouts| Lab layouts]]<br />
::::» User: Name: [[Layout_for_DatLab_graphs#User: Name| User: Name]]<br />
<br />
[[File:Marks-menu.png|right|150px]]<br />
=== Marks ===<br />
::::» Edit mark information: [[Marks - DatLab|Marks]]<br />
::::» Statistics: [[Mark statistics - DatLab|Mark statistics]]<br />
::::» Manage mark stat. templates: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
::::» Specifications: [[Mark specifications - DatLab |Mark specifications]]<br />
::::» Manage mark specifications templates: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
::::» [[Copy marks]]<br />
<br />
<br />
== DatLab glossary ==<br />
<br />
{{#ask:[[MitoPedia O2k and high-resolution respirometry::DatLab]]<br />
| mainlabel=Term<br />
|?Has abbr=Abbreviation<br />
|?Description=Description<br />
|format=broadtable<br />
|limit=1000<br />
|order=ascending<br />
}}<br />
<br />
<br />
<br />
== Further links ==<br />
<br />
::::» [[O2k-Manual#DatLab_manuals |O2k-Manual: DatLab]]<br />
::::» [[O2k-Demo experiments]]<br />
::::» [[DatLab |O2k-Catalogue: DatLab]]<br />
<br />
<br />
::: Related topics<br />
::::» [[MitoPedia: Respirometry|Respirometry]]<br />
::::» [[MitoPedia: Fluorometry|Fluorometry]]<br />
::::» [[MitoPedia: Spectrophotometry|Spectrophotometry]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Select_O2k_-_DatLab&diff=246645Select O2k - DatLab2024-03-26T08:26:46Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
{{MitoPedia without banner<br />
|description='''Select O2k - DatLab''' <br />
|info=<br />
}}<br />
<br />
__TOC__<br />
== DatLab 8 ==<br />
<br />
:::* 2024-03-21 On the calendar date of the beginning of spring, the Oroboros DatLab team proudly announced that '''the new DatLab 8.1 version was finally released'''.<br />
<br />
<br />
{{Labeling<br />
|area=Instruments;methods<br />
|additional=DatLab, DL8, DatLab 8<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=DatLab<br />
}}<br />
[[Category:DatLab 8]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=DatLab&diff=246644DatLab2024-03-26T08:19:41Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Technical support}}<br />
__TOC__<br />
{{MitoPedia without banner<br />
|abbr=<br />
|description=[[Image:Logo OROBOROS-DatLab.jpg|200px|right|DatLab]]'''DatLab''' is the O2k-Software for Data Acquisition & Analysis, specifically developed for [[high-resolution respirometry]] with the O2k.<br />
The newest DatLab version is '''DatLab 8''', included in the O2k-Packages. NextGen-O2k and O2k-Series J* and higher come with DatLab 8 installed on the integrated PC (Linux). DatLab 8 is required for the NextGen-O2k. DatLab 8.1 is compatible with O2k-Series (E and higher).<br />
<br />
The DatLab software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices.<br />
<br />
The minimum computer requirements are Intel-Core-2 or equivalent CPU, 2GB RAM and Windows XP (64-bit version). However, we recommend Intel i5 or equivalent CPU, 4GB RAM, Windows 10 (64-bit version) and SSD. For the proper display of DatLab on your computer, please make sure the “Language settings” are set to English.<br />
<br />
&#42;Optionally available without integrated PC.<br />
<br />
|info=[[Image:MitoPedia.jpg|left|60px|link=http://www.bioblast.at/index.php/MitoPedia:_DatLab |MitoPedia: DatLab]] [[MitoPedia: DatLab]]<br />
}}<br />
<br><br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F7F8FF"><br />
<span style="font-size:105%; color:#424242">[[File:Questions.jpg|left|18px |Asking]]'''Does [[DatLab]] software run on MAC devices?'''</span><br />
<div class="mw-collapsible-content"> No. The [[DatLab]] software is designed for Windows operating systems.<br />
</div><br />
</div><br />
<br><br />
{{Labeling<br />
|area=Instruments;methods<br />
|instruments=Oxygraph-2k, O2k-Fluorometer<br />
|additional=DatLab<br />
}}<br />
<br />
<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware, DatLab, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Feero_2024_JAMA&diff=246639Feero 2024 JAMA2024-03-25T09:04:38Z<p>Gnaiger Erich: Created page with "{{Publication |title=Feero WG, Steiner RD, Slavotinek A, Faial T, Bamshad MJ, Austin J, Korf BR, Flanagin A, Bibbins-Domingo K (2024) Guidance on use of race, ethnicity, and g..."</p>
<hr />
<div>{{Publication<br />
|title=Feero WG, Steiner RD, Slavotinek A, Faial T, Bamshad MJ, Austin J, Korf BR, Flanagin A, Bibbins-Domingo K (2024) Guidance on use of race, ethnicity, and geographic origin as proxies for genetic ancestry groups in biomedical publications. JAMA https://doi.org/10.1001/jama.2024.3737.<br />
|info=[https://jamanetwork.com/journals/jama/fullarticle/2816403 Open Access]<br />
|authors=Feero WG, Steiner RD, Slavotinek A, Faial T, Bamshad MJ, Austin J, Korf BR, Flanagin A, Bibbins-Domingo K<br />
|year=2024<br />
|journal=JAMA<br />
|abstract=In March 2023, the National Academies of Sciences, Engineering, and Medicine (NASEM) released a consensus study report titled Using Population Descriptors in Genetics and Genomics Research.1 Sponsored by the US National Institutes of Health, the report is more than a discussion of the use of terminology; the authors of the NASEM report suggest a tectonic shift away from current models that use race, ethnicity, and geographic origin as proxies for genetic ancestry groups (ie, a set of individuals who share more similar genetic ancestries) in genetic and genomic science. The recommendations are rooted in evidence that genetic variation in individuals falls, in general, on a continuum of variation not captured well by existing population descriptors and that the ongoing use of such descriptors as analytical variables jeopardizes the scientific validity of research.2 Furthermore, the authors of the NASEM report point out that current scientific practices can sometimes perpetuate harmful typological thinking about individuals, including racism.<br />
|editor=Gnaiger E<br />
}}<br />
{{Labeling<br />
|additional=BEC<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Talk:Metformin&diff=246638Talk:Metformin2024-03-25T08:09:41Z<p>Gnaiger Erich: </p>
<hr />
<div>::::* https://pubmed.ncbi.nlm.nih.gov/28500767/<br />
::::* https://www.frontiersin.org/articles/10.3389/fphys.2022.867244/full?utm_source=F-AAE&utm_medium=EMLF&utm_campaign=MRK_1846266_a0P58000000G0YjEAK_Physio_20220407_arts_A<br />
<br />
::::* Xiao S, Li V, Lyu X et al. Lac-Phe mediates the effects of metformin on food intake and body weight. Nat. Metab. doi:10.1038/s42255-024-00999-9 (2024)</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Talk:Communication_-_mitochondria_and_the_patient&diff=246630Talk:Communication - mitochondria and the patient2024-03-24T19:09:55Z<p>Gnaiger Erich: Created page with "== Links == ::::* World Health Organization (2001) International classification of functioning, disability and health (ICF). World Health Organization, Geneva. https://iris.w..."</p>
<hr />
<div>== Links ==<br />
<br />
::::* World Health Organization (2001) International classification of functioning, disability and health (ICF). World Health Organization, Geneva. https://iris.who.int/bitstream/handle/10665/42407/9241545429.pdf?sequence=1 - [https://wiki.oroboros.at/index.php/World_Health_Organization_2001_ICF »Bioblast link«]<br />
::::* World Health Organization (2010) Framework for Action on Interprofessional Education & Collaborative Practice (IPECP). World Health Organization, Geneva. http://www.who.int/hrh/nursing_midwifery/en/ - [https://wiki.oroboros.at/index.php/World_Health_Organization_2010_IPECP »Bioblast link«] - [https://interprofessional.global/about/interprofessional-education-collaborative-practice/ Interprofessional education and interprofessional collaborative practice IPECP]<br />
::::* https://www.inproproject.eu/</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Communication_-_mitochondria_and_the_patient&diff=246629Communication - mitochondria and the patient2024-03-24T19:09:37Z<p>Gnaiger Erich: </p>
<hr />
<div>{{MitoPedia<br />
|description=Mitochondria and the patient: communication between patients, medical professionals, scientists, and the public<br />
}}<br />
<br />
{{MitoPedia topics<br />
|mitopedia topic=MitoGlobal Organizations<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Communication_-_mitochondria_and_the_patient&diff=246628Communication - mitochondria and the patient2024-03-24T19:08:33Z<p>Gnaiger Erich: Created page with "{{MitoPedia |description=Mitochondria and the patient: communication between patients, medical professionals, scientists, and the public }} {{MitoPedia concepts}} {{MitoPedia..."</p>
<hr />
<div>{{MitoPedia<br />
|description=Mitochondria and the patient: communication between patients, medical professionals, scientists, and the public<br />
}}<br />
{{MitoPedia concepts}}<br />
{{MitoPedia methods}}<br />
{{MitoPedia O2k and high-resolution respirometry}}<br />
{{MitoPedia topics<br />
|mitopedia topic=MitoGlobal Organizations<br />
}}<br />
== Links ==<br />
::::* World Health Organization (2001) International classification of functioning, disability and health (ICF). World Health Organization, Geneva. https://iris.who.int/bitstream/handle/10665/42407/9241545429.pdf?sequence=1 - [https://wiki.oroboros.at/index.php/World_Health_Organization_2001_ICF »Bioblast link«]<br />
::::* World Health Organization (2010) Framework for Action on Interprofessional Education & Collaborative Practice (IPECP). World Health Organization, Geneva. http://www.who.int/hrh/nursing_midwifery/en/ - [https://wiki.oroboros.at/index.php/World_Health_Organization_2010_IPECP »Bioblast link«] - [https://interprofessional.global/about/interprofessional-education-collaborative-practice/ Interprofessional education and interprofessional collaborative practice IPECP]<br />
::::* https://www.inproproject.eu/</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=World_Health_Organization_2010_IPECP&diff=246627World Health Organization 2010 IPECP2024-03-24T18:57:47Z<p>Gnaiger Erich: Created page with "{{Publication |title=World Health Organization (2010) Framework for Action on Interprofessional Education & Collaborative Practice (IPECP). World Health Organization, Geneva...."</p>
<hr />
<div>{{Publication<br />
|title=World Health Organization (2010) Framework for Action on Interprofessional Education & Collaborative Practice (IPECP). World Health Organization, Geneva. http://www.who.int/hrh/nursing_midwifery/en/<br />
|info=[https://interprofessional.global/wp-content/uploads/2018/11/WHO-2010-WHO-framework-for-action-on-interprofessional-education-collaborative-practice.pdf Open Access]<br />
|authors=World Health Organization<br />
|year=2010<br />
|journal=World Health Organization Geneva<br />
|abstract=At a time when the world is facing a shortage of health workers, policy-makers are looking for innovative strategies that can help them develop policy and programmes to bolster the global health workforce. The ''Framework for Action on Interprofessional Education and Collaborative Practice'' highlights the current status of interprofessional collaboration around the world, identifies the mechanisms that shape successful collaborative teamwork and outlines a series of action items that policy-makers can apply within their local health system (Figure 1). The goal of the Framework is to provide strategies and ideas that will help health policy-makers implement the elements of interprofessional education and collaborative practice that will be most beneficial in their own jurisdiction.<br />
<br />
* The World Health Organization (WHO) and its partners recognize interprofessional collaboration in education and practice as an innovative strategy that will play an important role in mitigating the global health workforce crisis.<br />
<br />
* Interprofessional education occurs when students from two or more professions learn about, from and with each other to enable effective collaboration and improve health outcomes.<br />
<br />
* Interprofessional education is a necessary step in preparing a “collaborative practice-ready” health workforce that is better prepared to respond to local health needs.<br />
<br />
* A collaborative practice-ready health worker is someone who has learned how to work in an interprofessional team and is competent to do so.<br />
<br />
* Collaborative practice happens when multiple health workers from different professional backgrounds work together with patients, families, carers and communities to deliver the highest quality of care. It allows health workers to engage any individual whose skills can help achieve local health goals.<br />
<br />
* After almost 50 years of enquiry, the World Health Organization and its partners acknowledge that there is sufficient evidence to indicate that effective interprofessional education enables effective collaborative practice.<br />
<br />
* Collaborative practice strengthens health systems and improves health outcomes.<br />
<br />
* Integrated health and education policies can promote effective interprofessional education and collaborative practice.<br />
<br />
* A range of mechanisms shape effective interprofessional education and collaborative practice. These include: <br />
:- supportive management practices <br />
:- identifying and supporting champions <br />
:- the resolve to change the culture and attitudes of health workers<br />
:- a willingness to update, renew and revise existing curricula <br />
:- appropriate legislation that eliminates barriers to collaborative practice.<br />
<br />
* Mechanisms that shape interprofessional education and collaborative practice are not the same in all health systems. Health policy-makers should utilize the mechanisms that are most applicable and appropriate to their own local or regional context.<br />
<br />
* Health leaders who choose to contextualize, commit and champion interprofessional education and collaborative practice position their health system to facilitate achievement of the health-related Millennium Development Goals (MDGs).<br />
<br />
* The ''Framework for Action on Interprofessional Education and Collaborative Practice'' provides policy-makers with ideas on how to implement interprofessional education and collaborative practice within their current context.<br />
<br><br />
<br />
|editor=Gnaiger E<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=World_Health_Organization_2001_ICF&diff=246626World Health Organization 2001 ICF2024-03-24T16:47:00Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Publication<br />
|title=World Health Organization (2001) International classification of functioning, disability and health (ICF). World Health Organization, Geneva. https://iris.who.int/bitstream/handle/10665/42407/9241545429.pdf?sequence=1<br />
|info=[https://iris.who.int/bitstream/handle/10665/42407/9241545429.pdf?sequence=1 Open Access]<br />
|authors=World Health Organization<br />
|year=2001<br />
|journal=World Health Organization Geneva<br />
|abstract=This volume contains the ''International Classification of Functioning, Disability and Health'', known as ICF. The overall aim of the ICF classification is to provide a unified and standard language and framework for the description of health and health-related states. It defines components of health and some health-related components of well-being (such as education and labour). The domains contained in ICF can, therefore, be seen as ''health domains'' and ''health-related domains''. These domains are described from the perspective of the body, the individual and society in two basic lists: (1) Body Functions and Structures; and (2) Activities and Participation.<sup>2</sup> As a classification, ICF systematically groups different domains<sup>3</sup> for a person in a given health condition (e.g. what a person with a disease or disorder does do or can do). Functioning is an umbrella term encompassing all body functions, activities and participation; similarly, disability serves as an umbrella term for impairments, activity limitations or participation restrictions. ICF also lists environmental factors that interact with all these constructs. In this way, it enables the user to record useful profiles of individuals’ functioning, disability and health in various domains.<br />
<br />
<sup>2</sup> These terms, which replace the formerly used terms “impairment”, “disability” and “handicap”, extend the scope of the classification to allow positive experiences to be described. The new terms are further defined in this Introduction and are detailed within the classification. It should be noted that these terms are used with specific meanings that may differ from their everyday usage.<br />
<br />
<sup>3</sup> A domain is a practical and meaningful set of related physiological functions, anatomical structures, actions, tasks, or areas of life.<br />
|editor=Gnaiger E<br />
}}<br />
== ICF ==<br />
<br />
:::* Towards a common language for functioning, disability and health ICF (2002) WHO/EIP/GPE/CAS/01.3 - [https://cdn.who.int/media/docs/default-source/classification/icf/icfbeginnersguide.pdf Open Access]<br />
<br />
:::: ICF: "It is the conceptual basis for the definition, measurement and policy formulations for health and disability. It is a universal classification of disability and health for use in health and health-related sectors."<br />
<br />
:::: "ICF is named as it is because of its stress is on health and functioning, rather than on disability. Previously, disability began where health ended; once you were disabled, you where in a separate category. We want to get away from this kind of thinking. We want to make ICF a tool for measuring functioning in society, no matter what the reason for one's impairments. So it becomes a much more versatile tool with a much broader area of use than a traditional classification of health and disability. .. This is a radical shift. From emphasizing people's disabilities, we now focus on their level of health."</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=World_Health_Organization_2001_ICF&diff=246625World Health Organization 2001 ICF2024-03-24T16:27:00Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Publication<br />
|title=World Health Organization (2001) International classification of functioning, disability and health. World Health Organization Geneva. https://iris.who.int/bitstream/handle/10665/42407/9241545429.pdf?sequence=1<br />
|info=[https://iris.who.int/bitstream/handle/10665/42407/9241545429.pdf?sequence=1 Open Access]<br />
|authors=World Health Organization<br />
|year=2001<br />
|journal=World Health Organization Geneva<br />
|abstract=This volume contains the ''International Classification of Functioning, Disability and Health'', known as ICF. The overall aim of the ICF classification is to provide a unified and standard language and framework for the description of health and health-related states. It defines components of health and some health-related components of well-being (such as education and labour). The domains contained in ICF can, therefore, be seen as ''health domains'' and ''health-related domains''. These domains are described from the perspective of the body, the individual and society in two basic lists: (1) Body Functions and Structures; and (2) Activities and Participation.<sup>2</sup> As a classification, ICF systematically groups different domains<sup>3</sup> for a person in a given health condition (e.g. what a person with a disease or disorder does do or can do). Functioning is an umbrella term encompassing all body functions, activities and participation; similarly, disability serves as an umbrella term for impairments, activity limitations or participation restrictions. ICF also lists environmental factors that interact with all these constructs. In this way, it enables the user to record useful profiles of individuals’ functioning, disability and health in various domains.<br />
<br />
<sup>2</sup> These terms, which replace the formerly used terms “impairment”, “disability” and “handicap”, extend the scope of the classification to allow positive experiences to be described. The new terms are further defined in this Introduction and are detailed within the classification. It should be noted that these terms are used with specific meanings that may differ from their everyday usage.<br />
<br />
<sup>3</sup> A domain is a practical and meaningful set of related physiological functions, anatomical structures, actions, tasks, or areas of life.<br />
|editor=Gnaiger E<br />
}}<br />
== ICF ==<br />
<br />
:::* Towards a common language for functioning, disability and health ICF (2002) WHO/EIP/GPE/CAS/01.3 - [https://cdn.who.int/media/docs/default-source/classification/icf/icfbeginnersguide.pdf Open Access]<br />
<br />
:::: ICF: "It is the conceptual basis for the definition, measurement and policy formulations for health and disability. It is a universal classification of disability and health for use in health and health-related sectors."<br />
<br />
:::: "ICF is named as it is because of its stress is on health and functioning, rather than on disability. Previously, disability began where health ended; once you were disabled, you where in a separate category. We want to get away from this kind of thinking. We want to make ICF a tool for measuring functioning in society, no matter what the reason for one's impairments. So it becomes a much more versatile tool with a much broader area of use than a traditional classification of health and disability. .. This is a radical shift. From emphasizing people's disabilities, we now focus on their level of health."</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=World_Health_Organization_2001_ICF&diff=246624World Health Organization 2001 ICF2024-03-24T16:17:41Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Publication<br />
|title=World Health Organization (2001) International classification of functioning, disability and health. World Health Organization Geneva. https://iris.who.int/bitstream/handle/10665/42407/9241545429.pdf?sequence=1<br />
|info=[https://iris.who.int/bitstream/handle/10665/42407/9241545429.pdf?sequence=1 Open Access]<br />
|authors=World Health Organization<br />
|year=2001<br />
|journal=World Health Organization Geneva<br />
|abstract=This volume contains the ''International Classification of Functioning, Disability and Health'', known as ICF. The overall aim of the ICF classification is to provide a unified and standard language and framework for the description of health and health-related states. It defines components of health and some health-related components of well-being (such as education and labour). The domains contained in ICF can, therefore, be seen as ''health domains'' and ''health-related domains''. These domains are described from the perspective of the body, the individual and society in two basic lists: (1) Body Functions and Structures; and (2) Activities and Participation.<sup>2</sup> As a classification, ICF systematically groups different domains<sup>3</sup> for a person in a given health condition (e.g. what a person with a disease or disorder does do or can do). Functioning is an umbrella term encompassing all body functions, activities and participation; similarly, disability serves as an umbrella term for impairments, activity limitations or participation restrictions. ICF also lists environmental factors that interact with all these constructs. In this way, it enables the user to record useful profiles of individuals’ functioning, disability and health in various domains.<br />
<br />
<sup>2</sup> These terms, which replace the formerly used terms “impairment”, “disability” and “handicap”, extend the scope of the classification to allow positive experiences to be described. The new terms are further defined in this Introduction and are detailed within the classification. It should be noted that these terms are used with specific meanings that may differ from their everyday usage.<br />
<br />
<sup>3</sup> A domain is a practical and meaningful set of related physiological functions, anatomical structures, actions, tasks, or areas of life.<br />
|editor=Gnaiger E<br />
}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=World_Health_Organization_2001_ICF&diff=246623World Health Organization 2001 ICF2024-03-24T16:15:36Z<p>Gnaiger Erich: Created page with "{{Publication |title=World Health Organization (2001) International classification of functioning, disability and health. World Health Organization Geneva. https://iris.who.in..."</p>
<hr />
<div>{{Publication<br />
|title=World Health Organization (2001) International classification of functioning, disability and health. World Health Organization Geneva. https://iris.who.int/bitstream/handle/10665/42407/9241545429.pdf?sequence=1<br />
|info=[https://iris.who.int/bitstream/handle/10665/42407/9241545429.pdf?sequence=1 Open Access]<br />
|authors=World Health Organization<br />
|year=2001<br />
|journal=World Health Organization Geneva<br />
|abstract=This volume contains the ''International Classification of Functioning, Disability and Health'', known as ICF. The overall aim of the ICF classification is to provide a unified and standard language and framework for the description of health and health-related states. It defines components of health and some health-related components of well-being (such as education and labour). The domains contained in ICF can, therefore, be seen as ''health domains'' and ''health-related domains''. These domains are described from the perspective of the body, the individual and society in two basic lists: (1) Body Functions and Structures; and (2) Activities and Participation.2 As a classification, ICF systematically groups different domains3 for a person in a given health condition (e.g. what a person with a disease or disorder does do or can do). Functioning is an umbrella term encompassing all body functions, activities and participation; similarly, disability serves as an umbrella term for impairments, activity limitations or participation restrictions. ICF also lists environmental factors that interact with all these constructs. In this way, it enables the user to record useful profiles of individuals’ functioning, disability and health in various domains.<br />
<br />
2. These terms, which replace the formerly used terms “impairment”, “disability” and “handicap”, extend the scope of the classification to allow positive experiences to be described. The new terms are further defined in this Introduction and are detailed within the classification. It should be noted that these terms are used with specific meanings that may differ from their everyday usage.<br />
<br />
3. A domain is a practical and meaningful set of related physiological functions, anatomical structures, actions, tasks, or areas of life.<br />
|editor=Gnaiger E<br />
}}<br />
{{Labeling}}</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=Help:Formatting&diff=246622Help:Formatting2024-03-24T08:58:29Z<p>Gnaiger Erich: </p>
<hr />
<div>__TOC__<br />
::::» Cheatsheet: https://en.wikipedia.org/wiki/Help:Cheatsheet<br />
<br />
== General text formatting ==<br />
<br />
* The general text indent should comprise four colons (::::). Make a space after the double points and before the text. Try to divide the text into passages.<br />
* If you create a list with bullets, use three preceding colons (:::*) <br />
* If you create a list with numeration, use four preceding colons (::::#) <br />
<br />
* If you do not want headers to get listed in the table of contents, use big and/ or bold font without any indent. <br />
* Use » before links, when they are not integrated into the text<br />
<br />
== General formatting ==<br />
=== Style ===<br />
:::: ''italic''<br />
:::: '''bold''' => when an article name is first mentioned<br />
<br />
=== Headings ===<br />
`== Main Heading Example ==´<br />
<br />
`=== Sub heading Example ===´<br />
<br />
=== Indent ===<br />
: Left<br />
:: more left<br />
::: even more left<br />
<br />
=== Numbering ===<br />
::::# Numbered list<br />
::::# two<br />
::::# three<br />
<br />
=== Bullet list ===<br />
:::* Bullet list<br />
:::* two<br />
:::* three<br />
<br />
=== Subscript and Superscript ===<br />
:::: ''p''<sub>O<sub>2</sub></sub><br />
:::: Ca<sup>2+</sup><br />
<br />
=== Hyperlinks ===<br />
::::» '''[https://en.wikipedia.org/wiki/Help:Link Formatting: Links]'''<br />
<br />
:::* Do not put a link in the heading text, with few exceptions.<br />
<br />
:::* Internal link<br />
:::: Internal link: [[ATP]]<br />
:::: [[ATP|link to ATP but with different text than internal link]]<br />
<br />
:::* External link ===<br />
:::: External link: [http://en.wikipedia.org/wiki/EGTA_%28chemical%29 Wikipedia] ; there is a space between the weblink and the word, which is shown. Do not forget '''http://''' otherwise the link does not work.<br />
<br />
=== Files ===<br />
::::» ''' [https://en.wikipedia.org/wiki/Help:Files Formatting:Files]'''<br />
<br />
:::* You can upload the following file types on the Bioblast wiki: zip, dld, dlt, pdf, png, gif, jpg, jpeg, doc, docx, xls, xlsx <br />
:::: See above the guideline on how to implement files into the website<br />
<br />
== Tables ==<br />
<br />
::::» '''[https://en.wikipedia.org/wiki/Help:Table Formatting: Tables]'''<br />
<br />
<br />
{|border="1"<br />
|+<br />
!Obst<br />
!Gemüse<br />
|-<br />
|align="right"|Banane<br />
|align="right"|Tomate<br />
|-<br />
|align="right"|Birne<br />
|align="right"|Gurke<br />
|-<br />
|}<br />
<br />
<br />
<br />
{|border="5"<br />
|- style="font-style:italic; color:green;"<br />
|align="right"|Banane || align="right"|Birne<br />
|- style="font-style:italic; color:red;"<br />
|align="right"|Tomate || align="right"|Gurke<br />
|-<br />
|}<br />
<br />
<br />
<br />
{|border="1" cellpadding="20" cellspacing="0"<br />
!Shopping list<br />
|-<br />
|<br />
*Milk<br />
*Bread<br />
*Juice<br />
|}<br />
<br />
== Automatic tables ==<br />
<br />
:::: If you want to create an automatic table you have to write down the next code (adapting it to your necessities), after the code you would find the explanation to each part. <br />
<br />
:::: <nowiki><br />
{{#ask:[[Category:O2k-Workshops]] [[Additional label::IOC]] |?Has title=Reference |format=broadtable |limit=500 |offset=0 |sort=Was written by |order=descending}} </nowiki><br />
<br />
::*'''<nowiki>#ask:[[Category:xxx]]</nowiki>''' will search on the category where the information is, you can search over more than one category, for that you have to write:'''<nowiki>#ask:[[Category:xxx]][[Category:xxx]]</nowiki>'''<br />
<br />
::*'''<nowiki>[[Additional label::xxx]]</nowiki>''' in this area you have to write what you want to search, in which field, for example those pages that in the keyword field contein O2k and are from year 2012 -->'''<nowiki>[[Keyword::O2k]][[Year::2012]]</nowiki>'''<br />
<br />
::*'''|?Has title=reference |?Was published in year |?Diseases''' With the question mark you selected the fields you want to appear in the table<br />
<br />
::*'''|format=broadtable |limit=500 |offset=0''' this line define the format of the table. it´s necessary to write limit 500 because if it´s too short it would not appear all of them. <br />
<br />
::*'''|sort= Was written by |sort=was published in year |sort=Has title |sort=Was submitted in year''' Here you selected the field from which the table will be sorted. <br />
<br />
::*'''|order=descending or ascending}}''' choose one of both options to order the table.<br />
<br />
:::: '''Attention-''' <br />
::::* For automatic publication tables '''sort by''' Was published in year / for automatic abstract tables '''sort by''' Was submitted in year<br />
::::* If in the pages where you are searching to create the automatic table, exist the label '''articletype''', this page WILL NOT appear in the table.<br />
<br />
== Figures, Pictures, Images ==<br />
::::» '''[https://www.mediawiki.org/wiki/Help:Images Formatting:Images]'''<br />
<br />
:::: Upload: Go to 'Upload file' in the 'Toolbox' or just click this link: [[Special:Upload]]<br />
:::: [http://en.wikipedia.org/wiki/Help:Wiki_markup#Images Figures, Images] Instructions to insert the picture on the page can be found on the 'Upload file' page.<br />
:::: [[Special:ListFiles]]<br />
<br />
::::* Uploaded pictures should not have more than 1200px on the longest side. Profile pictures of persons should have this ratio: 3:4.<br />
<br />
::::* Several figures do not change size when the zoom of the screen is modified, hence they change in proportion with the text. In many cases, a zoom of 125% is considered as a standard [Ctrl-O]. Therefore, formatting of such figures should be optimized at a zoom of 125%.<br />
<br />
== Side bar ==<br />
::::* [[MediaWiki:Sidebar]]<br />
<br />
== Startpage ==<br />
:::: Changing the startpage of the wiki/ the link of the logo in the navigation bar: Edit the page “MediaWiki:Mainpage”, write inside the name of the page you want to have as the startpage. Save.<br />
<br />
:::: Link: "skins/Vector.php"<br />
<br />
<br />
== Forms ==<br />
::::* [[Special:Forms]]<br />
::::* Linking in Forms<br />
::::# MitoPedia, Publications, MiPnetLabs<br />
:::: when, you work with the Form then the external links [ ] and internal links [[ ]] function as mentioned before<br />
<br />
::::# Products<br />
:::: here in the Info field ONLY internal links (for convenience) will work (since the form automatically adds the wikitag [[ ]] to the references.<br />
<br />
== Mathematical formula ==<br />
<br />
::::''More details:'' » [http://de.wikipedia.org/wiki/Hilfe:TeX Help with MathFormulas]<br />
<br />
== References/Footnotes ==<br />
::::* »[[Bioblast-references]]<br />
:::: Use the following comands within the text: <ref><br />
:::: Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 [[Gnaiger 2020 BEC MitoPathways | »Bioblast link]]</ref>. Then a list of references is generated like this:<br />
<br />
=== References ===<br />
:::: <references/><br />
<br />
== Email ==<br />
<br />
[[Has mailaddress::{{{mailaddress|instruments@oroboros.at}}}]]<br />
<br />
<br />
<br />
[[Category:Help]]</div>Gnaiger Erichhttps://wiki.oroboros.at/index.php?title=National_Academies_of_Sciences,_Engineering,_and_Medicine_2023_BMI_and_beyond&diff=246621National Academies of Sciences, Engineering, and Medicine 2023 BMI and beyond2024-03-24T08:52:20Z<p>Gnaiger Erich: </p>
<hr />
<div>{{Publication<br />
|title=National Academies of Sciences, Engineering, and Medicine (2023) BMI and beyond: Considering context in measuring obesity and its applications: Proceedings of a workshop—in brief. The National Academies Press, Washington, DC. https://doi.org/10.17226/27185<br />
|info=[https://nap.nationalacademies.org/catalog/27185/bmi-and-beyond-considering-context-in-measuring-obesity-and-its?utm_source=NASEM+News+and+Publications&utm_campaign=cae440fb22-EMAIL_CAMPAIGN_2023_07_31_04_08&utm_medium=email&utm_term=0_-cae440fb22-%5BLIST_EMAIL_ID%5D&mc_cid=cae440fb22&mc_eid=50f76db947 Open Access]<br />
|authors=National Academies of Sciences, Engineering, and Medicine<br />
|year=2023<br />
|journal=National Academies Press<br />
|abstract=The Roundtable on Obesity Solutions of the Health and Medicine Division of the National Academies of Sciences, Engineering, and Medicine held a virtual public workshop, BMI and Beyond: Considering Context in Measuring Obesity and its Applications, on April 4, 2023. The workshop was the first in a two-part series to explore the current science on measures of body composition, body fat distribution, and obesity.<br />
|editor=Gnaiger E<br />
}}<br />
----<br />
{{BME_navigation_line}}<br />
__TOC__<br />
<br />
== From BMI to BME ==<br />
''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''mitObesity''']].<br />
<br />
{{Template:Publications: BME and body fat}}<br />
{{Template:Publications: BME and height}}<br />
<br />
{{MitoPedia: BME and mitObesity}}<br />
<br />
{{Labeling<br />
|area=Exercise physiology;nutrition;life style<br />
|diseases=Obesity<br />
|organism=Human<br />
|tissues=Fat<br />
|additional=BMI, BME, Fat<br />
}}</div>Gnaiger Erich